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Surface Temperature and Heat Transfer between Body Regions of Africanized Honeybees(Apis mellifera L.)in Hives under Sun and Shade Conditions in the Northeastern Semi-arid Region of Brazil

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Journal of Agricultural Science and Technology(A) 2018年第1期8卷 28-35页

作者： Herica Girlane Tertulino Domingos Daiana da Silva Sombra ricardo goncalves Santos KátiaPeresGramacho and Lionel Segui goncalves Department of Animal Science The Federal Rural University of the Semi-arid RegionMossoró 59614-290RNBrazil Department of Zootechny The Federal Rural University of the Semi-arid RegionMossoró 59614-290RNBrazil Department of Biology FFCLRPUniversity of Sao PauloRibeirao Preto 14040-080Sao PauloBrazi

This work was done at the experimental station of the Federal Rural University of the Semi-arid Region （UFERSA） in Mossoró, Brazil. This work aimed to evaluate the surface temperature of the bees and heat transfer between body regions of Africanized honeybees （Apis mellifera L.） in hives under sun and shade conditions in the northeastern semi-arid region of Brazil. Twelve colonies of Africanized honeybees housed in Langstroth hives were used. The body surface temperature of the bees was measured using a mini infrared thermometer （Fluke model 62）. The climatological data were obtained through a meteorological station. The comparison of means was performed by the Tukey-Kramer test （p 〈 0.01）, and data analysis using the Statistical Analysis System software. The body temperatures of the bees are not homogeneous and fluctuate throughout the day, in accordance with the environmental variations. The temperature of the thorax is generally higher, followed by the head and abdomen. The head and the abdomen are parts of the body for which the excess temperature of the thorax is transferred, with the head being the main one. The bees that were in the shade, managed to keep their body temperatures at relatively normal levels, while the bees that were in the sun, had a considered increase of its surface body temperatures higher than 2 °C, being necessary the activation of mechanisms of heat loss, and consequently there is a deviation of energy that could be used for other activities within the colony.

关键词： Surface temperature shading thermal comfort Apis mellifera

KIR and a specific HLA-C gene are associated with susceptibility and resistance to hepatitis B virus infection in a Brazilian population

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Cellular & Molecular Immunology 2014年第6期11卷 609-612页

作者： Patricia Araujo Giovana goncalves Flavia Latini Orlando Ferreira Luis Cristovao Porto Jose Augusto Barreto Manoel Joao Castelo Girao ricardo Sobhie Diaz Colsan - Associacao Beneficente de Coleta de Sangue Sao Paulo SP Brazil Department of Gynecology Federal University ofao Paulo (UNIFESP) Sao Paulo SP Brazil Molecular Virology Laboratory Biology Institute Federal University of Rio de Janeiro (UERJ) Rio de Janeiro R J Brazil Histocompatibility and Cryopreservation Laboratory Federal University of Rio de Janeiro (UERJ) Rio de Janeiro R J Brazil Retrovirology Laboratory Federal University of Sao Paulo (UNIFESP) Sao Paulo SP Brazil

BRIEF REPORT The activity of natural killer （NK） cells is partially regulated by killer cell immunoglobulin-like receptors （KIRs） interacting with human leukocyte antigen C （HLA-C） ligands.1 The ligands of sever... 详细信息

关键词： infection Brazilian population

Historicity： Preservation or Revitalization Planning Tools？

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Journal of Civil Engineering and Architecture 2013年第10期7卷 1196-1202页

作者： Mariana Seara Paixao Antonio ricardo da Costa Jorge goncalves Instituto Superior Tecnico Universidade Tecnica de Lisboa Lisboa 1049-001 Portugal

The historic centres revitalization addresses the challenges related to the preservation of fundamental heritage values. At a time, when everyone looks with concern to our cities＇ future, it is important to reflect on the received heritage, seeking the most appropriate answers to the planning of the historic centres. These fabrics are reference places in the urban space, due to their role of memorial testimony and of generators of cultural and economic dynamics. However, often times, inherited urban fabrics are affected by the limitations of the heritage policies which, for being too general and based on theoretical and abstract frameworks, have difficulty incorporating the characteristics of each area and neglect the formulation of specific criteria and intervention methods. The purpose of this paper is to provide a comparative reading of the levels of urban renewal allowed by the planning tools. This study chooses two historic centers in Portugal： Oporto and Guimarfies historic centres （World Heritage Sites since 1996 and 2001, respectively, and were the last to get this classification in Portugal）. This reflection is a contribution to peer trends and raise the discussion on the role that the different heritage policies have to the revitalization of the historic centres.

关键词： Historical centre planning tool urban renewal heritage urban planning.

Expression of α_(6) integrin subunit in bovine oocyte and its potential role during fertilization

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Advances in Bioscience and Biotechnology 2013年第5期4卷 617-622页

作者： Roseli Fernandes goncalves ricardo Pimenta Bertolla Cassia Maria Barroso Orlandi Valquiria Hyppolito Barnabe Department of Animal Reproduction College of Veterinary Medicine and Animal ScienceSao Paulo UniversitySao PauloBrazil Department of Surgery Division of UrologyHuman Reproduction SectionSao Paulo Federal UniversitySao PauloBrazil Laboratory of Animal Endocrinology College of Veterinary MedicineSao Paulo State UniversityAracatubaBrazil

Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.

关键词： Integrins Bovine Oocytes Fertilization Sperm-Oocyte Binding

Contribution of the Incorporation of Poultry Litter and Limestone for the Fertility of Sandy Soil in the Brazilian Amazon

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Natural Resources 2014年第15期5卷 958-967页

作者： Juliana Guimaraes Gerola Juan ricardo Rocha Stella Cristiani goncalves Matoso Leandro Dias da Silva Agricultural Engineering Federal Institute of RondoniaCampus Colorado do OesteColorado do OesteBrazil Biodiversity and Biotechnology of the Amazon at the BIONORTE Network ManausBrazil Soil Science Federal Institute of RondoniaCampus Colorado do OesteColorado do OesteBrazil Soil Laboratory Federal Institute of RondoniaCampus Colorado do OesteColorado do OesteBrazil

Sandy soils have limitations for growing plants. In general, they have low fertility, high toxic aluminum content and low organic matter. The organic matter is responsible for most of the cation exchange capacity of these soils. Added to this, the low clay content and the structure of these soils with large volumes of macropores, determine their low water retention. An alternative is the incorporation of organic waste combined with the practice of liming. Thus, the aim of this study was to evaluate the contribution of the incorporation of poultry litter and limestone in the fertility of sandy soils in the Brazilian Amazon. The experiment was conducted in Colorado do Oeste, RO, on a Quartzipsamment in a completely randomized block design in a 4 × 4 × 2 factorial scheme, corresponding to four doses of poultry litter (0, 5, 10 and 15 mg·ha-1), its incorporation for four times (0, 75, 110 and 145 days) in the presence and absence of 2 mg·ha-1 of limestone, with six replications. The incorporation of 15 mg·ha-1 of poultry litter combined with 2 mg·ha-1 of limestone for 75 days after the merger raises several components of Quartzipsamment fertility, mainly being the availability of phosphorus.

关键词： Quartzipsamments Organic Waste Liming

Intraoperative Glycaemia Following Paracetamol with and without Glucose: A Randomized-Controlled Trial

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International Journal of Clinical Medicine 2013年第9期4卷 409-416页

作者： ricardo Mota Pereira Fatima goncalves Joao Costa Filomena Couto Carolina Sa Isabel Neves Lucindo Ormonde Department of Anaesthesiology Santa Maria University HospitalLisbonPortugal Laboratory of Clinical Pharmacology and Therapeutics Faculty of MedicineUniversity of LisbonLisbonPortugal Evidence-Based Medicine Centre Faculty of MedicineUniversity of LisbonLisbonPortugal Instituto de Medicina Molecular(IMM) Faculty of MedicineUniversity of LisbonLisbonPortugal Department of Gynaecology Santa Maria University HospitalLisbonPortugal

Background: Hyperglycaemia is conversely a risk factor for perioperative complications. We are currently using a generic 3.3 g glucose containing formula of intravenous 1000 mg paracetamol for perioperative analgesia. Our main goal was to compare the trends of glycaemic values after administration of a generic 3.3 g glucose containing formula with a non-glucose containing branded formula of intravenous 1000 mg paracetamol. Methods: A exploratory proof-of-concept randomized clinical trial was conducted with 150 patients scheduled for elective gynaecologic. Patients were randomly assigned into three groups: control group (saline);active-control group: intraoperative administration of a branded non-glucose containing 1000 mg paracetamol formula;experimental group: intraoperative administration of a generic 3.3 g glucose containing 1000 mg paracetamol formula. The primary outcome was mean change from baseline in glaucoma. In case significant differences were found, the following secondary outcomes were explored: the proportion of patients with high glycaemia values (>150 mg/dL) and the proportion of patients with negative glycaemic variation. Results: Mean glycaemia change was higher after generic 3.3 g glucose containing paracetamol formula both in comparison to placebo (16.3 mg/dL [95% CI: 6.1 to 26.6]) and active-control (19.1 mg/dL [8.2 to 30.0] groups. Similar results were found in the intention-to-treat analysis. In only the experimental group, patients had high glycaemic values (11.3%). Conclusions: This study showed that in non-diabetic, under non-cardiac surgery, administration of a generic glucose-containing formula of intravenous 1000 mg paracetamol was associated with poorer glycaemic control. These results raise the question of a possible increased risk among these patients. Further studies using diabetic patients are recommended.

关键词： Paracetamol Hyperglycaemia Gyneacologic Surgical Procedures Randomized Controlled Trial

Low-level laser therapy enhances the number of osteocytes in calvaria bone defects of ovariectomized rats

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Animal Models and Experimental Medicine 2019年第1期2卷 51-57页

作者： Priscilla Hakime Scalize Luiz Gustavo de Sousa Lígia Maria Napolitano goncalves Dimitrius Leonardo Pitol Marcelo Palinkas Antonio Augusto Coppi Mariah Acioli Righeti Vitória ricardo Karina Fittipaldi Bombonato-Prado Simone Cecílio Hallak Regalo Selma Siessere Department of Morphology Physiology and Basic PathologySchool of Dentistry of Ribeirao PretoUSP-University of Sao Paulo Faculty of Health and Medical Sciences School of Veterinary MedicineUniversity of Surrey

Background : Osteoporosis can make bone repair difficult. Low-level laser therapy( LLLT) has been shown to be a promising tool for bone neoformation. This study aimed to analyze the effect of LLLT on calvaria bone defects of ovariectomized rats using stereology. Methods : Fifty-four Wistar rats were subjected to bilateral ovariectomy, and bone defects were created in calvaria after 150 days. The animals were divided into nine groups(n = ?6 per group), and 24 hours after the bone defects were created they received three, six or 12 sessions of LLLT at 0, 20 or 30 J/cm 2, using a 780-nm low-intensity GaAlAs laser. One-way ANOVA followed by Tukey ' s post hoc test was used for data processing. A difference of P < 0.05 was considered statistically significant. The parameters evaluated were osteocyte density( Nv_(ost)), total osteocyte number( Nto ost), trabecular surface density( Sv_t), and trabecular surface area( Sa_t). Results : Data obtained showed that Nto ost, Sv t, and Sa t in group G2 rats were significantly different from G1(0 J/cm^2)( P < 0.05). Compared to group G4, G5 presented higher values for the parameters Sv t and Sa t, and G6 presented significantly higher values for almost all the analyzed parameters( Nv _(ost), Nto_(ost), Sv_t, and Sa t)( P < 0.05). Compared to group G7, G8 showed a higher value only for the parameter Sa t, and G9 showed significantly higher values for parameters Nv ost, Nto ost, Sv_t, and Sa_t. Conclusion : We conclude that LLLT stimulated bone neoformation and contributed to an increase in the total number of osteocytes, especially with a laser energy density of 30 J/cm^2 given for six and 12 sessions.

关键词： bone low-level laser therapy osteocytes osteoporosis

Analysis of Haloacetic Acids(HAA5)in Waters of Public Schools and Residencies in Maringa-Brazil

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Journal of Water Resource and Protection 2018年第11期10卷 1083-1089页

作者： Daniela Jandussi Palumbo ricardo Andreola Mariza Akiko Utida Thaise Moser Teixeira Edison Schmidt Filho Jussara ricardo de Oliveira Aline Maria Orbolato goncalves Zuliani Ariana Ferrari Valmir Tadeu Fernandes Centro Universitário de Maringá(UNICESUMAR) MaringáBrazil

For water to become suitable for human consumption in most water treatment plants this occurs by making use of the chlorination process where the organic matter is destroyed by the action of chlorine. Chlorine is a disinfectant that at low concentrations meets requirements such as not being toxic to humans and inactivating microorganisms. The reaction of chlorine with organic compounds results in chlorination byproducts, many potentially harmful to human health, such as trihalomethanes, haloacetic acids, among others. The present work aimed to collect and analyze samples of treated water from reservoirs from public schools in the city of Maringá, Brazil. Analyses of haloacetic acids (HAA5: monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, bromoacetic acid and dibromoacetic acid), natural organic compounds and free residual chlorine were performed (the latter analysis, in loco). The water collection points were chosen in order to maximize the concentration of haloacetic acids that characterize network distant points from treatment station and also samples near the water treatment plant. With the results, the formation of haloacetic acids between the entrance water of the school and the water of the reservoir of the collection points were compared, where higher values were obtained in the reservoirs. Furthermore, the haloacetic acid levels of water supplied to the population close to the treatment station and distant points of the treatment station were compared, resulting in larger values at the distant points. The value of 0.170 mg/L in haloacetic acid (the maximum value allowed by the legislation is 0.080 mg/L) was obtained at a point distant in the network from the treatment plant. The Consolidation Ordinance n.5/2017, current legislation for treated water in Brazil, was used in relation to the maximum allowable values for free residual chlorine and haloacetic acids.

关键词： Chloro Disinfection Natural Organic Compounds Byproducts

Influence of Quince Rootstocks on Entomosporium Leaf Spot （Entomosporium mespili） Susceptibility in European Pear cv. Abate Fetel

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Journal of Agricultural Science and Technology(B) 2014年第2期4卷 141-149页

作者： Mayra Juline goncalves Amauri Bogo ricardo Trezzi Casa Leo Rufato Jane Nicklin Betina Pereira deBern Aike Anneliese Kretzschmar Femanda Grimaldi Department of Agronomy University of Santa Catarina State/UDESC Lages/SC CEP. 88.p520-000 Santa Catarina State Brazil Department of Biological Science Birkbeck College London WCIE 7HX UK

Entomosporium leaf spot （ELS） is caused by the fungus Fabraea maculata （anamorph： Entomosporium mespili） and affects most pear cultivars and quince rootstocks in Brazil. The aim of this study was to characterize the effect of Adams, EMA and EMC quince rootstocks on ELS in European pear cultivar ＂Abate Fetel＂ in Southern Brazil, during the 2009/2010, 2010/2011 and 2011/2012 growing season. The incidence and severity of disease was quantified weekly in 100 randomly leaves distributed in four medium-height branches per plant with eight replications. Disease progress curves of ELS were constructed and the epidemics compared according to：（1） the beginning of symptoms appearance （BSA）; （2） the time to reach the maximum disease incidence and severity （TRMDI and TRMDS）; （3） area under the incidence and severity disease progress curve （AUIDPC and AUSDPC）. The data were analyzed by linear regression and adjusted for three empirical models： Logistic, Monomolecular and Gompertz. The Abate Fetel cultivar under all rootstocks evaluated was susceptible to E. mespili. However, there were significant differences in ELS intensity among rootstocks evaluated. The highest ELS intensities were observed in combinations with EMA and Adams quince rootstock. Abate Fetel cultivar grafted on EMC quince rootstock showed all epidemiological variables results significantly different when compared with EMA quince rootstock. EMC quince rootstock induced late resistance compared with the other considerated rootstocks. The Logistic model was the most appropriates to describe the ELS progress of Abate Fetel cultivar under all rootstocks evaluated in the edafoclimatic conditions of Southern Brazil, during the 2009/2010, 2010/2011 and 2011/2012 growing season.

关键词： Epidemic epidemiological models Entomosporium mespili Pyrus communis.