Expression of α_(6) integrin subunit in bovine oocyte and its potential role during fertilization

作     者：Roseli Fernandes Goncalves Ricardo Pimenta Bertolla Cassia Maria Barroso Orlandi Valquiria Hyppolito Barnabe 

作者机构：Department of Animal ReproductionCollege of Veterinary Medicine and Animal ScienceSao Paulo UniversitySao PauloBrazil Department of SurgeryDivision of UrologyHuman Reproduction SectionSao Paulo Federal UniversitySao PauloBrazil Laboratory of Animal EndocrinologyCollege of Veterinary MedicineSao Paulo State UniversityAracatubaBrazil 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2013年第4卷第5期

页      面：617-622页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Integrins Bovine Oocytes Fertilization Sperm-Oocyte Binding 

摘      要：Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.