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Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue

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Journal of Biomedical Science and Engineering 2013年第12期6卷 1178-1185页

作者： Julio Cesar Francisco ricardo Correa cunha Rossana Baggio Simeoni Luiz Cesar Guarita-Souza Reginaldo Justino Ferreira Ana Carolina Irioda Carolina Maria C.Oliveira Souza Garikipati Venkata Naga Srikanth Soniya Nityanand Juan Carlos Chachques Katherine Athayde Teixeira de Carvalho Bioprocess Engineering and Biotechnology Department Federal University of ParanáCuritibaBrazil Cell Therapy and Biotechnology in Regenerative Medicine Research Group Pele Pequeno Príncipe InstituteCuritibaBrazil Experimental Laboratory of Institute of Biological and Health Sciences Pontificial Catholic University of Paraná(PUCPR)Rua Imaculada ConceicaoCuritibaBrazil Federal University of Technology CuritibaBrazil Department of Hematology Stem Cell Research FacultySanjay Gandhi Post-Graduate Institute of Medical SciencesLucknowIndia Laboratory of Biosurgical Research Pompidou HospitalUniversity of Paris DescartesParisFrance

Existing therapies for the treatment of chronic heart failure still have some limitations and there is a pressing need for the development of new therapeutic modalities. The amniotic membrane has been used for the treatment of various diseases, such as conjunctive defects;however, the mechanisms behind its repair functions are still unclear. Regenerative medicine is seeking newer alternatives and among them, biomaterials have emerged in recent years for developing and manipulating molecules, cells, tissues or organs grown in laboratories in order to replace human body parts. Many such materials have been used for this purpose, either synthetically or biologically, in order to provide new medical devices. This review provides a wider view of the regeneration potential of the use of amniotic membrane as a potential biomaterial to facilitate the implementation of new research in surgical procedures. Amniotic membrane appears to be an alternative source of stem cells as well as an excellent biomaterial for cell-based therapeutic applications in engineering heart tissue.

关键词： Amniotic Membrane Heart Tissue Engineering Stem Cells

The Impact of Fibromyalgia on Daily Women: A Contribution to Nursing Assistants

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Journal of Biomedical Science and Engineering 2019年第3期12卷 197-200页

作者： Gabriela Carneiro Ana Paola Geronasso Maria Elisa Brum do Nascimento Luiz Claudio Sobrinho do Nascimento Denise Faucz Kletemberg Giovanna Batista Leite Veloso ricardo C. cunha Luiz Cesar Guarita-Souza Rossana B. Simeoni Julio C. Francisco Kátia Renata Antunes Kochla School of Nursing & Health Studies Positivo University Curitiba Brazil School of Medical Sciences Pontifical Catholic University of Paraná Curitiba Brazil The Paraná Institute of Technology Curitiba Brazil

Objective: To evaluate the impact of fibromyalgia in daily and associated factors in women treated at the polyclinic in Paraná. Method: A descriptive, cross-sectional study was conducted with individualized data for 52 women ≥ 50 years from the policlinic of the Curitiba of Paraná State, Brazil. Data were collected an online survey and the quality of life was assessed by one specific instrument, the Fibromyalgia Impact Questionnaire (FIQ). Verbal or written informed consent was obtained from all participants. Results: A total of 157 patients completed the survey. The average age was approximately 50 years (range 29 - 77 years). The most frequent comorbidities were depression (61.5%) and (19%) arthrosis. Conclusion: The understanding of the commodities associated with fibromyalgia helps health professionals to reach the patient efficiently in their orientations about nursing care in women with fibromyalgia.

关键词： Fibromyalgia Depression Nursing Pain Quality of Life

Acellular Amniotic Membrane Patch Regeneration for Burn Injury: The Future for Wound Healing

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Journal of Biomedical Science and Engineering 2019年第8期12卷 409-413页

作者： Tayná Dias Kalyane Machado Kátia Renata Antunes Kochla ricardo cunha Marco André Cardoso Rossana Baggio Simeoni Vinicius Coneglian Santos Péricles Varella Gomes Luiz Claudio Sobrinho do Nascimento Maria Elisa Brum do Nascimento Luiz Cesar Guarita-Souza Alessandro Brawerman Julio Cesar Francisco School of Health Sciences Positivo University Curitiba Brazil School of Medical Sciences Pontifical Catholic University of Paraná Curitiba Brazil The Paraná Institute of Technology Curitiba Brazil Gel Lab Michigan State University East Lansing USA

Acellular amniotic membrane (AAM) is a biodegradable and biocompatible natural that has a wide range of applications in the field of pharmaceutics, biomedical, chemical and cosmetics industry. One of the most interesting characteristics of AAM is its antibacterial and anti-inflammatory, anti-angiogenic, anti-fibrosis activity is an attractive approach for treating inflammatory disorders, wounds, and burns. The isolated use of AAM may not be sufficient to produce an adequate anti-inflammatory and antimicrobial effect to fulfill different purposes, has attracted considerable attention that has similar properties to the native tissues. This mini-review offers a focuses on modern aspects of the biomaterials, growth factors, biocompatibility, and acellular matrix which are the basic elements for use in the tissue regeneration and replacement after an injury.

关键词： Skin Regeneration Amniotic Membrane Wound Healing Tissue Engineering

The influence of porosity on tablet subdivision

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Particuology 2020年第6期18卷 192-196页

作者： Maira T.Teixeira Livia L.Sa-Barreto Izabel C.Silva Tais Gratieri Guilherme M.Gelfuso ricardo N.Marreto Mariana Landin Marcilio cunha-Filho Laboratory of Food Drugs and Cosmetics(LTMAC)University of Brasilia(UnB)70910-900 BrasiliaDFBrazil Laboratory of Nanosystems and Drug Delivery Devices(NanoSYS) Federal University of GoiasGoianiaGO.Brazil Departamento de Farmacologia Farmacia y Tecnologia Farmaceutica1+D Farma Group(Gl-1645)Universidade de Santiago de CompostelaSpain

A tablet microstructure,especially the porosity,is a crucial parameter that influences the mechanical ***,tablet subdivisions were studied as a functi on of tablet *** tablets were manufactured in the presenee of different diluents,namely microcrystalline cellulose,LudipressR,or lactose ***,the add让ion of Camphor was investigated,which was thereafter sublimated with a view of obtaining tablets having varying degrees of *** assays were corre lated to the subdivision performs *** increase in porosity reduced the hardness and increased the tablet friability,adversely impacting the *** all the excipients,an increase in relative porosity>90%represented the threshold level from which an inadequate subdivision *** in crease in tablet porosity led to a reduction in mechanical resista nee,which,combined with a heterogeneous and disc on tinuous distribution of pores within the matrix,resulted in poor subdivision *** trolling tablet porosity is an important consideration when designing tablets having a subdivision purpose.

关键词： Tablet subdivision Porosity Mass variation Mass loss Friability Hardness

Estimation of Evapotranspiration by Various Net Radiation Estimation Formulae for Non-Irrigated Grass in Brazil

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Journal of Water Resource and Protection 2014年第15期6卷 1425-1436页

作者： Antonio Ribeiro da cunha Edgar ricardo Schoffel Clovis Alberto Volpe School of Agronomic Sciences Sao Paulo State University(UNESP)BotucatuBrazil Eliseu Maciel School of Agronomy Federal University of PelotasPelotasBrazil

The objective of this study was to assess the accuracy of estimating evapotranspiration (ET) using the FAO-56 Penman-Monteith (FAO-56-PM) model, with measured and estimated net radiation (Rnmeasured and Rnestimated, respectively), the latter obtained via five different models. We used meteorological data collected between August 2005 and June 2008, on a daily basis and on a seasonal basis (wet vs. dry seasons). The following data were collected: temperature;relative humidity;global global solar radiation (Rs);wind speed and soil heat flux. The atmospheric pressure was determined by aneroid barograph, and sunshine duration was quantified with a Campbell-Stokes recorder. In addition to the sensor readings (Rnmeasured), five different models were used in order to obtain the Rnestimated. Four of those models consider the effects of cloud cover: the original Brunt model;the FAO-24 model for wet climates;the FAO-24 model for dry climates, and the FAO-56 model. The fifth was a linear regression model based on Rs. In estimating the daily ET0 with the FAO-56-PM model, Rnmeasured can be replaced by Rnestimated, in accordance with the FAO-24 model for dry climates, with a relative error of 2.9%, or with the FAO-56 model, with an error of 4.9%, when Rs is measured, regardless of the season. The Rnestimated obtained with the fifth model has a relatively high error. The original Brunt model and FAO-24 model for wet climates performed more poorly than did the other models in estimating the Rn and ET0. In overcast conditions, the original Brunt model, the FAO-24 model for wet climates, the FAO-24 model for dry climates, the FAO-56 model and the model of linear regression with Rs as the predictor variable tended to overestimate Rn and ET, those estimates becoming progressively more accurate as the cloud cover diminished.

关键词： Evapotranspiration Net Radiation Solar Radiation Cloud Cover Empirical Models

Influence of the Chemical Composition of Completion Fluids on the Propagation of Electromagnetic Waves within Oil Wells

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Engineering（科研） 2012年第12期4卷 966-971页

作者： Alexandre Ashade Lassance cunha Marco Aurélio Pacheco José ricardo Bergmann Departamento de Engenharia Elétrica Pontifícia Universidade Católica do Rio de Janeiro Rio de Janeiro Brazil

The propagation of electromagnetic waves in the annular region of oil wells was studied. The present study aims to analyse the propagation attenuation along the well, as well as the input impedance determined by a source placed near the wellhead. A coaxial waveguide model was adopted with heterogeneous dielectrics and losses. First, a wave equation solution for the waveguide is presented, assuming a homogeneous medium with losses, by solving the equation in cylindrical coordinates using the vector potential technique. An uncertainty analysis model is then developed to model the heterogeneous characteristics of the medium. Monte Carlo simulations were performed with the created model using data gathered from the literature. The results of the simulations indicate that propagation in the transverse electromagnetic mode has the smallest attenuation and that for depths of up to 4000 m, there is an attenuation of less than 52 dB. Furthermore, the input impedance ranges from 10 Ω to 10 kΩ because of the uncertainties involved in the problem in question.

关键词： Electromagnetism Oil Well Wireless Telemetry Monte-Carlo Uncertainties Heterogeneous Media

Physical Exercise and Gene p53—A Mini Review

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Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis ×E. oleifera</i>

Journal of Cancer Therapy（癌症治疗） 2020年第8期

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Agricultural Sciences 2013年第11期4卷 1-11页

作者： Paula Cristina da Silva Angelo Douglas André Steinmacher ricardo Lopes Raimundo Nonato Vieira da cunha Miguel Pedro Guerra Embrapa Western Amazon Manaus Brazil UFSC Federal University of Santa Catarina—CCA Graduate Program in Plant Genetic Resources Physiology of Development and Plant Genetics Laboratory Florianópolis Brazil Brazilian Council for Technological Development—CNPq Brazil

Elaeis guineensis (African oil palm) and E. oleifera (American oil palm) are bred to attain high oil yields, disease resistances, and decelerated shoot elongation. We cultivated immature zygotic embryos from backcrossed and F1 interspecific progenies on media containing 110, 150, or 200 mg·l-1 2,4-diclorophenoxyacetic acid (2,4-D) to obtain embryogenic cultures. These were set to multiply on medium containing 8 mg·l-1 2,4-D or lower concentrations of auxins and finally we induced plantlets regeneration, from each zygotic embryo, independently, in order to have the clones organized according to their respective origins. Reductions in auxins induced cultures to revert from highly embryogenic into competent for embryogenesis and finally to organogenic degenerated callus lines. Histology and the expression of SOMATIC EMBRYOGENESIS RECEPTOR KINASE, DEHYDRIN, DEFENSIN, TRANSPOSASE, and LIPID TRANSFER PROTEIN were analyzed on four callus lines representative of morphological aspects consistently observed. The highest number of embryogenic cultures was obtained on 150 mg·l-1 2,4-D. Maturation and multiplication of somatic embryos through secondary embryogenesis occurred simultaneously on 8 mg·l-1 2,4-D. LIPID TRANSFER PROTEIN expression was detected in one of the embryogenic cultures and correlated with protoderm onset. Three six-week cycles on induction medium yielded 1.5 shoots above 6 cm per poly-embryogenic complex, which performed better than embryoids individualized mechanically. Rooting was observed for 77% and 82% of shoots from these two types of explants, respectively. Rooted plantlets ready for acclimatization were obtained nine months after shoot

关键词： Amazon Arecaceae Dendê Caiaué Gene Expression

Acellular Amniotic Membrane Patch Regeneration for Burn Injury: The Future for Wound Healing

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