检索结果-南通市图书馆

Enhancement of naked FIX minigene expression by chloroquine in mice

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第5期25卷 28-33页

作者： Hong-yan CHEN, Huan-zhang ZHU, Bin LU, Xuan XU, Ji-hua YAO, Qi SHEN, Jing-lun XUE1State Key Laboratory of Genetic Engineering, institute of Genetics,School of Life Sciences, fudan university, Shanghai 200433, China State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai 200433 China

AIM: To study the effect of chloroquine on the expression of human clotting factor IX (hFIX) in mice. METHODS:Hydrodynamics-based naked DNA plasmid administration was performed by tail vein injection of 10 g of pCMV-hFIX and chloroquine (0, 100, 200, and 500 mol/L) in 2.2 mL of Ringer solution within 6-7 s, the level andstability of hFIX expression, liver damage and toxicity were then examined. RESULTS: The maximum expression ofhFIX level was 4.41.8 mg/L at 8 h after injection, 9.71.6 mg/L at 24 h only existed in 200 mol/L chloroquine-treated animals, which is 3-4 fold higher than that of control (Pliver histological study showed the damage of liverwas not related to chloroquine (P>0.05). CONCLUSION: Chloroquine can enhance and sustain exogenous geneexpression in vivo without side effect under our experimental conditions.

关键词： chloroquine factor IX naked plasmids

ESTABLISHMENT OF AVIAN FEATHER PULP CULTURE METHOD AND STUDY ON THE CELL DIVISION

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

动物学研究 1987年第1期 25-26页

作者： Zhu Sutian Lu Qun | Xiang Wei(institute of Genetics, fudan university Shanghai)

It is difficult to obtain enough metaphase chrolmosomes for avian cytogene-tic analysis by conventional peripheral blood culture *** to now,the bone marrow technique is applied in a few of avian *** this te-chnique ca... 详细信息

关键词： Feather pulp Avian cytogenetics SCD technique The sex determination of birds

Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第9期36卷 589-596页

作者： Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, fudan university, Shanghai 200433, China 1The Veterinary institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2The Virus research institute of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China State Key Laboratory of Genetics Engineering Life Science School Fudan University The Veterinary institute of Chinese Academy of Agricultural Sciences

In this study, two DNA fragments encoding amino acid (141–160)-(21–40)-(141–160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138–160)-(21–40)-(138–160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine- IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

关键词： foot-and-mouth disease FMDV serotype O FMDV serotype A VP1 protein bivalent vaccine guinea pig

Effects of cryopreservation and phenylacetate on biological characters of adherent LAK cells from patients with hepatocellular carcinoma

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第2期8卷 233-236页

作者： Ning Zheng Sheng-Long Ye Rui-Xia Sun Yan Zhao Zhao-You Tang,liver cancer institute and Zhongshan Hospital,fudan university,Shanghai 200032,China liver cancer institute Zhongshan Hospital Fudan University Shanghai 200032 Chin

AIM: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects ofcryopreservation and phenylacetate (PA) on biologicalcharacters of A-LAK ***: A-LAK cells were obtained from peripheral bloodmononuclear cells (PBMCs) of the patients withhepatocellular caminoma (HCC) by using L-phenylalaninemethyl ester ( PME ) to deplete immunosuppressivermonocytes. Proliferative activity of SMMC7721 cell line aftertreatment with phenylacetate (PA) was observed. A-LAKcells were treated with the supernatant of SMMC7721 cellsthat had been pretreated with PA. The changes ofproliferation, cytotoxicity and phenotype of A-LAK cellswere investigated after ***: The expansion of A-LAK cells (96.79± 69.10 foldson Day 14 ) was significantly higher then that of non-adherent LAK (NA-LAK) cells (22.77 ± 13.20) as well asconventional LAK cells (4. 64 ± 0. 91 ). PA significantlysuppressed the growth of SMMC7721 cells, and the inhibitorratio was 46 %. rite supernatant of cultured tumor cellsintensively suppressed the proliferation and cytotoxicity ofA-LAK cells, but the suppressive effect of the supernatantwas previously decreased after treatment with *** in proliferation and cytotoxicity of A-LAK cellsimmediately after thawing of cryopreservation and recoveryafter reincubation with IL-2 were observed. The cytotoxicltyof thawed A-LAK cells on Day 5 was significantly higher thanthat of fresh A-LAK before freezing (54.8 ± 10.2 % vs 40.5 ±6. 4 %). No significant change in the percentage oflymphocyte subsets was identified in frozen A-LAK cells ascompared with that in the fresh control ***: A-LAK cells can he simply prepared by usingPME, and showed a synergistic anti-tumor effect with thecombination of PA. Cryopreseration can increase theimmunoactivities of A-LAK cells from the patients withhepatocellular caminoma .

关键词：肝细胞癌粘附淋巴因子活化杀伤细胞冷冻保存醋酸苯酯

E-钙黏蛋白及其相关蛋白α-连蛋白在小鼠胚胎着床过程中子宫内膜的表达

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

复旦学报（医学版） 2003年第1期30卷 41-43页

作者：朱瑾周剑萍肖立刘银坤张炜复旦大学附属妇产科医院上海200011 华东医院病理科上海200050 中山医院肝癌研究所上海200032

目的　E 钙黏蛋白及其相关蛋白α 连蛋白在小鼠胚胎着床过程中的蛋白定位及表达的动态变化。方法　小鼠雌雄合笼后建立小鼠早孕模型 ,用免疫组化的方法 ,检测α 连蛋白在小鼠胚胎着床过程中子宫内膜表达的定位 ;用免疫印迹的方法定量分... 详细信息

目的　E 钙黏蛋白及其相关蛋白α 连蛋白在小鼠胚胎着床过程中的蛋白定位及表达的动态变化。方法　小鼠雌雄合笼后建立小鼠早孕模型 ,用免疫组化的方法 ,检测α 连蛋白在小鼠胚胎着床过程中子宫内膜表达的定位 ;用免疫印迹的方法定量分析E 钙黏蛋白及其相关蛋白α 连蛋白在小鼠胚胎着床过程中表达的动态变化。结果　定性结果表明 ,α 连蛋白在小鼠妊娠d1～d5子宫内膜的表达主要定位在子宫内膜腺上皮的细胞膜 ,随着妊娠的继续 ,从妊娠d6开始 ,其表达逐渐转移到基质的蜕膜细胞膜表面。半定量结果表明 ,E 钙黏蛋白和α 连蛋白在小鼠胚胎着床过程中的峰值同时出现在小鼠妊娠的第 4天 ,即胚胎着床前几小时它们的共同表达。结论　E 钙黏蛋白和α

关键词： E-钙黏蛋白 α-连蛋白免疫组织化学小鼠胚胎着床子宫内膜

Corn Umbilicus Tissue-Based Membrane Biosensor for Pyruvate

在线全文

学校读者我要写书评

暂无评论

Chemical research in Chinese Universities 1992年第1期8卷 37-40页

作者： Fang Yao , Cai Ronghui and Zhang Yonghua (Changchun institute of Applied Chemistry, Academia Sinica, Changchun)Deng Jiaqi (Department of Chemistry, fudan university, Shanghai)

Pyruvate, a final metabolite of glycogen, is widely distributed over many kinds of tissue fluids of human bodys. The determination for its contents is of clinical importance in gynaecology. Titrimetry, colorimetry and chromatography are common used methods. In recent years, enzymatic mthod has proved to be one of the most important determination techniques for its simplicity and fastness. However, in some cases, the use of purified enzymes as biocatalysts yields systems with a short useful lifetime owing, to enzyme instability A tiss

关键词： Pyruvate Decarboxylase Tissue-based membrane electrode Biosensor.

Microwave digestion method in environmental analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 1996年第4期8卷 407-413页

作者： Zhang Yue (Wadsworth Centre ,New York State Department of Health School of Public Health,State university of New York at Albany.USA) Yu Yong, Hou Huiqi,Li Changlin(Environmental Science institute,fudan university,Shanghai 200433, China) New York State Department of Health School of Public Health State University of New York Albany United States Environmental Science institute Fudan University Shanghai 200433 China

This paper introduced the history,equipment and mechanism of the microwave digestion method. This paper studied the optimization parameter of digestion conditions, such as acid and acid combination, digestion time and applied microwave power,particle size,and other *** analytical performance in environmental research and the modification of the microwave digestion method were also discussed.

关键词： microwave digestion environmental analysis optimization parameter.

A measure of semantic similarity between gene ontology terms based on semantic pathway covering

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2006年第7期16卷 721-726页

作者： LI Rong, CAO Shunliang, LI Yuanyuan, TAN Hao, ZHU Yangyong, ZHONG Yang and LI Yixue ( Department of Computing and Information Technology, fudan university, Shanghai 200433, China Shanghai Center for Bioin-formation Technology, Shanghai 200235, China School of Life Sciences, fudan university, Shanghai 200433, China Bioinfor-rnation Center of Shanghai institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) Shanghai Ctr Bioinformat Technol Shanghai 200235 Peoples R China fudan Univ Dept Comp & Informat Technol Shanghai 200433 Peoples R China fudan Univ Sch Life Sci Shanghai 200433 Peoples R China Chinese Acad Sci Shanghai Inst Biol Sci Bioinformat Ctr Shanghai 200031 Peoples R China

Semantic similarity between Gene Ontology (GO) terms is critical in resolving semantic heterogeneousness when integrating heterogeneous biological databases. Traditionally, distance based and information content based measures are two major methods. In this paper, a new method based on semantic pathway covering is proposed and an algorithm, COMBINE algorithm, is presented, which considers information contents of two given nodes and those of all nodes included in the two nodes' pathways. Experiments show that COMBINE algorithm obtains the highest correlation index compared with those distance based and information content based algorithms.

关键词： Gene Ontology semantic similarity measure information content.

Knowledge improvement of guidelines for COPD and asthma:an educational intervention among physicians in Shanghai,China

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Knowledge improvement of guidelines for COPD and asthma:an e...