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纸上反相分配色谱中铀的协同萃取效应

化学学报 1982年第8期40卷 747-751页

作者：秦启宗王高栋上海复旦大学中国科学院上海原子核研究所

溶剂萃取中采用混合萃取剂所产生的协同效应,已获得了广泛的研究。特别是以螯合萃取剂与中性萃取剂组成的协同萃取体系,更引起人们的重视。 Cvjeticanin 将纸上反相分配色谱用于协萃效应的研究。选用螯合萃取剂噻吩甲酰三氟丙酮(TTA)与... 详细信息

溶剂萃取中采用混合萃取剂所产生的协同效应,已获得了广泛的研究。特别是以螯合萃取剂与中性萃取剂组成的协同萃取体系,更引起人们的重视。 Cvjeticanin 将纸上反相分配色谱用于协萃效应的研究。选用螯合萃取剂噻吩甲酰三氟丙酮(TTA)与中性磷类萃取剂磷酸三丁酯(TBP)、三辛基氧化膦(TOPO)或磷酸三辛酯(TOP)组成的混合萃取剂作固定相,研究了对铀、钍和稀土元素的协萃效应,并探讨了有关的协萃机理。本文以8-羟基喹啉(HOx)或中性磷类萃取剂TBP、甲基膦酯二异戊酯(DAMP)、丁基膦酸二丁酯(DBBP)以及由它们所组成的两组分混合萃取剂作固

关键词： TBP HO pH 固定相相对体积展开剂萃取剂分析试剂

NUMERICAL SIMULATION OF HEMODYNAMICS IN THE HOST BLOOD VESSEL AND MICROVASCULAR NETWORK GENERATED FROM TUMOR-INDUCED ANGIOGENESIS

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Journal of Hydrodynamics 2006年第6期18卷 727-735页

作者： ZHAO Gai-ping WU Jie XU Shi-xiong COLLINS M.W. JIANG Yu-ping WANG Jian Department of Mechanics and Engineering Science Fudan University Shanghai 200433 China Brunel institute for Bioengineering School of Engineering and Design Brunel University Uxbridge MiddlesexUB8 3PH UK Department of Neurology Huashan Hospital Fudan University Shanghai 200040 China

Numerical simulation of hemodynamics under the combined effects of both the host blood vessel and the microvascular network, which is based on a 2-D tumor inside and outside vascular network generated from a discrete mathematical model of tumor-induced angiogenesis, is performed systemically. And a ＂microvascular network-- transport across microvascular network--flow in interstitium＂ model is developed to study the flow in solid tumor. Simulations are carried out to examine the effects of the variations of the inlet Reynolds number in the host blood vessel, the hydraulic conductivity of the microvascular wall, and interstitial hydraulic conductivity coefficient on the fluid flow in tumor microcirculation. The results are consistent with data obtained in terms of physiology. These results may provide some theoretical references and the bases for further clinical experimental research.

关键词： solid tumors host blood vessel microvascular network hemodynamics numerical simulation

Induction of liver cytochrome P450 1A2 expression by flutamide in rats

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Acta Pharmacologica Sinica 2005年第11期26卷 1382-1386页

作者： Hai-xue WANG, Xiao LIU, Chang-jiang XU, Xiao-chao MA~3, Jian-er LONG~4, Duan LI~(4,5) ~2 Department of Pharmacology, School of Pharmacy, fudan university, Shanghai 200032, China ~3 State Key Laboratory of Drug research, Shanghai institute of Biological Sciences, Shanghai institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China ~4 institute of Molecular Virus, School of Basic Medical Science, fudan university, Shanghai 200031, China DepartmentofPharmacology SchoolofPharmacyFudanUniversityShanghai200032China

Aim: To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Methods: Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. liver CYP 1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYPl A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYPl A2 substrate. Results: CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYPl A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity. Conclusion: Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.

关键词： flutamide cytochrome P450 1A2 induction

Transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site

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Cell research 2006年第9期16卷 780-796页

作者： Jing De Zhu~(1,2) Qi Fei~(1,*) Peng Wang~(1,*) Fei Lan~(1,*) Da Qin Mao~(1,*) Hong Yu Zhang~1 Xue Biao Yao~3 1 Laboratory of cancer Eplgenetics and Gene Therapy,The State-Key Laboratory for Oncogenes and Related Genes,Shanghai cancer institute,Shanghai Jiaotong university,LN 2200/25,Xietu Road,Shanghai 200032,China 2 School of Life Sciences,Zhejiang Science and Technology university,Xiasha university District,Hangzhou,Zhejiang 310018,China, 3 Laboratory of Cellular Dynamics,university of Science and Technology of China,Hefei 230027,China 2School of Life Sciences Zhejiang Science and Technology University Xiasha University District Hangzhou Zhejiang 310018 China 1Laboratory of cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 3Laboratory of Cellular Dynamics University of Science and Technology of China Hefei 230027 China

The hepatocellular carcinoma suppressor 1 (HCCS1) gene was identified by both positional cloning from a predominantregion of loss of heterozygosity (17p13.3) in liver cancer and by functional screening for genes affecting cell proliferationin large-scale transfection *** overexpression results in inhibition of cell proliferation in cell culture and tumorgrowth in nude *** understand its transcription regulation,the promoter architecture has been dissected in *** start of transcription was mapped by primer extension to a C residue,177 nucleotides upstream of the ATG *** assessing the promoter activity of a set of linker-scanning mutants of the minimal promoter (-60 to +148 region) ina transient transfection assay,we found that the+1 to+40 region is critical to HCCSI gene transcription,containingbinding sites for transcription factors NF-κB(-21 to+7 and+40 to+26),p53 (+29 to+9) and ETS (+4 to+20 and+23to+39).Biochemical and molecular analyses revealed that the ETS transcription factors ETS-2 and Elf-1 bind to thetwo ETS sites in situ and contribute significantly to the transcriptionally active state of the HCCS1 gene,while NF-κB,p53 and two other members of the ETS family (ETS-1 and NERF2) appear to play little *** observations provideinsight into the mechanistic aspects of HCCS1 transcription regulation.

关键词： HCCS1 gene transcription regulation ETS p53 NF-κB

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中国东、中、西三大地带人口迁移特征分析

市场与人口分析 2004年第5期10卷 13-22页

作者：魏星王桂新复旦大学人口研究所上海200433

中国东部、中部、西部三个地带在自然环境条件、历史开发程度、经济发展水平等方面存在地带间显著差异和地带内相对同质的鲜明特点。这使跨地带人口迁移成为省际人口迁移的主要模式和重要组成部分。从东、中、西三大地带角度考察跨地带... 详细信息

中国东部、中部、西部三个地带在自然环境条件、历史开发程度、经济发展水平等方面存在地带间显著差异和地带内相对同质的鲜明特点。这使跨地带人口迁移成为省际人口迁移的主要模式和重要组成部分。从东、中、西三大地带角度考察跨地带人口迁移的数量与流向及分布、移民基本属性特征、在业状况及迁移原因等方面的特征。

关键词：中国东部地区中部地区西部地区人口迁移特征移民教育就业问题

Losartan inhibited expression of matrix metalloproteinases in rat atherosclerotic lesions and angiotensin Ⅱ-stimulated macrophages

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Acta Pharmacologica Sinica 2004年第11期 42-48页

作者： Chun LIANG, Zong-gui WU, Jian DING, Jian-fei JIANG, Gao-zhong HUANG, Rong-zeng DU, Jun-bo GEDepartment of Cardiology, Changzheng Hospital, Second Military Medical university, Shanghai 200003, China Shanghai institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China Shanghai institute of Cardiovascular Diseases, Zhongshan Hospital, fudan university, Shanghai 200032, China

AIM: To explore whether the angiotensin Ⅱ (Ang Ⅱ) receptor 1 (AT1) antagonist, losartan could reduce activity and expression of matrix metalloproteinases (MMPs) in rat atherosclerotic plaques. METHODS: Male Wistar-Kyoto rats were ip injected a single dose of vitamin D3 600 kU·kg-1·month-1 and fed an atherogenic diet for 4 months to induce experimental atheroma. Then either placebo or losartan 50 mg·kg-1·d-1 was administered in rats for another 2 months. In vitro, the effect of losartan 0.1-10μmol/L on the expression of MMP-2 and MMP-9 was investigated in Ang II-stimulated rat peritoneal macrophages. The expression and activity of MMP-2 and MMP-9 were monitored by Western blot, RT-PCR, and SDS-PAGE zymography analysis. RESULTS: High levels of MMP-2 and MMP-9 were expressed in rat atherosclerotic lesions. Losartan significantly reduced the activity and expression of MMP-2 and MMP-9 compared with the placebo group (MMP-2, 5861±539 vs 8991±965, P<0.05; MMP-9, 10527±1002 vs 14623±2462, P<0.01). In

关键词： losartan metalloproteinases angiotensin Ⅱ atherosclerosis peritoneal macrophages reverse tran-scriptase polymerase chain reaction Western blotting

The tumor-selective over-expression of the human Hsp 70 gene is attributed to the aberrant controls at both initiation and elongation levels of transcription

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Cell research 2003年第2期13卷 93-109页

作者： LING CAI, JING DE ZHU,The State-key Laboratory for Oncogenes and Related Genes, Shanghai cancer institute, LN 25/2200, Xie-tu Road, Shanghai 200032, China cancer Genetics and Gene Therapy The State-key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao Tong University LN 25/2200 Xie-tu Road Shanghai 200032 China

The tumor selective over-expression of the human Hsp70 gene has been well documented in human tumors, linked to the poor prognosis, being refractory to chemo- and radio-therapies as well as the advanced stage of tumorous lesions in particular. However, both the nature and details of aberrations in the control of the Hsp70 expression in tumor remain enigmatic. By comparing various upstream segments of the Hsp70 gene for each’s ability to drive the luciferase reporter genes in the context of the tumor cell lines varying in their p53 status and an immortal normal liver cell line, we demonstrated in a great detail the defects in the control mechanisms at the both initiation and elongation levels of transcription being instrumental to the tumor selective profile of its expression. Our data should not only offer new insights into our understanding of the tumor specific over-expression of the human Hsp70 gene, but also paved the way for the rational utilization of the tumor selective mechanism with the Hsp70 a

关键词： Hsp 70 Tumor transcription elongation 5’ UTR. Over-expression.

理念与技术并重提高食管胃结合部腺癌综合治疗水平

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中国实用外科杂志 2012年第4期32卷 261-263页

作者：秦新裕刘凤林复旦大学附属中山医院普外科复旦大学普通外科研究所上海200032

目前,食管胃结合部腺癌(AEG)作为一类不同于食管癌和胃癌的独立疾病的临床观点已为多数学者所接受。然而,有关AEG根治术的手术径路、切除范围、淋巴结清扫范围等问题仍存在争议。外科医师应重视临床研究结果,同时提高手术技巧。在循证... 详细信息

目前,食管胃结合部腺癌(AEG)作为一类不同于食管癌和胃癌的独立疾病的临床观点已为多数学者所接受。然而,有关AEG根治术的手术径路、切除范围、淋巴结清扫范围等问题仍存在争议。外科医师应重视临床研究结果,同时提高手术技巧。在循证医学的指导下,应综合病人全身情况、AEG类型、cTNM分期等因素,开展多学科团队治疗模式,制定合理的个体化综合治疗方案。

关键词：食管胃结合部腺癌淋巴结清扫

Cardiac stem cell therapy research in China

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Journal of Geriatric Cardiology 2006年第3期3卷 184-186页

作者： Junbo GE Center of Stem Cell and Tissue Engineering Shanghai Institute of Cardiovascular Disease Zhongshan HospitalFudan University Shanghai 200032 China

For more than two decades, the morbidity and mortality of coronary artery disease (CAD) has been increasing rapidly in China. Despite tremendous advances in treatment strategies of CAD, heart failure after acute myocardial infarction (AMI) continues to be one of the greatest medical challenges throughout the world. In 1994, Soonpaa and colleagues first reported the possibility of cardiomyocytes implantation and suggested that intracardiac cell grafting might provide a useful approach for myocardial repair.1 Cell implantation has become a novel therapeutic option for ischemic cardiac injury and heart failure.

关键词： stem cell Cardiac stem cell therapy research in China