Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection
Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection作者机构:Department of Microbiology Medical Center of Fudan University Shanghai 200032 China. zmswq@***
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2001年第7卷第3期
页 面:335-339页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题:Carcinoma, Hepatocellular Cell Adhesion Cell Movement Gelatinase A Gelatinase B Gene Expression Regulation, Neoplastic Genes, ras Humans In Vitro Liver Neoplasms Phenotype Predictive Value of Tests Receptor, Epidermal Growth Factor Transfection
摘 要:AIM: To demonstrate the relationship between H-ras oncogene and hepatocellular carcinoma (HCC) metastasis. METHODS: Activated H-ras oncogene was transfected into SMMC 7721, a cell line derived from human HCC, by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expression of collagenase IV(c IV ase) and epidermal growth factor receptor (EGFR). RESULTS: The abilities of H-ras-transfected cell clones in adhesion to laminin (LN) or fibronectin (FN), migration, c IV ase secretion increased markedly, and the expression of EGFR elevated moderately. More importantly, these alterations were consistent positively with the expression of p21, the protein product of H-ras oncogene. CONCLUSION: H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro to raise its metastatic potential.