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Integration of multiple flexible electrodes for realtime detection of barrier formation with spatial resolution in a gut-on-chip system

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Microsystems & Nanoengineering 2024年第1期10卷 105-114页

作者： Mara lucchetti Gabriel Werr Sofia Johansson laurent Barbe léa Grandmougin Paul Wilmes maria Tenje luxembourg Centre for Systems Biomedicine(lCSB) University of LuxembourgEsch-sur-Alzette L-4362Luxembourg Division of Biomedical Engineering Department of Materials Science and EngineeringScience for Life LaboratoryUppsala University75121 UppsalaSweden Department of life Sciences and Medicine Faculty of ScienceTechnology and MedicineUniversity of LuxembourgEsch-sur-Alzette L-4362Luxembourg

In healthy individuals,the intestinal epithelium forms a tight barrier to prevent gut bacteria from reaching blood *** study the effect of probiotics,dietary compounds and drugs on gut barrier formation and disruption,human gut epithelial and bacterial cells can be cocultured in an in vitro model called the human microbial crosstalk(HuMiX)gut-on-a-chip ***,we present the design,fabrication and integration of thin-film electrodes into the HuMiX platform to measure transepithelial electrical resistance(TEER)as a direct readout on barrier tightness in *** various aspects of the HuMiX platform have already been set in their design,such as multiple compressible layers,uneven surfaces and nontransparent materials,a novel fabrication method was developed whereby thin-film metal electrodes were first deposited on flexible substrates and sequentially integrated with the HuMiX system via a transfer-tape ***,to measure localized TEER along the cell culture chamber,we integrated multiple electrodes that were connected to an impedance analyzer via a *** further developed a dynamic normalization method because the active measurement area depends on the measured TEER *** fabrication process and system setup can be applicable to other barrier-on-chip *** a proof-of-concept,we measured the barrier formation of a cancerous Caco-2 cell line in real-time,which was mapped at four spatially separated positions along the HuMiX culture area.

关键词： barrier system multiple

Effect of low concentration electrolytes on the formation and corrosion resistance of PEO coatings on AM50 magnesium alloy

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Journal of Magnesium and Alloys 2024年第4期12卷 1386-1405页

作者： Peng Xie Carsten Blawert maria Serdechnova Natalia Konchakova Tatsiana Shulha Ting Wu Mikhail l.Zheludkevich Institute of Surface Science Helmholtz-Zentrum HereonMax-Planck Strasse 121502 GeesthachtGermany Institute for Materials Science Faculty of EngineeringKiel UniversityKaiserstrasse 224143 KielGermany

In this paper,the formation process,morphology,and electrochemical performance of PEO coatings on AM50 magnesium alloy prepared in low concentration phosphate,aluminate,and phosphate-aluminate electrolytes were systematically *** results show that the coatings prepared from the phosphate electrolytes have a higher thickness and better corrosion resistance properties compared to the other *** coatings prepared from low concentration phosphate-aluminate mixed electrolytes have slightly thinner thickness,a similar coating structure and an order of magnitude lower value of electrochemical impedance compared with phosphate electrolyte *** Coatings prepared from low concentration aluminate electrolytes have the lowest thickness and the worst corrosion resistance properties which gets close to corrosion behavior of the bare AM50 under the same test *** application,coatings prepared from single low concentration phosphate electrolytes and low concentration phosphate-aluminate electrolytes have greater potential than single low concentration aluminate ***,reducing the electrolyte concentrations of coating forming ions too much has negative influence on the coating growth rate.

关键词： Plasma electrolytic oxidation low concentration electrolytes Corrosion resistance AM50 magnesium alloy

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lDH sealing for PEO coated friction stir welded AZ31/AA5754 materials

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Nano Materials Science 2024年第4期6卷 428-442页

作者： Tatsiana Shulha maria Serdechnova Ting Wu Thymoty Naacke Gert Wiesea Carsten Blawert Mikhail l.Zheludkevich Institute of Surface Science Helmholtz-Zentrum Hereon Faculty of Engineering CAU Kiel University

The need to combine various metals in light-weight constructions requires the development of coatings that prevent galvanic corrosion. layered double hydroxides(lDHs) can be an example of such coatings, which were previously successfully obtained in situ on individual materials. In addition, the possibility of lDH growth(including lDH growth in the presence of chelating agents) on the surface of plasma electrolytic oxidation(PEO)-coated metals was previously shown. This PEO + lDH combination could improve both corrosion and mechanical characteristics of the system. The possibility of lDHs formation in situ on the surface of PEO-coated friction stir welded(FSW) magnesium-aluminum materials(AZ31/AA5754 system was selected as a model one) was demonstrated in the presence of 1,3-diamino-2-hydroxypropane-N,N,N',N'-tetraacetic acid(DHPTA) as a chelating agent, which was selected based on analysis of respective metal-ligand compounds stability. lDHs growth was achieved under ambient pressure without addition of carbonates in the electrolyte. The effectiveness of the resulting coating is shown both for corrosion resistance and hardness.

关键词： layered double hydroxides (lDHs) Plasma electrolytic oxidation (PEO) Chelating agent Welded magnesium-aluminum materials Functional coatings

Prevalence of Helicobacter pylori infection among patients with esophageal carcinoma

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World Journal of Gastroenterology 2024年第29期30卷 3479-3487页

作者： Miriam lópez-Gómez maria Morales Rebeca Fuerte Marta Muñoz Pedro-David Delgado-lópez Jorge Francisco Gómez-Cerezo Enrique Casado Department of Medical Oncology Precision Oncology LaboratoryInfanta Sofía University HospitalSan Sebastián de los Reyes 28231MadridSpain Department of Medical Oncology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain Department of Internal Medicine Infanta Sofía University HospitalSan Sebastián de los Reyes 28703MadridSpain Department of Pathology Infanta Sofía University HospitalSan Sebastián de los Reyes 28702Spain Department of Neurosurgery Burgos University HospitalBurgos 09006Spain Department of Internal Medicine Infanta Sofía University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain Department of Medical Oncology Infanta Sofia University Hospital and Henares University Hospital Foundation for Biomedical Research and InnovationSan Sebastian de los Reyes 28702MadridSpain

BACKGROUND Helicobacter pylori(***)is a widespread microorganism related to gastric adenocarcinoma(AC).In contrast,it has been reported that an inverse association exists between *** infection and esophageal *** mechanisms underlying this supposedly protective effect remain *** To determine the prevalence of *** infection in esophageal carcinoma patients,we performed a retrospective observational study of esophageal tumors diagnosed in our *** We retrospectively reviewed the prevalence of *** infection in a cohort of patients diagnosed with esophageal *** or previous proton pump inhibitor(PPI)usage was also *** A total of 89 patients with esophageal carcinoma(69 males,77.5%),with a mean age of 66 years(range,26-93 years)were *** was the most frequent pathological variant(n=47,52.8%),followed by squamous cell carcinoma(n=37,41.6%).Fourteen ACs(29.8%)originated in the gastroesophageal junction and 33(70.2%)in the esophageal ***,54 patients(60.7%)presented at stages III and *** *** infection occurred only in 4 patients(4.5%),3 with AC(6.3%of all ACs)and 1 with squamous cell carcinoma(2.7%of all squamous cell tumors).All patients with previous *** infection had stage *** one patient had received prior *** eradication therapy,whereas 86(96.6%)had received previous or concomitant PPI *** In our cohort of patients,and after histologic evaluation of paraffin-embedded primary tumors,we found a very low prevalence of previous *** *** also reviewed the medical history of the patients,concluding that the majority had received or were on PPI *** minimal prevalence of *** infection found in this cohort of patients with esophageal carcinoma suggests a protective role.

关键词： Helicobacter pylori Eradication Esophageal tumor Dysbiosis Proton pump inhibitors Carcinogenesis Microbiota Incidence

Pesticides in a temperate coastal lagoon in southwest Europe (Ria de Aveiro, Portugal) – Risk assessment and acute assays with Artemia and Daphnia

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Emerging Contaminants 2024年第2期10卷 40-56页

作者： maria João Rocha Eduardo Rocha laboratory of Histology and Embryology Department of MicroscopyICBAS-School of Medicine and Biomedical SciencesUniversity of Porto(U.Porto)Portugal Team of Animal Morphology and Toxicology Interdisciplinary Centre of Marine and Environmental Research(CIIMAR/CIMAR)U.PortoPortugal

The Ria de Aveiro lagoon has been considered one of the most important Southern European long-term monitoring sites(lTER-site)concerning climate change and anthropogenic ***,studies concerning pesticide contamination herein are few and mostly ***,56 pesticides dissolved in the aqueous phase(DAP)and suspended particulate matter(SPM)were examined to fill the knowledge gap,considering the hydrophobic nature of most evaluated *** samples were taken seasonally from eight sites,and both DAP and SPM fractions were analysed by gas-chromatography-mass *** annual average concentrations of fungicides,herbicides and insecticides were≈57 ng/l,≈409 ng/l and≈1058 ng/l in DAP and≈7μg/g,≈28μg/g and≈67μg/g in *** pesticides and the excessive phosphate concentrations suggest that the lagoon's water quality may be hazardous to biota and *** were identified as the most threatened trophic level using deterministic risk assessment,with chlorpyriphos and chlorfenvinphos being the primary *** experiments with Artemia and Daphnia supported the suppositions.

关键词： Coastal lagoon Invertebrate bioassays Risk evaluation Waterborne pesticides

Non-pancreatic hyperlipasemia:A puzzling clinical entity

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World Journal of Gastroenterology 2024年第19期30卷 2538-2552页

作者： Krisztina Eszter Feher David Tornai Zsuzsanna Vitalis laszlo Davida Nora Sipeki maria Papp Division of Gastroenterology Department of Internal MedicineFaculty of MedicineUniversity of DebrecenDebrecen H-4032Hajdu-BiharHungary Kalman laki Doctoral School of Biomedical and Clinical Sciences Faculty of MedicineUniversity of DebrecenDebrecen H-4032Hungary

BACKGROUND Increased lipase level is a serological hallmark of the diagnosis of acute pancreatitis(AP)but can be detected in various other diseases associated with lipase leakage due to inflammation of organs surrounding the pancreas or reduced renal clearance and/or hepatic *** non-pancreatic hyperlipasemia(NPHl)is puzzling for attending physicians during the diagnostic procedure for *** would be clinically beneficial to identify the clinical and laboratory variables that hinder the accuracy of lipase diagnosis with the aim of improve it.A more precise description of the NPHl condition could potentially provide prognostic factors for adverse outcomes which is currently *** To perform a detailed clinical and laboratory characterization of NPHl in a large prospective patient cohort with an assessment of parameters determining disease *** A Hungarian patient cohort with serum lipase levels at least three times higher than the upper limit of normal(UlN)was prospectively evaluated over 31 *** were identified using daily electronic laboratory reports developed to support an ongoing observational,multicenter,prospective cohort study called the EASY trial(ISRCTN10525246)to establish a simple,easy,and accurate clinical scoring system for early prognostication of *** of NPHl was established based on≥3×UlN serum lipase level in the absence of abdominal pain or abdominal imaging results characteristic of *** A total of 808 patients[male,n=420(52%);median age(IQR):65(51-75)years]were diagnosed with≥3×UlN serum lipase levels.A total of 392 patients had AP,whereas 401 had NPHl with more than 20 different *** and acute kidney injury(AKI)were the most prevalent etiologies of NPHl(27.7%and 33.2%,respectively).The best discriminative cut-off value for lipase was≥666 U/l(sensitivity,71.4%;specificity,88.8%).The presence of AKI or sepsis negatively affected the diagnostic performance of *** w

关键词： Non-pancreatic hyperlipasemia Acute pancreatitis Glycoprotein 2 Acute kidney injury Sepsis Mortality

Ion-conducting Membranes Based on Bacterial Cellulose Nanofibers Modified by Poly(sodium acrylate-co-2-acrylamido-2-methylpropanesulfonic acid)

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Chinese Journal of Polymer Science 2024年第3期42卷 333-343,I0007页

作者： Elizaveta V.Batishcheva Nikolay N.Smirnov Natalya V.Bobrova maria P.Sokolova Michael A.Smirnov Institute of Macromolecular Compounds Russian Academy of SciencesBolshoy pr.31Saint Petersburg 199004Russia

Green method for preparation of ion-conducting membranes(ICM) based on bacterial cellulose nanofibers(CNF) modified by a copolymer of sodium acrylate and 2-acrylamido-2-methylpropanesulfonic acid was elaborated. FTIR and NMR data confirmed grafting of polyacrylate onto cellulose surface. Formation of porous structure of the ICM was controlled by SEM and AFM. The maximal ionic conductivity of the membranes reaches 1.5 and 3.1 mS·cm^(-1)(60 ℃ and 98% relative humidity) when they are saturated with water or H_2SO_4(1 mol·l^(-1)) electrolyte,respectively. Prepared ICM was tested as a separator in a symmetrical supercapacitor with electrodes based on polyaniline hydrogel. The assembled cell demonstrate ability to operate at high current density up to 100 A·g^(-1) maintaining specific capacitance 165 F·g^(-1). Maximal specific capacitance of 289 F·g^(-1) was achieved at current density 1 A·g^(-1). Retaining of 90% of initial capacitance after 10000 of charge-discharge cycles proves high electrochemical stability of prepared ICM.

关键词： Bacterial cellulose Ion conductivity Porous membrane Supercapacitor

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liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

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Photonics Research 2024年第7期12卷 1564-1573页

作者： SANDRO C.OlIVEIRA maria S.SOARES BÁRBARA V.GONÇAlVES ANDREIA C.M.RODRIGUES AMADEU M.V.M.SOARES RITA G.SOBRAl NUNO F.SANTOS JAN NEDOMA PEDRO l.AlMEIDA CARlOS MARQUES CICECO-Aveiro Institute of Materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3N Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate laboratory i4HB-Institute for Health and Bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAM-Centre for Environmental and Marine Studies and Department of Biology University of Aveiro3810-193 AveiroPortugal Department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3N-CENIMAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal Department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/ml to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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Screen-printed graphite electrode on polyvinyl chloride and parchment strips integrated with genetic programming for in situ nitrate sensing of aquaponic pond water

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Information Processing in Agriculture 2024年第2期11卷 187-201页

作者： Ronnie ConcepcionⅡ Bernardo Duarte maria Gemel Palconit Jonah Jahara BaunArgel Bandala Ryan Rhay Vicerra Elmer Dadios Department of Manufacturing Engineering and Management De La Salle UniversityManila 1004 Philippines MARE-Marine and Environmental Sciences Centre.&ARNET-Aquatic Research Infrastructure Network Associated laboratory Faculdade de Ciencias da Universidade de LisboaCampo Grande1749-016 LisboaPortugal Departamento de Biologia Vegetal da Faculdade de Ciencias da Universidade de lisboa Campo Grande 1749-016 LisboaPortugal Department of Electronics and Computer Engineering De La Salle UniversityManila 1004 Philppines

Nitrate is the primary water-soluble macronutrient essential for plant growth that is converted from excess fish feeds,fish effluents,and degrading biomaterials on the aquaponic pond floor,and when aquacultural malpractices occur,large amounts of it retain in the water system causing increase rate in eutrophication and toxifies fish and aquaculture *** nitrate sensor prototypes still require performing the additional steps of water sample deionization and dilution and were constructed with expensive *** response to the challenge of sensor enhancement and aquaponic water quality monitoring,this study developed sensitive,repeatable,and reproducible screen-printed graphite electrodes on polyvinyl chloride and parchment paper substrates with silver as electrode material and 60:40 graphite powder:nail polish formulated conductive ink for electrical traces,integrated with 9-gene genetic expression model as a function of peak anodic current and electrochemical test time for nitrate concentration prediction that is embedded into low-power Arduino ESP32 for in situ nitrate sensing in aquaponic pond *** SPE electrical traces were designed on the two types of *** electron microscopy with energy dispersive X-ray confirmed the electrode surface *** cyclic voltammetry using 10 to 100 mg/l KNO3 and water from three-depth regions of the actual pond established the electrochemical test time(10.5 s)and electrode potential(0.135 V)protocol necessary to produce peak current that corresponds to the strength of nitrate ions during *** findings from in situ testing revealed that the proposed sensors have strong linear predictions(R2=0.968 MSE=1.659 for nSPEv and R2=0.966 MSE=4.697 for nSPEp)in the range of 10 to 100 mg/l and best detection limit of 3.15μg/l,which are comparable to other sensors of more complex *** developed three-electrode electrochemical nitrate sensor confirms that it is reliable for bot

关键词： Aquaponic water quality Electrochemical technology Genetic programming Graphite electrode Nitrate sensor Precision agriculture Printed electronics Scanning electron microscopy Screen-printed electrode Voltammetry