Integration of multiple flexible electrodes for realtime detection of barrier formation with spatial resolution in a gut-on-chip system
作者机构：Luxembourg Centre for Systems Biomedicine(LCSB)University of LuxembourgEsch-sur-Alzette L-4362Luxembourg Division of Biomedical EngineeringDepartment of Materials Science and EngineeringScience for Life LaboratoryUppsala University75121 UppsalaSweden Department of Life Sciences and MedicineFaculty of ScienceTechnology and MedicineUniversity of LuxembourgEsch-sur-Alzette L-4362Luxembourg
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2024年第10卷第1期
页 面：105-114页
核心收录：
学科分类：080903[工学-微电子学与固体电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0804[工学-仪器科学与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学]
基 金：funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie(grant agreement No.812954) the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program(grant agreement No.757444 and grant agreement No.863664) funded by the Swedish Research Council(2019-00207)as a national research infrastructure funding provided by Uppsala University
摘 要：In healthy individuals,the intestinal epithelium forms a tight barrier to prevent gut bacteria from reaching blood *** study the effect of probiotics,dietary compounds and drugs on gut barrier formation and disruption,human gut epithelial and bacterial cells can be cocultured in an in vitro model called the human microbial crosstalk(HuMiX)gut-on-a-chip ***,we present the design,fabrication and integration of thin-film electrodes into the HuMiX platform to measure transepithelial electrical resistance(TEER)as a direct readout on barrier tightness in *** various aspects of the HuMiX platform have already been set in their design,such as multiple compressible layers,uneven surfaces and nontransparent materials,a novel fabrication method was developed whereby thin-film metal electrodes were first deposited on flexible substrates and sequentially integrated with the HuMiX system via a transfer-tape ***,to measure localized TEER along the cell culture chamber,we integrated multiple electrodes that were connected to an impedance analyzer via a *** further developed a dynamic normalization method because the active measurement area depends on the measured TEER *** fabrication process and system setup can be applicable to other barrier-on-chip *** a proof-of-concept,we measured the barrier formation of a cancerous Caco-2 cell line in real-time,which was mapped at four spatially separated positions along the HuMiX culture area.
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