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Molecular Characterization of CMS Lines and Standardization of Hybrid Seed Production Technique in Chilli (Capsicum annuum L.)

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Journal of Agricultural Science and Technology(B) 2016年第4期6卷 238-243页

作者： Bhakchand Vitthal Tembhurne Kisan Babu Naveenkumar Gurumurthi Vishwanath Biradar Belbadevi Biradar ramappa lokesha Department o f Genetics and Plant Breeding College o f Agriculture University o f Agricultural Sciences Raichur 584101 Karnataka India Department ofMolecular Biology and Agricultural Biotechnology U niversityof Agricultural Sciences Raichur 584101 Karnataka India Department o f Agronomy University o f Agricultural Sciences Raichur 584101 Karnataka India

The study has been initiated with an aim to utilize cytoplasmic male sterile (CMS) associated gene fragment marker to understand the marker flow in segregating population and nature of dominance of the marker. And further it was aimed to understand the best pollination time for the maximum fruit set and to economise the chilli hybrid seed production based on CMS lines. Hence, two CMS based high yielding hybrids, which were found to be much more potential than that of the non CMS based hybrids, have been identified. The CMS gene was dissected from one of the high yielding hybrid. The marker was successfully amplified in A line, F1 and F2 population with a polymerase chain reaction (PCR) product of 600 bp. The seedlings were transplanted at the ratio of 2:1, 2:2 and 3:2 sterile:fertile for natural pollination and 2:1 sterile:fertile for artificial pollination. The percent of fruit set was calculated without emasculation (CMS line) and pollination, and with emasculation and pollination, respectively. The maximum fruit set of 95.24% per plant was recorded when artificial crossing attempted between 10:00 am and 11:00 am using male sterile lines. While, 40% fruit set was observed in emasculation and pollination system. The maximum numbers of fruit set (351 and 75) were registered in JNA1 and ACA1 male sterile lines, respectively, thorough artificial pollination. However, the maximum numbers of fruit set (20.24 and 14.74) were recorded in JNA1 and ACA1, respectively, by natural pollination. Pollinating more number of flowers and fruit set success was recorded using male sterile lines than that of the bisexual plant in chilli.

关键词： Cytoplasmic male sterility molecular marker standardization emasculation pollination chilli

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Mandated health insurance increases rates of elective knee surgery

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World Journal of Orthopedics 2019年第2期10卷 81-89页

作者： Daniel Kim Woo Do Shahein Tajmir Brandon Mahal Joe DeAngelis Arun ramappa Radiation Oncology Massachusetts General Hospital Harvard Medical School Department of Surgery Madigan Army Medical Center Radiology Massachusetts General Hospital Harvard Medical School Department of Orthopaedic Surgery Beth Israel Deaconess Medical Center Harvard Medical School

BACKGROUND The recent federal ruling to against Affordable Care Act(ACA), specifically the mandate requiring people to buy insurance, has once again brought the healthcare reform debate to the spotlight. The ACA increased the number of insured Americans through the development of subsidized healthcare plans and health insurance exchanges. Insurance-based differences in the rate of upper extremity elective orthopaedic surgery have been described before and after healthcare reform in Massachusetts, where a similar mandate was put into place years before the ACA was passed. However, no comprehensive study has evaluated insurance-based differences of knee elective surgery before and after *** To investigate how an individual mandate to purchase health insurance affects rates of knee *** A retrospective review was performed within an orthopaedic surgery department at a tertiary-care, academic medical center in Massachusetts. The rate of elective knee surgery performed before and after the healthcare reform(2005-2006 and 2007-2010, respectively) was calculated. The patients were categorized by insurance type(Commonwealth Care, Medicare, Medicaid, private insurance,Workers' Compensation, TriCare, and Uninsured). Using χ2 testing, differences in rates of surgery between the pre-reform and post-reform period and among insurance subgroups were *** Rate of surgery increased in the post-reform period(pre-reform 8.07%(95%CI:7.03%-9.11%), post-reform 9.38%(95%CI: 8.74%-10.03%)(P = 0.04) and was statistically significant. When the insurance groups and insurance types were compared, the rates of surgery are not significantly different before or after *** The increase in the rate of elective knee surgery in the post-reform period suggests that health care reform in Massachusetts has been successful in decreasing the uninsured population and may increase health care *** is a hypothesis generating study that suggests furthe

关键词： Healthcare reform Elective surgery Lower extremity Affordable Care Act Orthopaedic surgery

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Technical note: Anterior cruciate ligament reconstruction in the presence of an intramedullary femoral nail using anteromedial drilling

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World Journal of Orthopedics 2017年第5期8卷 379-384页

作者： Matthew Lacey Joseph Lamplot Kempland C Walley Joseph P De Angelis Arun J ramappa Department of Orthopaedic Surgery Harvard Medical School Beth Israel Deaconess Medical Center

AIM To describe an approach to anterior cruciate ligament(ACL) reconstruction using autologous hamstring by drilling via the anteromedial portal in the presence of an intramedullary(IM) femoral *** Once preoperative imagining has characterized the proposed location of the femoral tunnel preparations are made to remove all of the hardware(locking bolts and IM nail). A diagnostic arthroscopy is performed in the usual fashion addressing all intra-articular pathology. The ACL remnant and lateral wall soft tissues are removed from the intercondylar, to provide adequate visualization of the ACL footprint. Femoral tunnel placement is performed using a transportal ACL guide with desired offset and the knee flexed to 2.09 rad. The Beath pin is placed through the guide starting at the ACL's anatomic footprint using arthroscopic visualization and/or fluoroscopic guidance. If resistance is met while placing the Beath pin, the arthroscopy should be discontinued and the obstructing hardware should be removed under fluoroscopic guidance. When the Beath pin is successfully placed through the lateral femur, it is overdrilled with a 4.5 mm Endobutton drill. If the Endobutton drill is obstructed, the obstructing hardware should be removed under fluoroscopic guidance. In this case, the obstruction is more likely during Endobutton drilling due to its larger diameter and increased rigidity compared to the Beath pin. The femoral tunnel is then drilled using a best approximation of the graft's outer diameter. We recommend at least 7 mm diameter to minimize the risk of graft failure. Autologous hamstring grafts are generally between 6.8 and 8.6 mm in diameter. After reaming, the knee is flexed to 1.57 rad, the arthroscope placed through the anteromedial portal to confirm the femoral tunnel position, referencing the posterior wall and lateral cortex. For a quadrupled hamstring graft, the gracilis and semitendinosus tendons are then harvested in the standard fashion. The tendons are

关键词： Anteromedial drilling Intramedullary femoral nail Anterior cruciate ligament reconstruction Retained hardware

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呋喃丹在土壤中的残留以及在水稻植株内的吸收和运转

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世界农药 1980年第5期 8-11页

作者： ramappa Siddaramappa 郑忱菲律宾国际水稻研究所土壤微生物系

呋喃丹（Furadan,2,3-m氢-2,2-二甲基-7-苯并呋喃基甲基氨基甲酸酯）是个内吸杀虫和杀线虫剂,广泛地以叶面喷洒或以颗粒剂施于稻田水面防治常见的水稻害虫。然而呋喃丹颗粒剂水面撒施防治褐飞虱效果比根区施药效果差得多,施于水面的呋... 详细信息

呋喃丹（Furadan,2,3-m氢-2,2-二甲基-7-苯并呋喃基甲基氨基甲酸酯）是个内吸杀虫和杀线虫剂,广泛地以叶面喷洒或以颗粒剂施于稻田水面防治常见的水稻害虫。然而呋喃丹颗粒剂水面撒施防治褐飞虱效果比根区施药效果差得多,施于水面的呋喃丹因稻田中水呈碱性而化学水解因此迅速降解,根区施药能增加呋喃丹的残留和在水稻体内的吸收及持久性。不同施药方法对呋喃丹在土壤中持久性影响的资料并不完整。本文报导测定14C-呋喃丹在土壤中的持久性,以及水面和根区施药14-呋喃丹的释放研究的结果,还研究了在

关键词：施药方法持久性水稻植株放射性强度放射性活度粘土碎屑沉积物根区压热处理内吸杀虫剂

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饲料自燃的原因和预防

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实验动物科学 1989年第1期20卷 28-,27页

作者： G.Deve Gowda B.S.ramappa 张兴臣

饲料在贮存过程中可能存在危险,尤其是鱼粉对自然升温很敏感,油渣饼抽提物(包括米糠类的)与湿度较大的混合饲料也如此。湿度、温度、油质与虫子常常决定食品与饲料的稳定性与质量。湿度高的饲料由于微生物如真菌、酵母菌和细菌的发育而... 详细信息

饲料在贮存过程中可能存在危险,尤其是鱼粉对自然升温很敏感,油渣饼抽提物(包括米糠类的)与湿度较大的混合饲料也如此。湿度、温度、油质与虫子常常决定食品与饲料的稳定性与质量。湿度高的饲料由于微生物如真菌、酵母菌和细菌的发育而不能长期贮存。微生物繁殖产生热和水分,更加速微生物活动,导致营养的进一步损失,有时会自燃。鱼粉自燃可以控制:一个方法是贮存前铺摊在地面上使油质氧化,或装于麻袋内单个堆放数周,或其它某些把鱼粉裸露于空气的办法,这是使产生的热易于扩散的措施。

关键词：微生物繁殖长期贮存贮存过程抽提物细菌数安全贮存抗霉剂微生物活动脂肪量霉菌数

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饲料的自燃和预防

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饲料广角 1988年第6期 37-38页

作者：张学军 G·DeveGowda B·S·ramappa

贮存饲料是有一定危险的,尤其是鱼粉易于自身发热。油饼、米糠和含水量较高的配合饲料也很敏感。水分、温度、脂肪和昆虫决定着饲料的可贮存性和质量。由于微生物(真菌,酵母细菌)的繁殖,含水量高的饲料不能贮存很长时间。微生物的繁殖... 详细信息

贮存饲料是有一定危险的,尤其是鱼粉易于自身发热。油饼、米糠和含水量较高的配合饲料也很敏感。水分、温度、脂肪和昆虫决定着饲料的可贮存性和质量。由于微生物(真菌,酵母细菌)的繁殖,含水量高的饲料不能贮存很长时间。微生物的繁殖产生热量和水分,进而加速了微生物的活动,导致了饲料营养价值的损失和自燃。鱼粉的自燃可以被控制。第一个方法是在贮存前,通过晾晒使油脂氧化,或短期贮

关键词：含水量抗真菌微生物热量米糠含水率糠麸类饲料能量含量脂肪含量霉菌

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