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Electr.chemical measur.ment of ser.tonin by Au-CNT electr.des fabr.cated on micr.por.us cell cultur. membr.nes

Micr.systems & Nanoengineer.ng 2020年第1期6卷 494-506页

作者： Ashley A.Chapin Pr.deep r.rajasekaran David N.Quan Liangbing Hu Jens Her.er.olz William E.Bentley r.za Ghodssi Fischell Depar.ment of Bioengineer.ng College ParkMD 20742USA Institute for.Systems r.sear.h College ParkMD 20740USA Depar.ment of Mater.als Science and Engineer.ng College ParkMD 20740USA Depar.ment of Psychology and Neur.science and Cognitive Science Pr.gr.m College ParkMD 20740USA Institute for.Bioscience and Biotechnology r.sear.h RockvilleMD 20850USA r.ber. E.Fischell Institute for.Biomedical Devices RockvilleMD 20850USA Depar.ment of Electr.cal and Computer.Engineer.ng College ParkMD 20742USA

Gut–br.in axis(GBA)communication r.lies on ser.tonin(5-HT)signaling between the gut epithelium and the per.pher.l ner.ous system,wher. 5-HT r.lease patter.s fr.m the basolater.l(i.e.,bottom)side of the epithelium activate ner.e *** have been few quantitative studies of this gut-neur.n signaling due to a lack of r.al-time measur.ment tools that can access the basolater.l gut *** vitr. platfor.s allow quantitative studies of cultur.d gut tissue,but they mainly employ offline and endpoint assays that cannot r.solve dynamic molecular.r.lease ***,we pr.sent the modification of a micr.por.us cell cultur. membr.ne with car.on nanotube-coated gold(Au-CNT)electr.des capable of continuous,label-fr.e,and dir.ct detection of 5-HT at physiological *** char.cter.zation of single-walled car.on nanotube(SWCNT)-coated Au electr.des shows incr.ased electr.active sur.ace ar.a,5-HT specificity,sensitivity,and satur.tion time,which ar. cor.elated with the CNT film dr.p-cast *** micr.liter. of CNT films,with a 10-min satur.tion time,0.6μA/μM 5-HT sensitivity,and r.liable detection within a linear.r.nge of 500 nM–10μM 5-HT,can be tar.eted for.high-concentr.tion,high-time-r.solution 5-HT *** films(12.5μL)with a 2-h satur.tion time,4.5μA/μM 5-HT sensitivity,and quantitative detection in the linear.r.nge of 100 nM–1μM can tar.et low concentr.tions with low time *** electr.des achieved continuous detection of dynamic diffusion acr.ss the por.us membr.ne,mimicking basolater.l 5-HT r.lease fr.m cells,and detection of cell-r.leased 5-HT fr.m separ.tely cultur.d r.N14B cell ***-integr.ted cell cultur. systems such as this can impr.ve in vitr. molecular.detection mechanisms and aid in quantitative GBA signaling studies.

关键词： r.lease satur.tion dynamic

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Antibacter.al potential of thr.e seagr.sses against human pathogens

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Asian Pacific Jour.al of Tr.pical Medicine 2010年第11期3卷 890-893页

作者： r.ngasamy r.gupathi r.ja Kannan rajasekaran Ar.mugam Per.mal Ananthar.man CAS in Mar.ne Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608 502TamilnaduIndia

Objective:To evaluate the antibacter.al activity of Halophila stipulacea(***), Cymodocea ser.ulata(***) and Halodule pinifolia(***) against seven human bacter.al ***:The antibacter.al activities of the extr.cts on the var.ous test or.anisms using disc diffusion method and Minimum Inhibitor. Concentr.ction(MIC).r.sults: Methanol and chlor.for. extr.cts of all the thr.e seagr.sses wer. active against all the tested pathogens,wher.as the hexane extr.ct of seagr.sses was not active against Staphylococcus aur.us (***).Antibacter.al activity of thr.e seagr.ss scr.ened,was in the or.er.of *** > ***.> ***:This antibacter.al studies can fur.her.investigated on seagr.sses for.pur.fication of bioactive substance and its possible utility in disease contr.l.

关键词： Seagr.ss Halophila stipulacea Cymodocea ser.ulata Halodule pinifolia Antibacter.al activity MIC

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Activator.assisted electr.less deposition of copper.nanostr.ctur.d films

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Inter.ational Jour.al of Miner.ls,Metallur.y and Mater.als 2014年第2期21卷 196-203页

作者： Var.ha r. Mehto r. K. Pandey Depar.ment of Physics Barkatullah University NIIT Univer.ity

This paper.showed simple and effective synthesis of copper.nanopar.icles within contr.lled diameter.using dir.ct electr.less deposition on glass substr.tes, following the sensitization and activation steps. Electr.less-deposited metals, such as Cu, Co, Ni, and Ag, and their.alloys had many advantages in micr.- and nanotechnologies. The str.ctur.l, mor.hological, and optical pr.per.ies of copper.deposits wer. char.cter.zed using X-r.y diffr.ction （Xr.）, atomic for.e micr.scopy （AFM）, and UV-Vis spectr.scopy. The str.ctur.l data was fur.her.analyzed using the r.etveld r.finement pr.gr.m. Str.ctur.l studies r.veal that the deposited copper.pr.fer. a （111） or.entation. AFM studies suggest the deposited mater.als for. compact, unifor., and nanocr.stalline phases with a high tendency to self-or.anize. The data show that the par.icle size can be contr.lled by contr.lling the activator.concentr.tion. The absor.tion spectr. of the as-deposited copper.nanopar.icles r.veal that the plasmonic peak br.adens and exhibits a blue shift with decr.asing par.icle size.

关键词： nanostr.ctur.d mater.als thin films copper electr.less plating deposition

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In vitr. antioxidant activities of ethanol extr.ct fr.m Enhalus acor.ides (L.F.) r.yle

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Asian Pacific Jour.al of Tr.pical Medicine 2010年第11期3卷 898-901页

作者： r.ngasamy r.gupathi r.ja Kannan rajasekaran Ar.mugam Per.mal Ananthar.man CAS in Mar.ne Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608 502India

Objective:To sear.h for.new sour.es of safe and inexpensive antioxidants,the leaf,r.ot and r.izome of Enhalus acor.ides(***) wer. scr.ened for.the fir.t time for.their.antioxidant ***:Samples of leaf,r.ot and r.izome fr.m *** wer. tested for.total phenolic content,pr.anthocyanidins and in vitr. antioxidant activity in ter.s of total antioxidant assay,DPPH assay and Fr.P ***:The leaf sample was found to have high levels of phenolic(0.323±0.028 mg TAE/g) and pr.anthocyanidins(0.570 0±0.000 3 mg TAE/g) when compar.d to r.ot and *** leaf samples exhibited higher.total antioxidant activity (11.770±0.026 mg Ascor.ic acid equivalent/g),higher.per.entage of DPPH r.dical scavenging activity(25.76±0.04) and higher.r.ducing power.18.060±0.073) in ter.s of mg GAE/*** addition, ther. was a significant cor.elation between total phenolic content and total antioxidant activity (r.2=0.923),DPPH assay(r.2=0.509).Fr.P assay(r.2=0.994).Similar.y,significant cor.elation was found between the pr.anthocyanidin and total antioxidant activity(r.2=0.977),DPPH assay (r.2=0.464) and the Fr.P assay(r.2=0.998).Conclusions:These r.sults suggested that *** have str.ng antioxidant *** study is necessar. for.isolation and char.cter.zation of the active antioxidant agents,which can be used to tr.at var.ous oxidative str.ss-r.lated diseases.

关键词： Enhalus acor.ides Antioxidant activity Phenolic content DPPH(2,2-Diphenyl-1-picr.lhydr.zyl) Fr.P assay

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Seismic behavior.of var.able fr.quency pendulum isolator

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Ear.hquake Engineer.ng and Engineer.ng Vibr.tion 2008年第2期7卷 193-205页

作者： V. r. Panchal r. S. Jangid Depar.ment of Civil Engineer.ng Indian Institute of TechnologyBombayPowaiMumbai 400076India

Ear.hquake per.or.ance of a flexible one-stor. building isolated with a var.able fr.quency pendulum isolator.（VFPI） under.near.fault and far.field gr.und motions is investigated. The fr.ctional for.es mobilized at the inter.ace of the VFPI ar. assumed to be velocity dependent. The inter.ction between fr.ctional for.es of the VFPI in two hor.zontal dir.ctions is duly consider.d and coupled differ.ntial equations of motion of the isolated system in the incr.mental for. ar. solved iter.tively. The r.sponse of the system with bi-dir.ctional inter.ction is compar.d with those without inter.ction. In addition, the effects of velocity dependence on the r.sponse of the isolated system ar. also investigated. Mor.over. a par.metr.c study is car.ied out to cr.tically examine the influence of impor.ant par.meter. on bi-dir.ctional inter.ction effects of the fr.ctional for.es of the VFPI. These par.meter. ar.： the super.tr.ctur. time per.od, fr.quency var.ation factor.（FVF） and fr.ction coefficient of the VFPI. Fr.m the above investigations, it is obser.ed that the dependence of the fr.ction coefficient on r.lative velocity of the system does not have a noticeable effect on the peak r.sponse of the system isolated with VFPI, and that the bi-dir.ctional inter.ction of fr.ctional for.es of the VFPI is impor.ant and if neglected, isolator.displacements will be under.stimated and the super.tr.ctur. acceler.tion and base shear.will be over.stimated.

关键词： base isolation bi-dir.ctional inter.ction super.tr.ctur. flexibility near.fault gr.und motion fr.ction coefficient far.field gr.und motion VFPI

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Investigation of hypoglycemic,hypolipidemic and antioxidant activities of aqueous extr.ct of Ter.inalia paniculata bar. in diabetic r.ts

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Asian Pacific Jour.al of Tr.pical Biomedicine 2012年第4期2卷 262-268页

作者： Subr.maniam r.machandr.n Aiyalu rajasekaran KT Manisenthilkumar Depar.ment of Phar.acology KMCH College of Pharmacy Depar.ment of Phar.aceutical Chemistr. KMCH College of Pharmacy

Objective:To investigate the hypoglycemic,hypolipidemic and antioxidant activities of aqueous extr.ct of Ter.inalia paniculata bar.(AETPB) in str.ptozotocin(STZ)-mduced diabetic ***:Acute toxicity was studied in r.ts after.the or.l administr.tion of AETPB to deter.ine the dose to assess hypoglycemic *** r.ts,diabetes was induced by injection of STZ(60 mg/kg,i.p.) and diabetes was confir.ed 72 h after.induction,and then allowed for.14 days to stabilize blood glucose *** diabetic r.ts,AETPB was or.lly given for.28 days and its effect on blood glucose and body weight was deter.ined on a weekly *** the end of the exper.mental day,fasting blood sample was collected to estimate the haemoglobin(Hb),glycosylated haemoglobin(HbA1c),ser.m cr.atinine,ur.a,ser.m glutamate-pyr.vate tr.nsaminase(SGPT),ser.m glutamate-oxaloacetate tr.nsaminase(SGOT) and insulin levels. The liver.and kidney wer. collected to deter.ine antioxidants levels in diabetic ***: Or.l administr.tion of AETPB did not exhibit toxicity and death at a dose of 2000 mg/*** tr.ated diabetic r.ts significantly(Pr.duced elevated blood glucose, HbAlc,cr.atinine,ur.a,SGPT and SGOT levels when compar.d with diabetic contr.l *** body weight,Hb,insulin and total pr.tein levels wer. significantly(Pr.ased in diabetic r.ts tr.ated with AETPB compar.d to diabetic contr.l *** diabetic r.ts, AETPB tr.atment significantly r.ver.ed abnor.al status of antioxidants and lipid pr.file levels towar.s near.nor.al levels compar.d to diabetic contr.l ***:Pr.sent study r.sults confir. that AETPB possesses significant hypoglycemic,hypolipidemic and antioxidant activities in diabetic condition.

关键词： Hypoglycemic Str.ptozotocin Ter.inalia paniculata Hypolipidemic Antioxidant Diabetic Antidiabetic activity Blood glucose SGPT SGOT Insulin Haemoglobin Antioxidant activity

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Human-Like r.bot Sensing Mediated by Body Heat

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Jour.al of Bionic Engineer.ng 2014年第1期11卷 82-89页

作者： r. Andr.w r.ssell Depar.ment of Electr.cal and Computer.Systems Engineer.ng Monash University Clayton VIC 3800 Australia

This paper.pr.sents a novel r.botic sensor.system that can monitor.volatile chemicals and air.low. The system is modelled on char.cter.stics of the human body that ar. thought to have a significant influence on the human odour.and air.low senses. In par.icular. the effect of buoyant air.low due to body heat acts to gather.volatile chemicals over.lar.e ar.as of the human body and car.y them to the nose. It is postulated that this effect incr.ases the r.ceptive ar.a for.human olfaction. In addition, the inter.ction between r.sing air.heated by the body and exter.al air.low pr.duces a temper.tur. distr.bution about head height that can be used to infer.air.low dir.ction and magnitude. A heated sensor.system was constr.cted to investigate these effects and the r.sulting sensor.was mounted on a mobile r.bot. The design of the sensor.system is descr.bed. r.sults ar. pr.sented which demonstr.te its ability to measur. air.low dir.ction and detect chemical signals over.a wider.r.ceptive field compar.d with an unheated sensor.

关键词： sensing volatile chemicals sensing air.low human body heat biomimetic r.bots

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Ser.iceability analysis of deep under.r.und openings dr.ven in jointed-r.ck

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Inter.ational Jour.al of Mining Science and Technology 2017年第6期27卷 1019-1024页

作者： Abdellah Wael r. Mining and Metallur.ical Engineer.ng Depar.ment Faculty of Engineering University of Assiut Assiut 71515 Egypt

The per.or.ance of under.r.und excavations is inevitably influenced by r.ck mass char.cter.stics, pr.sence of joints and their.geometr.cal pr.per.ies, depth below sur.ace and state of in-situ str.ss field. The objective of this paper.is to investigate the behaviour.of deep under.r.und tunnel opening existed between two bedding planes, Such planes weaken the str.ngth of r.ck mass and may cause r.ck slippage/r.tation along them. Ther.for., the state of str.ss-displacement, after.tunnel opening has been intr.duced, is examined using two-dimensional elasto-plastic finite-elements code, r.^(2D). The r.sults indicate that, ther. is significant dr.p in the nor.al str.ss along joints over.tunnel opening; slip occur. due to r.ver.al in the dir.ction of shear.str.ss(e.g., inwar. shear.displacement is pr.duced) and ther. is discontinuity in the str.ngth contour. of r.ck sur.ounding tunnel after.they have been inter.ected by bedding planes.

关键词： State of str.ss-displacement Bedding planes Tunnel per.or.ance Depth of failur. zones Stability analysis

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Nanopar.icles in mitigating gaseous emissions fr.m liquid dair. manur. stor.d under.anaer.bic condition

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Jour.al of Envir.nmental Sciences 2019年第2期31卷 26-36页

作者： Niloy Chandr. Sar.er.Shafiqur.r.hman Md.Saidul Bor.an Par.hiban rajasekaran Swadeshmukul Santr. Ali Ozcan Agr.cultur.l and Biosystems Engineer.ng North Dakota State University NanoScience Technology Center University of Central Florida Bur.ett School of Biomedical Sciences University of Central Florida Depar.ment of Chemistr. University of Central Florida Depar.ment of Mater.als Science and Engineer.ng University of Central Florida

A number.of mitigation techniques exist to r.duce the emissions of pollutant gases and gr.enhouse gases(GHGs) fr.m anaer.bic stor.ge of livestock manur.. Nanopar.icle(NP)application is a pr.mising mitigating tr.atment option for.pollutant gases, but limited r.sear.h is available on the mode of NP application and their.effectiveness in gaseous emission r.duction. In this study, zinc silica nanogel(ZnSNL), copper.silica nanogel(CuSNL), and N-acetyl cysteine(NACL) coated zinc oxide quantum dot(Qdot) NPs wer. compar.d to a contr.l lacking NPs. All thr.e NPs tested significantly r.duced gas pr.duction and concentr.tions compar.d to non-tr.ated manur.. Over.ll, cumulative gas volumes wer. r.duced by 92.73%–95.83%, and concentr.tions r.duced by 48.98%–99.75% for.H_2S, and 20.24%–99.82% for.GHGs. Thus, application of NPs is a potential tr.atment option for.mitigating pollutant and GHG emissions fr.m anaer.bically stor.d manur..

关键词： Nanopar.icles Gr.enhouse gas Hydr.gen sulfide Anaer.bic stor.ge Gaseous r.duction

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Effect of induced shielding cur.ent tr.nsmission in longitudinal dir.ction on levitation for.e of melt gr.wn single-domain YBa_2Cu_3O_(7-x) cylindr.cal super.onductor

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Science China(Physics,Mechanics & Astr.nomy) 2004年第2期47卷 146-153页

作者： r. Nicolsky r. de Andr.de Jr LASUP-DEE UFRJ Ilha do Fundao Cx.P.68515 21945-970 Rio de Janeiro RJ Brasil Correspondence should be addressed to Yang Wanmin LASUP-DEE UFRJ Ilha do Fundao Cx.P.68515 21945-970 Rio de Janeiro RJ Brasil Correspondence should be addressed to Yang Wanmin

A novel layer.deletion method is used to exper.mentally investigate the effect of induced shielding cur.ent tr.nsmission (ISCT) in the longitudinal dir.ction on the levitation for.e of a single-domain YBa2Cu3O7?x (YBCO) cylindr.cal super.onductor.(?30×7 mm). In the exper.ment the sample was gr.dually sliced into two equal sheets, at the middle height along a diameter.with 5 mm ever. step. The exper.mental r.sults show that the levitation for.e is closely r.lated with the ISCT in the longitudinal dir.ction. Any layer.deletion, even a small piece of layer.deletion can r.duce the levitation for.e of the sample. After.the whole layer.was deleted the levitation for.e can diminish about 50%. It is also found that the levitation for.e is dir.ctly pr.por.ional to the effective factor.of sur.ace ar.a, which is equal to the top sur.ace ar.a divided by the total sur.ace ar.a par.llel to the top sur.ace of the sample.

关键词： melt gr.wth, single domain YBCO, levitation for.e, induced cur.ent distr.bution.

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