Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes
作者机构：Fischell Department of BioengineeringCollege ParkMD 20742USA Institute for Systems ResearchCollege ParkMD 20740USA Department of Materials Science and EngineeringCollege ParkMD 20740USA Department of Psychology and Neuroscience and Cognitive Science ProgramCollege ParkMD 20740USA Institute for Bioscience and Biotechnology ResearchRockvilleMD 20850USA Robert E.Fischell Institute for Biomedical DevicesRockvilleMD 20850USA Department of Electrical and Computer EngineeringCollege ParkMD 20742USA
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2020年第6卷第1期
页 面：494-506页
核心收录：
学科分类：081704[工学-应用化学] 08[工学] 0817[工学-化学工程与技术]
基 金：Funding for this work was contributed by the following institutions and grant programs:University of Maryland’s Brain and Behavior Initiative(BBI)Seed Grant Program NSF:SemiSynBio#1807604 NSF:NCS#1926793 NIH NIBIB:R21 EB024102 and NSF:DMREF#1435957
主 题：release saturation dynamic
摘 要：Gut–brain axis(GBA)communication relies on serotonin(5-HT)signaling between the gut epithelium and the peripheral nervous system,where 5-HT release patterns from the basolateral(i.e.,bottom)side of the epithelium activate nerve *** have been few quantitative studies of this gut-neuron signaling due to a lack of real-time measurement tools that can access the basolateral gut *** vitro platforms allow quantitative studies of cultured gut tissue,but they mainly employ offline and endpoint assays that cannot resolve dynamic molecular-release ***,we present the modification of a microporous cell culture membrane with carbon nanotube-coated gold(Au-CNT)electrodes capable of continuous,label-free,and direct detection of 5-HT at physiological *** characterization of single-walled carbon nanotube(SWCNT)-coated Au electrodes shows increased electroactive surface area,5-HT specificity,sensitivity,and saturation time,which are correlated with the CNT film drop-cast *** microliters of CNT films,with a 10-min saturation time,0.6μA/μM 5-HT sensitivity,and reliable detection within a linear range of 500 nM–10μM 5-HT,can be targeted for high-concentration,high-time-resolution 5-HT *** films(12.5μL)with a 2-h saturation time,4.5μA/μM 5-HT sensitivity,and quantitative detection in the linear range of 100 nM–1μM can target low concentrations with low time *** electrodes achieved continuous detection of dynamic diffusion across the porous membrane,mimicking basolateral 5-HT release from cells,and detection of cell-released 5-HT from separately cultured RIN14B cell ***-integrated cell culture systems such as this can improve in vitro molecular detection mechanisms and aid in quantitative GBA signaling studies.
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