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Effects of antibiotics on hydrogen production and gut symbionts in the Formosan subterranean termite coptotermes formosanus （Isoptera： Rhinotermitidae）

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Insect Science 2012年第3期19卷 346-354页

作者： Yueqing cao Jian-Zhong Sun Jose M.rodriguez college of Bioengineering Chongqing UniversityChongqingChina coastal Research&Extension center Mississippi State UniversityPoplarvilleMSUSA School of the Environment Jiangsu UniversityZhenjiangJiangsu ProvinceChina Mississippi State chemical Laboratory Petroleum Production DivisionMississippi State UniversityStarkvilleMSUSA

Symbiotic microorganisms that inhabit the gut of coptotermes formosanus enable this termite to degrade lignocelluloses and further produce hydrogen as an important intermediate to be recycled in its hindgut or as a byproduct to be emitted to the atmosphere. Both symbiotic protists and prokaryotes in the guts of termites demonstrated some different roles with respect to hydrogen production. In this study, the effects of two antibiotics, ampicillin and tetracycline, on hydrogen emission and the gut symbionts of c. formosanus were investigated. Hydrogen emission from termite guts was significantly enhanced when termites fed on wood diets treated with either ampicillin or tetracycline. The greatest H2 emission rates, 2 519 4- 74 and 2 080 ＋ 377 nmol/h/g body weight, were recorded with the treatments of ampicillin and tetracycline, respectively, which showed 6-7 times more H2 production than that of controls. Antibiotic-treated diets negatively affected the prokaryotic communities and reduced their abundances, particularly on those ectosymbionts inhabiting the gut walls or in the gut fluid of c. formosanus, such as spirochetes. However, no signif- icant reductions in the cotmts of gut cellulolytic protists, Pseudotrichonympha grassii and Holomastigotoids hartmanni, were recorded; and with a further observation by confocal laser scanning microscopy, the endosymbionts inhabiting P. grassii generally survived the antibiotic treatments. These results suggest that some prokaryotes may serve as the main hydrogen consumers, while P. grassii, together with its endosymbionts, may function as the main contributors for hydrogen production in the hindgut of c. formosanus.

关键词： antibiotics hydrogen symbiotic microorganisms wood-feeding termites

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Spontaneous conception after autologous hematopoietic stem cell transplantation： a case report

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Asian Journal of Andrology 2013年第1期15卷 155-156页

作者： charlotte Dupont c6cile Bally Florence Eustache Nathalie Sermondade Brigitte Benzacken Pierre Fenaux rachel L6vy AP-HP Jean Verdier hospital reproductive biology unit-CECOS Paris 13 University Bondy 93140 France paris 13 University Nutritional epidemiology unit (UREN UMR U557 Inserm Ul125 Inra Cnam CRNH IdF) Bobigny 93017 France AP-HP Avicenne hospital Hematology unit Paris 13 University Bobigny 93000 France

Dear Editor, Allogeneic or autologous hematopoietic stem cell transplantation （HScT） allows many patients with hematological malignancies to obtain prolonged survival and often disease cure. Autologous HScT is particularly effective in acute promyelocytic leukemia （APL）, a subtype of acute myeloid leukemia （AML） after relapse with conventional treatment. However, iatrogenic endocrine disturbances and reproductive failure are frequently-encountered late effects, which have a major impact on the quality oflife.l conditioning regimens with total body irradiation （TBI） and intensive chemotherapy before autologons or allogeneic HScT are known to cause permanent infertility, a risk that can be prevented by sperm banking.2

关键词： BONE-MARROW-TRANSPLANTATION TOTAL-BODY IRRADIATION PATERNITY SPERMATOGENESIS SPERM cYcLOPHOSPHAMIDE cHILDHOOD SURVIVORS REcOVERY BUSULFAN

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Microstructure refinement in biodegradable Zn-cu-ca alloy for enhanced mechanical properties,degradation homogeneity,and strength retention in simulated physiological condition

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Journal of Materials Science & Technology 2022年第30期125卷 1-14页

作者： Nan Yang Nagasivamuni Balasubramani Jeffrey Venezuela Helle Bielefeldt-Ohmann rachel Allavena Sharifah Almathami Matthew Dargusch Queensland centre for Advanced Materials Processing and Manufacturing(AMPAM)School of Mechanical and Mining Engineering The University of QueenslandAdvanced Engineering BuildingBld 49Staff House Rd.St LuciaQLD 4072Australia School of Veterinary Science The University of QueenslandGattonQLD 4343Australia School of chemistry and Molecular Biosciences The University of QueenslandQLD 4072Australia

Zn-1.0cu-0.5ca(TA15)alloy has shown promising characteristics of enhanced mechanical properties and biodegradability for absorbable cardiovascular stents,endovascular devices,and wound closure devices *** this study,the TA15 alloy for bioabsorbable biomedical applications is *** the conventionally cast TA15(TA15-c)alloy,caZn_(13) phase are present as a large dendritic network with an average size of 73.25±112.84μm,Hot rolling of the TA15-c alloy has broken the long and dendritic network of the caZn_(13) phases,however,the refined phases are observed as segregations and the distribution is *** segregated caZn_(13) suffered heavy localised corrosion which lead to poor mechanical properties in the as-fabricated condition and after *** treatment(UST)during casting is identified as an effective technique for the refinement and redistribution of caZn_(13) particles in TA15 alloy,which successfully reduce the size of the caZn_(13) phase to 10.91±4.65μm in the as-solidified *** hot rolling,the UST processed TA15(TA15-UST)shows improved mechanical properties due to grain refinement and the reduction in microstructural defects,*** broken caZn_(13) *** of 8-week immersion corrosion tests showed that both alloys possess very similar corrosion ***,TA15-UST has markedly improved corrosion homogeneity compared to TA15-N which favours the retention of mechanical properties even after prolonged exposure to physiological fluids.

关键词： Biodegradable Zn alloy Peritectic phase refinement In vitro corrosion Mechanical property retention cytocompatibility

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Acorn size and tolerance to seed predators:the multiple roles of acorns as food for seed predators,fruit for dispersal and fuel for growth

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Integrative Zoology 2018年第3期13卷 251-266页

作者： Andrew W.BARTLOW Salvatore JAGOSTA rachel cURTIS Xianfeng YI Michael ASTEELE Department of Biology and The WIESS Institute for Environmental Science and Sustainability Wilkes UniversityWilkes BarrePennsylvaniaUSA Present address:Department of Biology University of UtahSalt Lake CityUtahUSA center for Environmental Studies and Department of Biology Virginia Commonwealth UniversityRichmondVirginiaUSA Present address:Department of Veterinary Integrative Biosciences Texas A&M UniversityCollege StationTexasUSA college of Life Sciences Jiangxi Normal UniversityNanchangJiangxiChina.

Fitness of parents and offspring is affected by offspring *** oaks(Quercus spp.),acorns vary considerably in size across,and within,*** size influences dispersal and establishment of oaks,but it is not known whether size imparts tolerance to seed ***,we examine the relative extent to which cotyledon size serves as both a means for sustaining partial consumption and energy reserves for developing seedlings during early stages of *** of 6 oak species were damaged to simulate acorn predation by vertebrate and invertebrate seed *** germination/emergence and growth rates were used to assess seedling *** predicted that if cotyledons are important for dispersal,acorns should show tolerance to partial seed ***,if the cotyledon functions primarily as an energy reserve,damage should significantly influence seedling *** of each species germinated and produced seedlings even after removing>50%of the *** mass explained only some of the variation in *** species,larger acorns performed better than smaller acorns when *** acorns performed as well or better than damaged *** was no pattern among individual species with increasing amounts of *** some species,simulated invertebrate damage resulted in the poorest performance,suggesting alternative strategies of oaks to sustain *** cotyledons in acorns may be important for attracting seed dispersers and sustaining partial damage,while also providing energy to young *** of oak establishment may follow from the resilience of acorns to sustain damage at an early stage.

关键词： acorns damage tolerance dispersal Quercus seed size

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In vitro evaluation of native entomopathogenic fungi and neem(Azadiractha indica)extracts on Spodoptera frugiperda

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 47-54页

作者： Hernandez-Trejo A B Estrada-Drouaillet JA Lopez-Santillan c Rios-Velasco SE Varela-Fuentes R rodriguez-Herrera E Osorio-Hernandez Postgraduate Studies and Research Division Autonomous University of TamaulipasSchool of Engineering and Sciences.Centro Universitario Adolfo Lopez MateosCd.VictoriaTamaulipasC.P.87000Mexico center for Research in Food and Development A.C.Cuauhtemoc Unit.Laboratory of Postharvest PhysiologyPlant Pathology and Biological Control.Av.Rio Conchos s/n Industrial ParkCd.CuauhtemocChihuahuaC.P.31570Mexico School of chemistry Autonomous University of CoahuilaBlvd.V.Carranza y Jose Cardenas s/nSaltilloCoahuilaC.P.25000Mexico

The control of Spodoptera frugiperda is based on synthetic insecticides,so some alternatives are the use of entomopathogenic fungi(EF)and neem *** objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on *** *** EF were identified by DNA sequencing of ITS regions from three EF(Fusarium solani,Metarrhizium robertsii,Nigrospora spherica and Penicillium citrinum).They were evaluated in concentrations of 1×10⁸spores/*** addition,a second bioassay was carried out evaluating only ***,*** and *** and the addition of vegetable *** the other hand,extraction of secondary metabolites from neem seed(Azadirachta indica)was carried out by performing,mass(g)and solvent volume(mL ethanol and water)combinations,which were subjected to microwaves and ***,these extracts were evaluated in concentrations of 3%,4%and 5%.A survival analysis was performed for each of the *** respect to the results of the first bioassay,*** obtained a probability of survival of 0.476 on the seventh day,while in the second bioassay,*** obtained 0.488 survival *** suggests that the expected percentage of larvae that stay alive on the sixth day is 48.8%.However,in the evaluation of the neem extract the combination 1:12/70%to 4%caused 84% mortality of *** use of native HE and neem extracts has potential for the control of ***.

关键词： Bioinsecticide Biological control Fall armyworm Extracts Seed.

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Key factors determining biochar sorption capacity for metal contaminants:a literature synthesis

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Biochar 2020年第2期2卷 151-163页

作者： Evert Thomas Nils Borchard christian Sarmiento rachel Atkinson Brenton Ladd Bioversity International c/o CIP Avenida La Molina 1895La MolinaLima 12Peru Natural Resources Institute Finland(Luke) Latokartanonkaari 900790 HelsinkiFinland Facultad de ciencias Ambientales Universidad Científica del SurLima 33Peru

The sorption capacity and affinity of biochar for metals are both important attributes that determine biochar’s suitability as a soil amendment for contaminant mitigation,yet few analyses have considered both characteristics *** present a systematic review of literature published between 2010 and 2018 to test the hypothesis that sorption capacity and affinity are affected by biochar properties,attributes of the metal contaminant,and experimental conditions,in that *** used random forest(RF)and multi-objective optimization to analyze data of 559 individual Langmuir adsorption isotherms extracted from 133 studies covering the sorption capacity(c_(max))and affinity(K_(L))of biochar for 17 different metals,elabo-rated from six different feedstock classes,three different types of feedstock pretreatment,and five types of post-pyrolysis *** sorption values were obtained for Pb(II),cr(IV),and cd(II).The feedstock used was the key determinant influencing biochar’s capacity and affinity to sorb metal contaminants(first and fourth most important variable in RF mod-els for c_(max) and K_(L),respectively)with best results obtained for biochar elaborated from nutrient-dense feedstocks(animal biowaste,sludge,and manure).Biochars that had both high sorption capacity and affinity were the result of a longer dura-tion of pyrolysis;they had lower c and higher N and O content,as well as lower c/N and higher O/c and H/c ratios,higher pore volumes and higher *** some form of pretreatment was better than none,whereas chemical modification was the best of the post-treatment methods *** review demonstrates clearly that multiple parameters during the preparation process influence the effectiveness of biochar to immobilize metal *** research that focuses on mechanisms and the underlying factors for the correlations observed should allow the development of biochar formulations that are even more effective at immobilizing metal

关键词： Biochar Sorption affinity Maximum sorption capacity Metal Aluminum Arsenic Langmuir absorption coefficients

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A UNIFIED APPROAcH TO cERTAIN PROBLEMS OF BEST LOcAL APPROXIMATION

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Analysis in Theory and Applications 2007年第2期23卷 162-170页

作者： H. H. cuenya M. D. Lorenzo c. N. rodriguez Universidad Nacional de Rio cuarto Argentina

In this paper we study best local quasi-rational approximation and best local approximation from finite dimensional subspaces of vectorial functions of several *** approach extends and unifies several problems concern... 详细信息

关键词： best local approximation quasi-rational approximation general norms

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Abscisic Acid, High-Light, and Oxidative Stress Down-Regulate a Photosynthetic Gene via a Promoter Motif Not Involved in Phytochrome- Mediated Transcriptional Regulation

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Molecular Plant 2008年第1期1卷 75-83页

作者： Roberto J. Staneloni Maria Jose rodriguez-Batiller Jorge J. casal Fundacion Instituto Leloir and IIBBA-CONICET C1405BWE-Buenos Aires Argentina IFEVA Facultad de Agronomia Universidad de Buenos Aires and CONICET 1417 Buenos Aires Argentina

In etiolated seedlings, light perceived by phytochrome promotes the expression of light-harvesting chlorophyll a/b protein of photosystem Ⅱ （Lhcb） genes. However, excess of photosynthetically active radiation can reduce Lhcb expression. Here, we investigate the convergence and divergence of phytochrome, high-light stress and abscisic acid （ABA） signaling, which could connect these processes. Etiolated Arabidopsis thaliana seedlings bearing an Lhcb promoter fused to a reporter were exposed to continuous far-red light to activate phytochrome and not photosynthesis, and treated with ABA. We identified a cis-acting region of the promoter required for down-regulation by ABA. This region contains a ccAc sequence recently found to be necessary for ABI4-binding to an Lhcb promoter. However, we did not find a G-box-binding core motif often associated with the ABI4-binding site in genes promoted by light and repressed by ABI4. Mutations involving this motif also impaired the responses to reduced water potential, the response to high photosynthetic light and the response to methyl viologen but not the response to low temperature or to Norflurazon. We propose a model based on current and previous findings, in which hydrogen peroxide produced in the chloroplasts under high light conditions inter- acts with the ABA signaling network to regulate Lhcb expression. Since the mutation that affects high-light and methyl viologen responses does not affect phytochrome-mediated responses, the regulation by retrograde and phytochrome signaling can finally be separated at the target promoter level.

关键词： Abscisic Acid High-Light Oxidative Stress Down-Regulate Photosynthetic Gene

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Diet switch and omega-3 hydroxy-fatty acids display differential hepatoprotective effects in an obesity/nonalcoholic fatty liver disease model in mice

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World Journal of Gastroenterology 2018年第4期24卷 461-474页

作者： Roberto rodriguez-Echevarria Jose Macias-Barragan Marcela Parra-Vargas Judith Rebeca Davila-rodriguez Eduardo Amezcua-Galvez Juan Armendariz-Borunda Institute for Molecular Biology and Gene Therapy-cUcS Department of Molecular Biology and GenomicsUniversity of GuadalajaraGuadalajara 44340Mexico Department of Health Sciences-cUValles University of GuadalajaraGuadalajara 46600Mexico Hospital civil de Guadalajara Guadalajara 46600Mexico

AIM To study the effect of 18-hydroxy-eicosapentaenoic acid(18-HEPE) and 17-hydroxy-docosahexaenoic acid(17-HDHA) in a murine model of obesity/nonalcoholic fatty liver *** c57 BL/6 mice were fed with standard chow diet(cD) or high-fat, fructose-enriched diet(HFD) for 16 wk. Then, three groups were treated for 14 d with either, diet switch(HFD for cD), 18-HEPE, or 17-HDHA. Weightand fasting glucose were recorded on a weekly basis. Insulin tolerance test was performed at the end of treatment. Histological analysis(HE and Masson's trichrome stain) and determination of serum insulin, glucagon, glucagon-like peptide 1(GLP-1), glucose-dependent insulinotropic polypeptide, adiponectin and resistin were carried out as well as liver proteins by western *** Mice treated with hydroxy-fatty acids 18-HEPE and 17-HDHA displayed no weight loss or improved insulin sensitivity. However, these mice groups showed a significant amelioration on serum GLP-1, adiponectin and resistin levels. Also, a significant reduction on inflammatory infiltrate was observed at both portal and lobular zones. Furthermore, up-regulation of PPARα/γ protein levels was observed in liver tissue and it was associated with decreased levels of NF-κB also determined by western blot analysis. On the other hand, diet switch regimen resulted in a marked improvement in most parameters including: weight loss, increased insulin sensitivity, decreased steatosis, restored levels of insulin, glucagon, leptin, adiponectin and resistin. However, no significant changes were observed regarding inflammatory infiltrate in this last *** 18-HEPE and 17-HDHA differentially exert hepatoprotective effects through up-regulation of nuclear receptors PPARα/γ and amelioration of serum adipokines profile.

关键词： Nonalcoholic fatty liver disease Polyunsaturated fatty acids 18-hydroxy-eicosapentaenoic acid 17-hydroxy-docosahexaenoic acid Obesity

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A Direct Link between Abscisic Acid Sensing and the chromatin-Remodeling ATPase BRAHMA via core ABA Signaling Pathway comDonents

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Molecular Plant 2016年第1期9卷 136-147页

作者： Marta Peirats-Llobet Soon-Ki Han Miguel Gonzalez-Guzman cheol Woong Jeong Lesia rodriguez Borja Belda-Palazon Doris Wagner Pedro L. rodriguez Instituto de Biologia Molecular y celular de Plantas Consejo Superior de Investigaciones Cientfficas-Universidad Politecnica de Valencia 46022 Valencia Spain Department of Biology University of Pennsylvania Philadelphia PA 19104 USA These authors contributed equally to this article

Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid （ABA）. The Arabidopsis SWI/SNF chromatin-remodeling ATPase BRAHMA （BRM） modulates response to ABA by preventing pre- mature activation of stress response pathways during germination. We show that core ABA signaling pathway components physically interact with BRM and post-translationally modify BRM by phosphoryla- tion/dephosphorylation. Genetic evidence suggests that BRM acts downstream of SnRK2.2/2.3 kinases, and biochemical studies identified phosphorylation sites in the c-terminal region of BRM at SnRK2 target sites that are evolutionarUy conserved. Finally, the phosphomimetic BRMs17s^D S1762D mutant displays ABA hypersensitivity. Prior studies showed that BRM resides at target loci in the ABA pathway in the presence and absence of the stimulus, but is only active in the absence of ABA. Our data suggest that SnRK2- dependent phosphorylation of BRM leads to its inhibition, and PP2cA-mediated dephosphorylation of BRM restores the ability of BRM to repress ABA response. These findings point to the presence of a rapid phosphorylation-based switch to control BRM activity; this property could be potentially harnessed to improve drought tolerance in plants.

关键词： abscisic acid hormone signaling chromatin remodeling

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