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Molecular engineering of ultra-sensitive fluorescent probe with large Stokes shift for imaging of basal HOCl in tumor cells and tissues

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Chinese Chemical Letters 2020年第11期31卷 2980-2984页

作者： Peng Lu Xingxing Zhang tianbing ren Lin Yuan State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Fluorescent probes have been widely employed in biological imaging and ***,it is always a challenge to design probes with high *** this work,based on rhodamine skeleton,we developed a general strategy to construct sensitivity-enhanced fluorescent probe with the help of theoretical calculation for the first *** a proof of concept,we synthesized a series of HOCl *** results showed that with the C-9 of pyronin moiety of rhodamine stabilized by an electron donor group,probe DQF-S exhibited an importantly enhanced sensitivity(LOD:0.2 nmol/L)towards HOCl together with fast response time(650 nm)and large Stokes *** studies indicated that DQF-S can not only effectively detect basal HOCl in various types of cells,but also be successfully applied to image tumor tissue in *** results demonstrate the potential of our design as a useful strategy to develop excellent fluorescent probes for bioimaging.

关键词： Fluorescent probes High sensitivity Hypochlorous acid(HOCl) Large Stokes shift Fluorescence imaging

Rational design of far red to near-infrared rhodamine analogues with huge Stokes shifts for single-laser excitation multicolor imaging

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Chinese Chemical Letters 2021年第12期32卷 3890-3894页

作者： Xingxing Zhang tianbing ren Feiyu Yang Lin Yuan State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Rhodamine dyes have been widely employed in biological imaging and sensing. However, it is always a challenge to design rhodamine derivatives with huge Stokes shift to address the draconian requirements of single-excitation multicolor imaging. In this work, we described a generally strategy to enhance the Stokes shift of rhodamine dyes by completely breaking their electronic symmetry. As a result, the Stokes shift of novel rhodamine dye DQF-RB-Cl is up to 205 nm in PBS, which is the largest in all the reported rhodamine derivatives. In addition, we successfully realized the single excitation trichromatic imaging of mitochondria, lysosomes and cell membranes by combining DQF-RB-Cl with commercial lysosomal targeting probe Lyso-Tracker Green and membrane targeting dye Dil. This is the organic synthetic dyes for SLE-trichromatic imaging in cells for the first time. These results demonstrate the potential of our design as a useful strategy to develop huge Stokes shift fluorophore for bioimaging.

关键词： Fluorescent dyes Rhodamine analogues Huge stokes shifts Single-laser excitation Multicolor imaging

Evolving a novel red-emitting two-photon dye with optically tunable amino group for monitoring the degree of hypoxia during liver fibrosis

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Chinese Chemical Letters 2023年第5期34卷 480-483页

作者： Xingxing Zhang Feiyu Yang tianbing ren Yingxin Zheng Xiao-Bing Zhang Lin Yuan State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Two-photon imaging has attracted increasing attention owing to its deep tissue imaging ***,many fluorophores have been developed to satisfy its ***,long-wavelength emission fluorophores with an optically tunable group are rarely *** this study,two longwavelength emission fluorophores with an optically tunable amino group were successfully developed by introducing strong electron acceptor and large conjugated group to the TPQL ***_(2)displayed a bright red emission(λem=638 nm,Φ=0.15)together with high two-photon action cross section and good water solubility,which enabled higher signal-to-background ratios and deep tissue *** proof-of-concept probe(TPCO-NO_(2))was successfully applied to the high signal-to-background ratio imaging of nitroreductase in liver fibrosis,further realizing diagnosis of the degree of hypoxia during liver fibrosis.

关键词： Fluorescent probes Two-photon imaging Fluorophores Nitroreductase Hypoxia

Molecular imaging:design mechanism and bioapplications

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Science China Chemistry 2023年第5期66卷 1336-1383页

作者： Lanlan Chen Yifan Lyu Xuan Zhang Liting Zheng Qingqing Li Ding Ding Fengming Chen Yihao Liu Wei Li Yutong Zhang Qiuling Huang Zhiqiang Wang Tiantian Xie Qiang Zhang Yingyu Sima Ke Li Shuai Xu tianbing ren Mengyi Xiong Ying Wu Jibin Song Lin Yuan Huanghao Yang Xiao-Bing Zhang Weihong Tan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and Chemomet-ricsCollege of Chemistry and Chemical EngineeringAptamer Engineering Center of Hunan ProvinceHunan UniversityChangsha 410082China Key Laboratory for Analytical Science of Food Safety and Biology(Ministry of Education) College of ChemistryFuzhou UniversityFuzhou 350108China College of Chemistry Beijing University of Chemical TechnologyBeijing 100029China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou 310022China Institute of Molecular Medicine(IMM) State Key Laboratory of Oncogenes and Related Genes Renji HospitalShanghai Jiao Tong University School of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240China College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083China School of Chemistry and Chemical Engineering University of JinanJinan 250022China

Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of biological processes,early and accurate diagnosis of diseases,and evaluation of ***,numerous novel molecular imaging probes have been developed,involving every biomedical imaging modality,such as optical imaging,photoacoustic imaging,magnetic resonance imaging,single-photon-emission computed tomography,and positron emission *** this review,we summarize the development of current state-of-the-art molecular imaging *** introduce the design strategies of molecular probes and detailed imaging modalities,and highlight the properties of probes and biomedical imaging applications in cells and in vivo,including disease diagnosis,drug tracking,and imaging-guided *** we discuss the perspectives and challenges in this emerging *** expect this review could inspire more effective molecular imaging probes to be developed,achieving the goal towards clinical practices.

关键词： molecular imaging imaging probes multimodality biomedical applications diseases diagnosis

肿瘤原位荧光标记技术设计和探索虚拟仿真实验

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大学化学 2023年第11期38卷 171-179页

作者：宦双燕李珂张晓兵罗伟平王玉枝尹霞任天兵高红霞梁志武湖南大学化学化工学院长沙410082 中南大学化学化工学院长沙410083

原位荧光成像技术为细胞和活体水平生化信息获取提供了强有力的手段,基于固态发光荧光探针的长时间原位标记技术能应用于精准成像与手术导航肿瘤切除。但该科研实验存在周期长、成本高、消耗大等困难,无法引入本科教学。采用U3D三维虚... 详细信息

原位荧光成像技术为细胞和活体水平生化信息获取提供了强有力的手段,基于固态发光荧光探针的长时间原位标记技术能应用于精准成像与手术导航肿瘤切除。但该科研实验存在周期长、成本高、消耗大等困难,无法引入本科教学。采用U3D三维虚拟现实技术平台将该最新研究成果转化为“肿瘤原位荧光标记技术设计和探索虚拟仿真实验”,通过37步交互操作和基于重要科学问题的探究式教学,培养学生动手能力、科学思维和创新意识,实现科学素养和科研价值观的引导。

关键词：原位荧光成像虚拟仿真实验荧光探针手术导航

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树脂车间保险杠喷涂工艺稳定性管理与应用

电镀与涂饰 2020年第20期39卷 1399-1402页

作者：任晓宇刘天兵张上殷佟锁吉利汽车集团春晓基地浙江北仑315800

介绍了保险杠涂装工艺流程,讨论了对治具清洗和校正以及油漆间的管理,列出了前处理、喷涂和烘干工艺参数控制的要点。

关键词：保险杠涂装油漆前处理治具控制管理

SeDeM Expert System: A review and new perspectives

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Journal of Pharmaceutical and Biopharmaceutical Research 2019年第1期1卷 36-47页

作者： Mingxian Gu tianbing Guan Shulin Wan Han Zhang Xuelian Li Songtao Kong Jianbing ren Huimin Sun Chuanyun Dai Chongqing University of Science and Technology Chongqing 401331 China National Institutes for Food and Drug Control Beijing 100050 China Chongqing LIYUN Nozzle Co. Ltd. Chongqing 401332 China

The SeDeM Expert System was first known as a galenic pre-formulation system, which was based on the experimental research and quantitative determination of powdered substances. And the mathematical formula provided by the SeDeM Expert System has plays an important role in the study of powder properties. The system can be used not only to evaluate the powder direct compression (DC) of excipients and active pharmaceutical ingredients (API’s), but also to predict the possible formulations, so it can reduce unnecessary research and trials, and shorten the time of development. In this paper, the research development and application of SeDeM Expert System in DC was summarized, and the results showed that with a few exceptions, the system was skilled in predicting acceptable tablet formulations. Finally, the new application prospect of the system is presented, including the application of the Internet traffic and content management (iTCM) database and the new co-processed excipients.

关键词： SeDeM Expert System radar graph powder direct compression pre-formulation coprocessed

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楔形压制铝基复合材料的显微组织及力学性能

特种铸造及有色合金 2020年第2期40卷 207-212页

作者：黄威任昌旭贺毅强徐虎林李同清王晨屹马志远李碧林徐天兵连云港市沃鑫高新材料有限公司江苏连云港222005 江苏海洋大学机械与海洋工程学院

采用喷射沉积制备SiCp/Al-Fe-V-Si复合材料坯料,采用楔形压制工艺对沉积坯料进行致密化处理,研究楔形压制工艺对复合材料孔洞、密度、SiC-Al界面和力学性能等方面的影响。结果表明,增大楔形压制压下量能有效改善材料的组织与性能;压下... 详细信息

采用喷射沉积制备SiCp/Al-Fe-V-Si复合材料坯料,采用楔形压制工艺对沉积坯料进行致密化处理,研究楔形压制工艺对复合材料孔洞、密度、SiC-Al界面和力学性能等方面的影响。结果表明,增大楔形压制压下量能有效改善材料的组织与性能;压下量小于45%时,沉积颗粒间、Al基体与SiCp之间仍存在微裂纹,冶金结合状况差,材料抗拉强度和伸长率较低;当压下量大于45%时,沉积颗粒之间、Al基体与SiCp之间的微裂纹弥合,界面结合改善,压制过程中SiC-Al界面处无明显的Al4C3脆性相生成;压下量继续提高,复合材料显微组织无明显变化,但SiCp分布更均匀。当压下量为50%时,材料抗拉强度增加至291MPa,伸长率提高到2.4%。

关键词：铝基复合材料楔形压制显微组织力学性能

浅谈多车型保险杠防护套和存储器具的设计及应用

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汽车实用技术 2020年第17期45卷 179-181页

作者：张上殷刘天兵佟锁任晓宇张翰鑫浙江吉润春晓汽车部件有限公司浙江宁波315800

针对多车型保险杠混线生产,为了提升保险杠油漆件质量和转运效率,合理的防护材料以及高效的存储和转运方式需要得到充分的讨论和应用。通过开发新型保险杠防护套,改善保险杠的挤压和磕碰;通过优化存储器具和作业工艺,实现转运与存储功... 详细信息

针对多车型保险杠混线生产,为了提升保险杠油漆件质量和转运效率,合理的防护材料以及高效的存储和转运方式需要得到充分的讨论和应用。通过开发新型保险杠防护套,改善保险杠的挤压和磕碰;通过优化存储器具和作业工艺,实现转运与存储功能通用,减小库存面积和减少过程搬运频次;通过在库存区安置轨道,悬挂标识牌,转运存储器具时,实现机械防错,降低现场管理和确保了出入库先进先出管理要求。

关键词：保险杠产品存储转运油漆件防护转运效率多车型