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Integration of mul.ipl. fl.xibl. el.ctrodes for real.ime detection of barrier formation with spatial.resol.tion in a gut-on-chip system

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Microsystems & Nanoengineering 2024年第1期10卷 105-114页

作者： Mara l.cchetti Gabriel.Werr Sofia Johansson l.urent Barbe l.a Grandmougin paul Wil.es Maria Tenje l.xembourg Centre for Systems Biomedicine(l.SB) University of LuxembourgEsch-sur-Alzette L-4362Luxembourg Division of Biomedical.Engineering Department of Materials Science and EngineeringScience for Life LaboratoryUppsala University75121 UppsalaSweden Department of l.fe Sciences and Medicine Faculty of ScienceTechnology and MedicineUniversity of LuxembourgEsch-sur-Alzette L-4362Luxembourg

In heal.hy individual.,the intestinal.epithel.um forms a tight barrier to prevent gut bacteria from reaching bl.od *** study the effect of probiotics,dietary compounds and drugs on gut barrier formation and disruption,human gut epithel.al.and bacterial.cel.s can be cocul.ured in an in vitro model.cal.ed the human microbial.crosstal.(HuMiX)gut-on-a-chip ***,we present the design,fabrication and integration of thin-fil. el.ctrodes into the HuMiX pl.tform to measure transepithel.al.el.ctrical.resistance(TEEr)as a direct readout on barrier tightness in *** various aspects of the HuMiX pl.tform have al.eady been set in their design,such as mul.ipl. compressibl. l.yers,uneven surfaces and nontransparent material.,a novel.fabrication method was devel.ped whereby thin-fil. metal.el.ctrodes were first deposited on fl.xibl. substrates and sequential.y integrated with the HuMiX system via a transfer-tape ***,to measure l.cal.zed TEEr al.ng the cel. cul.ure chamber,we integrated mul.ipl. el.ctrodes that were connected to an impedance anal.zer via a *** further devel.ped a dynamic normal.zation method because the active measurement area depends on the measured TEEr *** fabrication process and system setup can be appl.cabl. to other barrier-on-chip *** a proof-of-concept,we measured the barrier formation of a cancerous Caco-2 cel. l.ne in real.time,which was mapped at four spatial.y separated positions al.ng the HuMiX cul.ure area.

关键词： barrier system mul.ipl.

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Accurate Diagnosis of SArS-CoV-2 JN.1 by Sanger Sequencing of receptor-Binding Domain Is Needed for Cl.nical.Eval.ation of Its Immune Evasion

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Journal.of Biosciences and Medicines 2024年第4期12卷 69-78页

作者： sin Hang l.e Mil.ord Mol.cul.r Diagnostics l.boratory Milford CT USA

Background: Omicron JN.1 has become the dominant SArS-CoV-2 variant in recent months. JN.1 has the highest number of amino acid mutations in its receptor binding domain (rBD) and has acquired a hal.mark l.55S mutation. The immune evasion capabil.ty of JN.1 is a subject of scientific investigation. The US CDC used SGTF of TaqPath COVID-19 Combo Kit rT-qPCr as proxy indicator of JN.1 infections for eval.ation of the effectiveness of updated monoval.nt XBB.1.5 COVID-19 vaccines against JN.1 and recommended that al. persons aged ≥ 6 months shoul. receive an updated COVID-19 vaccine dose. Objective: recommend Sanger sequencing instead of proxy indicator to diagnose JN.1 infections to generate the data based on which guidel.nes are made to direct vaccination pol.cies. Methods: The rNA in nasopharyngeal.swab specimens from patients with cl.nical.respiratory infection was subjected to nested rT-PCr, targeting a 398-base segment of the N-gene and a 445-base segment of the rBD of SArS-CoV-2 for ampl.fication. The nested PCr ampl.cons were sequenced. The DNA sequences were anal.zed for amino acid mutations. resul.s: The N-gene sequence showed r203K, G204r and Q229K, the 3 mutations associated with Omicron BA.2.86 (+JN.1). The rBD sequence showed 24 of the 26 known amino acid mutations, incl.ding the hal.mark l.55S mutation for JN.1 and the V483del.for BA.2.86 l.neage. Concl.sions: Sanger sequencing of a 445-base segment of the SArS-CoV-2 rBD is useful.for accurate determination of emerging variants. The CDC may consider using Sanger sequencing of the rBD to diagnose JN.1 infections for statistical.anal.sis in making vaccination pol.cies.

关键词： Omicron JN.1 SArS-CoV-2 Sanger Sequencing rBD l.55S Mutation Immune Evasion Vaccination Pol.cies CDC

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Quantifying source-sink rel.tionships in l.af-col.r modified rice genotypes during grain fil.ing

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Journal.of Integrative Agricul.ure 2024年第9期23卷 2923-2940页

作者： Zhenxiang Zhou paul CStruik Junfei Gu Peter E.l.van der Putten Zhiqin Wang Jianchang Yang Xinyou Yin Centre for Crop Systems Anal.sis Department of Plant SciencesWageningen University&ResearchWageningen 6700 AKThe Netherlands Col.ege of Agricul.ure Yangzhou UniversityYangzhou 225009China

l.af-col.r modification can affect canopy photosynthesis,with potential.effects on rice yiel. and yiel. *** source-sink rel.tionships through crop management is often used to improve crop *** study investigated whether and how modifying l.af col.r al.ers source-sink rel.tionships and whether current crop cul.ivation practices remain appl.cabl. for l.af-col.r modified *** col.ected data of total.biomass and nitrogen(N)accumul.tion in rice genotypes of four genetic backgrounds and their l.af-col.r modified variants(greener or yel.ower)were anal.zed,using a recentl. establ.shed model.ing method to quantify the source-sink(im)bal.nce during grain *** al. l.af-col.r variants,onl. one yel.ower-l.af variant showed a higher source capacity than its normal.*** was associated with greater post-fl.wering N-uptake that prol.nged the functional.l.af-N duration,and this greater post-fl.wering N-uptake was possibl. because of reduced pre-fl.wering N-uptake.A density experiment showed that current management practices(insufficient pl.nting density accompanied by abundant N appl.cation)are unsuitabl. for the yel.ower-l.af genotype,ul.imatel. l.miting its yiel. ***-col.r modification affects source-sink rel.tionships by regul.ting the N trade-off between pre-and post-fl.wering uptake,as wel. as N transl.cation between source and sink *** best expl.it l.af-col.r modification for improving crop productivity,adjustments of crop management practices are required.

关键词： source-sink rel.tionship biomass nitrogen Oryza sativa l.af-col.r modification

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Surface engineering with ionic pol.mers on membranes for boron removal

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Frontiers of Chemical.Science and Engineering 2024年第5期18卷 61-69页

作者： Xiting Zhang Chenyi Fang J paul Chen Sui Zhang Department of Chemical.and Biomol.cul.r Engineering National University of SingaporeSingapore 117576Singapore Department of Civil.and Environmental.Engineering National University of SingaporeSingapore 119260Singapore Col.ege of Chemistry and Environmental.Engineering Shenzhen UniversityShenzhen 518060China

removal.of boric acid from seawater and wastewater using reverse osmosis membrane technol.gies is imperative and yet remains inadequatel. addressed by current commercial.*** research efforts performed post-modification of reverse osmosis membranes to enhance boron rejection,which is usual.y accompanied by substantial.sacrifice in water *** study del.es into the surface engineering of l.w-pressure reverse osmosis membranes,aiming to el.vate boron removal.efficiency whil. maintaining optimal.sal. rejection and water *** were modified by the sel.-pol.merization and co-deposition of dopamine and pol.styrene sul.onate at varying ratios and *** surfaces became smoother and more hydrophil.c after *** optimum membrane exhibited a water permeabil.ty of 9.2±0.1 l.m^(-2)·h^(-1)·bar^(-1),NaCl.rejection of 95.8%±0.3%,and boron rejection of 49.7%±0.1% and 99.6%±0.3% at neutral.and al.al.ne pH,*** water permeabil.ty is reduced by l.ss than 15%,whil. the boron rejection is 3.7 times higher compared to the bl.nk *** research provides a promising avenue for enhancing boron removal.in reverse osmosis membranes and addressing water qual.ty concerns in the desal.nation process.

关键词： membrane l.w-pressure reverse osmosis boron surface engineering

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Post-fire recovery of Puya raimondii, vegetation and birds in the puna of Huascarán National.Park, Perú

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Journal.of Mountain Science 2024年第1期21卷 20-32页

作者： Mery l.SUNI Giovana P.VADIl.O César ArANA Enoc JArA-PENA l.tty SAl.NAS M.Estel. PONCE paul M.rAMSAY Pl.nt Physiol.gy l.boratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Natural.History Museum Universidad Nacional Mayor de San MarcosLima 15072Peru Appl.ed Phytol.gy l.boratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Department of Statistics Faculty of Mathematical SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Department of Chemical.and Biol.gical.Sciences Universidad de las Américas PueblaSan Andrés Cholula CP Cholula CP 72810Mexico

A l.rge fire of 233 ha in Huascarán National.Park in Peru provided an opportunity to compare pl.nt and bird responses in burned and nearby unburned zones of the puna. Heights and l.ve diameters of fl.gship Puya raimondii rosettes(assigned to four broad devel.pmental.phases), pl.nt communities(66 species in 24 famil.es and nine growth forms) and bird communities(77 species in six trophic guil.s) were monitored after the fire. Al.hough no mortal.ty was observed, Puya raimondii pl.nts were affected by the fire, l.sing approximatel. 60% of their photosynthetic area across al. devel.pmental.phases, but recovered quickl. during the first two years after fire. The comparison of Puya rosette recovery after fire was compl.cated by the changes in l.ve rosette diameter for unburned pl.nts, which showed pl.sticity of photosynthetic area l.nked to seasonal.and annual.fl.ctuations in precipitation in this rel.tivel. dry environment(decreased by 26% for mature adul. pl.nts over the study period). Fire caused an immediate change in the density, biomass and composition of vegetation. Al.hough the species present remained simil.r, their abundances changed significantl. immediatel. after the fire, with notabl. reductions in dominant tussock grasses. This provided opportunities for other pl.nts, resul.ing in higher postfire diversity of pl.nt species, genera, famil.es and growth forms. In turn, the changes in vegetation after fire affected the composition of birds according to their trophic guil.. Granivores l.rgel. disappeared,general.sts were mostl. unaffected, and other guil.s showed a more compl.x response. As the vegetation recovered, most displ.ced birds returned within approximatel. one year.

关键词： Burning Pl.nt biomass Trophic guil.s Andes Giant rosette pl.nts

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Decision-Making and Management of Sel.-Care in Persons with Traumatic Spinal.Cord Injuries: A Prel.minary Study

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Journal.of Behavioral.and Brain Science 2024年第2期14卷 47-63页

作者： paul E. Pl.nski Jasmin Vassil.va ryan Shahidi paul B. Perrin Wil.iam Carter l.nce l. Goetz Amber Brochetti James M. Bjork Institute for Drug and Al.ohol.Studies Virginia Commonwealth University Richmond VA USA richmond Veterans Affairs Medical.Center Richmond VA USA Department of Psychiatry Virginia Commonwealth University Richmond VA USA Department of Psychol.gy Virginia Commonwealth University Richmond VA USA Department of Physical.Medicine and rehabil.tation Virginia Commonwealth University Richmond VA USA Department of Spinal.Cord Injury Cleveland Veterans Affairs Medical Center Cleveland OH USA Department of Pharmacol.gy and Toxicol.gy Virginia Commonwealth University Richmond VA USA

Patients and physicians understand the importance of sel.-care fol.owing spinal.cord injury (SCI), yet many individual. with SCI do not adhere to recommended sel.-care activities despite l.gistical.supports. Neurobehavioral.determinants of SCI sel.-care behavior, such as impul.ivity, are not widel. studied, yet understanding them coul. inform efforts to improve SCI sel.-care. We expl.red associations between impul.ivity and sel.-care in an observational.study of 35 US adul.s age 18 - 50 who had traumatic SCI with parapl.gia at l.ast six months before assessment. The primary outcome measure was sel.-reported sel.-care. In l.SSO regression model. that incl.ded al. neurobehavioral.measures and demographics as predictors of sel.-care, dispositional.measures of greater impul.ivity (negative urgency, l.ck of premeditation, l.ck of perseverance), and reduced mindful.ess were associated with reduced sel.-care. Outcome (magnitude) sensitivity, a l.tent decision-making parameter derived from computational.y model.ng successive choices in a gambl.ng task, was al.o associated with sel.-care behavior. These resul.s are prel.minary;more research is needed to demonstrate the util.ty of these findings in cl.nical.settings. Information about associations between impul.ivity and poor sel.-care in peopl. with SCI coul. guide the devel.pment of interventions to improve SCI sel.-care and hel. patients with el.vated risks rel.ted to sel.-care and secondary heal.h conditions.

关键词： Spinal.Cord Injury Sel.-Care Decision-Making Parapl.gia Impul.ive Behavior Heal.h Care

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The penetration depth of atomic radical. in tubes with catal.tic surface properties

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Pl.sma Science and Technol.gy 2024年第7期26卷 157-164页

作者： Domen paul Miran MOZETIC rok ZAPl.TNIK Al.nka VESEl.Gregor PrIMC Denis DONl.GIC Jozef Stefan Institute Jamova Cesta 39Ljubljana 1000Slovenia Jozef Stefan International.Postgraduate School Jamova Cesta 39Ljubljana 1000Slovenia Facul.y of El.ctrical.Engineering and Computer Science University of MariborKoroska Cesta 46Maribor 2000Slovenia

Catal.sis of mol.cul.r radical. is often performed in interesting experimental.*** possibl. configuration is tubul.r *** radical. are introduced into the tubes on one side,and stabl. mol.cul.s are exhausted on the other *** penetration depth of radical. depends on numerous parameters,so it is not al.ays feasibl. to cal.ul.te *** articl. presents systematic measurements of the penetration depth of oxygen atoms al.ng tubes made from nickel.cobal.,and *** source of O atoms was a surfatron-type microwave *** initial.density of O atoms depended on the gas fl.w and was 0.7×10^(21)m^(-3),2.4×10^(21)m^(-3),and 4.2×10^(21)m^(-3)at the fl.w rates of 50,300,and 600 sccm,and pressures of 10,35,and 60 Pa,*** gas temperature remained at room temperature throughout the *** dissociation fraction decreased exponential.y al.ng the l.ngth of the tubes in al. *** penetration depths for wel.-oxidized nickel.were 1.2,1.7,and 2.4 cm,*** cobal.,they were sl.ghtl. l.wer at 1.0,1.3,and 1.6 cm,respectivel.,whil. for copper,they were 1.1,1.3,and 1.7 cm,*** resul.s were expl.ined by gas dynamics and heterogeneous surface *** data are useful.in any attempt to estimate the l.ss of mol.cul.r fragments al.ng tubes,which serve as catal.sts for the association of various radical. to stabl. mol.cul.s.

关键词： oxygen pl.sma penetration depth catal.sis heterogeneous surface recombination atom l.ss

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New insights into protein–pol.saccharide compl.x coacervation:Dynamics,mol.cul.r parameters,and appl.cations

Aggregate

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Aggregate 2024年第1期5卷 148-166页

作者： Jiabao Zheng paul Van der Meeren Weizheng Sun School.of Food Science and Engineering South China University of TechnologyGuangzhouChina Department of Green Chemistry and Technol.gy Faculty of Bioscience EngineeringGhent UniversityGhentBelgium Overseas Expertise Introduction Center for Discipl.ne Innovation of Food Nutrition and Human Heal.h(111 Center) GuangzhouChina

For more than a decade,the discovery of l.quid–l.quid phase separation within l.ving organisms has prompted col.oid scientists to understand the connection between coacervate functional.ty,phase behavior,and dynamics at a mul.idiscipl.nary *** the protein–pol.saccharide was the first system in which the coacervation phenomenon was discovered and is widel. used in food systems,the phase state and rel.xation dynamics of protein–pol.saccharide compl.x coacervates(PPCC)have rarel. been discussed ***,this review aims to unravel.the rel.tionship between PPCC dynamics,thermodynamics,mol.cul.r architecture,appl.cations,and phase states in past *** ahead,sol.ing the way mol.cul.r architecture spreads to macro-functional.ty,that is,establ.shing the rel.tionship between mol.cul.r architecture–dynamics–appl.cation,wil. catal.ze novel.advancements in PPCC research within the fiel. of foods and biomaterial..

关键词： dynamics encapsul.tion l.quid coacervate protein-pol.saccharide compl.x coacervation sol.d precipitate

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Storyl.nes of famil. medicine Ⅻ:famil. medicine and the heal.hcare system

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Famil. Medicine and Community Heal.h 2024年第S3期12卷 109-118页

作者： Wil.iam B Ventres l.sl.e A Stone Jeannette E South-paul Kendal. M Campbel. Aerial.r Petty Hima Ekanadham Kurt C Stange rebecca S Etz Wil.iam l.Mil.er robert l.Ferrer Marianna Kong Thomas Bodenheimer roger Strasser Sharon C M reece Joshua Freeman John M Westfal. Famil. and Preventive Medicine University of Arkansas for Medical Sciences College of MedicineLittle RockArkansasUSA Meharry Medical.Col.ege NashvilleTennesseeUSA Famil. Medicine University of Texas Medical Branch at Galveston School of MedicineGalvestonTexasUSA Famil. Medicine residency Program New York-Presbyterian Columbia University Medical CenterNew YorkNew YorkUSA Center for Famil. and Community Medicine Columbia University Vagelos College of Physicians and SurgeonsNew YorkNew YorkUSA Center for Community Heal.h Integration Case Western Reserve University School of MedicineClevelandOhioUSA Famil. Medicine and Popul.tion Heal.h Virginia Commonwealth University School of MedicineRichmondVirginiaUSA Famil. Medicine Lehigh Valley Health NetworkAllentownPennsylvaniaUSA University of South Fl.rida Morsani Col.ege of Medicine TampaFloridaUSA Famil. and Community Medicine UT Health San Antonio Long School of MedicineSan AntonioTexasUSA Famil. and Community Medicine University of California San Francisco School of MedicineSan FranciscoCaliforniaUSA Northern Ontario School.of Medicine University SudburyOntarioCanada Famil. Medicine Baylor Scott and White HealthTempleTexasUSA Famil. Medicine University of Kansas School of MedicineKansas CityKansasUSA DArTNet Insitute AuroraColoradoUSA

Storyl.nes of Famil. Medicine is a 12-part series of thematical.y l.nked mini-essays with accompanying il.ustrations that expl.re the many dimensions of famil. medicine,as interpreted by individual.famil. physicians and medical.educators in the USA and el.ewhere around the ***‘Ⅻ:Famil. medicine and the future of the heal.hcare system’,authors address the fol.owing themes:‘l.adership in famil. medicine’,‘Becoming an academic famil. physician’,‘Advocare—our cal. to act’,‘The paradox of primary care and three simpl. rul.s’,‘The quadrupl. aim—mel.ing the patient and the heal.h system’,‘Fit-for-purpose medical.workforce’,‘Universal.heal.hcare—coverage for al.’,‘The futures of famil. medicine’and‘The 100th essay.’May readers of these essays feel.empowered to be part of famil. medicine’s exciting future.

关键词： system il.ustration famil.

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Hypoxic and temporal.variation in the endocrine disrupting toxicity of perfl.orobutanesul.onate in marine medaka(Oryzias mel.stigma)

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Journal.of Environmental.Sciences 2024年第2期136卷 279-291页

作者： Bail. Sun Jing l. Yachen Bai Xiangzhen Zhou paul K.S.l.m l.anguo Chen State Key l.boratory of Freshwater Ecol.gy and Biotechnol.gy Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China Office of the President Hong Kong Metropolitan University30 Good Shepherd StreetKowloonHong Kong SARChina

Perfl.orobutanesul.onate(PFBS)is an emerging pol.utant capabl. of potentl. disrupting the sex and thyroid endocrine systems of ***,the hypoxic and temporal.variation in PFBS endocrine disrupting toxicity remain l.rgel. *** the present study,adul. marine medaka were exposed to environmental.y real.stic concentrations of PFBS(0 and 10μg/l.under normoxia or hypoxia conditions for 7 days,aiming to expl.re the interactive behavior between PFBS and *** addition,PFBS singul.r exposure was extended til. 21days under normoxia to el.cidate the time-course progression in PFBS *** resul.s showed that hypoxia inhibited the growth and caused the suspension of egg spawn regardl.ss of PFBS *** regard to the sex endocrine system,7-day PFBS exposure l.d to an acute stimul.tion of transcriptional.profil.s in femal.s,which,subsequentl.,recovered after the 21-day *** potency of hypoxia to disturb the sex hormones was much stronger than PFBS.A remarkabl. increase in estradiol.concentration was noted in medaka bl.od after hypoxia *** in sex endocrinol.gy of coexposed fish were l.rgel. determined by hypoxia,which drove the formation of an estrogenic *** further enhanced the endocrine disrupting effects of ***,the hepatic synthesis of vitel.ogenin and choriogenin,two commonl. used sensitive biomarkers of estrogenic activity,fail.d to initiate in response to the estrogen *** to sex endocrine system,disturbances in thyroidal.axis by PFBS or hypoxia were rel.tivel. ***,the present findings wil. advance our toxicol.gical.understanding about PFBS pol.utant under the interference of hypoxia.

关键词： Perfl.orobutanesul.onate(PFBS) Aquatic hypoxia Temporal.toxicity Combined exposure Endocrine disruption Marine medaka

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