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Journal of Central South University 2017年第6期24卷 1443-1449页

作者： ZHANG He-hui DENG Sheng-xiang qu ying-jie School of Energy Science and Engineering Central South UniversityChangsha 410083China Hunan M&W Energy Saving Tech.Co.Ltd. Changsha 410208China

The three-dimensional internal flow field of centrifugal pump is complex and variable with design parameters and operation conditions. The post-processing technique named differential amplification method was proposed for the comparison study of different flow structures. The full steady flow fields of an industrial centrifugal pump working on-design and off-design points were numerically investigated by solving Reynolds average Navier-Stokes equations together with a shear-stress transport(SST) k-? turbulence model. And the numerically predicted performance curves of the studied pump agree well with test measurement results. Compared with the flow flied on design point under the help of differential amplification method, the disturbance caused by interaction between blade and volute tongue is very obvious and it extends to the diffuser pipe on the working point with 0.8 times rated flux. While on the point with 1.2 times rated flux, the flow distribution in impeller region is roughly even and it flows more to the bottom section of the diffuser pipe. The above method was proved to be good at displaying the subtle secondary flow structure changes with a higher resolution effect relative to single isolated case observation, which helps the optimization decision-making from multiple design cases.

关键词： differential amplification computational fluid dynamics (CFD) flow structure centrifugal pump

Novel matrine derivative MD-1 attenuates hepatic fibrosis by inhibiting EGFR activation of hepatic stellate cells

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Protein & Cell 2016年第9期7卷 662-672页

作者： Yi Feng Hai-yan ying ying qu Xiao-bo Cai Ming-yi Xu Lun-gen Lu Department of Gastroenterology Shanghai General Hospital Nanjing Medical University Shanghai 200080 China

Matrine （MT）, the effective component of Sophora fla- vescens Air, has been shown to have anti-inflammation, immune-suppressive, anti-tumor, and anti-hepatic fibrosis activities. However, the pharmacological effects of MT still need to be strengthened due to its relatively low efficacy and short half-life. In the present study, we report a more effective thio derivative of MT, MD-1, and its inhibitory effects on the activation of hepatic stellate cells （HSCs） in both cell culture and animal models. Cytological experiments showed that MD-1 can inhibit the proliferation of HSC-T6 cells with a half-maximal inhibitory concentration （ICs0） of 62 pmollL. In addition, MD-1 more strongly inhibits the migration of HSC-T6 cells compared to MT and can more effectively induce G0/G1 arrest and apoptosis. Investigating the biological mechanisms underlying anti-hepatic fibrosis in the presence of MD-I, we found that MD-I can bind the epidermal growth factor receptor （EGFR） on the surface of HSC-T6 cells, which can further inhibit the phospho- rylaUon of EGFR and its downstream protein kinase B （Akt）, resulting in decreased expression of cyclin D1 and eventual inhibition of the activation of HSC-T6 cells. Furthermore, in rats with dimethylnitrosamine （DMN）- induced hepatic fibrosis, MD-1 slowed the development and progression of hepatic fibrosis, protecting hepatic parenchymal cells and improving hepatic functions. Therefore, MD-1 is a potential drug for anti-hepatic fibrosis.

关键词： matrine derivative hepatic stellate cellhepatic fibrosis epidermal growth factor receptor signaltransduction pathway

Application of nanotechnology in CAR-T-cell immunotherapy

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Chinese Chemical Letters 2023年第3期34卷 49-65页

作者： Qiang Zeng Zhigang Liu Ting Niu Chuan He ying qu Zhiyong Qian Department of Hematology and Institute of Hematology State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

It is cellular immunotherapy for the tumor that the in vitro modified immunocytes from patients or donors are reinfused into patients to kill tumor *** antigen receptor T cell(CAR-T)therapy,one of the most successful and representative tumor cellular immunotherapies,is now the weapon for cancer after extensive *** CAR-T immunotherapy achieves success in treating relapsed/refractory hematological tumors,its drawbacks,including the poor effect in solid tumors,cytokine release syndrome(CRS)or CAR-T-related encephalopathy syndrome(CRES),on-target,off-tumor effect,and high cost,cannot be *** is advantageous in the construction of CARs,the transfection of T cells,the expansion,delivery,and antitumor effect of CAR-T cells,and the reduction of CAR-T therapy-associated ***,introducing nanotechnology into CAR-T immunotherapy has already been performed in numerous studies with highly promising *** this review,we summarized the nanotechnologies used in CAR-T immunotherapy and discussed the challenges and directions of CAR-T immunotherapy combined with nanotechnologies in the future.

关键词： CAR-T immunotherapy Nanotechnology Nanobody Nanoparticle

Inhibitory effect of Polo-like kinase 1 depletion on mitosis and apoptosis of gastric cancer cells

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World Journal of Gastroenterology 2006年第1期12卷 29-35页

作者： Xue-Hua Chen Bin Lan ying qu xiao-Qing Zhang qu Cai Bing-Ya Liu Zheng-Gang Zhu Department of Surgery Shanghai Institute ofDigestive Surgery Ruijin Hospital Medical School of ShanghaiJiao Tong University Shanghai 200025 China Department of Surgical Oncology the First AffiliatedHospital Fujian Medical University Fuzhou 350005 FujianProvince China

AIM： Polo-like kinase 1 （PLK1） serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cancer cells. METHODS： PLK1 expression was blocked by small RNA interference（siRNA）. The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Then, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitotic spindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed. RESULTS： PLK1 gene knockdown was associated with increased cyclin B expression, increased cdc2 activity （but not with the expression levels）, accumulation of gastric cancer cells at G2/M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis. CONCLUSION： Blockage of to decreased mitosis or even PLK1 expression may lead apoptosis in gastric cancer cells, indicating that PLK1 may be a valuable therapeutic target for gastric cancer.

关键词： Gastric cancer cells PLK1 gene Mitosis Cell cycle Apoptosis

Influence of physical self and mindfulness on the academic possible selves of freshman nursing students

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Frontiers of Nursing 2019年第3期6卷 241-248页

作者： Mei-Ling Han Hai-ying qu School of Humanities and Social Sciences Binzhou Medical University Yantai

Objective:The purposes of this study were to investigate the general level of the mindfulness,physical self,and academic possible selves in the freshman nursing students,and to explore the relationship among the three variables,so as to provide a theoretical basis for the freshmen to improve their academic achievement on the basis of the correct understanding of the physical ***:A total of 360 freshman nursing students were enrolled in this study from a medical school in Yantai *** were collected using three questionnaires:Body Self-description questionnaire,Five Factors Mindfulness questionnaire,and Academic Possible Selves *** were 341 valid ***:The mindfulness of the freshmen nursing students was at a medium level(3.03±0.28),their attitude to physical self(3.66±0.58)and academic possible selves(3.33±0.50)were *** were significant gender differences among coordination,physical activity,athletic ability,strength,endurance,observation,non-response,total physical self-score,and negative future academic possible *** the above factors,except negative future academic possible selves,the scores for boys were higher than that for girls(all P<0.05).There were significant positive relationships between physical self,mindfulness,and academic possible ***-esteem,acting with awareness,endurance,and description were effective positive predictors of the academic possible selves,with a total explanation rate of 33.6%.Conclusion:There were close relationships among the physical self,the level of mindfulness,and the academic possible *** physical self-description and high level of mindfulness could help individuals develop a positive attitude toward their academic possible selves.

关键词： freshman nursing students physical self mindfulness academic possible selves medical school

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肺动脉高压孕产妇病因及妊娠结局分析

局解手术学杂志 2024年第8期33卷 675-679页

作者：张程程张金慧曲冬颖北部战区总医院妇产科辽宁沈阳110003 仓乐县人民医院妇产科山东潍坊262400

目的探究妊娠合并肺动脉高压的原发病因构成,分析患者的妊娠结局及其相关影响因素,为指导该类患者孕期管理提供新的循证医学数据。方法选取于北部战区总医院终止妊娠的肺动脉高压孕产妇57例,根据患者肺动脉高压严重程度将其分为轻度组(2... 详细信息

目的探究妊娠合并肺动脉高压的原发病因构成,分析患者的妊娠结局及其相关影响因素,为指导该类患者孕期管理提供新的循证医学数据。方法选取于北部战区总医院终止妊娠的肺动脉高压孕产妇57例,根据患者肺动脉高压严重程度将其分为轻度组(26例)、中度组(20例)及重度组(11例),选择同期肺动脉压力正常的孕产妇138例作为对照组。收集并比较各组孕产妇的临床资料,分析肺动脉高压原发病因构成、不同严重程度肺动脉高压患者的妊娠结局及其相关影响因素。结果先天性心脏病(CHD)是肺动脉高压孕产妇的主要原发病因,占63.2%。不同组别复杂型CHD患者比例比较,差异有统计学意义(P<0.05)。重度组心功能Ⅲ~Ⅳ级的患者比例高于对照组和轻、中度组,差异有统计学意义(P<0.05);重度组心电图异常的患者比例高于轻度组和对照组,差异有统计学意义(P<0.05)。对照组和轻度组的足月活婴分娩率高于中、重度组,差异有明显统计学意义(P<0.05);重度组死胎率高于其他组,差异有统计学意义(P<0.05);对照组和轻度组新生儿窒息率低于中、重度组,差异有统计学意义(P<0.05);对照组及轻度组新生儿出生体质量高于中、重度组,差异有统计学意义(P<0.05)。结论妊娠合并肺动脉高压最常见的原发病因是CHD。轻、中度肺动脉高压孕产妇出现不良妊娠结局的风险较重度肺动脉高压孕产妇低。可利用多普勒二维超声评估、孕产妇肺动脉压力监测及时给予干预,改善妊娠及胎儿结局。

关键词：妊娠肺动脉高压病因妊娠结局

A novel injectable fibroblasts-porous PDLLA microspheres/collagen gel composite for soft-tissue augmentation

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Progress in Natural Science:Materials International 2020年第5期30卷 651-660页

作者： Qiuxia Ding Ruoyu Zha Xinye He Ao Sun Kun Shi ying qu Zhiyong Qian State Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University Urology Institute of Shenzhen University The Third Affiliated Hospital of Shenzhen University Shenzhen University

This study designed a novel porous PDLLA microspheres/collagen gel composite combined with fibroblasts as the injectable dermal filler for soft-tissue augmentation. The low degradation rate of porous PDLLA microspheres and seed cells were introduced in the system to achieve the filling volume stability and stronger regeneration activity. Biodegradable PDLLA porous microspheres were prepared by an improved water-in-oil-in-water double emulsion solvent evaporation method. The combine of fibroblasts with porous microspheres was observed through co-culture of cells with microspheres, SEM(scanning electron microscope) and fluorescent staining technology. The MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the hemolysis test and histocompatibility experiment indicated that the composite had satisfactory biocompatibility in vitro and in vivo. In the animal studies, the composite was implanted into the subcutaneous part of the rats’ back through sterile needles. The collagen was metabolized within 30 days, meanwhile, fibroblasts-porous PDLLA microspheres served as scaffold structure and seed cells which promote new connective tissue formation. The histopathological studies demonstrate that the fibroblasts-porous PDLLA microspheres/collagen gel composite group could create larger amount of supportive structure than PDLLA porous microspheres/collagen gel composite group. All the results indicate that fibroblasts-porous PDLLA microspheres/collagen gel composite may have great clinical application in soft-tissue augmentation with its excellent biocompatibility, regeneration activity and stable long-term filling ability.

关键词： Porous PDLLA microspheres Fibroblast Collagen Soft-tissue augmentation

A survey of the influencing factors and models for resident＇s household waste management behavior

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Ecological Economy 2007年第3期3卷 336-344页

作者： qu ying Zhu Qinghua Murray Haight School of Management Dalian University of TechnologyDalian 116023.P.R.China School of Planning Faculty of Environmental Studies.University of Waterloo.Waterloo.Canada

The problem of household solid waste has been concerned and researched on by municipalities and *** present, household solid waste has been changed to management problem from technical one. From the point view ofmanagement, the research on household solid waste is to study the factors which influence resident’s behavior ofmanaging their waste. Based on the literature review, firstly, this paper summarizes those factors which have alreadybeen identified to have impact on resident’s behavior of managing their waste. They are social-demographic variables,knowledge, environmental values, psychological factors, publicity and system design. Secondly, three typical models ofthe relationship between factors and behavior, which are factors determining task performance in waste management,conceptualization of waste management behavior and the theoretical model of repeated behavior on household wastemanagement, are analyzed and the deficiencies of these models are also analyzed. Finally, according to the currentsituation in household waste management and the culture and resident’s habits in China, this paper puts forward aresearch focus and suggestions about resident’s behavior of household solid waste management.

关键词： Influential factors Model Behavior Household waste management

应用计算流体动力学方法估测医院急诊大厅颗粒物分布特征的研究

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环境卫生学杂志 2024年第5期14卷 384-392页

作者：张雪艳贾宁曲颖徐擎田昊渊周晓滢孙新中国疾病预防控制中心职业卫生与中毒控制所北京100050

目的基于室内场所的空间几何构型、风速、颗粒物浓度和环境温度测量值,阐述空气中颗粒物浓度、悬浮时间等分布规律。方法现场测量医院急诊大厅的空间及其中大型设施的尺寸,测量环境中颗粒物数量浓度、温度、风速,场所空间尺寸用于构建模... 详细信息

目的基于室内场所的空间几何构型、风速、颗粒物浓度和环境温度测量值,阐述空气中颗粒物浓度、悬浮时间等分布规律。方法现场测量医院急诊大厅的空间及其中大型设施的尺寸,测量环境中颗粒物数量浓度、温度、风速,场所空间尺寸用于构建模型,环境检测参数用于确定模型的边界条件和验证模型的正确性。基于格子波尔曼方法(Lattice Boltzmann Method,LBM)计算颗粒物运动,应用对流扩散方程计算空调送风系统出风口处颗粒物浓度的变化,离散相模型(discrete phase model,DPM)用于计算颗粒物在流场的运动情况。结果环境检测表明空调送风系统出口风速与室内颗粒物数量浓度呈负相关,人群聚集的候诊区颗粒物数量浓度高于诊室(P值均<0.05)。通过建立的数学模型进行仿真计算,仿真相对浓度未超过实测相对浓度的x±s范围,候诊人群使局部颗粒物浓度升高,候诊区人员咳嗽呼出气中的颗粒物可以在15 s内传播给周围的人,呼出气中60%的颗粒物悬浮时间可持续几分钟或更长时间。结论改变模型中的空间几何尺寸和环境检测参数可以用于计算不同室内场所中颗粒物的分布情况,仿真结果与实测结果具有可比性,数学计算结果可支持环境呼吸危害防控管理措施的制定。

关键词：颗粒物分布医院仿真计算流体动力学

Biomineralized polymer matrix composites for bone tissue repair:a review

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Science China Chemistry 2018年第12期61卷 1553-1567页

作者： Lin Zhong ying qu Kun Shi Bingyang Chu Minyi Lei Kangkang Huang yingchun Gu Zhiyong Qian State Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University Department of Textile Engineering College of Light Industry & Textile & Food Engineering Sichuan University

Bone defects caused by trauma, infection or bone tumor resection, are highly prevalent. A small number(5%–10%) of these injuries fail to heal due to non-union and require surgical intervention. Currently, the principal treatment options for these defects are autografts, allografts, xenografts or synthetic grafts. The main problems associated with these therapies include pain,infection and donor site morbidity. Bone tissue engineering is a diverse field that focuses on the regeneration of bone by combining cells, scaffolds, growth factors and dynamic forces. There have been many recent studies utilizing biomineralized polymer matrix composites which mimic the natural structure of bone. The principal focus of this review is on recent advances in the synthesis of various types of biomineralized polymer matrix composite. Examples of the biomineralization of naturallyderived and synthetic polymers widely used for bone engineering are also summarized.

关键词： bomineralization natural-based materials synthetic polymers bone engineering