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Warming intensified the effects of nitrogen addition on N_(2)O emissions from alpine meadow in the northern qinghai-Tibet Plateau

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Advances in Climate Change Research 2024年第1期15卷 101-112页

作者： Ming-Jie LI Yi-qing GE Hasbagan GANJURJAV Guo-Zheng HU Hong-Bao WU Jun YAN Shi-Cheng HE qing-Zhu gao Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081China National Agricultural Experimental Station for Agricultural Environment Nagqu 852100China Ecology and Nature Conservation Institute Chinese Academy of ForestryBeijing 100091China College of Resource and Environment Anhui Science and Technology UniversityFengyang 233100China Nagqu Agriculture Animal Husbandryand Prataculture Science and Technology Research and Extension CenterNagqu 852100China Tongliao Experimental Middle School Tongliao 028000China

Warming and nitrogen(N)addition may impact soil nitrous oxide(N_(2)O)emissions,but the relationship between plant community composition and soil microbial activities remains *** a two-year field study in the qinghai-Tibet Plateau,open-top chambers were used to quantify the effects of warming,N-addition,and their interactions on N_(2)O *** found that the N-addition greatly increased N_(2)O emissions by 77.4%in 2018 when compared to the control *** contrast,warming showed little effect on N_(2)0 emissions but did increase the activity of enzymes associated with soil nitrification and denitrification.A combined effect of warming and N-addition of resulted in 208.6%(2018)and 90.8%(2019)increase in N_(2)0 emissions,respectively,compared to the individual treatments of warming or *** warming in alpine meadows is causally linked to increased legume biomass which is further intensified with the *** legume biomass(p<0.05),soil moisture(p<0.001)and enzyme activity(p<0.001)had a positive effect on N_(2)0 emissions,while diminished microbial carbon/nitrogen(MBC/MBN)(p<0.05)correlated with reduced N_(2)O *** results indicated that N-addition has a positive effect on N_(2)O emissions,and the addition of warming further intensifies this *** increased dominance of legumes and microbial N content contributes to this *** outcomes suggest that warming and atmospheric N deposition can stimulate N_(2)O emissions of alpine meadows inthefuture.

关键词： Alpine meadow Community composition Nitrogen addition Nitrous oxide flux qinghai-Tibet Plateau Warming

Removal of microcystin-LR from drinking water using a bamboo-based charcoal adsorbent modified with chitosan

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Journal of Environmental Sciences 2011年第12期23卷 1983-1988页

作者： Hangjun Zhang Guoying Zhu Xiuying Jia Ying Ding Mi Zhang qing gao Ciming Hu Shuying Xu College of Biology and Environmental Sciences Hangzhou Normal University Hangzhou 310036 China Institute of Industry EP＆D Hangzhou 310005 China College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 China

A new kind of low-cost syntactic adsorbent from bamboo charcoal and chitosan was developed for the removal of microcystin-LR from drinking water. Removal effciency was higher for the syntactic adsorbent when the amount of bamboo charcoal was increased. The optimum dose ratio of bamboo charcoal to chitosan was 6：4, and the optimum amount was 15 mg/L; equilibrium time was 6 hr. The adsorption isotherm was non-linear and could be simulated by the Freundlich model （R2 = 0.9337）. Adsorption efficiency was strongly afflected by pH and natural organic matter （NOM）. Removal efficiency was 16% higher at pH 3 than at pH 9. Efficiency rate was reduced by 15% with 25 mg/L NOM （UV254 = 0.089 cm-1） in drinking water. This study demonstrated that the bamboo charcoal modified with chitosan can effectively remove microcystin-LR from drinking water.

关键词： adsorption bamboo charcoal chitosan microcystin-LR

High-confidence software evolution

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Science China(Information Sciences) 2016年第7期59卷 94-112页

作者： qing gao Jun LI Yingfei XIONG Dan HAO Xusheng XIAO Kunal TANEJA Lu ZHANG Tao XIE Key Laboratory of High Confidence Software Technologies Mo E Institute of Software School of Electronics Engineering and Computer Science Peking University NEC Laboratories America Inc. 3. Accenture Technology Labs 4. Department of Computer Science University of Illinois at Urbana-Champaign

Software continues to evolve due to changing requirements, platforms and other environmental pressures. Modern software is dependent on frameworks, and if the frameworks evolve, the software has to evolve as well. On the other hand, the software may be changed due to changing requirements. Therefore, in high-confidence software evolution, we must consider both framework evolution and client evolution, each of which may incur faults and reduce software quality. In this article, we present a set of approaches to address some problems in high-confidence software evolution. In particular, to support framework evolution, we propose a history-based matching approach to identify a set of transformation rules between different APIs, and a transformation language to support automatic transformation. To support client evolution for high-confidence software, we propose a path-exploration-based approach to generate tests efficiently by pruning paths irrelevant to changes between versions, several coverage-based approaches to optimize test execution, and approaches to locate faults and fix memory leaks automatically. These approaches facilitate high-confidence software evolution from various aspects.

关键词： software evolution high confidence software quality software development program analysis

Identification of a novel splice site mutation in the DNAAF4 gene of a Chinese patient with primary ciliary dyskinesia

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Asian Journal of Andrology 2023年第6期25卷 713-718页

作者： Yang Xu Jing Wang Ji-Hai Liu qing-Qiang gao Bing Wang Zhi-Peng Xu Department of Andrology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China

Primary ciliary dyskinesia(PCD)is a rare hereditary orphan condition that results in variable phenotypes,including *** 50 gene variants are reported in the scientific literature to cause PCD,and among them,dynein axonemal assembly factor 4(DNAAF4)has been recently ***4 has been implicated in the preassembly of a multiunit dynein protein essential for the normal function of locomotory cilia as well as *** the current study,a single patient belonging to a Chinese family was recruited,having been diagnosed with PCD and *** affected individual was a 32-year-old male from a nonconsanguineous *** also had abnormal spine structure and spinal cord bends at angles diagnosed with *** reports,laboratory results,and imaging data were ***-exome sequencing,Sanger sequencing,immunofluorescence analysis,hematoxylin-eosin staining,and in silico functional analysis,including protein modeling and docking studies,were *** results identified DNAAF4 disease-related variants and confirmed their *** analysis through whole-exome sequencing identified two pathogenic biallelic variants in the affected *** identified variants were a hemizygous splice site c.784-1G>A and heterozygous 20.1 Kb deletion at the DNAAF4 locus,resulting in a truncated and functionless DNAAF4 *** analysis indicated that the inner dynein arm was not present in the sperm flagellum,and sperm morphological analysis revealed small sperm with twisted and curved flagella or lacking *** current study found novel biallelic variants causing PCD and asthenoteratozoospermia,extending the range of DNAAF4 pathogenic variants in PCD and associated with the etiology of *** findings will improve our understanding of the etiology of PCD.

关键词： DNAAF primary ciliary dyskinesia scoliosis splice site

Effect of particle shape on packing fraction and velocity profiles at outlet of a silo

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Chinese Physics B 2023年第6期32卷 368-377页

作者：高庆庆陈玉超胡林 College of Physics Guizhou UniversityGuiyang 550025China Department of Civil Engineering Nanyang Normal UniversityNanyang 473061China

Many studies on how the particle shape affects the discharge flow mainly focus on discharge rates and avalanche statistics. In this study, the effect of the particle shape on the packing fraction and velocities of particles in the silo discharge flow are investigated by using the discrete element method. The time-averaged packing fraction and velocity profiles through the aperture are systematically measured for superelliptical particles with different blockinesses. Increasing the particle blockiness is found to increase resistance to flow and reduce the flow rate. At an identical outlet size, larger particle blockiness leads to lower velocity and packing fraction at the outlet. The packing fraction profiles display evidently the self-similar feature that can be appropriately adjusted by fractional power law. The velocity profiles for particles with different shapes obey a uniform self-similar law that is in accord with previous experimental results, which is compatible with the hypothesis of free fall arch. To further investigate the origin of flow behaviors, the packing fraction and velocity field in the region above the orifice are computed. Based on these observations, the flow rate of superelliptical particles is calculated and in agreement with the simulated data.

关键词： superelliptical particles flow rate packing fraction and velocity profiles discrete element method

Efficacy and Safety of Guihuang Formula in Treating Type Ⅲ Prostatitis Patients with Dampness-Heat and Blood Stasis Syndrome: A Randomized Controlled Trial

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Chinese Journal of Integrative Medicine 2022年第10期28卷 879-884页

作者： LIU Sheng-jing DENG Ying-jun ZENG Yin ZHAO Ming GUO Jun gao qing-he Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing(100091)China Graduate School of China Academy of Chinese Medical Sciences Beijing(100700)China Department of Andrology Beijing Chinese Medicine Hospital Afﬁliated to Capital Medical UniversityBeijing(100010)China Graduate School Beijing University of Chinese MedicineBeijing(100029)China

Objective: To observe the efficacy and safety of Guihuang Formula(GHF) in treating patients with type Ⅲ prostatitis and Chinese medicine syndrome of dampness-heat and blood stasis. Methods: Sixty-six type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome were randomly divided into the treatment group(GHF) and the control group(tamsulosin) using a random number table, with 33 cases each group. The treatment group received GHF twice a day, and the control group received tamsulosin 0.2 mg once daily before bedtime. Patients in both groups received treatment for 6 weeks and was followed up for 2 weeks. The outcomes included the National Institute of Health Chronic Prostatitis Symptom Index(NIH-CPSI) score, Chinese Medicine Symptoms Score(CMSS), expressed prostatic secretions(EPS) and adverse events(AEs). Results: After treatment, the NIH-CPSI total score and domain scores of pain discomfort, urination and quality of life decreased significantly from the baseline in both groups(P0.05). In addition, no serious AEs were observed. Conclusion: GHF is effective in treating type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome without serious AEs.(Registration No. ChiCTR1900026966)

关键词： typeⅢprostatitis dampness-heat and blood stasis syndrome Guihuang Formula Chinese medicine randomized controlled trial

Prognostic factors and its predictive value in patients with metastatic spinal cancer

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World Journal of Clinical Cases 2021年第20期9卷 5470-5478页

作者： qing-Peng gao Da-Zhi Yang Zheng-Bin Yuan Yu-Xia Guo Department of Spine Surgery The Second Clinical Medical College of Jinan UniversityShenzhen 518020Guangdong ProvinceChina Department of Spine Surgery Shenzhen People’s Hospital2nd Clinical Medical College of Jinan UniversityShenzhen 518020Guangdong ProvinceChina Department of Gynecology Shenzhen People’s Hospital2nd Clinical Medical College of Jinan UniversityShenzhen 518020Guangdong ProvinceChina

BACKGROUND The spine is the most common location of metastatic *** a metastatic spinal tumor depends on many factors,including patients’overall health and life *** present study was conducted to investigate prognostic factors and clinical outcomes in patients with vertebral *** To investigate prognostic factors and their predictive value in patients with metastatic spinal *** A retrospective analysis of 109 patients with metastatic spinal cancer was conducted between January 2015 and September *** prognoses and survival were analyzed,and the effects of factors such as clinical features,treatment methods,primary lesions and affected spinal segments on the prognosis of patients with metastatic spinal cancer were *** prognostic value of Frankel spinal cord injury functional classification scale,metastatic spinal cord compression(MSCC),spinal instability neoplastic score(SINS)and the revised Tokuhashi score for prediction of prognosis was explored in patients with metastatic spinal *** Age,comorbidity of metastasis from elsewhere,treatment methods,the number of spinal tumors,patient’s attitude toward tumors and Karnofsky performance scale score have an effect on the prognosis of patients(all P<0.05).With respect to classification of spinal cord injury,before operation,the proportion of grade B and grade C was higher in the group of patients who died than in the group of patients who survived,and that of grade D and grade E was lower in the group of patients who died than in the group of patients who survived(all P<0.05).At 1 mo after operation,the proportion of grade A,B and C was higher in the group of patients who died than in the group of patients who survived,and that of grade E was lower in patients in the group of patients who died than in the group of patients who survived(all P<0.05).MSCC occurred in four(14.3%)patients in the survival group and 17(21.0%)patients in the death group(P<0.05).

关键词： Metastatic spinal tumors Frankel spinal cord injury functional classification scale Metastatic spinal cord compression Spinal instability neoplastic score Revised Tokuhashi score

Tensile Elastic Modulus, Strength and Fracture of δ-Al_2O_(3f)/Al Alloy Composites

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Journal of Materials Science & Technology 2000年第5期16卷 475-480页

作者： GuoZheng KANG+, qing gao and Jixi ZHANG (Department of Applied Mechanics and Engineering, Southwest Jiaotong University, Chengdu 610031, China) Department of Applied Mechanics and Engineering Southwest Jiaotong University Chengdu 610031 China

Based on the experimental and theoretical analysis, the tensile elastic modulus, strength and fracture characteristics of squeeze casting δ-Al2O3/Al alloy composites were studied. The fracture characteristics of composites were observed by SEM. The elastic modulus was predicted by the finite element method based on the energy equivalence principle, and the strength was predicted by the statistical integration average method using the maximum energy criterion of composite strength. In the prediction, the distribution density functions of the fiber's. orientation and length were considered. These functions were gained by experimental measurement. It is shown that the predicted results are in agreement with the experimental values well and the microstructure feature of composites controls the fracture characteristics.

关键词： Tensile Elastic Modulus Al2O

Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring

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Nano-Micro Letters 2024年第2期16卷 347-364页

作者： Yuewei Chen Yanyan Zhou Zihe Hu Weiying Lu Zhuang Li Ning gao Nian Liu Yuanrong Li Jing He qing gao Zhijian Xie Jiachun Li Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China School of Mechanical Engineering Guizhou UniversityGuiyang 550025People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhou 310006People’s Republic of China

Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose ***,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant challenges for *** is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found naturally in most *** study used 3D printing ultrafine fiber networks with metamaterial design to embed into ultra-low elastic modulus hydrogel to create a novel gelatin-based conductive film(GCF)with mechanical *** regulation of GCF nearly covers soft tissue mechanics,an elastic modulus from 20 to 420 kPa,and a Poisson’s ratio from-0.25 to *** negative Poisson’s ratio promotes conformality with soft tissues to improve the efficiency of biological *** GCF can monitor heartbeat signals and respiratory rate by determining cardiac deformation due to its high ***,the gelatin characteristics of the biodegradable GCF enable the sensor to monitor and support tissue *** GCF metamaterial design offers a unique idea for bioelectronics to develop implantable sensors that integrate monitoring and tissue repair and a customized method for endowing implanted sensors to be highly conformal with soft tissues.

关键词： Implantable hydrogel-based bioelectronics Conformality 3D printing Metamaterial design