检索结果-南通市图书馆

Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2024年第2期7卷 179-188页

作者： Weidong qian Xuefei Wang Ting Wang Jie Huang qian zhang Yongdong Li Si Chen School of Food and Biological Engineering Shaanxi University of Science and TechnologyXi'anP.R.China Department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenP.R.China Ningbo Municipal Center for Disease Control and Prevention NingboP.R.China Medical College of Shenzhen University ShenzhenP.R.China

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat ***,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence *** RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated *** method also demonstrates excellent specificity without cross-reactivity to non-target ***,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,***,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health *** established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 *** RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

关键词： CRISPR-Cas12a detection human bocavirus 1 on-site diagnosis recombinase polymerase amplification

Clinical study on microscopic syndrome differentiation and traditional Chinese medicine treatment for liver stomach disharmony in chronic gastritis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2024年第5期16卷 1377-1384页

作者： Chun-Yan Bai Wei Tian qian zhang Department of Rehabilitation Medicine Beijing Aerospace General HospitalBeijing 100076China Department of Rehabilitation Medicine People’s Hospital of HengshuiHengshui 053000Hebei ProvinceChina Department of Internal Medicine Hebei Academy of Chinese Medicine SciencesShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Chronic gastritis(CG)is a common gastrointestinal disorder characterized by inflammation of the stomach ***-stomach disharmony(LSD)syndrome is believed to contribute to CG *** To evaluate the efficacy and safety of microcosmic syndrome differentiation and Chinese herbal medicine(CHM)treatment in patients with CG and LSD *** Sixty-four patients with CG and LSD syndrome were randomly divided into two groups:The treatment group received CHM based on microcosmic syndrome differentiation and the control group received conventional Western *** treatment course lasted 12 *** primary outcome was improvement in dyspeptic symptoms,measured using the Nepean Dyspepsia *** secondary outcomes included the improvement rate of endoscopic findings,histopathological findings,and microcosmic syndrome scores and the incidence of adverse *** After 12 wk of treatment,the treatment group showed significantly greater improvement in dyspeptic symptoms than the control group(93.75%vs 65.63%,P0.05).No serious adverse events were observed in either *** Microcosmic syndrome differentiation and CHM treatment can effectively improve dyspeptic symptoms and endoscopic findings in patients with CG and LSD syndrome and have a good safety *** studies with larger sample sizes and longer follow-up periods are required to confirm the long-term efficacy and mechanism of action of this treatment.

关键词： Chronic gastritis Liver-stomach disharmony Microcosmic syndrome differentiation Chinese herbal medicine Randomized controlled trial Microcosmic syndrome scores

Kinetics insights into size effects of carbon nanotubes'growth and their supported platinum catalysts for 4,6-dinitroresorcinol hydrogenation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2024年第8期72卷 133-140页

作者： Yan zhang Xiangxue zhang Keng Sang Wenyao Chen Gang qian Jing zhang Xuezhi Duan Xinggui Zhou Weikang Yuan State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai 200237China

Size effects are a well-documented phenomenon in heterogeneous catalysis,typically attributed to alterations in geometric and electronic *** this study,we investigate the influence of catalyst size in the preparation of carbon nanotube(CNT)and the hydrogenation of 4,6-dinitroresorcinol(DNR)using Fe_(2)O_(3)and Pt catalysts,*** Fe_(2)O_(3)/Al_(2)O_(3)catalysts were synthesized for CNT growth through catalytic chemical vapor *** findings reveal a significant influence of Fe_(2)O_(3)nanoparticle size on the structure and yield of ***,CNT produced with Fe_(2)O_(3)/Al_(2)O_(3)containing 28%(mass)Fe loading exhibits abundant surface defects,an increased area for metal-particle immobilization,and a high carbon *** makes it a promising candidate for DNR *** this catalyst support,we further investigate the size effects of Pt nanoparticles on DNR *** Pt catalysts demonstrate a preference for 4,6-diaminoresorcinol generation at(100)sites,whereas smaller Pt catalysts are more susceptible to electronic *** kinetics insights obtained from this study have the potential to pave the way for the development of more efficient catalysts for both CNT synthesis and DNR hydrogenation.

关键词： Kinetics Size effects Catalytic hydrogenation Active site

Quantum weak force sensing with squeezed magnomechanics

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2024年第10期67卷 23-32页

作者： qian zhang Jie Wang Tian-Xiang Lu Ran Huang Franco Nori Hui Jing Key Laboratory of Low-Dimensional Quantum Structures and Quantum Control of Ministry of Education Department of Physics and Synergetic Innovation Center for Quantum Effects and ApplicationsHunan Normal UniversityChangsha 410081China Theoretical Quantum Physics Laboratory Cluster for Pioneering ResearchRIKENWako-shi 351-0198Japan Center for Quantum Computing RIKENWako-shi 351-0198Japan Department of Physics University of MichiganAnn Arbor 48109-1040USA College of Physics and Electronic Information Gannan Normal UniversityGanzhou 341000China Academy for Quantum Science and Technology Zhengzhou University of Light IndustryZhengzhou 450002China

Cavity magnomechanics,exhibiting remarkable experimental tunability,rich magnonic nonlinearities,and compatibility with various quantum systems,has witnessed considerable advances in recent ***,the potential benefits of using cavity magnomechanical(CMM)systems in further improving the performance of quantum-enhanced sensing for weak forces remain largely *** we show that,by squeezing the magnons,the performance of a quantum CMM sensor can be significantly enhanced beyond the standard quantum limit(SQL).We find that,for comparable parameters,two orders of magnitude enhancement in the force sensitivity can be achieved in comparison with the case without magnon ***,we obtain the optimal parameter regimes of homodyne angle for minimizing the added quantum *** findings provide a promising approach for highly tunable and compatible quantum force sensing using hybrid CMM devices,with potential applications ranging from quantum precision measurements to quantum information processing.

关键词： quantum force sensing cavity magnomechanics magnon Kerr effect

Sex-biased single-cell genetic landscape in mice with autism spectrum disorder

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第3期51卷 338-351页

作者： qian zhang Yongjie Wang Jie Tao Ruixue Xia Yijie zhang Zhirui Liu Jiwei Cheng Department of Neurology and Central Laboratory Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai 200062China School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang 311121China Department of Respiratory and Critical Care Medicine Henan University Huaihe HospitalKaifengHenan 475099China

Autistic spectrum disorder(ASD)is a male-biased,heterogeneous neurodevelopmental disorder that affects approximately 1%e2%of the *** exposure to valproic acid(VPA)is a recognized risk factor for ASD,but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is ***,we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic *** transcriptomes of more than 45,000 cells are assigned to 12 major cell types,including neurons,glial cells,vascular cells,and immune *** type-specific genes with altered expression after prenatal VPA exposure are analyzed,and the largest number of differentially expressed genes(DEGs)are found in neurons,choroid plexus epithelial cells,and *** microglia,several pathways related to inflammation are found in both males and females,including the tumor necrosis factor(TNF),nuclear factor kappa B(NF-kB),toll-like receptor(TLR),and mitogen-activated protein kinase(MAPK)signaling pathways,which are important for the induction of autistic-like ***,we note that several X-linked genes,including Bex1,Bex3,and Gria3,were among the male-specific DEGs of *** pioneering study describes the landscape of the transcriptome in the hippocampus of autistic *** elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.

关键词： Autistic spectrum disorder(ASD) Sex Single cell Neurons Microglia

A macro-mesoscopic constitutive model for porous and cracked rock under true triaxial conditions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rock Mechanics and Geotechnical Engineering 2024年第8期16卷 3080-3098页

作者： Li qian Zuguo Mo Jianhai zhang Xianglin Xing Ru zhang Tianzhi Yao Yunpeng Gao State Key Laboratory of Hydraulics and Mountain River Engineering College of Water Resources and HydropowerSichuan UniversityChengdu610065China Power China Chengdu Engineering Corporation Limited Chengdu610072China

The complex mechanical and damage mechanisms of rocks are intricately tied to their diverse mineral compositions and the formation of pores and cracks under external *** rock tests reveal a complex interplay between the closure of porous defects and the propagation of induced cracks,presenting challenges in accurately representing their mechanical properties,especially under true triaxial stress *** paper proposes a conceptualization of rock at the mesoscopic level as a two-phase composite,consisting of a bonded medium matrix and frictional medium *** bonded medium is characterized as a mesoscopic elastic material,encompassing various minerals surrounding porous *** mechanical properties are determined using the mixed multi-inclusion *** of the bonded medium into the frictional medium occurs through crack extension,with its elastoplastic properties defined by the DruckerePrager yield criterion,accounting for hardening,softening,and *** and Eshelby’s equivalent inclusion methods are applied to the bonded and frictional media,*** macroscopic mechanical properties of the rock are derived from these mesoscopic ***,a True Triaxial Macro-Mesoscopic(TTMM)constitutive model is *** model effectively captures the competitive effect and accurately describes the stress-deformation characteristics of *** the TTMM model,the strains resulting from porous defect closure and induced crack extension are differentiated,enabling quantitative determination of the associated damage evolution.

关键词： Micromechanics Macroemesoscopic Homogenization Constitutive model Competitive effect

Stability analysis of loose accumulation slopes under rainfall:case study of a high‑speed railway in Southwest China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Railway Engineering Science 2024年第1期32卷 95-106页

作者： Xin Wang qian Su Zongyu zhang Feihu Huang Chenfang He School of Civil Engineering Southwest Jiaotong UniversityChengdu 610031China Key Laboratory of High‑Speed Railway Engineering Ministry of Education School of Civil EngineeringSouthwest Jiaotong UniversityChengdu 610031China CCCC Second Highway Consultants Co. Ltd.Wuhan 430056China

The high and steep slopes along a high-speed railway in the mountainous area of Southwest China are mostly composed of loose accumulations of debris with large internal pores and poor stability,which can easily induce adverse geological disasters under rainfall *** ensure the smooth construction of the high-speed railway and the subsequent safe operation,it is necessary to master the stability evolution process of the loose accumulation slope under *** article simulates rainfall using the finite element analysis software’s hydromechanical coupling *** slope stability under various rainfall situations is calculated and analysed based on the strength reduction *** validate the simulation results,a field monitoring system is established to study the deformation characteristics of the slope under *** results show that rainfall duration is the key factor affecting slope *** a constant amount of rainfall,the stability of the slope decreases with increasing duration of ***,when the amount and duration of rainfall are constant,continuous rainfall has a greater impact on slope stability than intermittent *** setting of the field retaining structures has a significant role in improving slope *** field monitoring data show that the slope is in the initial deformation stage and has good stability,which verifies the rationality of the numerical simulation *** research results can provide some references for understanding the influence of rainfall on the stability of loose accumulation slopes along high-speed railways and establishing a monitoring system.

关键词： High-speed railway Loose accumulation slope Slope stability analysis Rainfall effect Strength reduction

Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第1期17卷 290-296页

作者： Hangbin Lei qian zhang Xiaoyan Xiang Liang Jiang Shiyan Wang Lingxuan Duan Shizhen Wang Department of Chemical and Biochemical Engineering College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Xiamen Key Laboratory of Synthetic Biotechnology Xiamen UniversityXiamen 361005China

Immobilization of D-amino acid dehydrogenase(DAADH)by the assembly of peptide linker was studied for the biosynthesis of *** material of zeolitic imidazolate framework-8(ZIF-8)combined with reduced graphene oxide(RGO)was applied for the immobilization of DAADH from Ureibacillus *** recovery rate of DAADH/ZIF-8/RGO was 165.6%.DAADH/ZIF-8/RGO remained 53.4%of its initial activity at 50°C for 10 h while the free enzyme was ***/ZIF-8/RGO maintained 70.5%activity in hyperalkaline solution with pH *** parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as V_(max)/K_(m)of DAADH/ZIF-8/RGO was 1.27-fold than free *** seven recycles,the activity of DAADH/ZIF-8/RGO remained 64.3%.Furthermore,one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity *** peptide linker and metal-organic framework(MOF)immobilization,thermostability and activity of the immobilized DAADH were significantly improved.

关键词： D-Amino acid dehydrogenase immobilization metal-organic frameworks zeolitic imidazolate framework-8(ZIF-8) reduced graphene oxide

Advancements in synthetic and electrocatalytic exploitation of guest-host intercalated 2D materials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2024年第3期34卷 454-469页

作者： qian Yang Kun Wang Meiling zhang Feihong Du Jiayang Li Chen Zheng Jinyang Li School of Chemistry Southwest Jiaotong University Yibin Institute of Southwest Jiaotong University

The emergence and development of layered materials have shown great promise for many applications, especially in recent years, the effective modulation of the physicochemical properties of layered materials by intercalation chemistry has led to great potential in material design. In this paper, we review the reaction mechanisms of different guest species(metal atoms/ions, organic molecules/ions, inorganic molecules and inorganic ions)intercalated into the host layered materials as well as the recent progress. It is also reviewed that layered intercalated materials with controllable structure and tunable physicochemical properties can be prepared through the occurrence of interactions between different subjects and guests. The specific applications of intercalation chemistry in the field of electrocatalysis, such as the hydrogen evolution reaction, oxygen evolution reaction, oxygen reduction reaction and other electrocatalytic reactions, are then discussed, with emphasis on the mechanism of the improved catalytic activity and stability of the layered materials after guest intercalation.

关键词： Layered materials Intercalation chemistry Guest species Electrocatalysis