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1,3-Bis(2-chloroethyl)-1-nitrosourea enhances the inhibitory effect of Resveratrol on 5-fluorouracil sensitive/resistant colon cancer cells

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World Journal of Gastroenterology 2013年第42期19卷 7374-7388页

作者： Dipon Das Ranjan Preet purusottam mohapatra Shakti Ranjan Satapathy Chanakya Nath Kundu Cancer Biology Division KIIT School of BiotechnologyKIIT UniversityBhubaneswarOdisha 751024India

AIM:To study the mechanism of 5-fluorouracil(5-FU)resistance in colon cancer cells and to develop strategies for overcoming such resistance by combination ***:We established and characterized a 5-FU resistance(5-FU-R)cell line derived from continuous exposure(25μmol/L)to 5-FU for 20 wk in 5-FU sensitive HCT-116 *** proliferation and expression of different representative apoptosis and anti-apoptosis markers in 5-FU sensitive and 5-FU resistance cells were measured by the MTT assay and by Western blotting,respectively,after treatment with Resveratrol(Res)and/or 1,3-Bis(2-chloroethyl)-1-nitrosourea(BCNU).Apoptosis and cell cycle arrest was measured by 4',6'-diamidino-2-phenylindole hydrochloride staining and fluorescence-activated cell sorting analysis,*** extent of DNA damage was measured by the Comet *** measured the visible changes in the DNA damage/repair cascade by Western ***:The widely used chemotherapeutic agents BCNU and Res decreased the growth of 5-FU sensitive HCT-116 cells in a dose dependent *** application of BCNU and Res caused more apoptosis in5-FU sensitive cells in comparison to individual *** addition,the combined application of BCNU and Res caused a significant decrease of major DNA base excision repair components in 5-FU sensitive *** established a 5-FU resistance cell line(5-FU-R)from 5-FU-sensitive HCT-116(mismatch repair deficient)cells that was not resistant to other chemotherapeutic agents(e.g.,BCNU,Res)except *** 5-FU resistance of 5-FU-R cells was assessed by exposure to increasing concentrations of 5-FU followed by the MTT *** was no significant cell death noted in5-FU-R cells in comparison to 5-FU sensitive cells after5-FU *** resistant cell line overexpressed anti-apoptotic[e.g.,AKT,nuclear factorκB,FLICE-like inhibitory protein),DNA repair(e.g.,DNA polymerase beta(POL-β),DNA polymerase eta(POLH),protein Flap endonuclease 1(FEN1),DNA dam

关键词： 5-fluorouracil 1,3-Bis(2-chloroethyl)-1-nitrosourea Resveratrol Colon cancer Combination therapy

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Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization

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Asian pacific Journal of Reproduction 2018年第2期7卷 79-86页

作者： Madhusmita Panda Asmita Sandeep Kumar purusottam Mishra Mahesh Chandra Sahu Sachinandan De Tirtha Kumar Datta Rakesh Kumar Nodal Officer Chief District Veterinary OfficeKadambedaBhadrak756100OdishaIndia Institute of Microbial Technology Council of Scientific and Industrial ResearchChandigarhIndia BSL-4 National Institute of VirologySus RoadPashanPune-411021India Goat Breeding Farm AT/PO-SalapadaDistrict-KeonjharPin code-758020State-OdishaIndia Directorate of Medical Research IMS and SUM HospitalSiksha'O'Anusandhan UniversityK8Kalinga NagarBhubaneswar-751003OdishaIndia Animal Biotechnology Centre National Dairy Research InstituteKarnal132001HaryanaIndia

Objective:To understand the level of progesterone (P4) in different quality of buffalo cumulus oocyte complexes (COCs) and further to evaluate the effect of exogenous P4 supplementation on maturation and subsequent developmental ability of poor quality brilliant cresyl blue (BCB-)***: Progesterone secreted by different quality of buffalo oocytes was estimated by enzyme linked immunosorbent assay and the concentration differences were translated into P4 doses to be incorporated in the maturation medium of BCB-ve COCs followed by expression analysis of genes involved in the cumulus expansion, extracellular matrix disintegration and progesterone receptor signalling. In addition, the study also evaluated the effect of exogenous P4 on sperm-cumulus interaction. Results: More than 10-fold up-regulated expression of progesterone receptor in P4 supplemented oocytes signified that P4 might be acting predominantly through this receptor. Also, exogenous P4 supplementation had significant effect on transcatheter arterial chemoembolization protease regulated by P4-progesterone receptor pathway which in turn had an important role in extracellular matrix disintegration. On the contrary, cumulus expansion genesHAS2,TNFAIP6,AREG were not altered upon P4 supplementation. Also, it was observed that P4 addition did facilitate passage of significantly more number of spermatozoa through P4 treated cumulus cells. Further, incorporation of different doses of P4 did not improve significantly the cleavage and blastocyst rates of BCB-ve ***: Different qualities of buffalo COCs secrete substantially diverse levels of P4, and its supplementation has a role in oocyte maturation via modulation of cumulus characteristics but perhaps not fertilization.

关键词： BCB screening Progesterone Sperm cumulus interaction Fertilizing ability

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