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Transcriptome analysis in the beet webworm, Spoladea recurvalis （Lepidoptera： Crambidae）

Insect Science 2018年第1期25卷 33-44页

作者： Jian-cheng chang Srinivasan Ramasamy A VRDC - The World Vegetable Center Shanhua Tainan Taiwan China

The beet webworm, Spoladea recurvalis Fabricius, is a destructive pest on vegetable crops in tropics and subtropics; its main host plant is amaranth. It has become imperative to develop non-chemical methods to control S. recurvalis on amaranth. However, the lack of molecular information about this species has hindered the development of novel pest management strategies. In this study, high-throughput RNA sequencing covering de novo sequence assemblies, functional annotation of transcripts, gene function classification and enrichment was performed on S. recurvalis. Illumina sequencing generated a total of 120 435 transcript contigs ranging from 201 to 22 729 bases with a mean length of 688 bases. The assembled transcripts were subjected to Basic Local Alignment Search Tool- X （BLASTX） to obtain the annotations against non-redundant, Swiss-Prot, Clusters of Orthologous Groups （COG） and Kyoto Encyclopedia of Genes and Genomes （KEGG） protein databases. A subset of 58 225 transcript sequences returned hits from known proteins in the National Center for Biotechnology Information database, and the majority of the transcript sequences had the highest number of hits for Danausplexippus （50.43%）. A total of 1217 Gene Ontology-level 3 annotations were assigned to 51 805 transcripts, while 39 650 transcripts were predicted as functional protein-coding genes in the COG database and 20 037 transcripts were enriched to KEGG pathways. We identified 40 putative genes related to pheromone production and reception in S. recurvalis, with the expression of one gene between 0.29 and 1141.79 fragments per kilo base per million （FPKM） reads. The transcriptome sequence of S. recurvalis is a first step toward offering a comprehensive genomic resource which would enable better understanding of molecular mechanisms to enable development of effective pest management practices for this species.

关键词： pheromone protein-coding genes putative genes Spoladea recurvalis transcriptome

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Distinguish models mimicking the SM Higgs

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Frontiers of physics 2013年第3期8卷 285-293页

作者： Jung chang Kingman Cheung po-Yan Tseng Tzu-Chiang Yuan Department of Physics Tsing Hua University Hsinchu 300 Taiwan China Division of Quantum Phases and Devices School of Physics Konkuk University Seoul 143-701 Republic of Korea Institute of Physics Academia Sinica Nangang Taipei 11529 Taiwan China

The new particle around 125 GeV observed at the LHC is almost consistent with the standard model Higgs boson, except that the diphoton decay mode may be excessive. We summarize a number of possibilities. We propose to use the vector-boson fusion to test the underlying model for electroweak symmetry breaking. Using the well known dijet-tagging technique to single out the vector-boson fusion mechanism, we investigate potential of vector-boson fusion to discriminate a number of models suggested to give an enhanced inclusive diphoton production rate.

关键词： Higgs boson vector-boson fusion diphoton electroweak svmmetrv breaking

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Advancements in adoptive CAR immune cell immunotherapy synergistically combined with multimodal approaches for tumor treatment

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Bioactive Materials 2024年第12期42卷 379-403页

作者： Yun chang Mingyang chang Xiaoping Bao cheng Dong Department of Biomedical Engineering The Hong Kong Polytechnic University999077Hong KongChina The Hong Kong polytechnic University Shenzhen Research Institute Shenzhen518000China Davidson School of Chemical Engineering Purdue UniversityWest LafayetteIN47907USA Purdue University Institute for Cancer Research West LafayetteIN47907USA

Adoptive immunotherapy,notably involving chimeric antigen receptor(CAR)-T cells,has obtained Food and Drug Administration(FDA)approval as a treatment for various hematological malignancies,demonstrating promising preclinical efficacy against ***,the intricate and resource-intensive autologous cell processing,encompassing collection,expansion,engineering,isolation,and administration,hamper the efficacy of this therapeutic ***,conventional CAR T therapy is presently confined to addressing solid tumors due to impediments posed by physical barriers,the potential for cytokine release syndrome,and cellular exhaustion induced by the immunosuppressive and heterogeneous tumor ***,a strategic integration of adoptive immunotherapy with synergistic multimodal treatments,such as chemotherapy,radiotherapy,and vaccine therapy etc.,emerges as a pivotal approach to surmount these inherent *** collaborative strategy holds the key to addressing the limitations delineated above,thereby facilitating the realization of more precise personalized therapies characterized by heightened therapeutic *** synergistic strategy not only serves to mitigate the constraints associated with adoptive immunotherapy but also fosters enhanced clinical applicability,thereby advancing the frontiers of therapeutic precision and effectiveness.

关键词： Adoptive cellular immunotherapy Immune cell engineering Multiple-model synergistic therapy Tumor microenvironment

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High-Voltage Aqueous Zinc Batteries Achieved by Tri-functional Metallic Bipolar Electrodes

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Energy & Environmental Materials 2023年第1期6卷 360-367页

作者： chang Liu Xiaowei Chi cheng Yang Yu Liu Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China

Aqueous rechargeable zinc batteries are very attractive for energy storage applications due to their low cost and high ***,low operating voltages limit their further *** the first time,this work proposes a unique approach to increase the voltages of aqueous zinc batteries by using tri-functional metallic bipolar electrode with good electrochemical activity and ultrahigh electronic conductivity,which not only participates in redox reactions,but also functions as an electrical highway for charge ***,bipolar electrode can replace expensive ion selective membrane to separate electrolytes with different pH;thus,redox couples with higher potential in acid condition and Zn=Zn(OH)^(2-)_(4) couple with lower potential in alkaline condition can be employed together,leading to high voltages of aqueous zinc ***,two types of metallic bipolar electrodes of Cu and Ag are utilized based on three kinds of aqueous zinc batteries:Zn–MnO_(2),Zn–I_(2),and Zn–Br_(2).The voltage of aqueous Zn–MnO_(2) battery is raised to 1.84 V by employing one Cu bipolar electrode,which shows no capacity attenuation after 3500 ***,the other Ag bipolar electrode can be adopted to successfully construct Zn–I_(2) and Zn–Br_(2) batteries exhibiting much higher voltages of 2.44 and 2.67 V,which also show no obvious capacity degradation for 1000 and 800 cycles,representing decent cycle *** bipolar electrode can be applied in a large family of aqueous batteries,this work offers an elaborate high-voltage concept based on tri-functional metallic bipolar electrode as a model system to open a door to explore high-voltage aqueous batteries.

关键词： aqueous zinc battery high voltage metallic bipolar electrode

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Transient ischemic attack after mRNA-based COVID-19 vaccination during pregnancy:A case report

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World Journal of Clinical Cases 2022年第27期10卷 9929-9935页

作者： Chi-Han chang Sheng-po Kao Dah-Ching Ding Department of Obstetrics and Gynecology Hualien Tzu Chi HospitalBuddhist Tzu Chi Medical FoundationTzu Chi UniversityHualien 970Taiwan

BACKGROUND Thrombocytopenia with thrombosis syndrome has been reported after vaccination against severe acute respiratory syndrome coronavirus 2 with two mRNA *** syndrome is characterized by thrombosis,especially cerebral venous sinus thrombosis,and may lead to *** women with stroke show higher rates of pregnancy loss and experience serious pregnancy *** present the case of a 24-year-old pregnant woman with a transient ischemic attack(TIA) that developed after vaccination with the Moderna mRNA-1273 vaccine(at 37 2/7 wk).CASE SUMMARY TIA occurred 13 d following the coronavirus disease *** 39 1/7 wk of pregnancy,the patient presented with sudden onset of right eye blurred vision with headache,dizziness with nausea,right-hand weakness,anomia,and *** symptoms lasted 3 h;TIA was *** test results revealed elevated D-dimer,cholesterol,and triglyceride *** magnetic resonance imaging showed no acute hemorrhagic or ischemic *** pregnancy 37 6/7 wk,she was admitted for cesarean delivery to reduce subsequent risk of stroke during *** mass index on admission was 19.8 kg/m~*** resonance angiography and transesophageal echocardiography showed no *** next day,a mature female baby weighing 2895 g and measuring 50 cm was *** scores were 8 and 9 in the first and fifth minutes.D-dimer levels decreased on postoperative day *** discharge,the autoimmune panel was within normal limits,including antinuclear and antiphospholipid *** TIA might be developed after the mRNA vaccines in pregnant women.

关键词： Pregnancy mRNA vaccine COVID-19 Stroke Transient ischemic attack Case report

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Long Wave Reflection and Transmission over A Sloping Step

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海洋工程：英文版 2004年第3期18卷 371-380页

作者： Hsien-Kuo chang Jin-cheng LIOU Department of Civil Engineering National Chiao Tung University 1001 Ta Hsuech Road Hsinchu 300 ChinaDepartment of Civil Engineering National Chiao Tung University 1001 Ta Hsuech Road Hsinchu 300 China

This investigation examines long wave reflection and transmission induced by a sloping step. Bellman and Kalaba's (1959) invariant imbedding is introduced to find wave reflection. An alternative method matching both the surface elevation and its surface slope of each region at the junction is applied to the determination of wave reflection and transmission. The proposed methods are compared with the accurate numerical results of porter and porter (2000) and those of Mei (1983) for a vertical step. The wave reflection obtained for a mildly sloping step differs significantly from the result of Mei. The wave reflection is found to fluctuate owing to wave trapping for the mild sloping step. The height and the face slope of the step are important for determining wave reflection and transmission coefficients.

关键词： long wave reflection coefficient wave transmission wave trapping

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Geochemical analysis of mixed oil in the Ordovician reservoir of the Halahatang Depression,Tarim Basin,China

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Chinese Journal Of Geochemistry 2013年第4期32卷 347-356页

作者： cheng Bin WANG Tieguan chang Xiangchun State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum Shandong University of Science and Technology

In this study,12 crude oil samples were collected and analyzed from the Ordovician reservoir in the Halahatang Depression,Tarim Basin,*** the density of oil samples varies considerably,based on saturated hydrocarbon gas chromatographic(GC),saturated and aromatic hydrocarbon gas chromatographic-mass spectrometric(GC/MS) and stable carbon isotopic composition analyses,all the samples are interpreted to represent a single oil population with similar characteristics in a source bed or a source kitchen,organic facies and even in oil charge *** co-existence of a full suite of n-alkanes and acyclic isoprenoids with UCM and 25-norhopanes in the crude oil samples indicates mixing of biodegraded oil with fresher non-biodegraded oil in the Ordovician ***,according to the conversion diagram of double filling ratios for subsurface mixed crude oils,biodegraded/non-biodegraded oil ratios were determined as in the range from 58/42 to 4/*** on oil density and oil mix ratio,the oils can be divided into two groups:Group 1,with specific density>0.88(g/cm3) and oil mix ratio>1,occurring in the north of the Upper Ordovician Lianglitage and Sangtamu Formation pinchout lines,and Group 2,with specific density<0.88(g/cm3) and oil mix ratio<1,occurring in the south of the pinchout ***,Group 2 oils with low densities and being dominated by non-biodegraded oils are better than Group 1 oils with respect to *** is suggested that more attention should be paid to the area in the south of the Upper Ordovician Lianglitage and Sangtamu Formation pinchout lines for further exploration.

关键词：塔里木盆地地球化学分析奥陶系混合油储层中国生物降解油密度变化

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Anatomizing the Ocean's Role in Maintaining the Pacific Decadal Variability

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Advances in Atmospheric Sciences 2014年第3期31卷 611-623页

作者： Jia-Yuh YU cheng-Wei chang Department of Atmospheric Sciences Chinese Culture University Graduate Institute of Earth Science Chinese Culture University

ABSTRACT The role of ocean dynamics in maintaining the Pacific Decadal Variability （PDV） was investigated based on simulation results from the Parallel Ocean Program （poP） ocean general circulation model developed at the Los Alamos National Laboratory （LANL）. A long-term control simulation of the LANL-poP model forced by a reconstructed coupled wind stress field over the period 1949-2001 showed that the ocean model not only simulates a reasonable climatology, but also produces a climate variability pattem very similar to observed PDV. In the Equatorial Pacific （EP） region, the decadal warming is confined in the thin surface layer. Beneath the surface, a strong compensating cooling, accompanied by a basin-wide-scale overturning circulation in opposition to the mean flow, occurs in the thermocline layer. In the North Pacific （NP） region, the decadal variability nonetheless exhibits a relatively monotonous pattern, characterized by the dominance of anomalous cooling and eastward flows. A term balance analysis of the perturbation heat budget equation was conducted to highlight the ocean＇s role in main- taining the PDV-like variability over the EP and NP regions. The analyses showed that strong oceanic adjustment must occur in the equatorial thermocline in association with the anomalous overturning circulation in order to maintain the PDV-like variability, including a flattening of the equatorial thermocline slpoe and an enhancement of the upper ocean＇s stratification （stability）, as the climate shifts from a colder regime toward a warmer one. On the other hand, the oceanic response in the extratropical region seems to be confined to the surface layer, without much participation from the subsurface oceanic dynamics.

关键词： Pacific decadal variability term balance analysis perturbation heat budget equation

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Asymptotic behavior for sums of non-identically distributed random variables

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Applied Mathematics(A Journal of Chinese Universities) 2019年第1期34卷 45-54页

作者： YU chang-jun cheng Dong-ya School of Sciences Nantong University School of Mathematical Sciences Soochow University The Statistics and Operations Research Department the University of North Carolina at Chapel Hill

For any given positive integer m, let X_i, 1 ≤ i ≤ m be m independent random variables with distributions F_i, 1 ≤ i ≤ m. When all the summands are nonnegative and at least one of them is heavy-tailed, we prove that the lower limit of the ratio ■equals 1 as x →∞. When the summands are real-valued, we also obtain some asymptotic results for the tail probability of the sums. Besides, a local version as well as a density version of the above results is also presented.

关键词： lower limits upper limits heavy-tailed distributions local distributions densities

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HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

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World Journal of Gastroenterology 2010年第40期16卷 5092-5103页

作者： Mong-Liang Chen Kuan-Der Lee Huei-Chun Huang Yue-Lin Tsai Yi-Chieh Wu Tzer-Min Kuo cheng-po Hu Chungming chang Division of Molecular and Genomic Medicine National Health Research Institutes Department of Hematology and Oncology Chang Gung Memorial Hospital Institute of Microbiology and Immunology National Yang-Ming University Department of Life Science Tunghai University

AIM: To investigate the differentiation status and key factors to facilitate hepatic differentiation of human bone-marrow-derived mesenchymal stem cells (MSCs). METHODS: Human MSCs derived from bone marrow were induced into hepatocyte-like cells following a previously published protocol. The differentiation status of the hepatocyte-like cells was compared with various human hepatoma cell lines. Overexpression of hepatocyte nuclear factor (HNF)-4α was mediated by adenovirus infection of these hepatocyte-like cells. The expression of interesting genes was then examined by either re-verse transcription-polymerase chain reaction (RT-PCR) or real-time RT-PCR methods. RESULTS: Our results demonstrated that the differentiation status of hepatocyte-like cells induced from human MSCs was relatively similar to poorly differentiated human hepatoma cell lines. Interestingly, the HNF-4 isoform in induced MSCs and poorly differentiated human hepatoma cell lines was identified as HNF4γ instead of HNF-4α. Overexpression of HNF-4α in induced MSCs significantly enhanced the expression level of hepatic-specific genes, liver-enriched transcription factors, and cytochrome P450 (P450) genes. CONCLUSION: Overexpression of HNF-4α improves the hepatic differentiation of human MSCs from bone marrow and is a simple way of providing better cell sources for clinical applications.

关键词： Bone marrow Cytochrome P450 genes Differentiation of hepatocyte Hepatocyte nuclear factor 4 Human mesenchymal stem cells

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