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Journal of Iron and Steel Research International 2017年第3期24卷 251-257页

作者： Chao Zhou Xing-zhong Zhang Fang Wang ping-fei liu Yi-ming Fang National Engineering Research Center for Equipment and Technology of Cold Strip Rolling Yanshan University Qinhuangdao 066004 Hebei China Hebei Agricultural University Qinhuangdao 066003 Hebei China School of Science Yanshan University Qinhuangdao 066004 Hebei China

Due to the disadvantages of complexity,high maintenance and vast investment of the electro-hydraulic servo oscillator,a new mechanical device synchronously driven by double servomotors was proposed. The working principle of the non-sinusoidal oscillator was analyzed and the model of the oscillator was validated via simulation ***,taking advantage of resonance technology,the hinging force and moment were calculated. The results showed that the hinging force and driving moment reduced,which was useful in reducing the impact of the hinge and prolonging the service life of the bearing. Besides,the best initial spring pressure was 0. 9 times the mold gravity,which improved the oscillation system stability and reduced the load fluctuation and servomotor driving power.

关键词： Continuous casting Mold Non-sinusoidal oscillation Resonance technology Oscillation mechanism

Hepatitis B virus X protein promotes proliferation and upregulates TGF-β1 and CTGF in human hepatic stellate cell line,LX-2

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Hepatobiliary & Pancreatic Diseases International 2009年第1期8卷 59-64页

作者： Guang-Hui Guo,De-Ming Tan,ping-An Zhu and fei liu Department of Infectious Diseases,Xiangya Hospital,Central South University,Changsha 410008,China Department of Infectious Diseases Xiangya Hospital Central South University Changsha 410008 China. guanghuiguo2008@***

BACKGROUND:Chronic hepatitis B virus(HBV)infection is a major cause of liver fibrosis,but the mechanisms underlying HBV-related fibrogenesis are still unknown. Although the roles of HBV X protein(HBx)remain poorly understood,it is thought to play an important role in the regulation of cellular growth and hepatocarcinogenesis. The aim of this study was to determine the role of HBx in liver fibrogenesis by studying the effect of HBx on the proliferation and expression of fibrosis-related molecules in the human hepatic stellate cell line,LX-2. METHODS:We established an in vitro co-culture system with LX-2 cells and a stable QSG7701-HBx cell line which had been transfected with the HBx gene. 3 H-TdR incorporation and flow cytometry were used to determine the effects of HBx on the proliferation of LX-2 cells. α-smooth muscle actin(α-SMA),transforming growth factor-β1(TGF-β1),transforming growth factor-βreceptor Ⅱ(TGF-βRⅡ),and connective tissue growth factor (CTGF)in LX-2 cells were analyzed by Western *** addition,the expression levels of collagen typeⅠ(ColⅠ) from the co-cultured media were measured by ELISA. RESULTS: 3 H-TdR incorporation increased significantly in LX-2 cells co-cultured with QSG7701-HBx cells compared to those cultured with QSG7701-pcDNA3 and QSG7701 (non-tumorigenic human liver cell line).Cell cycle results revealed that HBx accelerated the progression of G1 to S in LX-2 *** expressions ofα-SMA,TGF-β1,TGF-βR Ⅱ,CTGF and ColⅠwere significantly increased in the co- cultures of LX-2 cells with stable QSG7701-HBx cells. CONCLUSION:These results suggest that HBx may facilitate liver fibrosis by promoting hepatic stellate cell proliferation and upregulating the expression of fibrosis- related molecules.

关键词： hepatitis B virus HBx liver cirrhosis hepatic stellate cell proliferation

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： fei Lv Longjie liu Jiazhe Wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

A Novel Nonsense Mutation in CRYGC Associated with Autosoreal Dominant Congenital Nuclear Cataract and Microcornea

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A Novel Nonsense Mutation in CRYGC Associated with Autosorea...

High-resolution imaging through dynamic scattering media: Iterative optimization based on polarimetric characteristics

中华医学会第十二届全国眼科学术大会

作者： Lu Zhang ping liu fei Leng Guangsen Shi Eye hospital,the First Affiliated Hospital,Harbin Medical University,23 Youzheng Road,Harbin 150001,China

Purpose:To report the identification of a novel nonsense mu- tation of CRYGC in aChinese family with autosomal dominant congenital nuclear cataract ***:We in- vestigated a four—generation Chinese family with nuclear ...

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Science China Technological Sciences 2025年第1期 82-84页

作者： Meng XIANG Kai DENG Tianyu WANG Jinpeng liu Xue DONG Teli XI fei liu Xiaopeng SHAO School of Optoelectronic Engineering Xidian University Xi'an Key Laboratory of Computational Imaging National Key Laboratory of Infrared Detection Technologies

High-resolution imaging through randomly dynamic scattered fields and highly scattered walls is an extensively sought-after capability with potential applications in various fields such as underwater imaging, biomedical imaging, and seeing through fog. Numerous methods have been proposed to unscramble object information from degraded scattered images, resulting in considerable improvements in image contrast in degraded scenarios [1].

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升降散改善MNNG诱导的胃黏膜损伤及其机制

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中国药理学通报 2025年第1期41卷 178-185页

作者：刘轶璜张思佳刘菲陈苹苹河北中医药大学药学院河北石家庄050091 河北省离子通道功能与创新中药国际联合研究中心河北石家庄050091 河北中医药大学中西医结合学院河北石家庄050091

目的确定升降散对胃黏膜损伤的改善作用并探讨其机制。方法通过文献计量学研究了解升降散的研究现状。采用液质联用技术获取升降散主要药效成分,通过网络药理学分析预测机制。对胃黏膜损伤大鼠灌胃给予阳性药及不同剂量升降散,通过HE染... 详细信息

目的确定升降散对胃黏膜损伤的改善作用并探讨其机制。方法通过文献计量学研究了解升降散的研究现状。采用液质联用技术获取升降散主要药效成分,通过网络药理学分析预测机制。对胃黏膜损伤大鼠灌胃给予阳性药及不同剂量升降散,通过HE染色,观察胃组织形态。ELISA实验检测血清中TNF-α、IL-1β的水平。IHC实验评价胃黏膜MUC5AC、MUC6的表达。结果文献计量学分析表明,升降散在内外伤杂病治疗方面应用广泛。根据升降散主要药效成分中浓度前20位的化合物得到的作用靶点525个,与胃炎相关靶点相交集的129个;GO分析得条目数2127条,KEGG分析得通路140条;说明升降散具有多靶点多通路改善胃黏膜损伤的可能性。HE染色结果显示,升降散确能改善大鼠胃黏膜炎症。ELISA实验结果显示,升降散可有效下调大鼠血清中的TNF-α和IL-1β。IHC实验结果表明,升降散有效下调MUC5AC的表达、上调MUC6的表达。结论升降散能以缓解炎症、调节表面黏液屏障的方式改善胃黏膜损伤。

关键词：升降散慢性胃炎文献计量学网络药理学胃组织结构炎症因子免疫组织化学

一种网络化协同工作组系统实时数据分布式动态路由方法

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计算机工程与应用 2004年第24期40卷 120-122,133页

作者：鄢萍刘飞易润忠李建坤重庆大学制造工程研究所重庆400030

文章在分析了现有网络化协同工作组系统数据交互方式存在不足的基础上,提出了一种网络化协同工作组系统实时数据分布式动态路由的方法,并就此方法的组网能力、优化方法、可靠性、网络延迟、负荷均衡等问题进行了分析。

关键词：协同工作组实时数据动态路由分布式

Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： Lan Zhang Jingge Wei Xilei liu Dai Li Xiaoqi Pang Fanglian Chen Hailong Cao ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

枯草芽孢杆菌分泌表达极耐热木聚糖酶及其酶学性质

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江苏农业学报 2016年第5期32卷 1037-1042页

作者：向亚萍罗楚平周华飞刘永锋陈志谊江苏省农业科学院植物保护研究所江苏南京210014 南京农业大学植物保护学院江苏南京210095 淮阴工学院生化学院江苏淮安223001

为将海栖热袍菌极耐热木聚糖酶基因xyn B（Gen Bank登录号AY340789）在枯草芽孢杆菌Bs916中进行异源分泌表达,并阐明工程菌分泌重组木聚糖酶的酶学性质。克隆极耐热木聚糖酶基因xynB,将其与罗克霉素启动子融合,构建融合质粒载体pXYNB2,... 详细信息

为将海栖热袍菌极耐热木聚糖酶基因xyn B（Gen Bank登录号AY340789）在枯草芽孢杆菌Bs916中进行异源分泌表达,并阐明工程菌分泌重组木聚糖酶的酶学性质。克隆极耐热木聚糖酶基因xynB,将其与罗克霉素启动子融合,构建融合质粒载体pXYNB2,将其转化到枯草芽孢杆菌Bs916中,获得重组枯草芽孢杆菌（Bs916/p XYNB2）,并对重组芽孢杆菌分泌表达的极耐热木聚糖酶的酶学性质进行了研究。SDS-PAGE法测定重组枯草芽孢杆菌分泌的蛋白在相对分子质量43 000处有明显的蛋白表达条带。重组木聚糖酶的最适反应p H值为5.0-5.8,重组木聚糖酶的最适反应温度大于或等于100℃,重组极耐热木聚糖酶在pH值4.6-7.8比较稳定,重组木聚糖酶的温度稳定性在90℃下保温2 h后残存酶活83%。可见,生防枯草芽孢杆菌Bs916能有效分泌表达极耐热木聚糖酶,为以后构建多种纤维素和半纤维素在其中的协同表达,拓宽其碳源利用范围奠定基础。

关键词：重组枯草芽孢杆菌海栖热袍菌极耐热木聚糖酶基因融合分泌表达

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PCR-DGGE分析西藏雪莲菌细菌多样性

现代食品科技 2017年第2期33卷 83-88页

作者：王若宇杜丽平刘非马立娟郭学武工业微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院天津300457

细菌是雪莲菌中的重要功能菌群,对于雪莲菌的形成及其功能作用的发挥有着重要作用,因而探明其细菌的种群特征具有重大意义。本文采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和16S r DNA测序技术对3个西藏雪莲菌颗粒(A、B和C)中细菌... 详细信息

细菌是雪莲菌中的重要功能菌群,对于雪莲菌的形成及其功能作用的发挥有着重要作用,因而探明其细菌的种群特征具有重大意义。本文采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和16S r DNA测序技术对3个西藏雪莲菌颗粒(A、B和C)中细菌多样性进行了研究。DGGE电泳结果显示,样品的丰度值介于12~16之间,细菌多样性指数在1.79~2.11之间,相似性指数在0.62~0.77之间。通过对DGGE指纹图谱优势条带切胶测序结果显示:它们分别属于马乳酒样乳杆菌(Lactobacillus kefiranofaciens)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、嗜热链球菌(Streptococcus thermophilus)、开菲尔乳杆菌(Lactobacillus kefir)、气单胞菌属(Aeromonas)和醋酸菌属(Acetobacter)。旨在为雪莲菌微生物资源开发以及加快雪莲菌工业化生产奠定基础。

关键词：雪莲菌聚合酶链式反应变性梯度凝胶电泳细菌多样性