检索结果-南通市图书馆

Encouraging Solution to the Problem of Synthesizing Protein α-Thioester

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2024年第10期42卷 1114-1120页

作者： Xinliang Liu Zijun Gao Jie Zhao Farong Ye ping Huang ping wang Center for Chemical Glycobiology Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsFrontiers Science Center for Transformative MoleculesSchool of Chemistry and Chemical EngineeringZhangjiang Institute for Advanced StudyShanghai JiaoTongUniversityShanghai 200240China Shenzhen Research Institute of Shanghai Jiao Tong University ShenzhenGuangdong 518057China

Expressed protein ligation(EPL)provides a powerful tool to access large-size proteins with precise *** methods for constructing the critical protein thioester for EPL have predominantly relied on the recombinant intein fusion expressed in Escherichia coli(***).Despite its powerful applications,the expression of thioester derived from eukaryotic protein in *** inherently suffers from its limited solubility,the inactivity of intein,premature hydrolysis and low *** overcome these obstacles,we present herein the facile one-flask synthesis of inaccessible proteinα-thioester via a SUMO-protein-intein(SPI)sandwich *** utility of SUMO enhances the protein fusion yield and solubility,prevents premature hydrolysis and simplifies the purification *** inaccessible protein thioester with internal Cys residues can be readily produced and is compatible with the EPL-desulfurization protocol used to prepare complex proteins,which is otherwise difficult to obtain using traditional *** utility has been highlighted through the synthesis of human granulocyte colony-stimulating factor(G-CSF).

关键词： Desulfurization Glycoproteins Glycopeptides Protein expression Peptide thioester

Targeting CD47-SIRPα axis for Hodgkin and non-Hodgkin lymphoma immunotherapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第1期11卷 205-217页

作者： Pengcheng Zhao Longyan Xie Lei Yu ping wang School of Life Sciences and Medicine Shandong University of TechnologyZiboShandong 255000China Tongji University Cancer Center Shanghai Tenth People's HospitalSchool of MedicineTongji UniversityShanghai 200092China

The interaction between cluster of differentiation 47(CD47)and signal regulatory proteinα(SiRPa)protects healthy cells from macrophage attack,which is crucial for maintain-ing immune *** of CD47 occurs widely across various tumor cell types and transmits the"don't eat me"signal to macrophages to avoid phagocytosis through binding to *** of the CD47-SIRPa axis is therefore a promising approach for cancer *** is the most common hematological malignancy and is an area of unmet clin-ical *** review mainly described the current strategies targeting the CD47-SIRPa axis,including antibodies,SiRPaFc fusion proteins,small molecule inhibitors,and peptides both in preclinical studies and clinical trials with Hodgkin lymphoma and non-Hodgkin lymphoma.

关键词： Cancer treatment CD47-SIRPαaxis Hodgkin lymphoma Immunotherapy Non-Hodgkin lymphoma

A method for support stiffness failure identification in a steel spring floating slab track of urban railway: a case study in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第3期25卷 206-222页

作者： Junyuan ZHENG Caiyou ZHAO Duojia SHI ping wang Jian wang Bolong JIANG Xi SHENG Key Laboratoryof High-speed Railway Engineering Ministry of EducationSouthwest Jiaotong UniversityChengdu 60031China School of Civil Engineering Southwest Jiaotong UniversityChengdu 610031China 01 DYNA(Qingdao)Test&Data Co. Ltd.Qingdao 266000China Rail Transit Digital Construction and Measurement Technology National Engineering Research Center China Railway Design CorporationTianjin300308China Institute of Urban Smart Transportation&Safety Maintenance Shenzhen UniversityShenzhen 518060China

The extensive use of steel spring floating slab tracks has effectively addressed the challenge of alleviating the environmental vibrations induced by urban rail transit ***,under the combined action of train dynamic loads and complex environmental factors,problems,such as the fracture of steel spring vibration isolators and suspension vibrations induced by the uneven settlement of the base,often *** failure of isolator support stiffness is often hidden in its early stages and is challenging to identify by conventional detection *** the same time,it will aggravate the wheel-rail interaction,accelerate the deterioration of track structure,and even affect the driving *** study first establishes a detailed coupled train-floating slab track-foundation analytical *** the influence of the vibration isolator support stiffness failure on the dynamic indices of the floating slab track system response is analyzed.A set of defect identification methods that can detect the number of failed steel springs,severity of damage,and their location is ***,an intelligent monitoring system for support stiffness of floating slab track is built by combining the density-based spatial clustering of applications with noise algorithm and statistical data analysis and is applied to a rail line in southern *** a three-year monitoring campaign,a suspension failure and a fracture of a steel spring were each successfully detected and detailed failure information was *** investigation results were consistent with the damage identification *** repair,the track structure dynamic response returned to the average pre-damage level and further deterioration had been *** proposed damage identification methods and monitoring system provide an approach for intelligent identification of track structure support stiffness failures.

关键词： Floating slab track Support stiffness Detailed analytical model Failure identification Monitoring system

A low-profile metamaterial absorber with ultrawideband reflectionless and wide-angular stability

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2024年第4期34卷 258-268页

作者： Feihong Lin Zhongming Yan ping wang Yu wang Hongcheng Zhou Haoran Lu School of Electrical Engineering Southwest Jiaotong UniversityChengdu 611756China Key Laboratory of Magnetic Suspension Technology and Maglev Vehicle Ministry of EducationChengdu 610031China China Academy of Launch Vehicle Technology Beijing 100076China

An ultrawideband reflectionless metamaterial absorber(MA)is proposed by replacing the metallic ground with the complementary split-ring resonator(CSRR)*** proposed MA exhibits-10 d B reflectivity spectrum from 1 GHz to 20 GHz,which maintains more than 90%absorption from 1.5 GHz to20 ***,it achieves angle stability for TE and TM polarization at oblique incident angles up to 40°and 65°,*** achieve broadband absorption spectrum,we have adopted a single-layer high-impedance surface(HIS)loaded with a double-layer magnetic material(MM)*** further realize the RCS reduction into a lower frequency range,we have employed the scattering cancellation technology into the traditional metallic ***,we have fabricated a sample exhibiting the 10 d B RCS reduction from 1 GHz to 20 GHz with a thickness of 10 *** and simulation results confirm that the proposed MA exhibits excellent comprehensive performance,making it suitable for many practical applications.

关键词： Metamaterial absorber(MA) Magnetic material(MM) High-impedance surface(HIS) Scattering cancellation technology Ultrawideband Wide-angular stable

Tensile strength behavior of cement-stabilized dredged sediment reinforced by polypropylene fiber

在线全文

学校读者我要写书评

暂无评论

Frontiers of Structural and Civil Engineering 2024年第3期18卷 380-392页

作者： Lei LANG Jiangshan LI Xin CHEN Lijun HAN ping wang State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicChinese Academy of SciencesWuhan 430071China Hubei Provincial Key Laboratory of Contaminated Sludge and Soil Science and Engineering Wuhan 430071China University of Chinese Academy of Sciences Beijing 100049China

This study evaluated the feasibility of using polypropylene fiber(PF)as reinforcement in improving tensile strength behavior of cement-stabilized dredged sediment(CDS).The effects of cement content,water content,PF content and length on the tensile strength and stress–strain behavioral evolutions were evaluated by conducting splitting tensile strength ***,the micro-mechanisms characterizing the tensile strength behavior inside PF-reinforced CDS(CPFDS)were clarified via analyzing macro failure and microstructure *** results indicate that the highest tensile strengths of 7,28,60,and 90 d CPFDS were reached at PF contents of 0.6%,1.0%,1.0%,and 1.0%,exhibiting values 5.96%,65.16%,34.10%,and 35.83%higher than those of CDS,***,3 mm,PF of showed the best reinforcement *** CPFDS exhibited obvious tensile strain-hardening characteristic,and also had better ductility than *** mix factor(C_(C)^(a)/C_(w)^(b))and time parameter(q_(t0)(t))of CDS,and the reinforcement index(k_(t-PF))of CPFDS were used to establish the tensile strength prediction models of CDS and CPFDS,considering multiple *** PF“bridge effect”and associated cementation-reinforcement coupling actions inside CPFDS were mainly responsible for tensile strength behavior *** key findings contribute to the use of CPFDS as recycled engineering soils.

关键词： dredged sediment chemical stabilization fiber reinforcement splitting tensile strength micro-mechanisms

Study on the dynamic mechanical properties of winding-block axial structure considering short circuit strain rate effect

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High Voltage 2024年第2期9卷 380-390页

作者： Xinyu wang ping wang Jianghai Geng Zikang Zhang Fangcheng Lü Shuguo Gao Hebei Provincial Key Laboratory of Power Transmission Equipment Security Defense North China Electric Power UniversityBaodingChina State Grid Hebei Electric Power Research Institute ShijiazhuangChina

Transformers may suffer multiple short-circuit impacts during long-term operation,and axial instability is one of the typical types of serious accidents caused by short-circuit *** axial instability form of the winding-block structure is analysed,and the dynamic solution of the winding short-circuit electromagnetic force is obtained by estab-lishing the three-dimensional magnetic-circuit-force multi-physical field coupling simulation *** influence of strain rate on the cushion block constitutive equation is corrected,and the modified model is verified by short-circuit impact test and quasi-static *** research results show that for 110 kV 31.5 MVA transformers,the maximum electromagnetic axial resultant force of winding is 363.16 kN,and the ultimate tilt force is 1214.2 *** pre-tightening force configuration is accordingly recom-mended to range from 363.16 to 608.28 kN,which is narrowed by 18.49%compared with the static calculation method;Meanwhile,adding a logarithmic strain rate correction term to the classical constitutive equation of the cushion block can achieve a good correction of the stress-strain relationship with the coefficient of determination above 0.99,and the cushion block has a larger elastic modulus under high strain rate *** research results provide an important theoretical reference for the axial stability structure of transformers.

关键词： axial correction structure

Research on the Relationship Between Environmental and Economic Coupling Systems in Bohai Bay Area Based on a Vector Autoregression(VAR)Model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2024年第2期23卷 557-566页

作者： CAO Huimin wang ping ZHANG Surong XU Dongpo TIAN Weijun Key Laboratory of Marine Environment and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China Business School Qingdao UniversityQingdao 266100China

This study analyzed the impact of land-based contaminants and tertiary industrial structure on economic development in the selected Bohai Bay area,*** on panel data spanning 2011-2020,a vector autoregressive(VAR)model is used to analyze and forecast the short-run and long-run relationships between three industrial structures,pollutant discharge,and economic *** results showed that the environmental index had a long-term cointegration relationship with the industrial structure economic *** capital chemical oxygen demand(PCOD)and per capita ammonia nitrogen(PNH_(3)N)had a positive impact on delta per capita GDP(dPGDP),while per capita solid waste(PSW),the secondary industry rate(SIR)and delta tertiary industry(dTIR)had a negative impact on *** VAR model under this coupling system had stability and *** impulse response results showed that the short-term effect of the coupling system on dPGDP was basically consistent with the Granger causality test *** addition,variance decomposition was used in this study to predict the long-term impact of the coupling system in the next ten periods(i.e.,ten years).It was found that dTIR had a great impact on dPGDP,with a contribution rate as high as 74.35%in the tenth period,followed by the contribution rate of PCOD up to 3.94%,while the long-term contribution rates of PSW,SIR and PNH3N were all less than 1%.The results show that the government should support the development of the tertiary industry to maintain the vitality of economic development and prevent environmental deterioration.

关键词： Bohai Bay area environmental pollution industrial structure cointegration theory VAR model impulse response

Dynamic Response of A Group of Cylindrical Storage Tanks with Baffles Considering the Effect of Soil Foundation

在线全文

学校读者我要写书评

暂无评论

China Ocean Engineering 2024年第1期38卷 129-143页

作者： SUN Ying wang Jia-dong HUO Rui-li ZHOU Ding GU Zhen-yuan QIAN wang-ping School of Transportation and Civil Engineering Nantong UniversityNantong 226019China Faculty of Civil Engineering and Mechanics Jiangsu UniversityZhenjiang 212013China College of Civil Engineering Nanjing Tech UniversityNanjing 211816China

The sloshing in a group of rigid cylindrical tanks with baffles and on soil foundation under horizontal excitation is studied *** solutions for the velocity potential are derived out by the liquid subdomain *** models with mass-spring oscillators are established to replace continuous *** with the least square technique,Chebyshev polynomials are employed to fit horizontal,rocking and horizontal-rocking coupling impedances of soil,respectively.A lumped parameter model for impedance is presented to describe the effects of soil on tank structures.A mechanical model for the soil-foundation-tank-liquid-baffle system with small amount of calculation and high accuracy is proposed using the substructure *** analytical solutions are in comparison with data from reported literature and numerical codes to validate the effectiveness and correctness of the *** dynamic properties and seismic responses of the soil-tank system are given for the baffle number,size and location as well as soil parameter.

关键词： cylindrical tanks multiple annular baffles equivalent analytical model soil−structure interaction subdomain method dynamic response

Emerging mechanisms and implications of c GAS-STING signaling in cancer immunotherapy strategies

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2024年第1期21卷 45-64页

作者： Jiawen Zhang Sihui Yu Qiao Peng ping wang Lan Fang Tongji University Cancer Center Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200072China Reproductive Medicine Center Department of Obstetrics and GynecologyShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China Department of Obstetrics and Gynecology Zhongshan HospitalFudan UniversityShanghai 200032China

The intricate interplay between the human immune system and cancer development underscores the central role of immunotherapy in cancer *** this landscape,the innate immune system,a critical sentinel protecting against tumor incursion,is a key *** cyclic GMP-AMP synthase(c GAS)and stimulator of interferon genes(STING)pathway has been found to be a linchpin of innate immunity:activation of this signaling pathway orchestrates the production of type I interferon(IFN-α/β),thus fostering the maturation,differentiation,and mobilization of immune effectors in the tumor ***,STING activation facilitates the release and presentation of tumor antigens,and therefore is an attractive target for cancer *** strategies to activate the STING pathway,including use of pharmacological agonists,have made substantial advancements,particularly when combined with immune checkpoint *** approaches have shown promise in preclinical and clinical settings,by enhancing patient survival *** review describes the evolving understanding of the c GAS-STING pathway's involvement in tumor biology and ***,this review explores classical and non-classical STING agonists,providing insights into their mechanisms of action and potential for optimizing immunotherapy *** challenges and complexities,the c GAS-STING pathway,a promising avenue for enhancing cancer treatment efficacy,has the potential to revolutionize patient outcomes.

关键词： cGAS-STING pathway type I interferon cyclic dinucleotide STING agonist cancer immunotherapy