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Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella

Insect Science 2023年第4期30卷 1035-1045页

作者： shao-ping Chen Dan-Feng Wang Wei-Feng Ma Xiao-Lu Lin Guang Yang State Key Laboratory of Ecological Pest ControlforFujan and Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhouChina Joint International Research Laboratory of Ecological Pest Control Ministry of EducationFuzhouChina Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops Fujian Agriculture and Forestry UniversityFuzhouChina Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of AgricultureFuzhouChina Key Laboratory of Green Pest Control(Fujian Agriculture and Forestry University) Fujian Province UniversityFuzhouChina

Cryptochrome 1(CRY1)functions as a light-responsive photoreceptor,which is crucial for circadian *** identity and function of CRY1 in Plutella xylostella remain *** this study,cryl was cloned and identified in P ***,a cry1-knockout strain(Cry1-KO)of P xylostella with a 2-bp deletion was established from the strain Geneva 88(G88)using the CRISPR/Cas9 *** daily temporal os-cillation of cryl was observed in G88 and Cry1-KO,and cryl mean daily transcription of Cry1-KO was lower than that of *** G88 and Cry1-KO demonstrated rhythmic locomotion under the light/dark condition with Cryl-KO being more active than G88 in the daytime,whereas Cry1-KO completely lost rhythmicity under constant *** developmental period of pre-adult of Cry1-KO was longer than that of G88;the lifespan of the Cry1-KO male adult was shorter than that of G88;the fecundity of Cry1-KO was lower than that of G88;and Cry1-KO showed lower intrinsic rate of increase(r),net repro-duction rate(Ro),finite increase rate(a),and longer mean generation time(T)than *** results indicate that cryl is involved in the regulation of locomotor circadian rhythm and development in P xylostella,providing a potential target gene for controlling the pest and a basis for further investigation on circadian rhythms in lepidopterans.

关键词： circadian rhythm cryptochrome 1 lifespan locomotor activity Plutella xylostella reproduction

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Fluorescence spectra of colloidal self-assembled CdSe nano-wire on substrate of porous Al2O3/Au nanoparticles

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Chinese Physics B 2019年第6期28卷 429-432页

作者： Xin Zhang Li-ping shao Man Peng Zhong-Chen Bai Zheng-ping Zhang Shui-Jie Qin College of Big Data and Information Engineering Guizhou UniversityGuiyang 550025China College of Medicine Guizhou UniversityGuiyang 550025China Guizhou Province Key Laboratory for Photoelectric Technology and Application Guizhou UniversityGuiyang 550025China

We present a self-assembly method to prepare array nano-wires of colloidal CdSe quantum dots on a substrate of porous Al2 O3 film modified by gold nanoparticles. The photoluminescence(PL) spectra of nanowires are in situ measured by using a scanning near-field optical microscopy(SNOM) probe tip with 100-nm aperture on the scanning near-field optical microscope. The results show that the binding sites from the edge of porous Al2 O3 nanopores are combined with the carboxyl of CdSe quantum dots’ surface to form an array of CdSe nanowires in the process of losing background solvent because of the gold nanoparticles filling the nano-holes of porous Al2 O3 film. Compared with the area of nonself-assembled nano-wire, the fluorescence on the Al2 O3/Au/CdSe interface is significantly enhanced in the self-assembly nano-wire regions due to the electron transfer conductor effect of the gold nanoparticles’ surface. In addition, its full width at half maximum(FWHM) is also obviously widened. The method of enhancing fluorescence and energy transfer can widely be applied to photodetector, photocatalysis, optical display, optical sensing, and biomedical imaging, and so on.

关键词： enhanced photoluminescence Cd Se nano-wire colloidal self-assembled method porous Al2O3 film

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OPTIMAL COARSE GRID SIZE IN DOMAIN DECOMPOSITION

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Journal of Computational Mathematics 1994年第4期12卷 291-297页

作者： Tony Chan Jian-ping shao(Department of Mathematics, University of Califoruia, Los Angeles, USA) UNIV CALIF LOS ANGELES DEPT MATHLOS ANGELESCA 90024

In most domain decomposition (DD) methods, a coarse grid solve is employed to provide the global coupling required to produce an optimal method. The total cost of a method can depend sensitively on the choice of the coaxse grid size H. In this paper, we give a simple analysis of this phenomenon for a model elliptic problem and a variant of Smith's vertex space domain decomposition method [11, 3]. We derive the optimal value Hopt which asymptotically minimises the total cost of method (number of floating point operations in the sequential case and execution time in the parallel case), for subdomain solvers with different complekities. Using the value of Hopt, we derive the overall complexity of the DD method, which can be significantly lower than that of the subdomain solver

关键词： UCLA OPTIMAL COARSE GRID SIZE IN DOMAIN DECOMPOSITION CAM

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Photooxidation stability of phytosterols with different relative spatial positions in different particles

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Food Innovation and Advances 2023年第3期2卷 225-232页

作者： Jingjian Liu Dan Wang ping shao Simin Feng Department of Food Science and Engineering Zhejiang University of TechnologyHangzhou 310014ZhejiangChina Key Laboratory of Food Macromolecular Resources Processing Technology Research(Zhejiang University of Technology) China National Light IndustryHangzhou 310014China

The aim of this study was to investigate the effects of relative spatial position of stigmasterol on its photooxidation stability in different *** oxidation products(POPs)from phytosterol oxidation were successfully isolated and studied using solid phase extraction(SPE)technology in conjunction with *** photooxidation stability of stigmasterol in four particles was as follows:zein stabilized particles(ZPs)≈zein-pectin stabilized particles(ZPPs)>soy protein isolate(SPI)-pectin stabilized particles(SPPs)>SPI stabilized particles(SPs).7β-Hydroxy and 5β,6β-epoxy was the main POPs in the first and second oxidation stages,respectively,which reached 8,945±43μg/g and 6,010±289μg/g after 240 min UV light exposure treatment in *** stigmasterol was hydrophobically adsorbed on the surface of SPs,the network gel generated by pectin outside SPPs prevented photooxidation of *** stigmasterol was encapsulated in the interior of ZPs,the blocking effect of pectin in ZPPs became *** study provided a feasible development direction for the storage and quality control of phytosterols as dietary supplements.

关键词： stability photooxidation stabilized

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EXPERIMENTAL STUDY OF VELOCITY DISTRIBUTIONS IN THE TRANSITION REGION OF PIPES

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Journal of Hydrodynamics 2011年第5期23卷 643-648页

作者： LIU Yong-hui DU Guang-sheng LIU Li-ping shao Zhu-feng ZHAI Cheng-yuan School of Energy and Power Engineering -Shandong University Jinan 250061 China

The accuracy of an ultrasonic flowmeter meaurement depends on the profile-linear average velocity. But this velocity in the transition region is not available at the present. In this article, the velocity in the transition region in pipes is studied by experimental methods. The Particle Image Velocimetry （ PIV ） is used to measure the flow field in the transition region in pipes, and the measured results from PIV are in good agreement with the Westerwell＇s experimental data. Based on the experimental data of PIV, the curves of the profile-linear average velocity in the transition region against the Reynolds number in the range from 2 000 to 20 000 are obtained, and it is shown that the coefficient k is constant when the Reynolds number is in the range of 2 000-2 400 and 6 000-20 000, and the coefficient k is increasing when the Reynolds number is in the range of 2 400-6 000. The results of this article can be used to improve the measurement accuracy of the ultrasonic flowmeters and as a theoretical basis for the research on the transition flow.

关键词： ultrasonic flowmeter Particle Image Velocimetry (PIV) the profile-linear average velocity transition region pipe

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3D computational modeling and perceptual analysis of kinetic depth effects

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Computational Visual Media 2020年第3期6卷 265-277页

作者： Meng-Yao Cui shao-ping Lu Miao Wang Yong-Liang Yang Yu-Kun Lai Paul L.Rosin TKLNDST CSNankai UniversityTianjinChina State Key Laboratory of Virtual Reality Technology and Systems Beihang UniversityBeijingChina Department of Computer Science University of BathUK School of Computer Science and Informatics Cardiff UniversityWalesUK

Humans have the ability to perceive kinetic depth effects, i.e., to perceived 3 D shapes from 2 D projections of rotating 3 D objects. This process is based on a variety of visual cues such as lighting and shading effects. However, when such cues are weak or missing, perception can become faulty, as demonstrated by the famous silhouette illusion example of the spinning dancer. Inspired by this, we establish objective and subjective evaluation models of rotated3 D objects by taking their projected 2 D images as input. We investigate five different cues: ambient luminance, shading, rotation speed, perspective, and color difference between the objects and *** the objective evaluation model, we first apply3 D reconstruction algorithms to obtain an objective reconstruction quality metric, and then use quadratic stepwise regression analysis to determine weights of depth cues to represent the reconstruction quality. In the subjective evaluation model, we use a comprehensive user study to reveal correlations with reaction time and accuracy, rotation speed, and perspective. The two evaluation models are generally consistent, and potentially of benefit to inter-disciplinary research into visual perception and 3 D reconstruction.

关键词： rotation spinning rotating

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Postconditioning of sevoflurane and propofol is associated with mitochondrial permeability transition pore

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第2期9卷 100-108页

作者： Wei HE Feng-jiang ZHANG shao-ping WANG Gang CHEN Cong-cong CHEN Min YAN Department of Anesthesiology the SecondAffiliatedHospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Cardiology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Background: Sevoflurane and propofol are effective cardioprotective anaesthetic agents, though the cardioprotection of propofol has not been shown in humans. Their roles and underlying mechanisms in anesthetic postconditioning are unclear. Mitochondrial permeability transition pore (MPTP) opening is a major cause of ischemia-reperfusion injury. Here we investigated sevoflurane- and propofol-induced postconditioning and their relationship with MPTP. Methods: Isolated perfused rat hearts were exposed to 40 min of ischemia followed by 1 h of reperfusion. During the first 15 min of reperfusion, hearts were treated with either control buffer (CTRL group) or buffer containing 20 μmol/L atractyloside (ATR group), 3% (v/v) sevoflurane (SPC group), 50 μmol/L propofol (PPC group), or the combination of atractyloside with respective anesthetics (SPC+ATR and PPC+ATR groups). Infarct size was determined by dividing the total necrotic area of the left ventricle by the total left ventricular slice area (percent necrotic area). Results: Hearts treated with sevoflurane or propofol showed significantly better recovery of coronary flow, end-diastolic pressures, left ventricular developed pressure and derivatives compared with controls. Sevoflurane resulted in more protective alteration of hemodynamics at most time point of reperfusion than propofol. These improvements were paralleled with the reduction of lactate dehydrogenase release and the decrease of infarct size (SPC vs CTRL: (17.48±2.70)% vs (48.47±6.03)%, P<0.05; PPC vs CTRL: (35.60±2.10)% vs (48.47±6.03)%, P<0.05). SPC group had less infarct size than PPC group (SPC vs PPC: (17.48±2.70)% vs (35.60±2.10)%, P<0.05). Atractyloside coadministration attenuated or completely blocked the cardioprotective effect of postconditioning of sevoflurane and propofol. Conclusion: Postconditioning of sevoflurane and propofol has cardio-protective effect against ischemia-reperfusion injury of heart, which is associated with inhibition of MPTP o

关键词： Sevoflurane Propofol Postconditioning Reperfusion injury Mitochondrial permeability transition pore (MPTP)

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Wave Propagation in Accretion Disks with Self-Gravity

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Chinese Physics Letters 2001年第2期18卷 301-303页

作者： LIU Xiao-Ci YANG Lan-Tian WU shao-ping DING Shi-Xue Department of Physics and Institute of AstrophysicsHuazhong Normal UniversityWuhan 430079 Department of Physics Hainan Normal CollegeHaikou 571158 Department of Physics Xiangfan Normal CollegeXiangfan 441053

We extend the research by Lubow and Pringle of axisymmetric waves in accretion disks to the case where self-gravity of disks should be *** derive and analyse the dispersion relations with the effect of *** show that self-gravity extends the forbidden region of the wave propagation:for high frequency p-modes,self-gravity makes the wavelength shorter and the group velocity larger;for low frequency g-modes,the effect is opposite.

关键词： accretion effect shorter

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Population Inversion of a V-type Three-Level Atom due to the Effect of Cavity-Induced Quantum Interference

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Chinese Physics Letters 2001年第1期18卷 48-50页

作者： LI Gao-Xiang PENG Jin-Sheng WU shao-ping HUANG Guang-Ming Department of Physics Huazhong Normal UniversityWuhan 430079

A cavity-modified master equation is derived for a coherently driven V-type three-level atom coupled to a singlemode cavity in the bad cavity *** influences of the cavity-induced quantum interference on the steady-state populations are *** show that the population inversion can occur due to the effect of cavity-induced quantum interference.

关键词： quantum Inversion coherent

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Resource reduction for simultaneous generation of two types of continuous variable nonclassical states

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Frontiers of physics 2021年第2期16卷 3-8页

作者： Long Tian shao-ping Shi Yu-Hang Tian Ya-Jun Wang Yao-Hui Zheng Kun-Chi Peng State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

We demonstrate experimentally the simultaneous generation and detection of two types of continuous variable nonclassical states from one type-0 phase-matching optical parametric armplification(OPA)and subsequent two ring filter cavities(RFCs).The output field of the OPA includes the baseband wo and sideband modes ω0±nωf subjects to the cavity resonance condition,which are separated by two cascaded *** first RFC resonates with half the pump wavelength wo and the transmitted baseband component is a squeezed *** relcted fields of the first RFC,including the sideband modes ω0±wf,are separated by the second RFC,construct Einstein Podolsky-Rosen entangled *** freedoms,including the filter cavities for sideband separation and relative phases for the measure-ments of these sidebands,are actively *** noise variance of squeezed states is 10.2 dB below the shot noise limit(SNL),the correlation variances of both quadrature amplitude-sum and quadrature phase diference for the entanglement state are 10.0 dB below the corresponding SNL.

关键词： squeezed states entanglement states continuous variable non-classical states

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