Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella

作     者：Shao-Ping Chen Dan-Feng Wang Wei-Feng Ma Xiao-Lu Lin Guang Yang 

作者机构：State Key Laboratory of Ecological Pest ControlforFujan and Taiwan CropsInstitute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhouChina Joint International Research Laboratory of Ecological Pest ControlMinistry of EducationFuzhouChina Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait CropsFujian Agriculture and Forestry UniversityFuzhouChina Key Laboratory of Integrated Pest Management for Fujian-Taiwan CropsMinistry of AgricultureFuzhouChina Key Laboratory of Green Pest Control(Fujian Agriculture and Forestry University)Fujian Province UniversityFuzhouChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2023年第30卷第4期

页      面：1035-1045页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：funded by the National Key R&D Program of China,grant number 2017YFD0200400 the Special Key Project of Fujian Province,grant number 2018NZ01010013,and a fund of"111"program,grant numberKRA16001A 

主　　题：circadian rhythm cryptochrome 1 lifespan locomotor activity Plutella xylostella reproduction 

摘      要：Cryptochrome 1(CRY1)functions as a light-responsive photoreceptor,which is crucial for circadian *** identity and function of CRY1 in Plutella xylostella remain *** this study,cryl was cloned and identified in P ***,a cry1-knockout strain(Cry1-KO)of P xylostella with a 2-bp deletion was established from the strain Geneva 88(G88)using the CRISPR/Cas9 *** daily temporal os-cillation of cryl was observed in G88 and Cry1-KO,and cryl mean daily transcription of Cry1-KO was lower than that of *** G88 and Cry1-KO demonstrated rhythmic locomotion under the light/dark condition with Cryl-KO being more active than G88 in the daytime,whereas Cry1-KO completely lost rhythmicity under constant *** developmental period of pre-adult of Cry1-KO was longer than that of G88;the lifespan of the Cry1-KO male adult was shorter than that of G88;the fecundity of Cry1-KO was lower than that of G88;and Cry1-KO showed lower intrinsic rate of increase(r),net repro-duction rate(Ro),finite increase rate(a),and longer mean generation time(T)than *** results indicate that cryl is involved in the regulation of locomotor circadian rhythm and development in P xylostella,providing a potential target gene for controlling the pest and a basis for further investigation on circadian rhythms in lepidopterans.