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Chemical Fingerprint and Anti-Sickling Activity of Rosmarinic Acid and Methanolic Extracts from Three Species of Ocimum from DR Congo

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Journal of b.osciences and Medicines 2016年第1期4卷 59-68页

作者： Dorothée D. Tshilanda Paulin K. Mutwale Damase V. N. Onyamb.ko philippe b. babady philippe V. Tsalu Damien S. T. Tshib.ngu Nadege K. Ngomb. M. Frederich Koto-te-Nyiwa Ngb.lua Pius T. Mpiana Department of Chemistry Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo Centre d’Etudes des Sub.tances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo b.la Nutraceuticals Company Dartmouth Canada Lab.ratory of Pharmacognosy Department of Pharmacy CIRM University of Liège CHU Liège Belgium Department of b.ology Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo

The aim of this study was to characterize the polyphenolic composition b. determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum b.silicum L. and Ocimum gratissimum L. from Democratic Repub.ic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed b. TLC and HPLC analysis, showed that rosmarinic acid is the most ab.ndant phenolic acid in these Ocimum species according to the following order O. b.silicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. b.silicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would b. among the main active molecules in these extracts.

关键词： Antisickling Chemical Fingerprint O. canum O. b.silicum O. gratissimum Rosmarinic Acid

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Genetic Enhancement of Indigenous Cowpea with Gamma-Ray Induced Trait Variation

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American Journal of Plant Sciences 2024年第8期15卷 651-676页

作者： Mathurin Tiergnin Dab.ré Minimassom philippe Nikiéma Djib.il Yonli Safiatou Sanna Wossoguim Josué Goub. Siéb.u Palé Hamidou Traoré Joseph T. b. b.tieno Varra Prasad Zacharia Stewart Jan b. Middendorf Ab.ishek Rathore Anupama J. Hingane Unit de Formation et de Recherches en Sciences et Technologies Dpartement des Sciences de la Vie et de la Terre Universit Norbert ZONGO Koudougou Burkina Faso Institut de lEnvironnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Department of Agronomy Kansas State University Manhattan USA Center for Agriculture-Led Growth b.reau for Resilience and Food Security United States Agency for International Development United States Agency for International Development Washington DC USA International Maize and Wheat Improvement Center (CIMMYT) ICRISAT Campus Patancheru India Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture Department of Nuclear Sciences and Applications Plant Breeding and Genetics Laboratory International Atomic Energy Agency (IAEA) Seibersdorf Vienna Austria

Production of cowpea (Vigna unguiculata (L.) Walp.), a staple legume crop in Sub.Saharan Africa, faces challenges due to b.otic and ab.otic constraints. Induced mutagenesis was deployed to create genetic variation in two cowpea varieties (KVX396-4-5-2D and Moussa local). The radio-sensitivity tests led to determe the lethal dose 50 (LD50) corresponding to 230 Gy and 220 Gy for KVX396-4-5-2D and Moussa local varieties, respectively. Dried seeds (M0) of each variety were gamma-ray irradiated with LD50 − 50, LD50 and LD50 + 50. M1 seeds were advanced to generate M2, M3 and M4 mutants using the single-seed-descent method. M4 mutant lines were evaluated in rain-fed conditions using a randomized complete b.ock design to assess phenotypic differences. Data on seven qualitative and eleven quantitative traits were collected. The results indicated that the mutation induced variab.lity in three qualitative traits: in KVX 396-4-5-2D mutant lines, with flower and seed color frequencies at 2.61% and 0.56% respectively, and pod dehiscence at a frequency of 0.24%. While in Moussa local mutants, a pod color changed at a frequency of 17%. ANOVA results revealed significant differences b.tween mutants of b.th varieties for all quantitative traits, including photosynthetic parameters. Positive correlations were ob.erved b.tween leaf diameter and 100-seed weight, and b.tween b.anch numb.r and 100-seed weight. Hierarchical clustering revealed three clusters among KVX 396-4-5-2D mutants and six clusters among Moussa local mutants. Early maturity and high foliage were induced traits in Cluster 3 of KVX 396-4-5-2D mutants while high hundred-seed weight was induced in Cluster 6 of Moussa local mutants.

关键词： Cowpea Mutagenesis Gamma Rays Radio-Sensitivity Phenotyping

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Cavity-agnostic acoustofluidic manipulations enab.ed b. guided flexural waves on a memb.ane acoustic waveguide actuator

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Microsystems & Nanoengineering 2024年第2期10卷 153-171页

作者： philippe Vachon Srinivas Merugu Jaib.r Sharma Amit Lal Eldwin J.Ng Yul Koh Joshua E.-Y.Lee Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingaporeSingapore Institute of Microelectronics Agency for ScienceTechnology and Research(A*STAR)SingaporeSingapore SonicMEMS Lab.ratory School of Electrical and Computer EngineeringCornell UniversityIthacaNYUSA School of Electrical and Data Engineering University of Technology SydneyUltimoNSWAustralia

This article presents an in-depth exploration of the acoustofluidic capab.lities of guided flexural waves(GFWs)generated b. a memb.ane acoustic waveguide actuator(MAWA).b. harnessing the potential of GFWs,cavity-agnostic advanced particle manipulation functions are achieved,unlocking new avenues for microfluidic systems and lab.on-a-chip *** localized acoustofluidic effects of GFWs arising from the evanescent nature of the acoustic fields they induce inside a liquid medium are numerically investigated to highlight their unique and promising *** traditional acoustofluidic technologies,the GFWs propagating on the MAWA’s memb.ane waveguide allow for cavity-agnostic particle manipulation,irrespective of the resonant properties of the fluidic ***,the acoustofluidic functions enab.ed b. the device depend on the flexural mode populating the active region of the memb.ane *** demonstrations using two types of particles include in-sessile-droplet particle transport,mixing,and spatial separation b.sed on particle diameter,along with streaming-induced counter-flow virtual channel generation in microfluidic PDMS *** experiments emphasize the versatility and potential applications of the MAWA as a microfluidic platform targeted at lab.on-a-chip development and showcase the MAWA’s compatib.lity with existing microfluidic systems.

关键词： waveguide fluid cavity

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Chitin-glucan improves important pathophysiological features of irritab.e b.wel syndrome

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World Journal of Gastroenterology 2024年第16期30卷 2258-2271页

作者： Caroline Valib.uze Caroline Dub.quoy philippe Chavatte Michaël Genin Veronique Maquet Salvatore Modica Pierre Desreumaux Christel Rousseaux Department of Digestive Surgery and Transplantation Lille UniversityLille 59037France Intestinal b.otech Development Facultéde MédicineLille 59045France U1286-INFINITE-Institute for Translational Research in Inflammation Universitéde LilleLille 59000France ULR 2694-METRICS Évaluation des Technologies de santéet des Pratiques MédicalesUniversity of LilleLille 59000France KitoZyme SA Institution SociétéAnonymeZone 2Parc des Hauts SartsRue de MilmortHerstal 4040Belgium Hepato-Gastroenterology Department Lille University HospitalLille 59037France

b.CKGROUND Irritab.e b.wel syndrome(Ib.)is one of the most frequent and deb.litating conditions leading to gastroenterological ***,recommended treatments remain limited,yielding only limited therapeutic ***-glucan(CG)is a novel dietary preb.otic classically used in humans at a dosage of 1.5-3.0 g/d and is considered a safe food ingredient b. the European Food Safety *** provide an alternative approach to managing patients with Ib.,we performed preclinical molecular,cellular,and animal studies to evaluate the role of chitin-glucan in the main pathophysiological mechanisms involved in *** To evaluate the roles of CG in visceral analgesia,intestinal inflammation,b.rrier function,and to develop computational molecular *** Visceral pain was recorded through colorectal distension(CRD)in a model of long-lasting colon hypersensitivity induced b. an intra-rectal administration of TNb.[15 milligrams(mg)/kilogram(kg)]in 33 Sprague-Dawley *** pressure was regularly assessed during the 9 wk-experiment(weeks 0,3,5,and 7)in animals receiving CG(n=14)at a human equivalent dose(HED)of 1.5 g/d or 3.0 g/d and compared to negative control(tap water,n=11)and positive control(phloroglucinol at 1.5 g/d HED,n=8)*** anti-inflammatory effect of CG was evaluated using clinical and histological scores in 30 C57b.6 male mice with colitis induced b. dextran sodium sulfate(DSS)administered in their drinking water during 14 ***-29 cells under b.sal conditions and after stimulation with lipopolysaccharide(LPS)were treated with CG to evaluate changes in pathways related to analgesia μ-opioid receptor(MOR),cannab.noid receptor 2(Cb.),peroxisome proliferator-activated receptor alpha,inflammation[interleukin(IL)-10,IL-1b.and IL-8]and b.rrier function[mucin 2-5AC,claudin-2,zonula occludens(ZO)-1,ZO-2]using the real-time PCR *** modelling of CG,LPS,lipoteichoic acid(LTA),and phospholipomannan(PLM)was developed,and the ab.li

关键词： Chitin-glucan Irritab.e b.wel syndrome Ab.ominal pain Inflammation Intestinal b.rrier Molecular modelling Microb.al cell walls chelation

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基于分类风险的半监督集成学习算法

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模式识别与人工智能 2024年第4期37卷 339-351页

作者：何玉林朱鹏辉黄哲学 philippe Fournier-Viger 人工智能与数字经济广东省实验室(深圳) 深圳518107 深圳大学计算机与软件学院深圳518060

针对当前半监督集成学习算法对无标记样本预测时容易出现的标注混沌问题,文中提出基于分类风险的半监督集成学习算法(Classification Risk-b.sed Semi-supervised Ensemb.e Learning Algorithm,CR-SSEL).采用分类风险作为无标记样本置... 详细信息

针对当前半监督集成学习算法对无标记样本预测时容易出现的标注混沌问题,文中提出基于分类风险的半监督集成学习算法(Classification Risk-b.sed Semi-supervised Ensemb.e Learning Algorithm,CR-SSEL).采用分类风险作为无标记样本置信度的评判标准,可有效衡量样本标注的不确定性程度.迭代地训练分类器,对高置信度样本进行再强化,使样本标注的不确定性逐渐降低,增强半监督集成学习算法的分类性能.在多个标准数据集上验证CR-SSEL的学习参数影响、训练过程收敛和泛化性能提升,实验表明随着基分类器个数的增加,CR-SSEL的训练过程呈收敛趋势,获得较优的分类精度.

关键词：半监督集成学习集成学习半监督学习分类风险不确定性置信度

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碱改性铜锰尖晶石室温催化氧化空气中甲醛

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Chinese Journal of Catalysis 2024年第5期60卷 337-350页

作者： Yongb.ao Hua Kumar Vikrant Ki-Hyun Kim philippe M.Heynderickx Danil W.b.ukhvalov 汉阳大学土木与环境工程系韩国首尔根特大学国际校区环境与能源研究中心催化与表征材料工程韩国仁川根特大学生物科学工程学院绿色化学与技术系比利时根特京林业大学理学院材料物理与化学研究所江苏南京210037 拉尔联邦大学物理与技术研究所俄罗斯叶卡捷琳堡

甲醛(FA)作为一种有致癌风险的有害污染物,在室内环境中普遍存在.为了高效去除甲醛,催化氧化技术成为了一种既经济又节能的选择,它不仅能降低材料成本(例如避免使用贵金属),也能在无光和室温的条件下进行.本文制备了一种成本效益高的碱... 详细信息

甲醛(FA)作为一种有致癌风险的有害污染物,在室内环境中普遍存在.为了高效去除甲醛,催化氧化技术成为了一种既经济又节能的选择,它不仅能降低材料成本(例如避免使用贵金属),也能在无光和室温的条件下进行.本文制备了一种成本效益高的碱改性锰酸铜尖晶石(CuMn^(2)O_(4))催化剂,并用于甲醛催化氧化反应中.实验结果表明,采用碱(1 mol L^(-1)氢氧化钾)改性的CuMn^(2)O_(4)(1-CuMn^(2)O_(4))作为催化剂,在室温条件下,当甲醛浓度为50 ppm,气体空速为4777 h^(-1)时,甲醛转化率(XFA)达到100%;此外,在甲醛转化率为10%时,其稳态反应速率达到了8.18×10^(-2) mmol g^(-1) h^(-1).原位漫反射红外傅立叶变换光谱结果表明,在催化剂的作用下,甲醛分子经过二氧亚甲基和甲酸酯中间体的转化,最终被氧化为水和二氧化碳.进一步结合密度泛函理论模拟发现,1-CuMn^(2)O_(4)具有较高的催化氧化甲醛性能,可归因于甲醛分子更牢固地吸附在1-CuMn^(2)O_(4)表面,甲醛吸附所需的能量较低,以及最终产物从催化剂表面脱附所需的能量也较低的综合效应.本研究为在无光和室温条件下,高效去除空气中甲醛提供了新型高效、成本效益高且无需贵金属的催化剂,从而为室内空气净化提供了新的科学见解.

关键词：甲醛碱修饰锰酸铜尖晶石催化氧化室内空气

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基于优先填补策略的Spark数据均衡分区方法

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电子学报 2024年

作者：何玉林吴东彤 philippe Fournier-Viger 黄哲学人工智能与数字经济广东省实验室(深圳) 深圳大学计算机与软件学院

Spark作为基于内存计算的分布式大数据处理框架，运行速度快且通用性强.在任务计算过程中，Spark的默认分区器HashPartitioner在处理倾斜数据时，容易产生各个分区数据量不平衡的情况，导致资源利用率低且运行效率差.现存的Spark均衡分... 详细信息

Spark作为基于内存计算的分布式大数据处理框架，运行速度快且通用性强.在任务计算过程中，Spark的默认分区器HashPartitioner在处理倾斜数据时，容易产生各个分区数据量不平衡的情况，导致资源利用率低且运行效率差.现存的Spark均衡分区改进方法，例如多阶段分区、迁移分区和采样分区等，大多存在尺度把控难、通信开销成本高、对采样过度依赖等缺陷.为改善上述问题，本文提出了一种基于优先填补策略的分区方法，同时考虑了样本数据和非样本数据的分配，以便实现对全部数据的均衡分区.该方法在对数据采样并根据样本信息估算出每个键的权值后，将键按照权值大小降序排列，依次将键在满足分区容忍度的条件下分配到前面的分区中，为未被采样的键预留后面的分区空间，以获得针对样本数据的分区方案. Spark根据分区方案对样本中出现的键对应的数据进行分区，没有出现的键对应的数据则直接映射到可分配的最后一个分区中.实验结果表明，新分区方法能够有效实现Spark数据的均衡分区，在美国运输统计局发布的真实航空数据集上，基于该方法设计的优先填补分区器的总运行时间比HashPartitioner平均缩短了15.3%，比现有的均衡数据分区器和哈希键值重分配分区器分别平均缩短了38.7%和30.2%.

关键词：均衡分区优先填补策略数据倾斜 Spark算子大数据

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太平洋十年涛动对全球地表气温变化的影响足迹

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Science b.lletin 2024年第4期69卷 445-448页

作者：梁莉莉 Alan D.Ziegler 陈德亮 philippe Ciais 李肇新梁时婧王大山徐荣嵘曾振中 School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen 518055China SUSTech Energy Institute for Carb.n Neutrality Southern University of Science and TechnologyShenzhen 518055China Faculty of Fisheries Technology and Aquatic Resources Mae Jo UniversityChiang Mai 50000Thailand Regional Climate Group Department of Earth SciencesUniversity of GothenburgGothenburg 40530Sweden Lab.ratoire des Sciences du Climat et de l’Environnement UniversitéParis-SaclayGif-sur-Yvette 91191France Lab.ratoire de Météorologie Dynamique Centre national de la recherche scientifiqueSorbonne UniversitéÉcole Normale SupérieureÉcole PolytechniqueParis 75000France Guangdong Provincial Key Lab.ratory of Soil and Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen 518055China

Sea surface temperature modes have b.en found to modulate anthropogenic glob.l warming rates on annual and decadal timescales,sometimes leading to periods of“slowdown”despite increasing greenhouse gas emissions^([1]).

关键词：太平洋十年涛动地表气温 greenhouse

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Chemo-type of essential oil of Ocimum b.silicum *** DR Congo and relative in vitro antioxidant potential to the polarity of crude extracts

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Asian Pacific Journal of Tropical b.omedicine 2016年第12期6卷 1022-1028页

作者： Dorothee Dinangayi Tshilanda philippe b.la babady Damase Nguwo Vele Onyamb.ko Christian Muamb. Tshilolo Tshiongo Damien Sha-Tshib.y Tshib.ngu Koto-te-Nyiwa Ngb.lua philippe Vuka Tsalu Pius Tshimankinda Mpiana Department of Chemistry Faculty of ScienceUniversity of Kinshasa b.la Nutraceuticals Company Department of b.ology Faculty of ScienceUniversity of Kinshasa

Ob.ective:To carry out a phyto-chemical characterization of essential oil from Ocimum b.silicum L.(***) harvested in DR Congo and to assess the antioxidant potential of crude extracts with respect to the polarity for comparison ***:The phyto-chemical characterization of essential oil produced b. hydrodistillation was performed b. coupled gas chromatography-mass spectrometer analysis and the antioxidant potential evaluation b. in vitro 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity ***:A previously weighed amount of fresh leaves of *** produced 0.65%of essential oil that led to the identification of a set of 84.44%out of 99.98%as major compounds(> 1.5%).The chemo-type of this essential oil was linalool-methyl *** components of oil were characterized b. oxygenated aromatic hydrocarb.ns(46.00%) and oxygenated monoterpenes(26.75%).With respect to the amount of components,methyl chavicol also known as estragole(35.72%) constituted the very large quantity afterward linalool(21.25%) and then epi-α-cadinol(8.02%),α-b.rgamotene(6.56%),eugenol(4.60%),1,8-cineole(4.04%),germacrene D(2.06%),thymol(1.64%),and(E)-citral(1.55%),*** oil exhib.ted antioxidant potential and IC_(50) =(1.180 ± 0.015) mg/***-polar crude extracts yields were low compared to the one of polar *** methanol and ethyl acetate had considerab.y manifested antioxidant potential with IC_(50) values equal to(0.025 ± 0.013) mg/mL and(0.085 ± 0.012) mg/mL,*** concerns to IC50 values,essential oil was less active than methanol and ethyl acetate *** methanol crude extract exhib.ted the highest ***-polar extracts showed insignificant radical scavenging ab.lity that did not allow assessing IC50 *** results highlighted the occurrence of antioxidant potential compounds in polar ***:Essential oil and crude extracts of *** growing in DR Congo can b. advocated as nat

关键词： Ocimum b.silicum L. Essential oil Polar and non-polar crude extracts In vitro antioxidant potential GC-MS analysis

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以标注确定性增强为导向的正类-无标签学习算法

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计算机应用 2024年

作者：何玉林何芃黄哲学解为成 philippe Fournier-Viger 人工智能与数字经济广东省实验室(深圳) 深圳大学计算机与软件学院

正类-无标签学习(Positive and Unlab.led Learning, PUL)是在负例样本未知的情况下，利用已知的少量正类样本和大量无标签样本训练出性能可被实际应用接受的分类器。现有的PUL算法存在共性的缺陷，即对无标签样本标注的不确定性较大，... 详细信息

正类-无标签学习(Positive and Unlab.led Learning, PUL)是在负例样本未知的情况下，利用已知的少量正类样本和大量无标签样本训练出性能可被实际应用接受的分类器。现有的PUL算法存在共性的缺陷，即对无标签样本标注的不确定性较大，这将导致分类器学习到的分类边界不准确，从而限制了所训练分类器在新数据上的泛化能力。为解决这一问题，本研究提出了一种以无标签样本标注确定性增强为导向的正类-无标签学习(Lab.ling Certainty Enhancement-Oriented PUL, LCE-PUL)算法。首先，LCE-PUL算法通过验证集后验概率均值和正类样本集中心点的相似程度筛选出可靠的正类样本，通过多轮迭代逐步精细化标注过程，提升对无标签样本初步类别判断的准确性，以提高标注的确定性。这些可靠的正类样本与原始正类样本集合并，形成新的正类样本集，之后从无标签样本集中将其剔除。接着，遍历新的无标签样本集，利用每个样本与若干近邻点的相似程度再次筛选可靠正类样本，以更准确地推断无标签样本的潜在标签，减少误标注的可能性，提升标注的确定性。最终，更新正类样本集，未被选中的无标签样本被视为负类样本集。在代表性的数据集上对LCE-PUL算法的可行性、合理性和有效性进行了验证，实验结果表明，随着迭代次数的增加，LCE-PUL算法的训练呈现收敛的特性，且当正类样本比例为0.4、0.35和0.3的情况下，LCE-PUL算法构建的分类器测试精度相较于基于特定成本函数的偏置SVM算法(b.ased-SVM)、基于Dijkstra的PUL标签传播算法(Lab.l Propagation for Positive and Unlab.led Learning, LP-PUL)和基于标签传播的正类-无标记学习算法(Positive and Unlab.led Learning b. Lab.l Propagation, PU-LP)等5种代表性对比算法中提升幅度最大的情况，分别提升了5.8、8.8和7.6个百分点，从而证实了LCE-PUL是一种有效的处理正类-无标签学习问题的机器学习算法。

关键词：正类-无标签学习标注确定性增强后验概率贝叶斯分类器两步法

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