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Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

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Journal of Innovative Optical Health Sciences 2018年第5期11卷 59-68页

作者： Hengchang Guo Hsing-Wen Wang Qinggong Tang Erik Anderson Reuben Falola Tikina Smith Yi Liu moshe Levi peter m.andrews Yu Chen Fischell Department of Bioengineering University of maryland College Park MD 20742USA Department of Biochemistry and molecular&Cellular Biology Georgetown University medical Center Washington DC 20007 USA Central Animal Resources Facility University of maryland College ParkMD 20742USA

Adriamycin(doxorubicin),a common cancer chemotherapeutic drug,can be used to induce a model of chronic progressive glomerular disease in *** our studies,we evahuated renal changes in a rat model after Adriamydin injection using two photon microscopy(TPm),optical coherence tomography(OCT)and Doppler OCT(DOCT).Taking advantage of deep penetra-tion and fast scanning speed for three dimensional(3D)label-free imaging,OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real *** imaging renal pathology following the infusion of fAuorophore-labeled dextrans of different molecular weights,TPm can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal ***,glomerular permeability and filtration,proximal and distal tubular flow dynamics can be revealed.6-8 weeks after injection of Adriamycin,TPm and OCT/DOCT imaging revealed glomerular sclerosis,compromised flow across the glomerular wall,tubular atrophy,tubular dilation,and variable intra-tubular flow *** results indicate that TPm and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures.

关键词： Chronic kidney disease(CKD) glomeruloscerosis Optical Coherence Tomography(OCT) Tubular Atrophy Two-photon microscopy(TPm)

Assessment of buckling-restrained braced frame reliability using an experimental limit-state model and stochastic dynamic analysis

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Earthquake Engineering and Engineering Vibration 2009年第3期8卷 373-385页

作者： Blake m.andrews Junho Song Larry A.Fahnestock Wiss JanneyElstner AssociatesIncNorthbrookIllinoisUSA Department of Civil and Environmental Engineering University of Illinois at Urbana-ChampaignUrbanaIllinoisUSA

Buckling-restrained braces （BRBs） have recently become popular in the United States for use as primary members of seismic lateral-force-resisting systems. A BRB is a steel brace that does not buckle in compression but instead yields in both tension and compression. Although design guidelines for BRB applications have been developed, systematic procedures for assessing performance and quantifying reliability are still needed. This paper presents an analytical framework for assessing buckling-restrained braced frame （BRBF） reliability when subjected to seismic loads. This framework efficiently quantifies the risk of BRB failure due to low-cycle fatigue fracture of the BRB core. The procedure includes a series of components that：（1） quantify BRB demand in terms of BRB core deformation histories generated through stochastic dynamic analyses; （2） quantify the limit-state of a BRB in terms of its remaining cumulative plastic ductility capacity based on an experimental database; and （3） evaluate the probability of BRB failure, given the quantified demand and capacity, through structural reliability analyses. Parametric studies were conducted to investigate the effects of the seismic load, and characteristics of the BRB and BRBF on the probability of brace failure. In addition, fragility curves （i.e., conditional probabilities of brace failure given ground shaking intensity parameters） were created by the proposed framework. While the framework presented in this paper is applied to the assessment of BRBFs, the modular nature of the framework components allows for application to other structural components and systems.

关键词： risk and reliability analysis buckling-restrained brace stochastic dynamic analysis first-order reliability method cumulative plastic ductility capacity

Doctor communication quality and Friends' attitudes influence complementary medicine use in inflammatory bowel disease

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World Journal of Gastroenterology 2015年第12期21卷 3663-3670页

作者： Réme mountifield Jane m.andrews Antonina mikocka-Walus peter Bampton Department of Gastro-enterology and Hepatology Flinders Medical Centre Flinders University of South Australia IBD Service Department of Gastroenterology and Hepatology and School of Medicine University of Adelaide at Royal Adelaide Hospital School of Nursing and midwifery University of South Australia

AIm: To examine the frequency of regular complementary and alternative therapy(CAm) use in three Australian cohorts of contrasting care setting and geography, and identify independent attitudinal and psychological predictors of CAm use across all cohorts. mETHODS: A cross sectional questionnaire was administered to inflammatory bowel disease(IBD) patients in 3 separate cohorts which differed by geographical region and care setting. Demographics and frequency of regular CAm use were assessed, along with attitudes towards IBD medication and psychological parameters such as anxiety, depression, personality traits and quality of life(QOL), and compared across cohorts. Independent attitudinal and psychological predictors of CAm use were determined using binary logistic regression analysis. RESULTS: In 473 respondents(mean age 50.3 years, 60.2% female) regular CAm use was reported by45.4%, and did not vary between cohorts. Only 54.1%of users disclosed CAm use to their doctor. Independent predictors of CAm use which confirm those reported previously were: covert conventional medication dose reduction(P ment(P medication(P = 0.043), and higher QOL(P m use by family or friends(P mmunication(P m use, these data show that physician attention to communication and the patient-doctor relationship is important as these factors influence CAm use. Patient reluctance to discuss CAm with physicians may promote greater reliance on social contacts to influence CAm decisions.

关键词： Complementary medicine Alternative therapy Therapy

Human pluripotent stem cells:tools for regenerative medicine

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Biomaterials Translational 2021年第4期2卷 294-300页

作者： peter W.andrews The Centre for Stem Cell Biology The School of BioscienceThe University of SheffieldSheffieldUK

Human embryonic stem cells and induced pluripotent stem cells,together denoted as pluripotent stem cells have opened up unprecedented opportunities for developments in human healthcare over the past 20 *** much about the properties and behaviour of these cells required to underpin their applications has been discovered over this time,a number of issues *** brief review considers the history of these developments and some of the underlying biology,pointing out some of the problems still to be resolved,particularly in relation to their genetic stability and possible malignancy.

关键词： embryonic stem cell induced pluripotent stem cell human pluripotency differentiation

Outcomes and patients' perspectives of transition from paediatric to adult care in inflammatory bowel disease

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World Journal of Gastroenterology 2016年第8期22卷 2611-2620页

作者： Alice L Bennett David moore peter A Bampton Robert V Bryant Jane m.andrews Department of Gastroenterology Royal Adelaide Hospital North Terrace Adelaide SA 5000 Australia Department of Gastroenterology Flinders Medical Centre Flinders Drive Bedford Park SA 5042 Australia School of medicine University of Adelaide Adelaide SA 5000 Australia Department of Gastroenterology Women's and Children's Hospital Adelaide SA 5006 Australia School of medicine Flinders University Adelaide SA 5001 Australia

AIm: To describe the disease and psychosocial outcomes of an inflammatory bowel disease (IBD) transition cohort and their ***: Patients with IBD, aged > 18 years, who had moved from paediatric to adult care within 10 years were identified through IBD databases at three tertiary hospitals. Participants were surveyed regarding demographic and disease specific data and their perspectives on the transition process. Survey response data were compared to contemporaneously recorded information in paediatric service case notes. Data were compared to a similar age cohort who had never received paediatric IBD care and therefore who had not undergone a transition ***: There were 81 returned surveys from 46 transition and 35 non-transition patients. No statistically significant differences were found in disease burden, disease outcomes or adult roles and responsibilities between cohorts. Despite a high prevalence of mood disturbance (35%), there was a very low usage (5%) of psychological services in both cohorts. In the transition cohort, knowledge of their transition plan was reported by only 25/46 patients and the majority (54%) felt they were not strongly prepared. A high rate (78%) of discussion about work/study plans was recorded prior to transition, but a near complete absence of discussion regarding sex (8%), and other adult issues was recorded. Both cohorts agreed that their preferred method of future transition practices (of the options offered) was a shared clinic appointment with all key ***: Transition did not appear to adversely affect disease or psychosocial outcomes. Current transition care processes could be optimised, with better psychosocial preparation and agreed transition plans.

关键词： Transition care Crohn’ s disease Ulcerative colitis Chronic illness Inflammatory bowel disease Patient perspectives Disease outcomes

An empirical movement model for sixgill sharks in Puget Sound： Combining observed and unobserved behavior

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Current Zoology 2012年第1期58卷 103-115页

作者： Levin, Phillip S. Horne, peter andrews, Kelly S. Williams, Greg Northwest Fisheries Science Center 2725 Montlake Blvd E Seattle WA 98112 USA

Understanding the movement of animals is fundamental to population and community ecology. Historically, it has been difficult to quantify movement patterns of most fishes, but technological advances in acoustic telemetry have increased our abilities to monitor their movement. In this study, we combined small-scale active acoustic tracking with large-scale passive acoustic monitoring to develop an empirical movement model for sixgill sharks in Puget Sound, WA, USA. We began by testing whether a correlated random walk model described the daily movement of sixgills; however, the model failed to capture home-ranging behavior. We added this behavior and used the resultant model （a biased random walk model） to determine whether daily movement patterns are able to explain large-scale seasonal movement. The daily model did not explain the larger-scale pat- terns of movement observed in the passive monitoring data. In order to create the large-scale patterns, sixgills must have per- formed behaviors （large, fast directed movements） that were unobserved during small-scale active tracking. In addition, seasonal shifts in location were not captured by the dally model. We added these ＇unobserved＇ behaviors to the model and were able to capture large-scale seasonal movement of sixgill sharks over 150 days. The development of empirical models of movement al- lows researchers to develop hypotheses and test mechanisms responsible for a species movement behavior and spatial distribution. This knowledge will increase our ability to successfully manage species of concern [Current Zoology 58 （1）： 103-115, 2012].

关键词： Hexanchus griseus movement Correlated random walk Biased random walk Displacement movement model

Aneuploidy in pluripotent stem cells and implications for cancerous transformation

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Protein & Cell 2014年第8期5卷 569-579页

作者： Jie Na Duncan Baker Jing Zhang peter W. andrews Ivana Barbaric School of medicine Tsinghua University Beijing 100084 China Sheffield Diagnostic Genetic Services Sheffield Children's Hospital Sheffield S10 2TH UK Centre for Stem Cell Biology Department of Biomedical Science The University of Sheffield Sheffield S10 2TN UK

Owing to a unique set of attributes, human pluripotent stem cells （hPSCs） have emerged as a promising cell source for regenerative medicine, disease modeling and drug discovery. Assurance of genetic stability over long term maintenance of hPSCs is pivotal in this endeavor, but hPSCs can adapt to life in culture by acquiring non-random genetic changes that render them more robust and easier to grow. In separate studies between 12.5% and 34% of hPSC lines were found to acquire chromosome abnormalities over time, with the incidence increasing with passage number. The predominant genetic changes found in hPSC lines involve changes in chromosome number and structure （particularly of chromosomes 1, 12, 17 and 20）, remi- niscent of the changes observed in cancer cells. In this review, we summarize current knowledge on the causes and consequences of aneuploidy in hPSCs and highlight the potential links with genetic changes observed in human cancers and early embryos. We point to the need for comprehensive characterization of mechanisms underpinning both the acquisition of chromosomal abnormalities and selection pressures, which allow mutations to persist in hPSC cultures. Elucidation of these mechanisms will help to design culture conditions that minimize the appearance of aneuploid hPSCs. moreover, aneuploidy in hPSCs may provide a unique platform to analyse the driving for- ces behind the genome evolution that may eventually lead to cancerous transformation.

关键词： human pluripotent stem cells (hPSCs),culture adaptation aneuploidy cancer genetic changes

Comparison of three-dimensional fluorescence analysis methods for predicting formation of trihalomethanes and haloacetic acids

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Journal of Environmental Sciences 2015年第1期27卷 159-167页

作者： Nicolás m.Peleato Robert C.andrews Department of Civil Engineering University of Toronto

This work investigated the application of several fluorescence excitation–emission matrix analysis methods as natural organic matter（NOm） indicators for use in predicting the formation of trihalomethanes（THms） and haloacetic acids（HAAs）. Waters from four different sources（two rivers and two lakes） were subjected to jar testing followed by 24 hr disinfection by-product formation tests using chlorine. NOm was quantified using three common measures： dissolved organic carbon, ultraviolet absorbance at 254 nm, and specific ultraviolet absorbance as well as by principal component analysis, peak picking,and parallel factor analysis of fluorescence spectra. Based on multi-linear modeling of THms and HAAs, principle component（PC） scores resulted in the lowest mean squared prediction error of cross-folded test sets（THms： 43.7（μg/L）^2, HAAs： 233.3（μg/L）^2）. Inclusion of principle components representative of protein-like material significantly decreased prediction error for both THms and HAAs. Parallel factor analysis did not identify a protein-like component and resulted in prediction errors similar to traditional NOm surrogates as well as fluorescence peak picking. These results support the value of fluorescence excitation–emission matrix–principal component analysis as a suitable NOm indicator in predicting the formation of THms and HAAs for the water sources studied.

关键词： Natural organic matter Fluorescence Principal component analysis PARAFAC Disinfection byproducts Drinking water

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