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Human immune cells＇ behavior and survival under bioenergetically restricted conditions in an in vitro fracture hematoma model

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Cellular & Molecular Immunology 2013年第2期10卷 151-158页

作者： Paula Hoff patrick Maschmeyer Timo Gaber Tabea Schiitze Tobias Raue Katharina Schmidt-Bleek Rene Dziurla Saskia Schellmann Ferenz Leonard Lohanatha Eric Rohner Andrea Ode Gerd-Riidiger Burmester Georg N Duda Carsten Perka Frank Buttgereit Department of Rheumatelogy and Clinical Immunology Charite University Hospital Berlin Germany German Arthritis Research Center (DRFZ) Berlin Germany berlin-Brandenburg Center for Regenerative Therapies (BCRT) Berlin Germany Julius Wolff Institute Berlin Germany Department of Orthopedics Charite University Hospital Berlin Germany These authors contributed equally to this work

The initial inflammatory phase of bone fracture healing represents a critical step for the outcome of the healing process. However, both the mechanisms initiating this inflammatory phase and the function of immune cells present at the fracture site are poorly understood. In order to study the early events within a fracture hematoma, we established an in vitro fracture hematoma model： we cultured hematomas forming during an osteotomy （artificial bone fracture） of the femur during total hip arthroplasty （THA） in vitro under bioenergetically controlled conditions. This model allowed us to monitor immune cell populations, cell survival and cytokine expression during the early phase following a fracture. Moreover, this model enabled us to change the bioenergetical conditions in order to mimic the in vivo situation, which is assumed to be characterized by hypoxia and restricted amounts of nutrients. Using this model, we found that immune cells adapt to hypoxia via the expression of angiogenic factors, chemoattractants and pro-inflammatory molecules. In addition, combined restriction of oxygen and nutrient supply enhanced the selective survival of lymphocytes in comparison with that of myeloid derived cells （i.e., neutrophils）. Of note, non-restricted bioenergetical conditions did not show any similar effects regarding cytokine expression and/or different survival rates of immune cell subsets. In conclusion, we found that the bioenergetical conditions are among the crucial factors inducing the initial inflammatory phase of fracture healing and are thus a critical step for influencing survival and function of immune cells in the early fracture hematoma.

关键词： apoptosis fracture hematoma model hypoxia immune cells inflammation

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Biodegradable magnesium fixation screw for barrier membranes used in guided bone regeneration

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Bioactive Materials 2022年第8期7卷 15-30页

作者： Zeljka Peric Kacarevic patrick Rider Akiva Elad Drazen Tadic Daniel Rothamel Gerrit Sauer Fabien Bornert Peter Windisch David Botond Hangyasi Balint Molnar Till Kammerer Bernhard Hesse Emely Bortel Marco Bartosch Frank Witte Department of Anatomy Histology EmbryologyPathology Anatomy and Pathology HistologyFaculty of Dental Medicine and HealthUniversity of OsijekOsijek31000Croatia Department of Prosthodontics Geriatric Dentistry and Craniomandibular DisordersCharite-Universitatsmedizin BerlinAßmannshauser Straße 4–614197BerlinGermany Botiss Biomaterials AG Ullsteinstrasse 10812109BerlinGermany CMF Surgery Johannes BLA HospitalMonchengladbachGermany Dental Clinic University of StrasbourgFrance Department of Periodontology Semmelweis UniversityBudapestHungary Xploraytion GmbH Bismarkstrasse 11BerlinGermany University Hospital Munich Department of Dermatology and AllergyFrauenlobstr.9-1180337MunichGermany Biotrics Bioimplants AG Ullsteinstrasse 10812109BerlinGermany

An ideal fixation system for guided bone(GBR)regeneration in oral surgery must fulfil several criteria that includes the provision of adequate mechanical fixation,complete resorption when no longer needed,complete replacement by bone,as well as be biocompatible and have a good clinical *** the first time,a biodegradable magnesium fixation screw made of the magnesium alloy WZM211 with a MgF2 coating has been designed and tested to fulfill these *** mechanical fixation was shown for the magnesium fixation screw in several benchtop tests that directly compared the magnesium fixation screw with an equivalent polymeric resorbable *** demonstrated slightly superior mechanical properties of the magnesium device in comparison to the polymeric device even after 4 weeks of *** of the magnesium fixation screw was demonstrated in several in vitro and in vivo *** of the magnesium screw was investigated in in vitro and in vivo tests,where it was found that the screw is resorbed slowly and completely after 52 weeks,providing adequate fixation in the early critical healing ***,the magnesium fixation screw demonstrates all of the key properties required for an ideal fixation screw of membranes used in guided bone regeneration(GBR)surgeries.

关键词： Magnesium Biodegradable Implant GBR Bone healing Soft tissue healing

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高性能混凝土中的微裂纹

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商品混凝土 2007年第6期 60-66页

作者： Udo Wiens Birgit Meng patrick fontana 孙振平同济大学材料科学与工程学院

正如人们对高性能混凝土中微裂纹对其耐久性影响方面的认识程度一样,人们至今对低水灰比混凝土内部微裂纹产生原因的认识仍然很浅。可以设想,微裂纹是由于人们要获得高强而通常采用的硅灰所引起的特殊反应特性而导致的。这里,Aachen的R... 详细信息

正如人们对高性能混凝土中微裂纹对其耐久性影响方面的认识程度一样,人们至今对低水灰比混凝土内部微裂纹产生原因的认识仍然很浅。可以设想,微裂纹是由于人们要获得高强而通常采用的硅灰所引起的特殊反应特性而导致的。这里,Aachen的RWTH建筑研究所(ibac)开展了有关火山灰活性掺合料早期的反应机理以及活性掺合料对混凝土孔溶液中碱浓度随时间变化的影响。联邦材料研究和测试所(BAM)就硅灰对水泥浆体早期的自收缩、水化动力学和内部组织特性等方面开展的研究,为本项工作进行了必要的补充。

关键词：高性能混凝土微裂纹水泥浆体硅灰自收缩活性掺合料冻融循环混凝土试件火山灰活性混凝土结

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Reduced anoctamin 7(ANO7) expression is a strong and independent predictor of poor prognosis in prostate cancer

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Cancer Biology & Medicine 2021年第1期18卷 245-255页

作者： Andreas Marx Lena Koopmann Doris Hoflmayer Franziska Büscheck Claudia Hube-Magg Stefan Steurer Till Eichenauer Till S.Clauditz Waldemar Wilczak Ronald Simon Guido Sauter Jakob R.Izbicki Hartwig Huland Hans Heinzer Markus Graefen Alexander Haese Thorsten Schlomm Christian Bernreuther patrick Lebok Sarah Bonk Institute of Pathology Klinikum FurthFurth 90766Germany Institute of Pathology University Medical Center Hamburg-EppendorfHamburg 20246Germany Department of Urology University Medical Center Hamburg-EppendorfHamburg 20246Germany General Visceral and Thoracic Surgery Department and ClinicUniversity Medical Center Hamburg-EppendorfHamburg 20246Germany Martini-Clinic Prostate Cancer CenterUniversity Medical Center Hamburg-EppendorfHamburg 20246Germany Department of Urology Charité-Universit?tsmedizin BerlinBerlin 10117Germany

Objective:Anoctamin 7(ANO7)is a calcium2+-dependent chloride ion channel *** expression is restricted to prostate epithelial *** exact function is *** study aimed to analyze ANO7 expression and its clinical significance in prostate cancer(PCa).Methods:ANO7 expression was assessed by immunohistochemistry in 17,747 clinical PCa ***:ANO7 was strongly expressed in normal prostate glandular cells but often less abundant in cancer ***7 staining was interpretable in 13,594 cancer tissues and considered strong in 34.4%,moderate in 48.7%,weak in 9.3%,and negative in 7.6%.Reduced staining was tightly linked to adverse tumor features[high classical and quantitative Gleason grade,lymph node metastasis,advanced tumor stage,high Ki67 labeling index,positive surgical margin,and early biochemical recurrence(P<0.0001 each)].The univariate Cox hazard ratio for prostate-specific antigen(PSA)recurrence after prostatectomy in patients with negative *** ANO7 expression was 2.98(95%confidence interval 2.61–3.38).The prognostic impact was independent of established pre-or postoperatively available parameters(P<0.0001).Analysis of annotated molecular data showed that low ANO7 expression was linked to TMPRSS2:ERG fusions(P<0.0001),elevated androgen receptor expression(P<0.0001),as well as presence of 9 of 11 chromosomal deletions(P<0.05 each).A particularly strong association of low ANO7 expression with phosphatase and tensin homolog(PTEN)deletion may indicate a functional relationship with the PTEN/AKT ***:These data identify reduced ANO7 protein expression as a strong and independent predictor of poor prognosis in ***7 measurement,either alone or in combination,might provide clinically useful prognostic information in PCa.

关键词： ANO7 immunohistochemistry prognosis prostate cancer

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Introducing the Open Energy Ontology: Enhancing data interpretation and interfacing in energy systems analysis

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能源与人工智能(英文) 2021年第3期5卷 53-66页

作者： Meisam Booshehri Lukas Emele Simon Flügel Hannah Förster Johannes Frey Ulrich Frey Martin Glauer Janna Hastings Christian Hofmann Carsten Hoyer-Klick Ludwig Hülk Anna Kleinau Kevin Knosala Leander Kotzur patrick Kuckertz Till Mossakowski Christoph Muschner Fabian Neuhaus Michaja Pehl Martin Robinius Vera Sehn Mirjam Stappel Institute for Applied Ecology BerlinGermany Institute for Applied Informatics(InfAI)/Leipzig University Germany Department of Energy Systems Analysis Institute of Networked Energy SystemsGerman Aerospace Center(DLR)StuttgartGermany Faculty of Computer Science Otto-von-Guericke-Universität MagdeburgGermany Reiner Lemoine Institut BerlinGermany Institute of Techno-economic Systems Analysis(IEK-3) Forschungszentrum JülichJülichGermany Institute of Energy Economics and Rational Energy Use University of StuttgartGermany Potsdam Institute for Climate Impact Research PotsdamGermany Fraunhofer Institute for Energy Economics and Energy System Technology KasselGermany

Heterogeneous data,different definitions and incompatible models are a huge problem in many domains,with no exception for the field of energy systems ***,it is hard to re-use results,compare model results or couple models at *** provide a precisely defined vocabulary to build a common and shared conceptu-alisation of the energy ***,we present the Open Energy Ontology(OEO)developed for the domain of energy systems *** the OEO provides several benefits for the ***,it enables consistent annotation of large amounts of data from various research *** example is the Open Energy Platform(OEP).Adding such annotations makes data semantically searchable,exchangeable,re-usable and ***,computational model coupling becomes much *** advantages of using an ontology such as the OEO are demonstrated with three use cases:data representation,data annotation and interface *** also describe how the ontology can be used for linked open data(LOD).

关键词： Collaborative ontology development Linked open data Metadata annotation Energy systems analysis

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Alzheimer’s Disease Aβ-Amyloid Plaque Morphology Varies According to APOE Isotype

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World Journal of Neuroscience 2023年第3期13卷 118-133页

作者： Ina Caesar K. Peter R. Nilsson Per Hammarström Mikael Lindgren Stefan Prokop James Schmeidler Vahram Haroutunian David M. Holtzman patrick R. Hof Sam Gandy Department of Neurology Icahn School of Medicine at Mount Sinai New York NY USA Department of Physics Chemistry and Biology Link&#246ping University Link&#246ping Sweden Department of Physics Norwegian University of Science and Technology Trondheim Norway Department of Neuropathology Charité Universit&#228tsmedizin Berlin Berlin Germany Department of Psychiatry Icahn School of Medicine at Mount Sinai New York NY USA Alzheimer’s Disease Research Center Icahn School of Medicine at Mount Sinai New York NY USA James J. Peters VA Medical Center Bronx NY USA Nash Family Department of Neuroscience and Friedman Brain Institute Icahn School of Medicine at Mount Sinai New York NY USA Department of Neurology Hope Center for Neurological Disorders Knight Alzheimer’s Disease Research Center Washington University St. Louis MO USA Ronald M. Loeb Center for Alzheimer’s Disease Icahn School of Medicine at Mount Sinai New York NY USA

Background: The apolipoprotein E (APOE, gene;apoE, protein) ε4 allele is the most commonly identified genetic risk factor for typical late-onset sporadic Alzheimer’s disease (AD). Each APOE ε4 allele roughly triples the relative risk for AD compared to that of the reference allele, APOE ε3. Methods: We have employed hyperspectral fluorescence imaging with an amyloid-specific, conformation-sensing probe, p-FTAA, to elucidate protein aggregate structure and morphology in fresh frozen prefrontal cortex samples from human postmortem AD brain tissue samples from patients homozygous for either APOE ε3 or APOE ε4. Results: As expected APOE ε4/ε4 tissues had a significantly larger load of CAA than APOE ε3/ε3. APOE isoform-dependent morphological differences in amyloid plaques were also observed. Amyloid plaques in APOE ε3/ε3 tissue had small spherical cores and large coronas while amyloid plaques in APOE ε4/ε4 tissues had large irregular and multi-lobulated plaques with relatively smaller coronas. Despite the different morphologies of their cores, the p-FTAA stained APOE ε3/ε3 amyloid plaque cores had spectral properties identical to those of APOE ε4/ε4 plaque cores. Conclusions: These data support the hypothesis that one mechanism by which the APOE ε4 allele affects AD is by modulating the macrostructure of pathological protein deposits in the brain. APOE ε4 is associated with a higher density of amyloid plaques (as compared to APOE ε3). We speculate that multilobulated APOE ε4-associated plaques arise from multiple initiation foci that coalesce as the plaques grow.

关键词： Alzheimer’s Disease Amyloid Apolipoprotein Luminescent Conjugated Oligothiophenes Hyperspectral Separation

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肿瘤18F-FDG PET/MRI全身显像专家共识(德国)

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中华核医学与分子影像杂志 2020年第9期40卷 549-555页

作者：王洋洋杨光杰(译) 王振光(审校) Lale Umutlu Thomas Beyer Johannes Stefan Grueneisen Christoph Rischpler Harald H Quick Veit-Haibach patrick Matthias Eiber Sandra Purz Gerald Antoch Sergios Gatidis Konstantin Nikolaou Jürgen FSchaefer IvoRausch Herrmann Ken K.Herrmann B.J.Krause S.O.Schoenberg L.Umutlu F.Anton G.Antoch M.Hacker M.Luster S.Neumann D.Vorwerk 青岛大学附属医院核医学科PET/CT中心 266100 Department of Diagnostic and Interventional Radiology and Neuroradiology University-Hospital EssenGermany QIMP Group Centre for Medical Imaging and Biomedical EngineeringMedical University of ViennaAustria Department of Nuclear Medicine University-Hospital EssenGermany High-Field-and Hybrid-MR-Imaging University-Hospital EssenGermany Joint Department of Medical Imaging University of TorontoCanada Department of Nuclear Medicine Klinikum rechts der IsarTechnical University of MunichGermany Department of Nuclear Medicine University of LeipzigGermany Department of Diagnostic and Interventional Radiology University DüsseldorfMedical FacultyDüsseldorfGermany Department of Diagnostic and Interventional Radiology University-Hospital TübingenGermany Department of Nuclear Medicine University Medical Center RostockGermany Department of Clinical Radiology and Nuclear Medicine University Medical Center MannheimGermany Deutsche Röntgengesellschaft BerlinGermany Division of Nuclear Medicine Department of Biomedical Imaging and Image-guided TherapyMedical University ViennaAustria Department of Nuclear Medicine University Hospital MarburgGermany Department of Radiology Klinikum IngolstadtGermany

PET/MRI一体机在2006年被首次提出可应用于临床;2010年,首台临床型PET/MRI一体机诞生。在20世纪早期,PET/CT和SPECT/CT一体机的广泛应用体现了融合显像的优势,为PET/MRI一体机的产生奠定了基础。目前,全球大约有150台全身PET/MRI一体机... 详细信息

PET/MRI一体机在2006年被首次提出可应用于临床;2010年,首台临床型PET/MRI一体机诞生。在20世纪早期,PET/CT和SPECT/CT一体机的广泛应用体现了融合显像的优势,为PET/MRI一体机的产生奠定了基础。目前,全球大约有150台全身PET/MRI一体机投入了临床应用,其中肿瘤显像是PET/MRI的主要应用领域之一。迄今为止,尽管PET/MRI临床应用在增加,但标准化的PET/MRI扫描方案还很少。因此,有必要制定患者检查和多中心研究都可遵循的标准化且一致性好的显像方案。该文总结了18F-脱氧葡萄糖(FDG)PET/MRI全身显像的患者就诊、检查准备、工作流程、显像方案以及报告书写等主要方面的专家共识,由长期使用PET、MRI和较早使用PET/MRI的相关资深专家制定。

关键词： PET/MRI 肿瘤全身显像专家共识

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca Leidy Yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres José Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-Campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-Corredor Jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey Botanical Garden and Botanical Museum berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de Caldas(FAUC) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 Chavis Avenue LexingtonVA 24450USA Charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´Celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de Ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,Soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,Chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&Spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,CabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-Corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia Citizen science Chile Mexico Puerto Rico Sri Lanka

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