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Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells

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Bioactive Materials 2022年第9期7卷 120-130页

作者： Zhule Wang Liebert Parreiras Nogueira Håvard Jostein Haugen Ingrid CM.Van der Geest patricia caetano de almeida rodrigues dennis Janssen Thom Bitter Jeroen J.J.P.van den Beucken Sander CG.Leeuwenburgh Radboud University Medical Center Department of Dentistry-Regenerative BiomaterialsRadboud Institute for Molecular Life SciencesNijmegenthe Netherlands University of Oslo Department of BiomaterialsInstitute of Clinical DentistryFaculty of DentistryOsloNorway Radboud University Medical Center Department of OrthopedicsRadboud Institute for Health SciencesNijmegenthe Netherlands

Malignant bone tumors are usually treated by resection of tumor tissue followed by filling of the bone defect with bone graft ***(PMMA)cement is the most commonly used bone substitute in clinical orthopedics in view of its ***,the dense nature of PMMA renders this biomaterial unsuitable for local delivery of chemotherapeutic drugs to limit the recurrence of bone ***,we introduce porosity into PMMA cement by adding carboxymethylcellulose(CMC)to facilitate such local delivery of chemotherapeutic drugs,while retaining sufficient mechanical properties for bone reconstruction in load-bearing *** results show that the mechanical strength of PMMA-based cements gradually decreases with increasing CMC *** incorporation of≥3%CMC,the PMMA-based cements released up to 18%of the loaded cisplatin,in contrast to cements containing lower amounts of CMC which only released less than 2%of the cisplatin over 28 *** release of cisplatin efficiently killed osteosarcoma cells in vitro and the fraction of dead cells increased to 91.3%at day 7,which confirms the retained chemotherapeutic activity of released cisplatin from these PMMA-based ***,tibias filled with PMMA-based cements containing up to 3%of CMC exhibit comparable compressive strengths as compared to intact *** conclusion,we demonstrate that PMMA cements can be rendered therapeutically active by introducing porosity using CMC to allow for release of cisplatin without compromising mechanical properties beyond critical *** such,these data suggest that our dual-functional PMMA-based cements represent a viable treatment option for filling bone defects after bone tumor resection in load-bearing sites.

关键词： Porous polymethylmethacrylate cement Bone tumor treatment Local drug delivery Chemotherapeutic drug Ex vivo biomechanical assessment

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Role of short chain fatty acids in gut health and possible therapeutic approaches in inflammatory bowel diseases

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World Journal of Clinical Cases 2022年第28期10卷 9985-10003页

作者： Marcos Antônio Ferreira caetano patricia Castelucci department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSão Paulo 05508900SPBrazil

Inflammatory bowel diseases(IBDs) are characterized by inflammation in the gastrointestinal tract and include Ulcerative Colitis and Crohn’s *** diseases are costly to health services,substantially reduce patients’ quality of life,and can lead to complications such as cancer and even *** include abdominal pain,stool bleeding,diarrhea,and weight *** treatment of these diseases is symptomatic,seeking disease *** intestine is colonized by several microorganisms,such as fungi,viruses,and bacteria,which constitute the intestinal microbiota(IM).IM bacteria promotes dietary fibers fermentation and produces short-chain fatty acids(SCFAs) that exert several beneficial effects on intestinal *** can bind to G protein-coupled receptors,such as GPR41 and GPR43,promoting improvements in the intestinal barrier,anti-inflammatory,and antioxidant ***,SCFAs could be a therapeutic tool for ***,the mechanisms involved in these beneficial effects of SCFAs remain poorly ***,this paper aims to provide a review addressing the main aspects of IBDs,and a more detailed sight of SCFAs,focusing on the main effects on different aspects of the intestine with an emphasis on IBDs.

关键词： Ulcerative colitis Crohn’s disease Short-chain fatty acid Butyrate Inflammatory bowel diseases Free fatty acid receptor

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Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes Laura B Maifrino patricia Castelucci Romeu rodrigues de Souza Edson A Liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins Light microscopy Transmission electron microscopy

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Ground peach pits: alternative substrate component for seedling production

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Journal of Forestry Research 2019年第5期30卷 1779-1791页

作者： patricia Mieth Maristela Machado Araujo Maria Helena Fermino Suelen Carpenedo Aimi Daniele rodrigues Gomes Juliana de Marques Vilella department of Forest Sciences Federal University of Santa MariaSanta MariaBrazil FEPAGRO–Agricultural Research Foundation of the State of Rio Grande do Sul Porto AlegreBrazil Federal University of Rio Grande do Sul Porto AlegreBrazil

The exploitation of agro-industrial wastes is a strategy to add value to seedling production and reduce environmental *** production chain for processed peaches generates,approximately,21,000 tons of fruit worldwide,and the pits are used mainly to generate *** objective of the study was to investigate the use of different proportions of ground peach pits(GPP)as a substrate component for seedling growth of Eucalyptus dunnii in the nursery and *** proportions of GPP mixed with either commercial substrate(CS)or with brown peat(BP)were *** physical and chemical properties of the substrates,the morphological and physiological attributes of the seedlings and their correlation with substrate properties were *** GPP increased the density,aeration space and pH and reduced total porosity,liquid retention and electric conductivity of the *** use of this component resulted in hormesis,a biphasic dose–response model in which low levels of exposure to this component stimulated biological performance of plants and high levels of exposure inhibited *** improved seedling growth in the nursery and adequate post-planting development of ***,GPP with CS is recommended at 20%of the total substrate and with BP between 15 and 30%.

关键词： Organic waste Prunus persica Eucalyptus dunnii Tree species Morphological attributes

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Study of tumor necrosis factor receptor in the inflammatory bowel disease

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World Journal of Gastroenterology 2023年第18期29卷 2733-2746页

作者： Roberta Figueiroa Souza Marcos Antônio Ferreira caetano Henrique Inhauser Riceti Magalhães patricia Castelucci department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000Brazil department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil

Ulcerative colitis(UC)and Crohn’s disease(CD)are part of Inflammatory Bowel Diseases(IBD)and have pathophysiological processes such as bowel necrosis and enteric neurons and enteric glial *** addition,the main inflammatory mediator is related to the tumor necrosis factor-alpha(TNF-α).TNF-αis a mediator of the intestinal inflammatory processes,thus being one of the main cytokines involved in the pathogenesis of IBD,however,its levels,when measured,are present in the serum of patients with *** addition,TNF-αplays an important role in promoting inflammation,such as the production of interleukins(IL),for instance IL-1βand *** are two receptors for TNF as following:The tumor necrosis factor 1 receptor(TNFR1);and the tumor necrosis factor 2 receptor(TNFR2).They are involved in the pathogenesis of IBD and their receptors have been detected in IBD and their expression is correlated with disease *** soluble TNF form binds to the TNFR1 receptor with,and its activation results in a signaling cascade effects such as apoptosis,cell proliferation and cytokine *** contrast,the transmembrane TNF form can bind both to TNFR1 and *** studies have suggested that TNF-αis one of the main pro-inflammatory cytokines involved in the pathogenesis of IBD,since TNF levels are present in the serum of both patients with UC and *** and subcutaneous biologics targeting TNF-αhave revolutionized the treatment of IBD,thus becoming the best available agents to induce and maintain IBD *** application of antibodies aimed at neutralizing TNF-αin patients with IBD that induce a satisfactory clinical response in up to 60%of patients,and also induced long-term maintenance of disease remission in most *** has been suggested that anti-TNF-αagents inactivate the pro-inflammatory cytokine TNF-αby direct neutralization,i.e.,resulting in suppression of ***,anti-TNF-αantibodies perform more complex functions than a simple block

关键词： Tumor necrosis factor 1 receptor Tumor necrosis factor 2 receptor Inflammatory bowel diseases Enteric nervous system tumor necrosis factor-alpha

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Antioxidant Migration Studies in Chitosan Films Incorporated with Plant Extracts

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Journal of Renewable Materials 2018年第5期6卷 548-558页

作者： Victor Gomes Lauriano Souza patricia Freitas rodrigues Maria Paula Duarte Ana Luisa Fernando MEtRiCS Departamento de Ciências e Tecnologia da BiomassaFaculdade de Ciências e Tecnologia(FCT)Universidade NOVA de LisboaCampus da Caparica2829-516 CaparicaPortugal CENIMAT/I3N Departamento de Ciências dos MateriaisFaculdade de Ciências e Tecnologia(FCT)Universidade NOVA de LisboaCampus da Caparica2829-516 CaparicaPortugal

The aim of this work was to develop an active biopolymer based on chitosan by incorporating natural *** essential oils(ginger,rosemary,sage,tea tree and thyme)and six hydro-alcoholic extracts(from ginger,green and black tea,kenaf leaves,rosemary and sage plants)were *** assays were carried out to evaluate the films’activity,and total phenolic content and antioxidant activity were monitored in the simulant during *** between natural compounds and polymeric matrix was evaluated by FTIR *** diffusion of the phenolic compounds was not detected in the films incorporated with hydro-alcoholic extracts(HAEs),indicating their entrapment in the *** was observed in the films with essential oils(EOs),and biobased films incorporated with ginger,sage or rosemary essential oils,presented the highest diffusion and antioxidant activity in the simulant,highlighting their functionality and potential to be used as food active packaging material.

关键词： Active packaging antioxidant activity biopolymers chitosan phenolic content

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Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines

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Asian Pacific Journal of Tropical Biomedicine 2018年第7期8卷 345-351页

作者： Paulo Fernando Machado Paredes Selene Maia de Morais Fernando Cesar rodrigues Brito Luiz Francisco Wemmenson Goncalves Moura patricia de Araujo rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhaes Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes Northeast Biotechnology Network Graduate Program of Biotechnology State University of Ceara Itaperi campus department of Chemistry State University of Ceara Itaperi campus Laboratory of Bioprospecting of Natural Products and Biotechnology State University of CearaCECITEC

Objective: To evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: The high-performance liquid chromatography with diode-array detection and ~1H and ^(13)C nuclear magnetic resonance spectroscopy of the extract were used to distinguish the existence of possible functional groups in the root bark extract. The in vitro cytotoxic activity of methanol extract on human colon cancer cell lines was evaluated using OVCAR-8, SF-295, HCT-116, HL-60 strains and the samples were assessed by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide method. Results: The analysis of nuclear magnetic spectra of the active chloroform fraction revealed the presence of absorptions bands correspondent to a mixture of favelines such as neofavelanone, deoxofaveline or methyl-faveline, which structures were confirmed by ultraviolet spectra upon high-performance liquid chromatography with diode-array detection analysis. The active fraction showed cytotoxic effects in the tested strains, HCT-116, SF-295, OVCAR-8 and HL-60 cells with IC_(50) of 72 hours ranging from 4.95 to 15.23 μg/mL. Conclusions: The results suggest that the substances present in faveleira(Cnidoscolus quercifolius) root bark extract have a cytotoxic potential against several tumor lines, showing a broader antitumour potential, and in addition no adverse to healthy cells. Therefore, the root bark extract of Cnidoscolus quercifolius has a possibility of use for anticarcinogenic therapies.

关键词： Cnidoscolus quercifolius Pohl. methyl-faveline deoxofaveline Neofavelanone Faveline Cytotoxicity

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Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

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World Journal of Gastroenterology 2023年第22期29卷 3440-3468页

作者： Henrique Inhauser Riceti Magalhães Felipe Alexandre Machado Roberta Figueiroa Souza Marcos Antônio Ferreira caetano Vanessa Ribeiro Figliuolo Robson Coutinho-Silva patricia Castelucci department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil department of Anatomy University of São PauloSão Paulo 05508-000Brazil Biophysics Institute Carlos Chagas Filho Federal University of Rio de JaneiroRio de Janeiro 21941-902Brazil

BACKGROUND The literature indicates that the enteric nervous system is affected in inflammatory bowel diseases(IBDs)and that the P2X7 receptor triggers neuronal ***,the mechanism by which enteric neurons are lost in IBDs is *** To study the role of the caspase-3 and nuclear factor kappa B(NF-κB)pathways in myenteric neurons in a P2X7 receptor knockout(KO)mouse model of *** Forty male wild-type(WT)C57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid(colitis group).Mice in the sham groups were injected with *** mice were divided into eight groups(n=5):The WT sham 24 h and 4 d groups,the WT colitis 24 h and 4 d groups,the KO sham 24 h and 4 d groups,and the KO colitis 24 h and 4 d *** disease activity index(DAI)was analyzed,the distal colon was collected for immunohistochemistry analyses,and immunofluorescence was performed to identify neurons immunoreactive(ir)for calretinin,P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,and total NF-κ*** analyzed the number of calretinin-ir and P2X7 receptor-ir neurons per ganglion,the neuronal profile area(μm^(2)),and corrected total cell fluorescence(CTCF).RESULTS Cells double labeled for calretinin and P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,or total NF-κB were observed in the WT colitis 24 h and 4 d *** number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups,respectively(2.10±0.13 vs 3.33±0.17,Pdecreased in the WT colitis 4 d group compared to the WT sham 4 d group(104.63±2.49 vs 117.41±1.14,P<0.01).The number of

关键词： Cell death Enteric nervous system Gastroenterology Inflammatory bowel diseases P2X7 receptor Purinergic signaling

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Risk factors for suicidal behaviour in late-life depression:A systematic review

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World Journal of Psychiatry 2022年第1期12卷 187-203页

作者： Veronica Fernandez-rodrigues Yolanda Sanchez-Carro Luisa Natalia Lagunas Laura Alejandra Rico-Uribe Andres Pemau patricia Diaz-Carracedo Marina Diaz-Marsa Gonzalo Hervas Alejandro de la Torre-Luque department of Psychology Universidad Complutense de MadridMadrid 28223Spain department of Psychiatry Universidad Autonoma de MadridMadrid 28046Spain Centre for Biomedical Research in Mental Health(CIBERSAM) Madrid 28029Spain department of Legal Medicine Psychiatry and PathologyUniversidad Complutense de MadridMadrid 28046Spain of Psychology La Rioja International UniversityLogrono 26006Spain Institute of Psychiatry and Mental Health San Carlos Clinical HospitalMadrid 28040Spain

BACKGROUND Suicide is a leading cause of preventable death worldwide,with its peak of maximum incidence in later *** often puts an individual at higher risk for suicidal *** turn,depression deserves particular interest in old age due to its high prevalence and dramatic impact on health and *** To gather integrated evidence on the potential risk factors for suicide behaviour development in depressive older adults,and to examine the effects of depression treatment to tackle suicide behaviour in this *** A systematic review of empirical studies,published from 2000 onwards,was *** behaviour was addressed considering its varying forms(i.e.,wish to die,ideation,attempt,and completed suicide).RESULTS Thirty-five papers were selected for review,comprising both clinical and epidemiological *** of studies focused on suicidal ideation(60%).The studies consistently pointed out that the risk was related to depressive episode severity,psychiatric comorbidity(anxiety or substance use disorders),poorer health status,and loss of *** social support and loneliness were also associated with suicide behaviour in depressive older ***,the intervention studies showed that suicidal behaviour was a robust predictor of depression treatment *** in suicidal ideation were moderated by reductions in risk factors for suicide *** To sum up,common and age-specific risk factors seem to be involved in suicide development in depressive older adults.A major effort should be made to tackle this serious public health concern so as to promote older people to age healthily and well.

关键词： Late-life depression Suicide behaviour Disability Chronic disease Loneliness

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Serological Evidence of Human Coinfection by Brazilian Spotted Fever and Bartonellosis

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International Journal of Clinical Medicine 2016年第11期7卷 766-770页

作者： Otília Lupi Eula Carvalho Tatiana Rozental Alexsandra rodrigues de Mendonça Favacho Elba Regina Sampaio de Lemos patricia Brasil Laboratório de Doenças Febris Agudas Instituto Nacional de Infectologia Evandro Chagas (INI) Funda&ccedil&atildeo Oswaldo Cruz (FIOCRUZ) Rio de Janeiro Brazil Laboratório de Hantaviroses e Rickettsioses Instituto Oswaldo Cruz (IOC) Funda&ccedil&atildeo Oswaldo Cruz (FIOCRUZ) Rio de Janeiro Brazil

Brazilian spotted fever and bartonellosis are zoonotic, emerging and under diagnosed diseases. Pets may be co-infected by multiple pathogens and become transmissions sources to humans. The study reports the first case of active co-infection by Brazilian spotted fever and bartonellosis based on serological evidence. The authors aim to demonstrate the importance of performing systematic syndromic investigations on nonspecific febrile syndromes, guided by the epidemiological history and considering the possibility of co-infection by zoonosis sharing the same ecological niche.

关键词： Rickettsia rickettsia Bartonella henselae Brazilian Spotted Fever Bartonellosis Co-Infection

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