Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells

作     者：Zhule Wang Liebert Parreiras Nogueira Håvard Jostein Haugen Ingrid CM.Van Der Geest Patricia Caetano de Almeida Rodrigues Dennis Janssen Thom Bitter Jeroen J.J.P.van den Beucken Sander CG.Leeuwenburgh 

作者机构：Radboud University Medical CenterDepartment of Dentistry-Regenerative BiomaterialsRadboud Institute for Molecular Life SciencesNijmegenthe Netherlands University of OsloDepartment of BiomaterialsInstitute of Clinical DentistryFaculty of DentistryOsloNorway Radboud University Medical CenterDepartment of OrthopedicsRadboud Institute for Health SciencesNijmegenthe Netherlands 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第9期

页      面：120-130页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 100214[医学-肿瘤学] 0836[工学-生物工程] 10[医学] 

基　　金：funded by China Scholarship Council 

主　　题：Porous polymethylmethacrylate cement Bone tumor treatment Local drug delivery Chemotherapeutic drug Ex vivo biomechanical assessment 

摘      要：Malignant bone tumors are usually treated by resection of tumor tissue followed by filling of the bone defect with bone graft ***(PMMA)cement is the most commonly used bone substitute in clinical orthopedics in view of its ***,the dense nature of PMMA renders this biomaterial unsuitable for local delivery of chemotherapeutic drugs to limit the recurrence of bone ***,we introduce porosity into PMMA cement by adding carboxymethylcellulose(CMC)to facilitate such local delivery of chemotherapeutic drugs,while retaining sufficient mechanical properties for bone reconstruction in load-bearing *** results show that the mechanical strength of PMMA-based cements gradually decreases with increasing CMC *** incorporation of≥3%CMC,the PMMA-based cements released up to 18%of the loaded cisplatin,in contrast to cements containing lower amounts of CMC which only released less than 2%of the cisplatin over 28 *** release of cisplatin efficiently killed osteosarcoma cells in vitro and the fraction of dead cells increased to 91.3%at day 7,which confirms the retained chemotherapeutic activity of released cisplatin from these PMMA-based ***,tibias filled with PMMA-based cements containing up to 3%of CMC exhibit comparable compressive strengths as compared to intact *** conclusion,we demonstrate that PMMA cements can be rendered therapeutically active by introducing porosity using CMC to allow for release of cisplatin without compromising mechanical properties beyond critical *** such,these data suggest that our dual-functional PMMA-based cements represent a viable treatment option for filling bone defects after bone tumor resection in load-bearing sites.