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有一未配对残基T的DnA [d(TCGCGCG)]_2结构特征的nMR研究

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中国科学（B辑） 1994年第1期24卷 23-28页

作者：张友杰王可玉 p.n.borer 华中师范大学分析测试中心武昌430070 美国Syracuse大学核磁共振与数据处理实验室 syracuseny13244

具有交替的嘧啶-嘌呤序列和5′端有一未配对残基T的DnA七聚体[d(TCGCGCG)]_2可以作为研究DnA双螺旋的双链-单链联结构象特征的模本。DnA的这种联结方式存在于它们的复制和转录过程中。用nOESY和TOCSY/HOHAHA实验对其共振峰作了指定。对... 详细信息

具有交替的嘧啶-嘌呤序列和5′端有一未配对残基T的DnA七聚体[d(TCGCGCG)]_2可以作为研究DnA双螺旋的双链-单链联结构象特征的模本。DnA的这种联结方式存在于它们的复制和转录过程中。用nOESY和TOCSY/HOHAHA实验对其共振峰作了指定。对七个不同混合时间的nOE强度进行了定量分析,用双自旋近似法计算了质子间的距离,揭示了这个分子中令人感兴趣的结构特征。用***实验测定了该DnA双螺旋各糖质子间偶合常数,鉴定了各糖的构象。从而获得了该DnA七聚体在溶液中的结构特征。就整体结构而言,该DnA分子仍属B族DnA,但结构有了很大变化,特别是在端基区。未配对的残基具有不同寻常的顺式构象。为DnA双链-单链联结部分构象提供了有用的借鉴。

关键词： DnA 构象 nMR 七聚核甙酸

DnA的^(13)C化学位移与其链间嘌呤-嘌呤碰撞的相关性

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中国科学（B辑） 1991年第12期 1259-1266页

作者：张友杰王可玉 p.n.borer G.C.Levy 华中师范大学分析测试中心武昌430070 美国Syracuse大学核磁共振与数据处理实验室 syracuseny13244

本文发现并间接证实了DnA的^(13)C化学位移与其结构参数(主轴螺旋扭曲角Ω,碱基平面滚动角ρ,主链扭曲角δ和螺旋浆式扭曲角ω)之间存在着相关性,文中阐明了与这些结构参数相关的碳及其理论上的解释。这些碳的化学位移相对变化明显地反... 详细信息

本文发现并间接证实了DnA的^(13)C化学位移与其结构参数(主轴螺旋扭曲角Ω,碱基平面滚动角ρ,主链扭曲角δ和螺旋浆式扭曲角ω)之间存在着相关性,文中阐明了与这些结构参数相关的碳及其理论上的解释。这些碳的化学位移相对变化明显地反映出DnA链间嘌呤-嘌呤碰撞(立体障碍)所引起的DnA双螺旋局部结构的变化,并与Calladine-Dickerson加和函数Σ作了比较。从DnA的^(13)C nMR谱可以得到有关其构象的信息,从而有可能通过^(13)C谱观测DnA在溶液中的Ω,ρ,δ和ω等结构参数。

关键词：脱氧核糖核酸 nMR 嘌呤碰撞

DnA双螺旋的13C化学位移与其结构参数的相关性

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DNA双螺旋的13C化学位移与其结构参数的相关性

有一摆动残基T的DnA[d(TCGCGCG)]2结构特征的nMR研究

第八届全国波谱学学术会议

作者：张友杰王可玉 p.n.borer 华中师范大学分析测试中心 nMR and Data processing Laboratory Syracuse UniversitySyracuseNY 13244-4100USA

本文以一低聚DnA双螺旋[d(CGTACGTACG)]的化学位移相对变化进一步证实了作者们曾经发现的DnA的C化学位移与其结构参数之间存在的相关性,并与Calladine加和函数∑—∑作了比较,即C与主轴螺旋扭曲角相关(图1);C(嘧啶)、C(嘌呤)与碱基平

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有一摆动残基T的DNA[d(TCGCGCG)]2结构特征的NMR研究

Impacts of pI3K/protein kinase B pathway activation in reactive astrocytes: from detrimental effects to protective functions

第七届全国波谱学学术会议

作者：王可玉张友杰 p.n.borer G.C.Levy 美国Syracuse大学nMR和数据处理实验室华中师范大学分析测试中心

具有交替的嘧啶-嘌呤序列和在5′端有一摆动残基T的DnA片断[d (TCGCGCG)]可以用于研究双链一单链联结的DnA双螺旋构象特征的模本.这种联结方式存在于DnA的复制和转录过程之中。我们对该分子进行了1D、2D nOE

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来源： cnki会议评论

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neural Regeneration Research 2025年第4期20卷 1031-1041页

作者： Ramón pérez-núñez María Fernanda González Ana María Avalos Lisette Leyton Cellular Communication Laboratory Programa de Biología Celular y MolecularCenter for Studies on ExerciseMetabolism and Cancer(CEMC)Instituto de Ciencias Biomédicas(ICBM)Facultad de MedicinaUniversidad de ChileSantiagoChile Advanced Center for Chronic Diseases(ACCDiS) Faculty of Chemical and Pharmaceutical Sciences&Faculty of MedicineUniversidad de ChileSantiagoChile Instituto de Ciencias Biomédicas Facultad de Ciencias de la SaludUniversidad Autónoma de ChileSantiagoChile

Astrocytes are the most abundant type of glial cell in the central nervous *** injury and inflammation,astrocytes become reactive and undergo morphological and functional *** on their phenotypic classification as A1 or A2,reactive astrocytes contribute to both neurotoxic and neuroprotective responses,***,this binary classification does not fully capture the diversity of astrocyte responses observed across different diseases and *** analysis has revealed that reactive astrocytes have a complex landscape of gene expression profiles,which emphasizes the heterogeneous nature of their *** actively participate in regulating central nervous system inflammation by interacting with microglia and other cell types,releasing cytokines,and influencing the immune *** phosphoinositide 3-kinase(pI3K)/protein kinase B(AKT)signaling pathway is a central player in astrocyte reactivity and impacts various aspects of astrocyte behavior,as evidenced by in silico,in vitro,and in vivo *** astrocytes,inflammatory cues trigger a cascade of molecular events,where nuclear factor-κB serves as a central mediator of the pro-inflammatory ***,we review the heterogeneity of reactive astrocytes and the molecular mechanisms underlying their *** highlight the involvement of various signaling pathways that regulate astrocyte reactivity,including the pI3K/AKT/mammalian target of rapamycin(mTOR),αvβ3 integrin/pI3K/AKT/connexin 43,and notch/pI3K/AKT *** targeting the inactivation of the pI3K/AKT cellular signaling pathway to control reactive astrocytes and prevent central nervous system damage,evidence suggests that activating this pathway could also yield beneficial *** dual function of the pI3K/AKT pathway underscores its complexity in astrocyte reactivity and brain function *** review emphasizes the importance of employing astrocyte-exclusive models to underst

关键词： inflammation integrins neuroprotective neurotoxic phosphatidylinositol 3-kinase reactive astrocytes signal transduction Thy-1(CD90)

From single to combinatorial therapies in spinal cord injuries for structural and functional restoration

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neural Regeneration Research 2025年第3期20卷 660-670页

作者： Ernesto Doncel-pérez Gabriel Guízar-Sahagún Israel Grijalva-Otero Hospital nacional de parapléjicos de Toledo Servicio de Salud de Castilla La Mancha(SESCAM)ToledoSpain Medical Research Unit for neurological Diseases UMAE Hospital de EspecialidadesCentro Médico Nacional Siglo XXIIMSSCiudad de MéxicoMéxico

Spinal cord injury results in paralysis, sensory disturbances, sphincter dysfunction, and multiple systemic secondary conditions, most arising from autonomic dysregulation. All this produces profound negative psychosocial implications for affected people, their families, and their communities;the financial costs can be challenging for their families and health institutions. Treatments aimed at restoring the spinal cord after spinal cord injury, which have been tested in animal models or clinical trials, generally seek to counteract one or more of the secondary mechanisms of injury to limit the extent of the initial damage. Most published works on structural/functional restoration in acute and chronic spinal cord injury stages use a single type of treatment: a drug or trophic factor, transplant of a cell type, and implantation of a biomaterial. Despite the significant benefits reported in animal models, when translating these successful therapeutic strategies to humans, the result in clinical trials has been considered of little relevance because the improvement, when present, is usually insufficient. Until now, most studies designed to promote neuroprotection or regeneration at different stages after spinal cord injury have used single treatments. Considering the occurrence of various secondary mechanisms of injury in the acute and sub-acute phases of spinal cord injury, it is reasonable to speculate that more than one therapeutic agent could be required to promote structural and functional restoration of the damaged spinal cord. Treatments that combine several therapeutic agents, targeting different mechanisms of injury, which, when used as a single therapy, have shown some benefits, allow us to assume that they will have synergistic beneficial effects. Thus, this narrative review article aims to summarize current trends in the use of strategies that combine therapeutic agents administered simultaneously or sequentially, seeking structural and functional restoratio

关键词： neural regeneration neuroprotection spinal cord injury repair spinal cord injury treatments structural restoration of spinal cord injury

nucleoside modified mRnA-lipid nanoparticles as a new delivery platform for the repair of the injured spinal cord

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neural Regeneration Research 2025年第8期20卷 2311-2312页

作者： Krisztián pajer Tamás Bellák Antal nógrádi Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary

Spinal cord injury and treatment opportunities:The adult mammalian spinal cord has a very limited capacity for spontaneous regeneration due to various intrinsic molecular and cellular *** the spinal cord neurons have the capacity to regenerate their axons,the expression of growth inhibitory factors,lack or suppression of proper guidance cues,and profound inflammatory responses do not permit successful regeneration(Khyeam et al.,2021).

关键词： injured capacity modified

Sex differences in aging and injured brain

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neural Regeneration Research 2025年第10期20卷 2901-2902页

作者： Jordan n.Williamson Yuan Yang University of Illinois Urbana-Champaign Grainger College of EngineeringDepartment of BioengineeringUrbanaILUSA Carle Foundation Hospital Stephenson Family Clinical Research InstituteClinical Imaging Research CenterUrbanaILUSA University of Illinois Urbana-Champaign Beckman Institute for Advanced Science and TechnologyUrbanaILUSA northwestern University Department of Physical Therapy and Human Movement SciencesChicagoILUSA

Background:The prevalence,age of onset,and symptomatology of traumatic brain injury,stroke,and neurodegenerative diseases(such as Alzheimer’s disease(AD),parkinson’s disease,amyotrophic lateral sclerosis,and Huntington’s disease)differ substantially between males and *** higher prevalence of these brain disorders has been attributed to females having a greater longevity compared with *** one of the greatest risk factors of acquired brain injury(such as stroke,traumatic brain injury caused by fall)and neurodegenerative disease is age.

关键词： Huntington prevalence traumatic

Astrocytes, reactive astrogliosis, and glial scar formation in traumatic brain injury

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neural Regeneration Research 2025年第4期20卷 973-989页

作者： María Belén Cieri Alberto Javier Ramos Laboratorio de neuropatología Molecular IBCN UBA-CONICETFacultad de MedicinaUniversidad de Buenos AiresBuenos AiresArgentina

Traumatic brain injury is a global health crisis,causing significant death and disability *** that follows traumatic brain injury has serious consequences for neuronal survival and cognitive impairments,with astrocytes involved in this *** traumatic brain injury,astrocytes rapidly become reactive,and astrogliosis propagates from the injury core to distant brain *** astroglial proteins are downregulated near the traumatic brain injury core,while pro-inflammatory astroglial genes are *** altered gene expression is considered a pathological remodeling of astrocytes that produces serious consequences for neuronal survival and cognitive *** addition,glial scar formed by reactive astrocytes is initially necessary to limit immune cell infiltration,but in the long term impedes axonal reconnection and functional *** therapeutic strategies for traumatic brain injury are focused on preventing acute ***,cannabinoids,progesterone,beta-blockers,and cerebrolysin demonstrate neuroprotective benefits but most of them have not been studied in the context of *** this review,we discuss the cell signaling pathways activated in reactive astrocytes following traumatic brain injury and we discuss some of the potential new strategies aimed to modulate astroglial responses in traumatic brain injury,especially using cell-targeted strategies with miRnAs or lncRnA,viral vectors,and repurposed drugs.

关键词： astrocyte glial scar innate immunity neuroinflammation stab injury Toll-like receptors