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Carbon Resources Conversion 2021年第1期4卷 61-75页

作者： Sofia Sarantou Nikolaos G.Stoforos ourania kalantzi Seraphim Papanikolaou Department of Food Science&Human Nutrition Agricultural University of Athens75 Iera Odos11855 AthensGreece

Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole *** strains *** DSM 4444 and *** ACA-DC 5033 displayed better performances and were further *** initial glycerol(Gly0)≈50 g/L,the strain ACA-DC 5033 produced dry cell weight(DCW)≈7.0 g/L,and also secondary extra-cellular metabolites as citric acid(CA)at≈16 g/L and polyols(Pol)(mannitol,erythritol and arabitol)at≈21 g/*** double-media(besides nitrogen also Mg was limiting)for Gly0≈50 g/L,a shift towards CA production occurred(CAmax≈33 g/L).The strain DSM 4444 at Gly0≈50 g/L produced DCW=18.1 g/L containing lipids=30.3%in *** single nitrogen-limited media with Gly0≈90 g/L,the strain ACA-DC 5033 produced Pol≈48 g/L and CA≈20 g/L,while the strain DSM 4444 produced DCW=27.3 g/L containing lipid=54.5%w/*** the late growth phases,metabolites were *** growth phase(trophophase)and phase of secondary metabolite synthesis(idiophase)were successfully simulated with the aid of a modified Velhlust-Aggelis *** extraction process was studied for these two strains by using two different extraction *** lipids contained mostly oleic acid,constituting suitable precursors for the synthesis of 2nd generation biodiesel.

关键词： Biodiesel-derived glycerol Microbial lipid Polyols Rhodosporidium toruloides Yarrowia lipolytica

Effect of growth hormone on colonic anastomosis after intraperitoneal administration of 5-fluorouracil, bleomycin and cisplatin: An experimental study

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2679-2688页

作者： Ioannis Lambrou Ioannis Mantzoros Orestis Ioannidis Dimitrios Tatsis Elissavet Anestiadou Vasiliki Bisbinas Manousos-Georgios Pramateftakis Efstathios Kotidis Barbara Driagka ourania Kerasidou Savvas Symeonidis Stefanos Bitsianis Freideriki Sifaki Konstantinos Angelopoulos Haralabos Demetriades Stamatios Angelopoulos Fourth Department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece Department of ENT Royal Cornwall Hospitals NHS TrustCornwall TR13LJUnited Kingdom

BACKGROUND Growth hormone(GH)plays a crucial role in wound healing and tissue repair in postoperative *** particular,colonic anastomosis healing following colorectal surgery is impaired by numerous chemotherapy *** To investigate whether GH can improve the healing of a colonic anastomosis following the adverse effects of intraperitoneal administration of 5-fluorouracil(5-FU),bleomycin and *** Eighty Wistar rats underwent laparotomy and a 1 cm-resection of the transverse colon,followed by an end-to-end anastomosis under general *** rats were blindly allocated into four equal groups and administered a different daily intraperitoneal therapeutic regimen for 6 *** control group(A)received normal *** B received chemotherapy with 5-FU(20 mg/kg),bleomycin(4 mg/kg)and cisplatin(0.7 mg/kg).Group C received GH(2 mg/kg),and group D received the aforementioned combination chemotherapy and GH,as *** rats were sacrificed on the 7th postoperative day and the anastomoses were macroscopically and microscop-ically *** weight,bursting pressure,hydroxyproline levels and inflammation markers were *** All rats survived until the day of sacrifice,with no infections or other complications.A decrease in the body weight of group D rats was observed,not statistically significant compared to group A(P=1),but significantly different to groups C(P=0.001)and B(P<0.01).Anastomotic dehiscence rate was not statistically different between the *** pressure was not significantly different between groups A and D(P=1.0),whereas group B had a significantly lower bursting pressure compared to group D(P<0.001).All groups had significantly more adhesions than group ***,as a measurement of collagen deposition,was significantly higher in group D compared to group B(P<0.05),and higher,but not statistically significant,compared to group *** changes in group D were recorded,compared to group A

关键词： Growth hormone Colonic anastomosis Adhesion Bursting pressure Collagen Hydroxyproline Inflammation Neoangiogenesis Chemotherapy

Epigenetics in hepatocellular carcinoma: An update and future therapy perspectives

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World Journal of Gastroenterology 2014年第2期20卷 333-345页

作者： Li Ma Mei-Sze Chua ourania Andrisani Samuel So Asian Liver Center and Department of Surgery Stanford University School of MedicineStanford UniversityStanfordCA 94305United States Department of Basic Medical Science and Center for Cancer Research Purdue UniversityWest LafayetteIN 47907United States

Hepatocellular carcinoma(HCC),the predominant form of adult liver malignancies,is a global health *** dismal prognosis has prompted recent significant advances in the understanding of its etiology and *** deregulation of epigenetic mechanisms,which maintain heritable gene expression changes and chromatin organization,is implicated in the development of multiple cancers,including *** review summarizes the current knowledge of epigenetic mechanisms in the pathogenesis of HCC,with an emphasis on HCC mediated by chronic hepatitis B virus *** review also discusses the encouraging outcomes and lessons learnt from epigenetic therapies for hematological and other solid cancers,and highlights the future potential of similar therapies in the treatment of HCC.

关键词： Hepatocellular carcinoma Epigenetics DNA methylation Histone modification miRNA lncRNA

Increased ΤGF-β3 in primary biliary cirrhosis: An abnormality related to pathogenesis?

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World Journal of Gastroenterology 2010年第40期16卷 5057-5064页

作者： Argyro Voumvouraki Mairi Koulentaki Maria Tzardi ourania Sfakianaki Penelope Manousou George Notas Elias Kouroumalis University Hospital Department of Gastroenterology University of Crete Faculty of Medicine Liver Research Laboratory University of Crete Faculty of Medicine University Hospital Department of Pathology University of Crete Faculty of Medicine Laboratory of Experimental Endocrinology University of Crete Faculty of Medicine

AIM: To investigate the transforming growth factor-β (TGF-β) isoforms in the peripheral and hepatic venous blood of primary biliary cirrhosis (PBC) patients. METHODS: We examined TGF-β1, TGF-β2 and TGF-β3 (enzyme-linked immunosorbent assay), in 27 stage Ⅳ PBC patients (27 peripheral and 15 hepatic vein sera), 35 early (Ⅰ-Ⅱ) PBC and 60 healthy controls. As disease controls 28 hepatitis C virus (HCV) cirrhosis (28 peripheral and 17 hepatic vein serum), 44 chronic HCV hepatitis and 38 HCV-related hepatocellular carcinomas were included. We also tested liver tissue by immunohistochemistry to identify localization of TGF isoforms. RESULTS: TGF-β1 was significantly decreased in all cirrhotics (PBC Ⅲ-Ⅳ: median 13.4 ng/mL; range, 7.4-26.2, HCV cirrhosis: 11.6 ng/mL; range, 5.0-33.8), compared to controls (30.9 ng/mL; range, 20.9-37.8). TGF-β2 was increased in viral cirrhosis but not in PBC and chronic hepatitis. TGF-β3 (47.2 pg/mL; range, 27.0-79.7 in healthy controls) was increased in early and late PBC (Ⅰ-Ⅱ: 94.3 pg/mL; range, 41.5-358.6; Ⅲ-Ⅳ: 152.8 pg/mL; range, 60.4-361.2; P < 0.001) and decreased in viral cirrhosis (37.4 pg/mL; range, 13.3-84.0; P < 0.05). Hepatic vein TGF-β levels were analogous to those in peripheral blood. Immunohistochemistry identified all isoforms in portal tract lymphocytes, sinusoidal cells and cholangiocytes. TGF-β3 was additionally overexpressed in hepatocytes in PBC patients. CONCLUSION: The serum profile of TGF-β isoforms is different in cirrhotics. Increased TGF-β3 is characteristic of PBC. These findings may be related to the immunological abnormalities of PBC.

关键词： Transforming growth factor-β Primary biliary cirrhosis Liver fibrosis Cirrhosis

Peri-nuclear antibodies correlate with survival in Greek primary biliary cirrhosis patients

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World Journal of Gastroenterology 2010年第39期16卷 4938-4943页

作者： ourania Sfakianaki Meri Koulentaki Maria Tzardi Elena Tsangaridou Panayotis A Theodoropoulos Elias Castanas Elias A Kouroumalis Liver Research Laboratory Medical SchoolUniversity of Crete Department of Gastroenterology University Hospital of Heraklion Department of Pathology University Hospital of Heraklion Department of Biochemistry Medical SchoolUniversity of Crete Department of Experimental Endocrinology Medical SchoolUniversity of Crete Liver ResearchLaboratory Medical SchoolUniversity of Crete

AIM:To investigate possible associations of anti-nuclear envelope antibody(ANEA)with disease severity and survival in Greek primary biliary cirrhosis(PBC)***:Serum samples were collected at diagnosis from 147 PBC patients(85%female),who were followed-up for a median 89.5 mo(range 1-240).ANEA were detected with indirect immunofluorescence on 1% formaldehyde fixed Hep2 cells,and anti-gp210 antibodies were detected using an enzyme linked immunosorbent *** were correlated with clinical data,histology,and ***:ANEA were detected in 69/147(46.9%) patients and 31/147(21%)were also anti-gp210 *** ANEA positive patients were at a more advanced histological stage(Ⅰ-Ⅱ/Ⅲ-Ⅳ56.5%/43.5% vs 74.4%/25.6%,P=0.005)compared to the ANEA negative *** had a higher antimitochondrial antibodies(AMA)titer(≤1:160/>1:160 50.7%/49.3%vs 71.8%/28.2%,P=0.001)and a lower survival time(91.7 ±50.7 mo vs 101.8±55 mo,P=0.043).Moreover,they had more advanced fibrosis,portal inflammation,interface hepatitis,and proliferation of bile ductules(P =0.008,P=0.008,P=0.019,and P=0.027,respectively).They also died more frequently of hepatic failure and/or hepatocellular carcinoma(P=0.016).ANEA positive,anti-gp210 positive patients had a difference in stage(Ⅰ-Ⅱ/Ⅲ-Ⅳ54.8%/45.2%vs 74.4%/25.6%,P= 0.006),AMA titer(≤1:160/>1:160 51.6%/48.4%vs 71.8%/28.2%,P=0.009),survival(91.1±52.9 mo vs 101.8±55 mo,P=0.009),and Mayo risk score(5.5 ±1.9 vs 5.04±1.3,P=0.04)compared to the ANEA negative *** positive,anti-gp210 negative patients had a difference in AMA titer(≤1:160/>1:160 50%/50%vs 71.8%/28.2%,P=0.002),stage(Ⅰ-Ⅱ/Ⅲ -Ⅳ57.9%/42.1%vs 74.4%/25.6%,P=0.033),fibrosis(P=0.009),portal inflammation(P=0.018),interface hepatitis(P=0.032),and proliferation of bile ductules(P=0.031).Anti-gp210 positive patients had a worse Mayo risk score(5.5±1.9 vs 4.9±1.7,P=0.038)than the anti-gp210 negative ***:The presence of ANEA and anti-gp210 identifies a subgroup of PBC pati

关键词： Primary biliary cirrhosis Antimitochondrial antibodies Antinuclear antibodies Antibodies against nuclear envelope antigens Anti-gp210 antibodies

LIX1-like protein drives hepatic stellate cell activation to promote liver fibrosis by regulation of chemokine mRNA stability

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Signal Transduction and Targeted Therapy 2021年第10期6卷 2950-2953页

作者： Xiaoyun Zhu Yanqiu Zhang Yucheng Zhao Dejuan Xiang Jie Zou ourania Andrisani Hao Zhang Lingyi Kong Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjingChina Department of Basic Medical Sciences and Purdue Center for Cancer Research Purdue UniversityWest LafayetteINUSA

Dear Editor,Hepatic stellate cells(HSCs)play a key role in the fibrotic response,thus inactivating activated HSC could be a potential therapy for fibrosis.^(1,2) CCL20 expressed by HSCs and macrophages,may serve as a mediator of in flammation and fibrosis.^(3) LIX1L is a putative RNA-binding protein(RBP)that may play an important role in post-transcriptional gene regulation.^(4) However,the biological function of LIX1L in liver fibrosis remains unclear,we therefore aimed to characterize its functions in HSC activation and liver fibrosis.

关键词： CCL20 liver activation

Effectiveness of high intensity interval training on cardiorespiratory fitness and endothelial function in type 2 diabetes:A systematic review

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World Journal of Cardiology 2023年第4期15卷 184-199页

作者： Christos Kourek Eleftherios Karatzanos Vasiliki Raidou ourania Papazachou Anastassios Philippou Serafim Nanas Stavros Dimopoulos Clinical Ergospirometry Exercise and Rehabilitation Laboratory1st Critical Care Medicine DepartmentEvangelismos HospitalAthens 10676Greece Department of Cardiology 417 Army Share Fund Hospital of AthensAthens 11521Greece Clinical Ergospirometry Exercise and Rehabilitation LaboratoryNational and Kapodistrian University of AthensAthens 10676Greece Department of Cardiology "Helena Venizelou"HospitalAthens 10676Greece Department of Physiology School of MedicineNational and Kapodistrian University of AthensAthens 11527Greece Cardiac Surgery Intensive Care Unit Onassis Cardiac Surgery CenterAthens 17674Greece

BACKGROUND Type 2 diabetes mellitus(T2DM)is a chronic metabolic syndrome characterized by insulin resistance and hyperglycemia that may lead to endothelial dysfunction,reduced functional capacity and exercise *** aerobic exercise has been promoted as the most beneficial non-pharmacological treatment of cardiovascular *** intensity interval training(HIIT)seems to be superior than moderate-intensity continuous training(MICT)in cardiovascular diseases by improving brachial artery flow-mediated dilation(FMD)and cardiorespiratory fitness to a greater ***,the beneficial effects of HIIT in patients with T2DM still remain under investigation and number of studies is *** To evaluate the effectiveness of high intensity interval training on cardiorespiratory fitness and endothelial function in patients with *** We performed a search on PubMed,PEDro and CINAHL databases,selecting papers published between December 2012 and December 2022 and identified published randomized controlled trials(RCTs)in the English language that included community or outpatient exercise training programs in patients with *** were assessed for methodological rigor and risk of bias via the Physiotherapy Evidence Database(PEDro).The primary outcome was peak VO_(2 ) and the secondary outcome was endothelial function assessed either by FMD or other indices of *** Twelve studies were included in our systematic *** 12 RCTs resulted in 661 participants in *** was performed in 310 patients(46.8%),MICT to 271 and the rest 80 belonged to the control *** VO_(2 ) increased in 10 out of 12 studies after *** studies compared HIIT with other exercise regimens(MICT or strength endurance)and 4 of them demonstrated additional beneficial effects of HIIT over MICT or other exercise ***,4 studies explored the effects of HIIT on endothelial function and FMD in T2DM *** 2 of them,HIIT further impro

关键词： Type 2 diabetes mellitus Exercise High intensity interval training Cardiorespiratory fitness Peak VO_(2) Endothelial function

Effects of exercise training on diastolic and systolic dysfunction in patients with chronic heart failure

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World Journal of Cardiology 2021年第9期13卷 514-525页

作者： Ioannis Chaveles ourania Papazachou Manal al Shamari Dimitrios Delis Argirios Ntalianis Niki Panagopoulou Serafim Nanas Eleftherios Karatzanos 1st Department of Cardiology-Clinical Ergospirometry Exercise and Rehabilitation Laboratory“Evaggelismos”HospitalAthens 10676Greece Department of Cardiology ”Helena Venizelou”HospitalAthens 10676Greece Clinical Ergospirometry Exercise and Rehabilitation Laboratory”Evaggelismos”HospitalSchool of MedicineNational and Kapodistrian University of AthensAthens 10676Greece Heart Failure Unit Department of Clinical Therapeutics”Alexandra”HospitalNational and Kapodistrian University of AthensAthens 11528Greece

BACKGROUND Chronic heart failure(CHF)is a complex syndrome characterized by a progressive reduction of the left ventricular(LV)contractility,low exercise tolerance,and increased mortality and *** dysfunction(DD)of the LV,is a keystone in the pathophysiology of CHF and plays a major role in the progression of most cardiac ***,it is well estimated that exercise training induces several beneficial effects on patients with *** To evaluate the impact of a cardiac rehabilitation program on the DD and LV ejection fraction(EF)in patients with *** Thirty-two stable patients with CHF(age:56±10 years,EF:32%±8%,88%men)participated in an exercise rehabilitation *** were randomly assigned to aerobic exercise(AER)or combined aerobic and strength training(COM),based on age and peak oxygen uptake,as stratified randomization *** and after the program,they underwent a symptom-limited maximal cardiopulmonary exercise testing(CPET)and serial echocardiography evaluation to evaluate peak oxygen uptake(VO2peak),peak workload(Wpeak),DD grade,right ventricular systolic pressure(RVSP),and *** The whole cohort improved VO2peak,and Wpeak,as well as DD grade(P0.05).CONCLUSION Exercise rehabilitation improves indices of diastolic and systolic *** protocol was not observed to affect *** results need to be further investigated in larger samples.

关键词： Chronic heart failure Cardiovascular effects Cardiac rehabilitation Aerobic exercise Strength training Diastolic dysfunction

Antithrombotic therapy in TAVI

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Journal of Geriatric Cardiology 2018年第1期15卷 66-75页

作者： Manolis Vavuranakis Konstantinos Kalogeras Angelos Michail Kolokathis Dimitrios Vrachatis Nikolaos Magkoutis Gerasimos Siasos Euaggelos Oikonomou Maria Kariori Theodoros Papaioannou Maria Lavda Carmen Moldovan ourania Katsarou Dimitrios Tousoulis The First Department of Cardiology Hippokration Hospital Medical School National & Kapodistrian University of Athens Ilioupoli Greece

Transcatheter aortic valve implantation （TAVI） carries a significant thromboembolic and concomitant bleeding risk, not only during the procedure but also during the periprocedural period. Many issues concerning optimal antithrombotic therapy after TAVI are still under debate. In the present review, we aimed to identify all relevant studies evaluating antithrombotic therapeutic strategies in relation to clinical outcomes after the procedure. Four randomized control trials （RCT） were identified analyzing the post-TAVI antithrombotic strategy with all of them utilizing aspirin lifelong plus clopidogrel for 3-6 months. Seventeen registries have been identified, with a wide variance among them regarding baseline characteristics, while concerning antiplatelet therapy, clopidogrel duration was ranging from 3-12 months. Four non-randomized trials were identified, comparing single vs. dual antiplatelet therapy after TAVI, in respect of investigating thromboembolic outcome events over bleeding complications. Finally, limited data from a single RCT and a retrospective study exist with regards to anticoagulant treatment during the procedure and the optimal antithrombotic therapy when concomitant atrial fibrillation. In conclusion, due to the high risk and frailty of the treated population, antithrombotic therapy after TAVI should be carefully evaluated. Diminishing ischaemic and bleeding complications remains the main challenge in these patients with further studies to be needed in this field.

关键词： Antiplatelets Antithrombotic Bleeding Stroke Transcatheter aortic valve implantation

Heroin-induced changes of CD34-positive rat thymus cells

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Health 2010年第8期2卷 957-961页

作者： Gentimi Fotini Perrea Despina Marinos Evangelos Konstandi ourania Katsorchis Theodoros 不详

Heroin is a well known opioid that causes al-terations to the immune system of a number of investigated animals. Only a few studies have explored the effect of heroin on the lymphocytes maturation. Thymocyte progenitors originate from haematopoietic stem cells in the bone marrow. The immature T-cells express neither CD4 nor CD8, and are therefore classed as double-negative (CD4-CD8-) cells. CD34 glycoprotein is the only defined marker of the immature T-lymphocytes. In this study we have investigated the changes induced in CD34+ rat thymocytes after heroin administration by immunofluores-cence in frozen rat thymus sections using the 4H11 monoclonal anti-CD34 antibody. There is a remarkable decrease in the number of CD34+ immature thymocytes when examined 1hour after last heroin injection and a small recover when examination took place 20 days after last injection. The above results suggest a major effect of heroin administration early in the ma- turation process of T lymphocytes probably by increasing the apoptotic cell death and the ne- gative consequences for the immune system responses.

关键词： Heroin Rat Thymus CD34