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The development and application of a multiplex short tandem repeat(STR)system for identifying subspecies,individuals and sex in tigers

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Integrative Zoology 2015年第4期10卷 376-388页

作者： Zheng-Ting ZOU olga v.UPHYRKINA Pavel FOMENKO Shu-Jin LUO College of Life Sciences Peking-Tsinghua Center for Life SciencesPeking UniversityBeijingChina Institute of Biology and Soil Sciences Far Eastern Branch Russian Academy of SciencesVladivostokRussia World Wide Fund for Nature–Russia Amur Branch VladivostokRussia Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMichiganUSA.

Poaching and trans-boundary trafficking of tigers and body parts are threatening the world’s last remaining wild *** of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship *** developed a multiplex genotyping system“tigrisPlex”to simultaneously assess 22 short tandem repeat(STR,or microsatellite)loci and a gender-identifying SRY gene,all amplified in 4 reactions using as little as 1 ng of template *** DNA samples used for between-run calibration,the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles,hence making it possible to identify subspecies via bi-parentally inherited *** applied“tigrisPlex”to 12 confiscated specimens from Russia and identified 6 individuals(3 females and 3 males),each represented by duplicated samples and all designated as Amur tigers(Panthera tigris altaica)with high *** STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products,fulfilling various conservation needs for identifying the origins of tiger samples.

关键词： Amur tiger genetic multiplex sexing short tandem repeat

The Fifth Edition of the General Catalogue of variable Stars:experiences in the constellation Centaurus

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Research in Astronomy and Astrophysics 2018年第7期18卷 93-100页

作者： Nikolay N.Samus Elena N.Pastukhova olga v.Durlevich Elena v.Kazarovets Institute of Astronomy Russian Academy of Sciences Sternberg Astronomical Institute Lomonosov Moscow University

We have recently announced that the General Catalogue of variable Stars enters the stage of its fifth, purely electronic edition （GCvS 5.1）. We have included 1408 variable stars in the constellation Centaurus in this new version, GCvS 5.1. Working on this revision, we applied current possibilities from data mining, suggested new variability types for many variable stars and found new light elements for a large number of periodic variables. This paper describes the work completed during the preparation of GCvS 5.1 for Centaurus and discusses in detail a number of the most astrophysically significant cases.

关键词： catalogs - techniques photometric - stars variables general - binaries eclipsing

Electrochemical performance of Klason lignin as a low-cost cathode-active material for primary lithium battery

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Journal of Energy Chemistry 2015年第3期24卷 346-352页

作者： Sergey v Gnedenkov Denis P Opra Ludmila A Zemnukhova Sergey L Sinebryukhov Iliya A Kedrinskii olga v Patrusheva valentin I Sergienko Institute of Chemistry Far Eastern BranchRussian Academy of Sciences Far Eastern Federal University Siberian State Technological University

A few classes of organic compounds are promising electrode-active materials due to their high power and energy densities,low cost,environmental friendliness,and *** the present work,the possibility of using Klason lignin extracted from buckwheat husks as a cathode-active material for a primary lithium battery has been investigated for the first *** reaction mechanism in the lithium/lignin electrochemical cell was suggested based on the deep galvanostatic discharge（up to 0.005 v） data and cyclic voltammetry *** dependence of the electrochemical behavior of the Klason lignin on the milling degree was *** maximum specific capacity of the lignin is equal to 600 m Ah g-1at a discharge current density of 75 μA *** effect of the thermal treatment of the Klason lignin cathode at250°C on the cell performance was *** was found that the discharge capacity of the cell increased by 30% in the range from 3.3 to0.9 v for the treated cathode *** results demonstrate the prospects of using Klason lignin-based electrochemical cells as low-rate primary power sources.

关键词： Klason lignin primary lithium battery organic electrode buckwheat husks

Controlled Wettability of Polymers via Environmental Crazing

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Chinese Journal of Polymer Science 2022年第10期40卷 1307-1314,I0012页

作者： Alena Yu.Yarysheva Larisa M.Yarysheva Fedor v.Drozdov olga v.Arzhakova Aziz M.Muzafarov Faculty of Chemistry Lomonosov Moscow State UniversityLeninskieGory 1-3Moscow 119991Russia Institute of Synthetic Polymer Materials RAS ul.Profsoyuznaya 70Moscow 117393Rusia

This work highlights the benefits of environmental crazing for the control over the hydrophilic-lipophilic balance of glassy amorphous and semicrystalline polymers:poly(ethylene terephthalate)(PET)and biodegradable polycaprolactone(PCL).Task-oriented encapsulation of diverse commercial and natural hydrophilic(polyethyleneoxide,polyvinylpyrrolidone,gelatin)and hydrophobic guest additives(perfluoroorgano siloxanes,water-repellent impregnation)into mesoporous host polymer(PET,PCL)matrixes allows targeted control over the wettability of the resultant nanocomposite *** on the nature of the guest additive,the properties of the modified polymers can be varied from hydrophilic to hydrophobic:contact angle(CA)of PET can be adjusted from 47_(⊥)/38_(||)to 130_(⊥)/117_(||)°(for initial PET,CA=82°)and CA of PCL from 40°to 114°(for initial PCL CA=75°).The unique benefits of environmental crazing for structural modification are related to the universal character of this approach which can be applied to a broad range of polymers and allows preparation of diverse high-performance materials with tunable hydrophilic-lipophilic balance.

关键词： Wettability Hydrophilic-hydrophobic balance Contact angle Environmental crazing Poly(ethylene terephthalate) Polycaprolactone

Hidden stage of intracranial hemorrhage in newborn rats studied with laser speckle contrast imaging and wavelets

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Journal of Innovative Optical Health Sciences 2015年第5期8卷 99-106页

作者： Alexey N.Pavlov Arkady S.Abdurashitov olga N.Pavlova valery v.Tuchin olga S.Sindeeva Sergey S.Sindeev Oxana v.Semyachkina-Glushkovskaya Department of Physics Saratov State University 83 Astrakhanskaya Str.Saratov 410012Russia Department of Biology Saratov State University 83 Astrakhanskaya Str.Saratov 410012Russia

Using the laser speckle contrast imaging and wavelet-based analyses,we investigate a latent("hidden")stage of the development of intracranial hemorrhages(ICHs)in newborn *** apply two measures based on the continuous wavelet-transform of blood flow velocity in the sagittal sinus,namely,the spectral energy in distinct frequency ranges and a multiscality degree characterizing complexity of experimental *** show that the wavelet-based multifractal formalism reveals changes in the cerebrovascular blood flow at the development of ICH.

关键词： Cerebral blood flow laser speckle contrast imaging stress wavelet-analysis complexity

The determination of white dwarf parameters in dwarf novae by optical spectral modeling

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Research in Astronomy and Astrophysics 2021年第7期21卷 37-40页

作者： Anastasiya Andreevna Dudnik Arina Alekseevna mitrofanova vladislav vladimirovich Shimansky Nikolay vladimirovich Borisov Nail Abdullovich Sakhibullin Kazan(volga Region)Federal University KazanRepublic of TatarstanRussia Special Astrophysical Observatory Russian Academy of ScienceNizhnii ArkhysKarachai-Cherkessian RepublicRussia

This paper presents the results of a model analysis of optical spectra and determination of the parameters of three individual SU UMa and WZ Sge type dwarf *** moderate resolution spectra of TY Psc,FL Psc and v455 And were obtained at the 6-m BTA of the SAO RAS in the low state of these systems with the determination of white dwarf *** theoretical spectra were calculated using the grid models of hydrogen dwarf atmospheres of white dwarfs by varying the parameters(T_(eff) and log g) to reach the best agreement with the observed *** highlight different effects of the parameters on the shape and intensity of the HI ***,it is possible to unambiguously determine T_(eff) and log g from the analysis of *** fundamental parameters of white dwarfs(M and R) were found by comparing the parameters of atmospheres with theoretical models of the internal *** obtained parameters of the primaries of TY Psc,FL Psc and v455 And are consistent with the average values for SU UMa and WZ Sge *** a result,we demonstrate the efficiency of the method for determining the parameters of such systems based on the analysis of a limited set of observed optical spectra.

关键词： techniques:spectroscopic stars:variables:dwarf novae stars:individual:TY Psc stars:individual:FL Psc stars:individual:v455 And

Factors associated with trabecular bone score in postmenopausal women with type 2 diabetes and normal bone mineral density

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World Journal of Diabetes 2022年第7期13卷 553-565页

作者： olga N Fazullina Anton I Korbut vadim v Klimontov Laboratory of Endocrinology Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and GeneticsSiberian Branch of Russian Academy of SciencesNovosibirsk 630060Russia

BACKGROUND Osteoporosis and type 2 diabetes(T2D)have been recognized as a widespread comorbidity leading to excess mortality and an enormous healthcare *** T2D,bone mineral density(BMD)may underestimate the risk of low-energy fractures as bone quality is *** was hypothesized that a decrease in the trabecular bone score(TBS),a parameter assessing bone microarchitecture,may be an early marker of impaired bone health in women with *** To identify clinical and body composition parameters that affect TBS in postmenopausal women with T2D and normal *** A non-interventional cross-sectional comparative study was *** eligible subjects were screened at tertiary referral *** women with T2D,aged 50-75 years,with no established risk factors for secondary osteoporosis,were ***,TBS and body composition parameters were assessed by dual-energy X-ray *** women with normal BMD,a wide range of anthropometric,general and diabetes-related clinical and laboratory parameters were evaluated as risk factors for TBS decrease using univariate and multivariate regression analysis and analysis of receiver operating characteristic(ROC)*** Three hundred twelve women were initially screened,176 of them met the inclusion criteria and underwent dual X-ray *** with reduced BMD were subsequently excluded;96 women with normal BMD were included in final *** them,43 women(44.8%)showed decreased TBS values(≤1.31).Women with TBS≤1.31 were taller and had a lower body mass index(BMI)when compared to those with normal TBS(Р=0.008 and P=0.007 respectively).No significant differences in HbA1c,renal function,calcium,phosphorus,alkaline phosphatase,PTH and 25(ОН)D levels were *** a model of multivariate linear regression analysis,TBS was positively associated with gynoid fat mass,whereas the height and androgen fat mass were associated negatively(all P<0.001).In a multiple logistic regres

关键词： Diabetes Osteoporosis Bone mineral density Trabecular bone score Obesity Body composition

Processing Methods of Alkaline Hydrolysate from Rice Husk

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Rice science 2017年第4期24卷 235-240页

作者： olga D.AREFIEvA Liudmila A.ZEMNUKHOvA Anastasia A.KOvSHUN Anna v.KOvEKHOvA Far Eastern Federal University Vladivostok 690950Russian Federation Institute of Chemistry Far-Eastern BranchRussian Academy of SciencesVladivostok 690022Russian Federation

This paper devoted to finding processing methods of alkaline hydrolysate produced from rice husk pre-extraction, and discusses alkaline hydrolysate processing schemed and disengagement of some products： amorphous silica of various quality, alkaline lignin, and water and alkaline extraction polysaccharides. Silica samples were characterized： crude （air-dried）, burnt （no preliminary water treatment）, washed in distilled water, and washed in distilled water and burnt. Waste water parameters upon the extraction of solids from alkaline hydrolysate dropped a few dozens or thousand times depending on the applied processing method. Color decreased a few thousand times, turbidity was virtually eliminated, chemical oxygen demanded about 20–136 times; polyphenols content might decrease 50% or be virtually eliminated. The most prospective scheme obtained the two following solid products from rice husk alkaline hydrolysate： amorphous silica and alkaline extraction polysaccharide. Chemical oxygen demand of the remaining waste water decreased about 140 times compared to the silica-free solution.

关键词： rice husk alkaline hydrolysate silica lignin polysaccharide

3D model of Svecofennian Accretionary Orogen and Karelia Craton based on geology,reflection seismics,magnetotellurics and density modelling:Geodynamic speculations

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Geoscience Frontiers 2020年第3期11卷 999-1023页

作者： Michael v.Mints victor N.Glaznev olga M.Muravina Elena Yu Sokolova Geological Institute RASMoscowRussia voronezh State University VoronezhRussia Schmidt Institute of Physics of the Earth RASMoscowRussia All-Russian Research Geological Oil Institute MoscowRussia

A 3D model of deep crustal structure of the Archaean Karelia Craton and late Palaeoproterozoic Svecofennian Accretionary Orogen including the boundary zone is *** model is based on the combination of data from geological mapping and reflection seismic studies,along profiles 1-EU,4 B,FIRE-1-2 a-2 and FIRE-3-3 a,and uses results of magnetotelluric soundings in southern Finland and northern Karelia.A seismogeological model of the crust and crust-mantle boundary is compared with a model of subhorizontal velocity-density layering of the *** TTG-type crust of the Palaeoarchaean and Mesoarchaean microcontinents within the Karelia Craton and the Belomorian Province are separated by gently dipping greenstone belts,at least some of which are *** structure of the crust was determined mainly by Palaeoproterozoic tectonism in the intracontinental settings modified by a strong collisional compression at the end of the *** insights into structure,origin and evolution of the Svecofennian Orogen are *** accretionary complex is characterized by inclined tectonic layering:the tectonic sheets,~15 km thick,are composed of volcanic-sedimentary rocks,including electro-conductive graphite-bearing sedimentary rocks,and electro-resistive granitoids,which plunge monotonously and consecutively *** reaching the level of the lower crust,the tectonic sheets of the accretionary complex lose their distinct *** the seismic reflection pattern they are replaced by a uniform acoustically translucent medium,where separate sheets can only be traced *** crust-mantle boundary bears a diffuse character:the transition from crust to mantle is recorded by the disappearance of the vaguely drawn boundaries of the tectonic sheets and in the gradual transition of acoustically homogeneous and translucent lower crust into transparent *** the effect of endogenic heat flow,the accretionary complex underwent high-temperatu

关键词： Reflection seismics Magnetotellurics Svecofennian accretionary Orogen Svecofennian Ocean 3D crustal model velocity-density layering