The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis
The RdDM Pathway Is Required for Basal Heat Tolerance in Arabidopsis作者机构：GMI-Gregor Mendel Institute of Molecular Plant Biology Austrian Academy of Sciences Dr. Bohr-Gasse 3 1030 Vienna Austria
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2013年第6卷第2期
页 面：396-410页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：supported by the Austrian Science Foundation supported by the GEN-AU program from the Austrian Federal Ministry of Science and Research
主 题：heat stress RNA-directed DNA methylation transposons read-through transcription.
摘 要：Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epige-netic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, his-tone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensi- tive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recover-ing from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway.
维普期刊数据库
