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Bioactive Compounds Recovery from Larrea tridentata by Green Ultrasound- Assisted Extraction

Phyton-International Journal of Experimental Botany 2024年第11期93卷 3091-3107页

作者： Muyideen Olaitan Bamidele Jose Sandoval-Cortes Maria Liliana Flores-Lopez olga berenice alvarez perez Monica Lizeth Chavez Gonzalez Cristobal Noe Aguilar Bioprocesses and Bioproducts Research Group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de CoahuilaSaltillo25070Mexico Centro de Investigacion e Innovacion Cientifica y Tecnologica Universidad Autonoma de CoahuilaSaltillo25070Mexico

Postharvest losses remain a signiﬁcant challenge,particularly in developing countries that have inadequate *** plants offer an eco-friendly and sustainable solution for managing diseases and pests in agri-cultural *** plants are rich in bioactive compounds,such as alkaloids,ﬂavonoids,terpenoids,phenolics,and essential oils,many of which have proven antimicrobial,antifungal,insecticidal,and antioxidant *** characteristics make medicinal plants viable candidates for postharvest disease and pest *** these,Larrea tridentata(creosote bush)is particularly notable for its bioactive compounds with strong antifungal *** potential applications include agriculture,food preservation,and *** study aimed to evaluate how different solvent mixtures affect the efﬁciency of ultrasound-assisted extraction,total polyphenol content,antioxidant capacity,and antifungal activity of *** leaves and ***ﬁndings revealed that the 60%ethanol ultrasound-assisted extract of *** leaves(ULL 60%)contained the highest concentration of bioactive compounds,including hydrolysable tannins(690.2 mg GAE/100 g)and condensed tan-nins(329.9 mg CE/100 g).All extracts demonstrated notable antioxidant activity in ABTS,DPPH,and FRAP assays,with ethanol-based extracts showing greater antioxidant potential than their aqueous *** terms of antifungal efﬁcacy,100%ethanol leaf extract exhibited the strongest inhibition against Fusarium oxyspor-um(60.03%),whereas 50%ethanol extract effectively inhibited Alternaria alternata(53.61%).Six major polyphe-nolic compounds were identiﬁed using reverse-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry(RP-HPLC-ESI-MS).These include quercetin,luteolin,3,4-dihydrox-yphenylethanol,elenolic acid,nordihydroguaiaretic acid(NDGA),and *** compounds are known to have antibacterial,antifungal and antioxidant propert

关键词： Phytopathogens bioactive compounds desert plants Fusarium oxysporum Alternaria alternata

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Development and characterization of whey protein films incorporated with tarbush polyphenols and candelilla wax

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Food Bioscience 2022年第1期45卷 1-7页

作者： olga B.alvarez-perez Janeth M.Ventura-Sobrevilla Cristian Torres-Leon Romeo Rojas-Molina Raúl Rodríguez-Herrera Miguel A.Aguilar-Gonzalez Cristobal N.Aguilar Bioprocesses and Bioproducts Research Group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de Coahuila25280SaltilloCoahuilaMexico School of Health Sciences Universidad Autonoma de Coahuila26090Piedras NegrasCoahuilaMexico School of Agronomy Research Center and Development for Food IndustryUniversidad Autonoma de Nuevo Leon6050General EscobedoNuevo LeonMexico Center for Research and Advanced Studies of the National Polytechnique Institute Parque Industrial Saltillo-Ramos ArizpeCoahuilaMexico

Edible films were prepared with whey protein incorporating candelilla wax,and glycerol in aqueous *** polyphenols were added as bioactive *** optimization based on the water vapor permeability(WVP)of the formulation was performed using a Box-Behnken *** films were characterized by the determination of WVP,moisture,solubility,color,antioxidant and antifungal *** electron microscopy(SEM)was also performed to observe the surface and cross-section *** lowest permeability values were found with concentrations of 1.27,0.3 and 0.5% of protein,wax and glycerol *** was proved that the addition of polyphenols into films causes a significant effect only on solubility and transparency *** films have suitable properties as a barrier for the goods they could be applied *** is proposed for use in foods such as fresh or minimally processed fruits and vegetables.

关键词： Edible films Whey protein Shelf life Candelilla wax Euphorbia antisyphilitica Fluorensia cernua polyphenols

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Education and Employment （2005-2009） A Comparative Analysis Between the Spanish and Foreign Working Population

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Chinese Business Review 2012年第4期11卷 411-423页

作者： Ma olga Gonzalez Morales Flora Ma Diaz perez Jose Antonio alvarez Gonzalez University of La Laguna S/C Tenerife Spain

Spain became a net receiver country of a high number of foreigners entering the labour market in the first decade of the 21 st century. The ensuing economic crisis, beginning in 2007, led to the destruction of a large number of companies and jobs. The objective of this paper is to analyze the relationship between educational level, employment and economic activity, in the Spanish and foreign working population in the Spanish labour market and the impact that the economic crisis is having on employment and self-employment. The hypotheses considered have been as followed--HI： The economic crisis mainly affects the less educated working population, both Spanish and foreign; H2： Self-employment is characterized by being an initiative of a working population with low educational levels for both population groups （Spanish and foreign）; H3： Employment is related to the productive economic structure. Micro-data from the Labour Force Survey 2009 （EPA in Spanish） has been used, compiled by Spains National Statistics Office （INE in Spanish）, and it has been applied Multiple Correspondence Analysis （MCA） to detect possible associations among the study variables （nationality, level of education, salaried employment, self-employment and economic activity）. The results show significant differences between both groups： Spanish and foreign working population. The study also shows how the crisis affects employment, and the important role played by the educational level of the working population, especially foreigners employed in certain economic activities.

关键词： working population nationality salaried employment self-employed level of education activity sector economic crisis

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XAANTAL2 （AGL14） Is an Important Component of the Complex Gene Regulatory Network that Underlies Arabidopsis Shoot Apical Meristem Transitions

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Molecular Plant 2015年第5期8卷 796-813页

作者： Rigoberto V. perez-R.u.iz berenice Garcia-Ponce Nayelli Marsch-Martinez Yamel Ugartechea-Chirino Mitzi Villajuana-Bonequi Stefan de Folter Eugenio Azpeitia Jose Davila-Velderrain David Cruz-Sanchez Adriana Garay-Arroyo Maria de la Paz Sanchez Juan M. Estevez-Palmas Elena R. alvarez-Buylla Instituto de Ecologia Universidad Nacionat Autonoma de Moxico 3er Circuito Exterior s/no Junto al Jardin Bot~nico and Centro de Ciencias de la ComplejidadCiudad Universitaria Coyoacan 04510 Mexico D.F. Mexico Laboratorio Nacional de Genemica para la Biodiversidad (Langebio) Centro de Investigaci6n y de Estudios Avanzados del Instituto Polit~cnico Nacional(CINVESTAV-IPN) Km. 9.6 Carretera Irapuato - Leen AP 629 36821 Irapuato Guanajuato Mexico University of California 431 Koshland Hall Berkeley CA 94720 USA These authors contributed equally to this article. present address: Departamento de Biotecnologia y Biquimica Centro de )nvestigaci6n y de Estudios Avanzados del Instituto Polit6cnico Nacional(CINVESTAV-IPN) Km. 9.6 Libramiento Norte Carr. Irapuato-Leen 36821 Irapuato Guanajuato Mexico present address: Max Planck Institute for Plant Breeding Research D-50829 Cologne Germany Present address: INRIA Project-Team Virtual Plants/CIRAD/INRA UMR AGAP Campus St Priest - BAT 5 CC 05018 860 rue de St Priest 34095 Montpellier Cedex5 France

In Arabidopsis thaliana, multiple genes involved in shoot apical meristem （SAM） transitions have been char- acterized, but the mechanisms required for the dynamic attainment of vegetative, inflorescence, and floral meristem （VM, IM, FM） cell fates during SAM transitions are not well understood. Here we show that a MADS-box gene, XAANTAL2 （XAL2/AGL14）, is necessary and sufficient to induce flowering, and its regula- tion is important in FM maintenance and determinacy, xal2 mutants are late flowering, particularly under short-day （SD） condition, while XAL2 overexpressing plants are early flowering, but their flowers have vege- tative traits. Interestingly, inflorescences of the latter plants have higher expression levels of LFY, AP1, and TFL1 than wild-type plants. In addition we found that XAL2 is able to bind the TFL1 regulatory regions. On the other hand, the basipetal carpels of the 35S：：XAL2 lines lose determinacy and maintain high levels of WUS expression under SD condition. To provide a mechanistic explanation for the complex roles of XAL2 in SAM transitions and the apparently paradoxical phenotypes of XAL2 and other MADS-box （SOCl, AGL24） over- expressors, we conducted dynamic gene regulatory network （GRN） and epigenetic landscape modeling. We uncovered a GRN module that underlies VM, IM, and FM gene configurations and transition patterns in wild- type plants as well as loss and gain of function lines characterized here and previously. Our approach thus provides a novel mechanistic framework for understanding the complex basis of SAM development.

关键词： XAL2/AGL14 MADS-box TFL1 SAM transitions floral reversion gene regulatory networks,epigenetic landscape modeling

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Tomato waste as a bioresource for lycopene extraction using emerging technologies

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Food Bioscience 2022年第5期49卷 99-108页

作者： J.Y.M´endez-Carmona J.A.Ascacio-Valdes olga B.alvarez-perez Ayerim Y.Hern´andez-Almanza Nathiely Ramírez-Guzman Leonardo Sepúlveda Miguel A.Aguilar-Gonz´alez Janeth M.Ventura-Sobrevilla Crist´obal N.Aguilar School of Chemistry Universidad Aut´onoma de CoahuilaSaltilloMexico School of Biological Sciences Universidad Aut´onoma de CoahuilaTorre´onMexico Center for Interdisciplinary Studies and Research Universidad Aut´onoma de CoahuilaSaltillo25280Mexico Center for Research and Advanced Studies of the National Polytechnic Institute Ramos ArizpeCoahuilaMexico School of Health Sciences Universidad Aut´onoma de CoahuilaPiedras NegrasMexico

Background:Tomato is the second most important vegetable around the *** processing of the fruit generates waste such as damaged tomatoes,pulp,skin,seeds,and tomato-derived products that contain valuable biomolecules with functional and technological *** fruit extracts,mainly from waste,possess bioactive molecules,such as lycopene,with important biological applications in some *** and approach:The objective of this review is to analyze,discuss and synthesize the technologies that have been used for the extraction and recovery of the main lycopene present in tomato *** revised methodologies expose valuable results in the number of carotenoids recovered through the application of solvents,microwave-assisted,ultrasound-assisted,enzymatic-assisted,extraction at high pressure,and supercritical ***,the challenges continue concerning the quality of extracted compounds,economic accessibility,industrial reproducibility,and environmental *** findings and conclusions:The tomato waste contains important bioactive molecules,which are of interest in the food,pharmacological,biotechnological,and cosmetic *** information revised in this contribution pretends to support new research lines concerning the formulation of novel tomato waste bio-based functional ***,it is necessary to further analyze the new extraction and recovery conditions to keep the functional properties of this by-product.

关键词： Tomato waste Carotenoids Techniques for extraction Lycopene

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Gut microbiota in a population highly affected by obesity and type 2 diabetes and susceptibility to COVID-19

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World Journal of Gastroenterology 2021年第41期27卷 7065-7079页

作者： Jaime García-Mena Karina Corona-Cervantes Daniel Cuervo-Zanatta Tizziani Benitez-Guerrero JuanManuel Vélez-Ixta Norma Gabriela Zavala-Torres Loan Edel Villalobos-Flores Fernando Hernández-Quiroz Claudia perez-Cruz Selvasankar Murugesan Fernando Guadalupe Bastida-González Paola berenice Zárate-Segura Departamento de Genética y Biología Molecular Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Departamento de Genética y Biología Molecular and Departamento de Farmacología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Departamento de Farmacología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Department of Mother and Child Health SIDRA MedicineDoha 26999Qatar Laboratorio Estatal de Salud Pública del Estado de México ISEMToluca de Lerdo 50180Estado de MéxicoMexico Escuela Superior de Medicina Instituto Politécnico NacionalMexico City 11340CDMXMexico

Coronavirus disease 2019(COVID-19)is a disease produced by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and it is currently causing a catastrophic pandemic affecting humans *** disease has been lethal for approximately 3.12 million people around the world since January ***,among the most affected countries,Mexico ranks third in deaths after the United States of America and *** the high number of deceased people might also be explained by social aspects and lifestyle customs in Mexico,there is a relationship between this high proportion of deaths and comorbidities such as high blood pressure(HBP),type 2 diabetes,obesity,and metabolic *** official epidemiological figures reported by the Mexican government have indicated that 18.4%of the population suffers from HBP,close to 10.3%of adults suffer from type 2 diabetes,and approximately 36.1%of the population suffers from *** in the gut microbiota(GM)have been associated with these diseases and with COVID-19 severity,presumably due to inflammatory *** data about the association between GM dysbiosis and metabolic diseases could suggest that the high levels of susceptibility to SARSCoV-2 infection and COVID-19 morbidity in the Mexican population are primarily due to the prevalence of type 2 diabetes,obesity,and metabolic syndrome.

关键词： SARS-CoV-2 COVID-19 High blood pressure Hypertension Type 2 diabetes Obesity Metabolic syndrome Gut microbiota Immunity

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Management of cancer patients during COVID-19 pandemic at developing countries

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World Journal of Clinical Cases 2020年第16期8卷 3390-3404页

作者： Jaime González-Montero Guillermo Valenzuela Mónica Ahumada olga Barajas Luis Villanueva Department Faculty of MedicineUniversity of ChileSantiago 70058Chile Department Hospital Clinico Universidad de Chile and Clínica DávilaChile Department Hospital Clinico Universidad de Chile and Fundación Arturo López-PérezChile Department Hospital Clínico Universidad de Chile and Fundación Arturo López-PerezChile

Cancer patient care requires a multi-disciplinary approach and multiple medical and ethical *** care during a pandemic health crisis requires prioritising the use of resources for patients with a greater chance of survival,especially in developing *** coronavirus disease 2019 crisis has generated new challenges given that cancer patients are normally not prioritised for admission in critical care ***,the development of new cancer drugs and novel adjuvant/neoadjuvant protocols has dramatically improved the prognosis of cancer patients,resulting in a more complex decision-making when prioritising intensive care in pandemic *** this context,it is essential to establish an effective and transparent communication between the oncology team,critical care,and emergency units to make the best decisions,considering the principles of justice and ***,cancer treatment protocols must be adapted to prioritise according to oncologic response and *** technologies are powerful tools to optimise cancer care during pandemics,and we must adapt quickly to this new scenario of clinical care and *** this new challenging pandemic scenario,multi-disciplinary work and effective communication between clinics,technology,science,and ethics is the key to optimising clinical care of cancer patients.

关键词： Cancer Oncology Pandemic COVID-19 SARS-CoV-2

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When Information Does Not Translate into Knowledge. Ebola and Hemorrhagic Fevers Knowledge among Primary Care Physicians and Nurses

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Open Journal of Preventive Medicine 2015年第3期5卷 122-127页

作者： Lluis Valerio olga perez-Quilez Silvia Roure Elisabeth Fructuoso Itziar Amilibia Nemesio Moreno Lydia Sanudo Cristina Bocanegra Octavi Martinez-Cuevas North Metropolitan International Health Unit(PROSICS) Institut Catalade la SalutSanta Coloma de GramenetCataloniaSpain North Metropolitan Pimary Care Service Institut Catalade la SalutBadalonaCataloniaSpain North Metropolitan Primary Care Service Epidemiology and Technical DepartmentInstitut Catalàde la SalutSabadellCataloniaSpain

After the first secondarily-transmitted ebola case in Spain, a wave of divergent opinions flooded mass and sanitary media. Very few of these opinions, however, came from experts on epidemiology or hemorrhagic fevers. This observational study aimed to assess the specific knowledge of Primary Care physicians and nurses about ebola and hemorrhagic fevers by means of analyzing the results obtained from a 5-item self-reported questionnaire dealing on hemorrhagic fevers basic knowledge. Validity and reliability of questionnaire were confirmed by a pilot study. The participants were 138 family doctors and nurses from the 64 public Primary Care centers sited in the North Metropolitan Area of Barcelona (1,400,000 inhab;Catalonia, Spain) taking part in training-the-trainers ebola workshops. Overall, there were 117 (84.8%) respondents out from 138 workshop participants;of them were physicians 61 (51.2%). The main age was 46.7 (8.8) years;stating previous specific knowledge on hemorrhagic fevers 39 (33.3%). On the whole, up to 92 (78.6%) of respondents shown a poor knowledge. Previous specific formation was significantly and independently associated with having proper knowledge (p < 0.001);OR = 8.6 (CI 95%: 3.199 - 23.623). In summary, confusion that accompanied the single secondary-transmitted ebola case in Spain could probably be explained by the existence of a serious gap on hemorrhagic fevers knowledge. More accurate, scientific and formally-presented information should be provided to Primary Care physicians and nurses.

关键词： Ebola Haemorrhagic Fevers Primary Care Spain Nursing Knowledge

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Development of a recurrent pleural effusion in a patient with pulmonary arterial hypertension treated with imatinib

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Case Reports in Clinical Medicine 2012年第2期1卷 38-41页

作者： olga M. Fortenko Lana Melendres-Groves Alice Richter Xiaobo Liao Edda Spiekerkoetter Roham Zamanian Vinicio A. De Jesus perez Stanford University Medical Center Stanford USA University of New Mexico Albuquerque USA

Pulmonary arterial hypertension (PAH) is a devastating disease associated with progressive elevation in pulmonary pressures that eventually leads to chronic right heart failure and death. At present, agents with vasodilatory properties are being used to palliate the symptoms associated with PAH but there is a need for therapies that can prevent or even reverse established disease. Several lines of evidence have suggested that tyrosine kinase inhibitors like imatinib may have a role in reducing progression and improving outcomes in these patients, but their side effect profile is unclear. We present a case of a 55-year-old female with PAH secondary to connective tissue disease treated with triple PAH specific therapy and compassionate-use imatinib who developed a massive right pleural effusion. Despite multiple therapeutic thoracentesis and aggressive diuresis, the pleural effusion continued to re-accumulate necessitating chest tube placement. Resolution of the pleural effusion was finally achieved after imatinib was held, arguing that the patient’s presentation likely was a drug-related event. We believe that our case highlights a serious adverse reaction to imatinib therapy and stresses the need for more studies to evaluate the safety profile of this medication in patients with PAH.

关键词： Pulmonary Hypertension Imatinib Tyrosine Kinase Inhibitors Pleural Effusion

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