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Does granulocyte-colony stimulating factor administration induce damage or repair response in schistosomiasis?

World Journal of Hepatology 2010年第12期2卷 434-441页

作者： Lobna Y Ghanem Uta Dahmen olaf dirsch Mona MF Nosseir Soheir S Mahmoud Wafaa AF Mansour Department of Electron Microscope Theodor Bilharz Research Institute Department of General Visceral and Transplantation SurgeryUniversity Hospital Essen Division of Pathology German Heart Institute Department of Pathology Theodor Bilharz Research Institute Department of Parasitology Theodor Bilharz Research Institute Department of Immunology Theodor Bilharz Research Institute

AIM:To introduce Granulocyte-colony stimulating factor (G-CSF) as a new therapeutic modality for schistosomiasis through stem cell mobilization,immunomodulation or fibrosis remodeling. METHODS:In this study,a 5 d course of human recombinant G-CSF (100 μg/kg sc) was applied to Schis-tosoma mansoni-infected mice at different stages of disease (5 d before infection as well as 3,5 and 7 wk post-infection). The animals were sacrificed at 10 d as well as 4,6 and 8 wk post infection. Mice were examined for:(1) Total leukocyte count which is an accepted surrogate marker for the stem cell mobilization into the circulation; (2) Egg count in intestine and liver tissue to assess the parasitic load; and (3) Histopathological changes in Hx/E and Masson trichrome stained sections as well as collagen content in Sirius redstained liver sections to determine the severity of liver fibrosis. RESULTS:Mice developed leukocytosis. The egg load and the number of granulomas were not affected by the G-CSF treatment but there was an obvious change in the composition of granulomas towards an increased cellularity. Moreover,fibrosis was significantly decreased in treated groups compared to untreated animals (collagen content either preinfection or at 3 and 5 wk post infection:5.8 ± 0.5,4.7 ± 0.5,4.0 ± 0.7 vs 8.2 ± 0.9; P ≤ 0.01). CONCLUSION:Although G-CSF did not cause direct elimination of the parasite,it enhanced granulomatous reaction and reduced the fibrosis. Further investigation of the underlying mechanisms of these two actions is warranted.

关键词： Schistosomiasis Granulocyte-colony stimulating factor Periovular granuloma Fibrosis Immunomodulation Stem cell mobilization

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Administration of granulocyte colony stimulating factor after liver transplantation leads to an increased incidence and severity of ischemic biliary lesions in the rat model

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World Journal of Gastroenterology 2006年第31期12卷 5021-5027页

作者： olaf dirsch Haidong Chi Yuan Ji Yan Li Gu Christoph E Broelsch Uta Dahmen Institute of Pathology University Hospital Cologne Joseph-Stelzmann Strasse 9 D-50931 Cologne Germany Department of General Visceral and Transplantation Surgery University Hospital Essen Hufelandstrasse 55 D-45122 Essen Germany

AIM： Recently it has been reported that granulocyte colony stimulating factor （G-CSF） can induce hypercoagulability in healthy bone marrow donors. It is conceivable that the induction of a prothrombotic state in a recipient of an organ graft with already impaired perfusion might cause further deterioration in the transplanted organ. This study evaluated whether G-CSF treatment worsens liver perfusion following liver transplantation in the rat model. METHODS： A non-arterialized rat liver transplantation model was employed to evaluate the effect of G-CSF treatment on the liver in a syngeneic and allogeneic strain combination. Study outcomes included survival time and liver damage as investigated by liver enzymes and liver histology. Observation times were 1 d, 1 wk and 12 wk. RESULTS： Rats treated with G-CSF had increased incidence and severity of biliary damage following liver transplantation. In these animals, hepatocellular necrosis was accentuated in the centrilobular region. These lesions are indicative of impaired perfusion in G-CSF treated animals. CONCLUSION： G-CSF should be used with caution in recipients of liver transplantation, as treatment might enhance preexisting, undetected perfusion problems and ultimately lead to ischemia induced biliary complications .

关键词： Granulocyte colony stimulating factor Ischemic biliary lesions Hypercoagulability Liver transplantation

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Ischemic preconditioning protects against liver ischemia/reperfusion injury via heme oxygenase-1-mediated autophagy

Ischemic preconditioning protects against liver ischemia/rep...

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第九届全国免疫学学术大会

作者： Anding Liu Haoshu Fang Yan Yang Shenpei Liu Dongliang Yang Mengji Lu olaf dirsch Uta Dahmen Experimental Medicine Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Experimental Transplantation Surgery Department of GeneralVisceral and Vascular SurgeryFriedrich-Schiller-University Jena Department of Pathophysiology Anhui Medical University Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Institute of Virology University Hospital of EssenUniversity of Duisburg-Essen EssenEssen45122 Germany

Background:Ischemic preconditioning(IPC)exerts a protective effect in hepatic ischemia/reperfusion(I/R)*** exact mechanism of IPC action remains largely *** studies suggest that autophagy plays an important role in protecting against I/R ***,the role of autophagy in IPC-afforded protection and its regulatory mechanisms in liver I/R injury remain poorly *** study was designed to determine whether IPC could protect against liver I/R injury via heme oxygenase-1(HO-1)-mediated ***:IPC was produced by 10 min of ischemia followed by 10 min reperfusion prior to 60 min of *** a rat model of hepatic I/R injury,rats were pretreated with wortmannin or rapamycin to evaluate the contribution of autophagy to the protective effects of ***-1 was inhibited with tin protoporphyrinⅨ(SnPP).In a mouse model of hepatic I/R injury,autophagy or HO-1 was inhibited with vacuolar protein sorting 34(Vps34)small interfering RNA(siRNA)orHO-1 siRNA,***:IPC ameliorated liver I/R injury,as indicated by lower serum aminotransferase levels,lower hepatic inflammatory cytokines,and less severe I/R-associated histopathologic *** treatment induced autophagy activation,as indicated by an increase of LC3-Ⅱ,degradation of p62 and accumulation of autophagic vacuoles in response to I/R *** IPC-induced autophagy was inhibited with wortmannin in rats or Vps34-specific siRNA in mice,liver I/R injury was worsened,while rapamycin treatment increased autophagy and mimicked the protective effects of ***,IPC increased HO-1 *** inhibition of HO-1 with tin protoporphyrinⅨ(SnPP)in rats or HO-1-specific siRNA in mice decreased IPC-induced autophagy and diminished the protective effects of IPC against I/R ***:IPC protects against liver I/R injury,at least in part,via HO-1-mediated autophagy.

关键词： Autophagy Ischemic Preconditioning Hepatoprotection Liver I/R injury Heme oxygenase-1

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冷缺血对大鼠小体积肝移植肝再生的影响

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岭南现代临床外科 2006年第3期6卷 170-172页

作者：何清陈燕涛王捷谷艳丽 Uta Dahmen olaf dirsch 中山大学附属第二医院SICU 广州510120 中山大学附属第二医院骨外科广州510120 中山大学附属第二医院普外科广州510120 德国Essen大学医院普通与移植外科德国essen45122 德国KWln大学医院病理科德国kln50924

目的探讨冷缺血对大鼠小体积肝移植术后肝再生的影响。方法本组在2002年9月～2004年8月利用大鼠肝总量30%原位肝移植模型,实验分为肝总量70%肝切除组(C组)和冷缺血1、3、5h组(E1、E2、E3组),观察1w生存率及肝重量/受体原肝重量比值(EGW/... 详细信息

目的探讨冷缺血对大鼠小体积肝移植术后肝再生的影响。方法本组在2002年9月～2004年8月利用大鼠肝总量30%原位肝移植模型,实验分为肝总量70%肝切除组(C组)和冷缺血1、3、5h组(E1、E2、E3组),观察1w生存率及肝重量/受体原肝重量比值(EGW/RLW),并检测术后1、2、3、7d肝细胞增殖活性及肝组织学变化。结果E1组1w生存率及EGW/RLW分别达100%和95%,均明显高于E2、E3组(P均0.05);组织学检查见E1组肝细胞核分裂明显活跃。结论冷缺血1h的小体积供肝和70%肝切除后肝脏具有同样的增殖活性,仅增殖高峰稍晚;冷缺血超过3h严重影响小体积供肝的再生能力和受者的存活率。

关键词：冷缺血小体积肝移植肝再生

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Novel mouse models with a woodchuck hepatitis virus(WHV)transgene show sex-dependent WHV replicative activity and development of spontaneous immune responses to WHV proteins

Novel mouse models with a woodchuck hepatitis virus(WHV)tran...

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中华医学会第十六次全国病毒性肝炎及肝病学术会议

作者： Zhongji Meng Ejuan Zhang Anna D.Kosinska Zhiyong Ma Xiaoyong Zhang Tianlun Zhou Jun Wu Uta Dahmen olaf dirsch Dongliang Yang Michael Roggendorf Mengji Lu Department of Infectious Diseases Taihe HospitalHubei University of Medicine Institute of Virology University Hospital of EssenUniversity Duisburg-Essen Fox Chase Cancer Center Department of Infectious Disease Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of General Visceral and Vascular SurgeryUniversity Hospital of JenaFriedrich Schiller-University Jena Institute of Pathology University Hospital of JenaFriedrich Schiller-University Jena

Background & Aims The woodchuck model is an informative model for studies on hepadnaviral infection. In this study,woodchuck hepatitis virus(WHV) transgenic(Tg) mouse models based on C57BL/6 mice were established to study the pathogenesis associated with hepadnaviral *** C57BL/6 mice were used to establish WHV Tg *** replication intermediates were detected by Southern blotting. DNA vaccines against WHV proteins were applied by intramuscular ***-specific immune responses were analyzed by flow cytometry and *** Two lineages of WHV Tg mice harboring the WHV wild-

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大鼠小体积肝移植后肝细胞再生的研究

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岭南现代临床外科 2005年第3期5卷 233-235页

作者：何清陈燕涛张红卫谷艳丽 Uta Dahmen olaf dirsch 王捷中山大学附属第二医院SICU 广州510120 中山大学附属第二医院骨外科广州510120 中山大学附属第二医院普外科广州510120 德国Essen大学医院普通与移植外科德国essen45122 德国Kln大学医院病理科德国kln50924

目的探讨小体积肝移植术后肝再生的情况。方法建立大鼠30%原位肝移植模型,实验分为肝切除(PH)组、全肝移植(OLT)组和30%小体积肝移植(30%POLT)组,观察1w生存率,并于术后1、2、3、7d检测肝细胞增殖活性、肝功能和肝组织学变化。结果各组1... 详细信息

目的探讨小体积肝移植术后肝再生的情况。方法建立大鼠30%原位肝移植模型,实验分为肝切除(PH)组、全肝移植(OLT)组和30%小体积肝移植(30%POLT)组,观察1w生存率,并于术后1、2、3、7d检测肝细胞增殖活性、肝功能和肝组织学变化。结果各组1w生存率均为100%;30%POLT组术后2d达到增殖高峰,峰值与PH组无差异(P>0.05);其肝功能酶学指标在术后1、3d明显升高,组织学见肝细胞核分裂极其活跃。结论冷缺血1h的30%供肝和肝切除后肝脏具有同样的增殖活性,仅增殖高峰稍晚;较短的冷缺血时间以及熟练的手术技术可能对小体积供肝的再生起重要作用。

关键词：小体积肝移植肝再生大鼠

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Influence of rotating wakes on separation in turbine exhaust diffusers

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Journal of Thermal Science 2008年第1期17卷 42-49页

作者： olaf Sieker Joerg R.Seume Department of Turbomachinery and Fluid Dynamics Leibniz University HannoverAppelstr.9DE-30167 HannoverGermany Dipl.-Ing. Department of Turbomachinery and Fluid Dynamics Leibniz University HannoverAppelstr.9DE-30167 HannoverGermany

Highly efficient turbine exhaust diffuser cannot be designed without taking into account the unsteady interactions with the last rotating row of the turbine. Former investigations described in the literature show a very high potential compared to that of other parts of turbomachines for improving the diffuser. A scale model of a typical gas turbine exhaust diffuser is investigated experimentally. To investigate the influence of rotating wakes, measurements without a spoke wheel as well as measurements with a variable-speed rotating cylindrical spoke wheel with 2 mm- or 10 mm-spokes simulating turbine rotor wakes were made. Miniaturized 3-hole pneumatic probes as well as a 2D-Laser-Doppler-Velocimeter （LDV） were used to investigate velocity profiles. 122 static pressure tapings were used to measure several axial and circumferential static pressure distributions. Without a spoke-wheel the annular diffuser separates at the shroud for all swirl configurations. For the measurements with the 2 mm spoke wheel, the separating diffuser was unstable while keeping the test rig operating parameters constant. For a non-rotating 10 mm spoke wheel and at rotational speeds less than 1,000 rpm, the annular diffuser separated at the shroud. Increasing the rotational speed of the 10mm spoke wheel, flow did not separate at the shroud and much higher pressure recovery than without spoke wheel has achieved.

关键词： turbine exhaust diffuser rotating wakes separation LDV

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Impacts of prenatal nutrition on animal production and performance： a focus on growth and metabolic and endocrine function in sheep

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Journal of Animal Science and Biotechnology 2018年第1期9卷 10-23页

作者： Prabhat Khanal Mette olaf Nielsen Department of Veterinary and Animal Sciences Faculty of Health and Medical SciencesUniversity of Copenhagen Gronnegardsvey 31st floorDK-1870 Frederiksberg CDenmark Department of Nutrition Faculty of MedicineTransgenic Animal and Lipid StorageNorwegian Transgenic Centre(NTS)University of OsloOsloNotway

The concept of foetal programming（FP） originated from human epidemiological studies, where foetal life nutrition was linked to health and disease status later in life. Since the proposal of this phenomenon, it has been evaluated in various animal models to gain further insights into the mechanisms underlying the foetal origins of health and disease in humans. In FP research, the sheep has been quite extensively used as a model for humans. In this paper we will review findings mainly from our Copenhagen sheep model, on the implications of late gestation malnutrition for growth, development, and metabolic and endocrine functions later in life, and discuss how these implications may depend on the diet fed to the animal in early postnatal life. Our results have indicated that negative implications of foetal malnutrition, both as a result of overnutrition and, particularly, late gestation undernutrition, can impair a wide range of endocrine functions regulating growth and presumably also reproductive traits. These implications are not readily observable early in postnatal life, but are increasingly manifested as the animal approaches adulthood. No intervention or cure is known that can reverse this programming in postnatal life. Our findings suggest that close to normal growth and slaughter results can be obtained at least until puberty in animals which have undergone adverse programming in foetal life, but manifestation of programming effects becomes increasingly evident in adult *** to the risk of transfer of the adverse programming effects to future generations, it is therefore recommended that animals that are suspected to have undergone adverse FP are not used for reproduction. Unfortunately, no reliable biomarkers have as yet been identified that allow accurate identification of adversely programmed offspring at birth,except for very low or high birth weights, and, in pigs, characteristic changes in head shape（dolphin head）. Future efforts should be therefo

关键词： Adipose tissue Endocrine function Foetal programming Metabolic function Sheep

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Effect of Different Calcination Temperatures and Post Annealing on the Properties of Acetic Acid Based Sol-Gel (Na_(0.5)K_(0.5))NbO_3(NKN) Thin Films

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Journal of Materials Science & Technology 2013年第2期29卷 142-148页

作者： Sebastian Wiegand Stefan Flege olaf Baake Wolfgang Ensinger Materials Analysis Group Department of Materials and Geosciences Technische Universitt Darmstadt Petersenstrasse 23 64287 Darmstadt Germany

（Na0.5K0.5）NbO3 （NKN） lead free thin films were synthesized by means of an acetic acid based sol-gel process on Pt/Ti/SiO2/Si substrates. Na-acetate, K-acetate and Nb-pentaethoxide were used as metal precursors and acetic acid as the solvent. The effect of different calcination temperatures on the properties of the NKN films was investigated by X-ray diffraction, scanning electron microscopy, leakage current and hysteresis measurements. Low calcination temperatures led to low currents at high electric fields whereas high calcination temperatures led to low currents at low electric fields. Based on these findings calcination at low temperature was combined with a post annealing treatment. Low leakage currents of 4×10^-4 A/cm2 at 150 kV/cm and 2Pr and 2Ec values of 28 μC/cm2 and 150 kV/cm, respectively, could be obtained. All films were single phase NKN with random crystal orientations and no crack or pore formation was visible on the surface.

关键词： Sol-gel process (Na0.5K0.5)NbO3 Thin film Perovskite Lead-free Piezo material

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Synthesis and Characterization of(Na_(0.5)K_(0.5))NbO_3(NKN) Thin Films Formed by a Diol-based Sol-gel Process

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Journal of Materials Science & Technology 2012年第6期28卷 500-505页

作者： Sebastian Wiegand Stefan Flege olaf Baake Wolfgang Ensinger Technische Universitt Darmstadt Department of Materials and GeosciencesMaterials Analysis GroupPetersenstrasse 2364287 DarmstadtGermany

Lead-free （***0.5）Nb03 （NKN） thin films were fabricated by spin coating on Pt/Ti/SiO2/Si substrates by a diol-based sol-gel process. Na-acetate, K-acetate, Nb-pentaethoxide and 1,3 propanediol were used to prepare the NKN precursor solution. Thermal analysis showed two characteristic temperatures of 360 and 600℃. Based on these temperatures, a heat treatment program with pyrolysis at 360℃ and calcination at 600℃ after every layer was used. To avoid inhomogeneities and secondary phases, an excess of sodium and potassium was necessary. To evaluate the proper excess amount of sodium and potassium secondary ion mass spectrometry （SIMS） lateral element maps and X-ray diffraction （XRD） patterns were recorded. An excess amount of 20% led to homogeneous distribution of the elements and to single phase perovskite NKN films with random crystal orientation. Scanning electron microscopy （SEMI images showed a pore free surface with 100 nm grains. The leakage current measurements showed a current of lx10-3 A/cm2 at 150 kV/cm.

关键词： Sol-gel process (Nao.sK0.5)NbO3 Thin film Perovskite Lead-free Piezo material

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