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BIoCELL 2024年第8期48卷 1197-1208页

作者： MARYNA TKACHENKo ANAToLII oNISHCHENKo LILIYA TRYFoNYUK DMYTRo BUToV KATERYNA KoT VIKToRIIA novikova LIWEI FAN VoLoDYMYR pRoKopIUK YURII KoT ANToN TKACHENKo Department of Internal Medicine No.2 Clinical Immunology and AllergologyKharkiv National Medical UniversityKharkiv61022Ukraine Research Institute of Experimental and Clinical Medicine Kharkiv National Medical UniversityKharkiv61022Ukraine Institute of Health National University of Water and Environmental EngineeringRivne61022Ukraine Department of Infectious Diseases and phthisiology Kharkiv National Medical UniversityKharkiv61022Ukraine Biochemistry Department V.N.Karazin Kharkiv National University Ministry of Education and Science of UkraineKharkiv61022Ukraine Department of Chemistry BiochemistryMicrobiology and Food HygieneState Biotechnological UniversityKharkiv61022Ukraine Clinical Research Innovation Lab Floor 26 Block D Qidi Technology Building 8Tsinghua Technology ParkBeijing100084China Department of Cryobiochemistry Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of UkraineKharkiv61015Ukraine

Introduction:Human chemerin is an adipokine that regulates chemotaxis,inflammation,and glucose *** addition,accumulating evidence suggests that chemerin promotes apoptosis,autophagy,and ***,there are no data on its impact on *** current study aimed to analyze the effects of human active Glu^(21)-Ser^(157) chemerin on eryptosis in *** and Methods:Human chemerin 0-2-10-50μg/mL was incubated for 24 h with human erythrocytes(hematocrit 0.4%)obtained from eight healthy *** cytometry-based determination of phospholipid scrambling,reactive oxygen species(RoS)production,and intracellular Ca^(2+)levels was *** supplement data on RoS and Ca^(2+)signaling in chemerin-mediated eryptosis,incubation in the presence or absence of antioxidants vitamin C and N-acetylcysteine and Ca^(2+)-binding agent EGTA was carried out,*** microscopy-based techniques were used to detect reactive nitrogen species(RNS)generation,involvement of caspase-3 and caspase-8,as well as the state of lipid order in cell membranes of erythrocytes exposed to human Glu^(21)-Ser^(157) ***:our observations suggest that human Glu^(21)-Ser^(157) chemerin had no impact on eryptosis parameters at 2μg/***,chemerin stimulated phosphatidylserine externalization,RoS production,and Ca^(2+)accumulation at higher concentrations suggesting activation of ***^(2+)uptake turned out to be at least partly required for chemerin-mediated ***-mediated erythrotoxicity was additionally mediated by RNS,caspase-3,and ***,Glu^(21)-Ser^(157) chemerin promoted reduction in the liquid-ordered phase of cell membranes in ***:The present study first discloses that human chemerin can induce eryptosis via Ca^(2+)-dependent mechanisms at concentrations noticeably exceeding circulating ***,chemerin-induced eryptosis can hardly contribute to eryptosis-mediated anemia in disease

关键词： Adipokine Cell death Chemerin oxidative stress Eryptosis

Multiple Roles of HIV-1 Capsid during the Virus Replication Cycle

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Virologica Sinica 2019年第2期34卷 119-134页

作者： Mariia novikova Yulan Zhang Eric o. Freed Ke peng Virus-Cell Interaction Section HIV Dynamics and Replication Program Center for Cancer Research National Cancer Institute Frederick MD 21702 USA State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

Human immunodeficiency virus-1 capsid(HIV-1 CA) is involved in different stages of the viral replication cycle. During virion assembly, CA drives the formation of the hexameric lattice in immature viral particles, while in mature virions CA monomers assemble in cone-shaped cores surrounding the viral RNA genome and associated proteins. In addition to its functions in late stages of the viral replication cycle, CA plays key roles in a number of processes during early phases of HIV-1 infection including trafficking, uncoating, recognition by host cellular proteins and nuclear import of the viral preintegration complex. As a result of efficient cooperation of CA with other viral and cellular proteins, integration of the viral genetic material into the host genome, which is an essential step for productive viral infection, successfully occurs. In this review, we will summarize available data on CA functions in HIV-1 replication, describing in detail its roles in late and early phases of the viral replication cycle.

关键词： Human immunodeficiency virus-1 (HIV-1) Capsid (CA) Assembly post entry Uncoating and nuclear import Inhibitor

The dual choice of geodetic horizontal reference systems for Ukraine

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Geodesy and Geodynamics 2023年第3期14卷 254-264页

作者： Elena novikova Alena palamar Rostyslav Lopunov Department of Geodesy State Higher Educational InstitutionKryvyi Rih National UniversityKryvyi RihUkraine

Almost all European countries use at least two horizontal reference systems, which allow them to minimize all risks when changing the coordinate system. The first system is used for the needs of the national survey in general, and also for technical applications in engineering surveys. A brief description of this system can be written by the formula: Geocentric Ellipsoid(GRS80) + projection(UTM). More than half of the European countries for the second national systems use the GRS80 geocentric ellipsoid together with a projection other than UTM, some of them continue to use the projection of the old coordinate systems. The rest of the countries, excluding Ukraine, adapted the existing national coordinate systems to modern requirements, removing deformations from them concerning the ETRS89. A small number of European countries have implemented the GRS80 geocentric ellipsoid together with the UTM projection for cadastral surveys and topographic maps with a scale of 1:5000 and larger. only one country, Ukraine, chose a variant for the new coordinate system(UCS2000), which is briefly written by the formula: old Not Geocentric Ellipsoid + New projection. The analysis of modern coordinate systems of Europe made it possible to formulate two variants of the future coordinate system of Ukraine based on a geocentric ellipsoid. It is shown that the transition to a geocentric ellipsoid will change the coordinates and heights of the points, but practically will not change the distance between the points and the area of the parcels.

关键词： UTM GRS80 projection parameters Quantitative characteristics of land parcels UCS2000

A cozy niche in an iron world

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Signal Transduction and Targeted Therapy 2020年第1期5卷 297-298页

作者： Marcus Conrad Mariia novikova Helmholtz Zentrum München Institute of Metabolism and Cell DeathIngolstädter Landstr.185764 NeuherbergGermany pirogov Russian National Research Medical University Laboratory of Experimental OncologyOstrovityanova 1Moscow 117997Russia Federal Center of Brain Research and Neurotechnology Ostrovityanova1 bldg 10117997 MoscowRussia

In this study,Ubellacker et *** that unlike in blood vessels,the lymph is a more efficient route for tumor metastasis due to a more favorable redox environment,whereby disseminating cancer cells acquire additional pro... 详细信息

关键词： al metastasis saturated

New antioxidant SkQ1 is an effective protector of rat eye retinal pigment epithelium and choroid under conditions of long-term organotypic cultivation

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Advances in Aging Research 2012年第2期1卷 31-37页

作者： E. N. Grigoryan Y. p. novikova o. S. Gancharova o.V. Kilina p. p. philippov Belozersky Institute of physico-Chemical Biology Lomonosov Moscow State University Moscow Russia Institute of Mitoengineering Lomonosov Moscow State University Moscow Russia Kol’tsov Institute of Developmental Biology Russian Academy of Sciences Moscow Russia

Cells have intrinsic mechanisms for cleaning harmful oxidants represented mainly by reactive oxygen species (RoS). Despite the antioxidant defense, RoS can cause serious damage to the retina that with age leads to various eye diseases and even blindness. Among numerous cell sites of RoS generation, mitochondrial electron transport is of crucial importance. Recently, for the purpose of cleaning RoS in the mitochondrial matrix, powerful mitochondria- targeted antioxidant “SkQ1” has been invented. We studied SkQ1 effects upon tissues of rat posterior eye cup that consisted: retinal pigment epithelium (RpE) ? choroidal coat ? scleral coat. The eye cups were isolated from the eyes of adult albino rats and cultivated in rotary tissue culture system in the presence of 20 nM SkQ1 or without this compound. After 7 days - 1 month in vitro eye cup samples were studied by immunohistochemistry, routine histology, morphometry, and digital image analysis. We have found that under chosen, “in vitro like in vivo” conditions 20 nM SkQ1 effectively reduced cell death in RpE and choroid, protected RpE from disintegration caused by cell phenotypic transformation and withdrawal from the layer, suppressed transmigration of choroidal coat cells. In the ex vivo model we used degenerative processes were more pronounced in the eye cup center where SkQ1 effect was most vivid. All this give us hopes for effectiveness of SkQ1 treatment of retinal central part that is very susceptible to light-induced over-oxidation injury and mostly suffering in many age-related diseases, AMD, in particular.

关键词： Rat Eye Retinal pigment Epithelium Choroid organotypic Culturing in Vitro AMD Remodeling Antioxidant SkQ1 Cell Behavior Cell Death

New antioxidant SkQ1 is an effective protector of rat neural retina under conditions of long-term organotypic cultivation

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Advances in Aging Research 2013年第2期2卷 65-71页

作者： E. N. Grigoryan Y. p. novikova o. V. Kilina p. p. philippov Belozersky Institute of physico-Chemical Biology Lomonosov Moscow State University Moscow Russia Institute of Mitoengineering Lomonosov Moscow State University Moscow Russia Kol’tsov Institute of Developmental Biology Russian Academy of Sciences Moscow Russia Belozersky Institute of physico-Chemical Biology Lomonosov Moscow State University Moscow Russia Kol’tsov Institute of Developmental Biology Russian Academy of Sciences Moscow Russia Belozersky Institute of physico-Chemical Biology Lomonosov Moscow State University Moscow Russia

During life human eye is constantly exposed to sunlight and artificial light, the sources of reactive oxygen species (RoS)—the main cause of age-related eye pathology. A novel mitochondria-targeted antioxidant SkQ1 has recently been invented to reduce mitochondrial RoS by cleaning the mitochondria matrix, “the dirtiest place in the cell” in respect of RoS production and accumulation. Earlier we studied SkQ1 effects upon retinal pigment epithelium and choroid in the rat eye posterior cups exposed to long-term 3D organotypic culturing. It was found that under in vitro conditions 20 nM SkQ1 effectively reduced cell death in retinal pigment epithelium and choroid and protected the tissues from disintegration and cell withdrawal. In the present study we used same ex vivo conditions to examine the effect of SkQ1 upon the rat neural retina kept in the content of the posterior eye cup. Eye cups were isolated and cultured in vitro during 7, 14, and 30 days under rotation in the presence and absence of 20 nM SkQ1 in the culture medium. Serial sections of cultivated eye cups were subjected to histology, computer morphometry and immunohistochemistry. obtained results show that SkQ1 operates as a strong protective agent, preventing neuronal cell death and other degenerative processes in the neural retina. Cell rescue by SkQ1 was more vivid in the central part of the retina than at the periphery. That, in turn, suggests SkQ1 effectiveness in treatment of some age-related eye diseases when central part of the retina, including macula, is most susceptible to degeneration.

关键词： Rat Eye Neural Retina organotypic Culturing in Vitro Age-Related ophthalmic Disorders Antioxidant SkQ1 Cell-Type Specific proteins Cell Death

A New Approach to Determining the Time of Neotectonic Activity in Areas of Sedimentary Basins of Central Asia

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地学前缘 2009年第S1期16卷 196-196页

作者： Sophia novikova Institute of Geology and Mineralogy(Siberian Branch of the Russian Academy of Sciences)

Coal basins are common in sedimentary sequences of Central *** the Cenozoic activization their margins were intensely *** it is often impossible to define the deformation times via isotope geochronology because the latest orogeny was seldom accompanied by igneous *** the same time the deformation of sedimentary strata exposed coal beds with their afterwards *** coal fire temperatures(900-1300℃)were high enough

关键词： Cenozoic orogeny combustion meta-morphism coal fire 40Ar/39Ar dating K/Ar dating

Budget transparency and economic efficiency evaluation of innovative projects

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Budget transparency and economic efficiency evaluation of in...

Huge metastable axial strain in ultrathin heteroepitaxial vertically aligned nanowires

作者： T.S.novikova NSTU 630092 NovosibirskK.Marks20Russia

During transition to an innovative way of development there is a need for public efficiency analysis as a major component of investment projects evaluation. The budget transparency, based on clear presentation of a funds distribution criteria and corresponding to essential public participation, is investigated by using a model of intergovernmental flows. For aimed investment grants the budget transparency connected with the presentation of difference between financial and economic efficiency as a basis of governmental participation necessity for every investment project. A system of governmental support instruments was investigated on examples of two innovative projects: megaproject of new creking and reforming catalysts production and microproject of ecological housing construction in Novosibirsk region.

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Nano Research 2015年第6期8卷 1964-1974页

作者： Vivien Schuler Francisco Javier Bonilia Dominique Demaille Alessandro Coati Alina Vlad Yves Garreau Michele Sauvage-Simkin Anastasiia novikova Emiliano Fonda Sarah Hidki Victor Etgens Franck Vidal Yunlin Zheng Sorbonne Universites UpMC Institut des NonoSciences de Paris 4 place Jussieu Paris 75005 France CNRS UMR7588 Institut des NanoSciences de Paris 4 place Jussieu Paris 75005 France Synchrotron Soleil L'Orme des Merisiers Saint-Aubin BP 48 91192 Gif-sur-Yvette Cedex France Universite paris Diderot Sorbonne Paris Cite MPO UMR 7162 CNRS Batiment Condorcet Case courrier 7021 75205 Paris Cedex 13 France Universite de Versailles Saint-Quentin en Yvelines 55 Av. de Paris Versailles 78035 France

Strain engineering is a powerful tool to tailor the physical properties of materials coherently stacked in an epitaxial heterostructure. Such an approach, applied to the mature field of planar heteroepitaxy, has yielded a variety of new phenomena and devices. Recently, heteroepitaxial vertically aligned nanocomposites have emerged as alternatives to planar structures. owing to the peculiar geometry of such nanoarchitectures, efficient strain control can be achieved, opening the way to novel functionalities. In this paper, we report a very large tensile axial strain in epitaxial transition metal nanowires embedded in an oxide matrix. We show that axial strains in excess of 1.5% can be sustained over a large thickness （a few hundred nanometers） in epitaxial nanowires having ultrasmall diameters （-3-6 nm）. The axial strain depends on the diameter of the nanowires, reflecting its epitaxial nature and the balance of interface and elastic energies. Furthermore, it is experimentally shown that such strain is metastable, in agreement with the calculations performed in the framework of the Frenkel-Kontorova model. The diameter dependence and metastability provide effective ways to control the strain, an appealing feature for the design of functional nanoarchitectures.

关键词： self-assembly heteroepitaxy nanowires strain