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iLIVER 2023年第3期2卷 170-176页

作者： Eduarda Raisa Coser Raphael Bernardo Neto Izabel Cristina Meister Martins Coelho norma possa marroni Claudio Augusto marroni Camila Moraes Marques Faculdades Pequeno Principe Brazil Universidade Federal de Ciencias da Saude de Porto Alegre Brazil

The texts analyzed in full totaled 110,of which 36 articles published between 2001 and 2020 were selected,with quantitative approaches,case reviews,liter-ature reviews,meta-analyses,and *** is concluded that beta-blockers are the drugs of choice for primary prophylaxis of variceal *** for secondary prophylaxis,there is an association of these with nitrates and endoscopic *** regard to acute bleeding,the use of vasoactive drugs is chosen,especially ***,it is important to emphasize that there is a shortage of clinical studies and a need for investment in new therapies.

关键词： Portal hypertension Clinical treatment pharmacology and variceal bleeding

Glutamine prevents oxidative stress in a model of mesenteric ischemia and reperfusion

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World Journal of Gastroenterology 2014年第32期20卷 11406-11414页

作者： Gilmara Pandolfo Zabot Gustavo Franco Carvalhal norma possa marroni Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann Department of Surgery School of Medicine Pontifical Catholic University of Rio Grande do Sul Porto Alegre RS 90610-000 Brazil Department of Gastroenterology School of Medicine Federal University of Rio Grande do Sul Porto Alegre RS 90035-903 Brazil Department of Pathology School of Medicine Pontifical Catholic University of Rio Grande do SulPorto Alegre RS 90610-000 Brazil

AIM: To evaluate preventative effects of glutamine in an animal model of gut ischemia/reperfusion (I/R).

关键词： Ischemia-reperfusion Glutamine Lipid peroxidation Superoxide dismutase Nuclear factor-kappa beta Interleukin 6

Glutamine prevents oxidative stress in a model of portal hypertension

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World Journal of Gastroenterology 2017年第25期23卷 4529-4537页

作者： Gilmara Pandolfo Zabot Gustavo Franco Carvalhal norma possa marroni Francielli Licks Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann Department of Surgery School of MedicinePontifical Catholic University of Rio Grande do Sul Department of Gastroenterology School of MedicineFederal University of Rio Grande do Sul Department of Pathology School of MedicinePontifical Catholic University of Rio Grande do Sul

To evaluate the protective effects of glutamine in a model of portal hypertension (PH) induced by partial portal vein ligation (PPVL). METHODSMale Wistar rats were housed in a controlled environment and were allowed access to food and water ad libitum. Twenty-four male Wistar rats were divided into four experimental groups: (1) control group (SO) - rats underwent exploratory laparotomy; (2) control + glutamine group (SO + G) - rats were subjected to laparotomy and were treated intraperitoneally with glutamine; (3) portal hypertension group (PPVL) - rats were subjected to PPVL; and (4) PPVL + glutamine group (PPVL + G) - rats were treated intraperitoneally with glutamine for seven days. Local injuries were determined by evaluating intestinal segments for oxidative stress using lipid peroxidation and the activities of glutathione peroxidase (GPx), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) after PPVL. RESULTSLipid peroxidation of the membrane was increased in the animals subjected to PH (P 0.05). The activity of the antioxidant enzyme GTx was decreased in the gut of animals subjected to PH compared with that in the control group of animals not subjected to PH (P 0.05). At least 10 random, non-overlapping images of each histological slide with 200 × magnification (44 pixel = 1 μm) were captured. The sum means of all areas, of each group were calculated. The mean areas of eNOS staining for both of the control groups were similar. The PPVL group showed the largest area of staining for eNOS. The PPVL + G group had the second highest amount of staining, but the mean value was much lower than that of the P

关键词： Portal hypertension Endothelial nitric oxide synthase Glutamine Glutathione peroxidase Inducible nitric oxide synthase Lipid peroxidation

Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation

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World Journal of Gastroenterology 2016年第40期22卷 8918-8928页

作者： Josieli Raskopf Colares Elizângela Goncalves Schemitt Renata Minuzzo Hartmann Francielli Licks Mariana do Couto Soares Adriane Dal Bosco norma possa marroni Graduate Program in Medical Sciences Universidade Federal do Rio Grande do Sul Porto Alegre 90040-060 Brazil Laboratory of Experimental Hepatology and Gastroenterology Hospital de Clínicas de Porto Alegre Porto Alegre 90035-903 Brazil Laboratory of Oxidative Stress and Antioxidants Universidade Luterana do Brasil Canoas 92425-900 Brazil Graduate Program in Physiology Universidade Federal do Rio Grande do Sul Porto Alegre 90050-170 Brazil

AIM To evaluate the effects of melatonin(Mel) on oxidative stress in an experimental model of bile duct ligation(BDL).METHODS Male Wistar rats(n = 32, weight ± 300 g) were allocated across four groups: CO(sham BDL), BDL(BDL surgery), CO + Mel(sham BDL and Mel administration) and BDL + Mel(BDL surgery and Mel administration). Mel was administered intraperitoneally for 2 wk, starting on postoperative day 15, at a dose of 20 mg/*** Mel was effective at the different standards, reestablishing normal liver enzyme levels, reducing the hepatosomatic and splenosomatic indices, restoring lipoperoxidation and antioxidant enzyme concentrations, reducing fibrosis and inflammation, and thereby reducing liver tissue injury in the treated *** The results of this study suggest a protective effect of Mel when administered to rats with secondary biliary cirrhosis induced by BDL.

关键词： Antioxidant Cirrhosis Fibrosis Melatonin Oxidative stress

Antioxidant properties of glutamine and its role in VEGF-Akt pathways in portal hypertension gastropathy

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World Journal of Gastroenterology 2013年第28期19卷 4464-4474页

作者： Camila Marques Francielli Licks Ingrid Zattoni Beatriz Borges Luiz Eduardo Rizzo de Souza Claudio Augusto marroni norma possa marroni Universidade Federal do Rio Grande do Sul-PPG Fisiologia Porto Alegre 90000100 Brazil Universidade Federal do Paraná Curitiba 81531900 Brazil Departamento de Medicina Interna-Gastroenterologia-Universidade Federal de Ciências da Saúde de Porto Alegre Porto Alegre 90000100 Brazil Universidade Federal do Rio Grande do Sul-PPG Fisiologia Universidade Luterana do Brasil Porto Alegre 90000100 Brazil

AIM: To investigate the effects of glutamine on oxidative/nitrosative stress and the vascular endothelial growth factor (VEGF)-Akt-endothelial nitric oxide synthase (eNOS) signaling pathway in an experimental model of portal hypertension induced by partial portal vein ligation (PPVL). METHODS: Portal hypertension was induced by PPVL. The PPVL model consists of a partial obstruction of the portal vein, performed using a 20 G blunt needle as a guide, which is gently removed after the procedure. PPVL model was performed for 14 d beginning treatment with glutamine on the seventh day. On the fifteenth day, the mesenteric vein pressure was checked and the stomach was removed to test immunoreactivity and oxidative stress markers. We evaluated the expression and the immunoreactivity of proteins involved in the VEGF-Akt-eNOS pathway by Western blotting and immunohistochemical analysis. Oxidative stress was measured by quantification of the cytosolic concentration of thiobarbituric acid reactive substances (TBARS) as well as the levels of total glutathione (GSH), superoxide dismutase (SOD) activity, nitric oxide (NO) production and nitrotyrosine immunoreactivity. RESULTS: All data are presented as the mean ± SE. The production of TBARS and NO was significantly increased in PPVL animals. A reduction of SOD activity was detected in PPVL + G group. In the immunohistochemical analyses of nitrotyrosine, Akt and eNOS, the PPVL group exhibited significant increases, whereas decreases were observed in the PPVL + G group, but no difference in VEGF was detected between these groups. Western blotting analysis detected increased expression of phosphatidylinositol-3-kinase (PI3K), P-Akt and eNOS in the PPVL group compared with the PPVL + G group, which was not observed for the expression of VEGF when comparing these groups. Glutamine administration markedly alleviated oxidative/nitrosative stress, normalized SOD activity, increased levels of total GSH and blocked NO overproduction as well

关键词： Partial portal vein ligation Oxidative stress Glutamine Portal hypertension Rats

N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats

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World Journal of Gastroenterology 2015年第43期21卷 12351-12360页

作者： Francielli Licks Renata Minuzzo Hartmann Camila Marques Elizangela Schemitt Josieli Raskopf Colares Mariana do Couto Soares Juliana Reys Camila Fisher Juliana da Silva norma possa marroni Universidade Federal do Rio Grande do Sul Instituto de Tecnologia do Paraná Universidade Luterana do Brasil

AIM: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal ***: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet ***: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P < 0.001). The same was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P < 0.001),VEGF(34.9 ± 4.7 vs 17.46 ± 2.6 pixels)(P < 0.05),and NTT(39.01 ± 4.0 vs 12.77 ± 2.3 pixels)(P < 0.05) expression by immunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P < 0.01) and VEGF(0.38 ± 0.04 vs 0.26 ± 0.04 a.μ)(P < 0.01) were also evaluated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P < 0.001) when compared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P < 0.001

关键词： N-Acetylcysteine Portal hypertension Gastropathy Oxidative stress Antioxidant