N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats

作     者：Francielli Licks Renata Minuzzo Hartmann Camila Marques Elizangela Schemitt Josieli Raskopf Colares Mariana do Couto Soares Juliana Reys Camila Fisher Juliana da Silva Norma Possa Marroni 

作者机构：Universidade Federal do Rio Grande do Sul Instituto de Tecnologia do Paraná Universidade Luterana do Brasil 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第43期

页      面：12351-12360页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Brazilian agencies Coordena??o de Aperfei?oamento de Pessoal de Nível Superior,Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundo de IncentivoàPesquisa e Eventos-Hospital de Clínicas of Porto Alegre(FIPE project no11-0293) the Laboratory of Experimental Hepatology and Gastroenterology(HCPA/UFRGS) Universidade Federal doRio Grande do Sul the Oxidative Stress and Antioxidants Laboratory Universidade Luterana do Brasil 

主　　题：N-Acetylcysteine Portal hypertension Gastropathy Oxidative stress Antioxidant 

摘      要：AIM: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal ***: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet ***: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P 0.001). The same was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P 0.001),VEGF(34.9 ± 4.7 vs 17.46 ± 2.6 pixels)(P 0.05),and NTT(39.01 ± 4.0 vs 12.77 ± 2.3 pixels)(P 0.05) expression by immunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P 0.01) and VEGF(0.38 ± 0.04 vs 0.26 ± 0.04 a.μ)(P 0.01) were also evaluated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P 0.001) when compared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P 0.001