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Determination of aerosol extinction coefficient and mass extinction efficiency by DOAS with a flashlight source

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Chinese Physics B 2005年第11期14卷 2360-2364页

作者：司福祺刘建国谢品华张玉钧刘文清 Hiroaki Kuze 刘诚 Nofel Lagrosas nobuo Takeuchi Key Laboratory of Environmental Optics & Technology Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciencess Hefei 230031 China Center for Environmental Remote Sensing Chiba University Chiba 263-8522 Japan

With the method of differential optical absorption spectroscopy （DOAS）, average concentrations of aerosol particles along light path were measured with a flashlight source in Chiba area during the period of one month. The optical thickness at 550 nm is compared with the concentration of ground-measured suspended particulate matter （SPM）. Good correlations are found between the DOAS and SPM data, leading to the determination of the aerosol mass extinction efficiency （MEE） to be possible in the lower troposphere. The average MEE value is about 7.6m^2.g^-1 , and the parameter exhibits a good correlation with the particle size as determined from the wavelength dependence of the DOAS signal intensity.

关键词： aerosol extinction coefficient differential absorption optical spectroscopy mass extinction efficiency size distribution

用光子回声测量LaF3:Pr3+的弛豫时间

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发光学报 1975年第6期 33-34页

作者： nobuo Takeuchi 日本国立环境研究所

本文报导首次用光子回声法测量稀土离子的非克喇末(non-Kramer's)盐在液氮温度下的弛豫时间。光子回声用高重复性可调染料激光器激发,籍助于数据平均法可作精确的测量。

关键词：弛豫时间 LaF3 回声反射声染料激光器液体激光器光子玻色子

Plant Digestive Supplement Designed by Lactobacillus Regulated Leukocyte Subsets through Activation of Complement Components and Implication for Use against Tumor Bearing Host against Infection

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Open Journal of Immunology 2015年第3期5卷 133-146页

作者： Kohji Ohtubo nobuo Yamaguchi Nurmuhamamt Amat Dilxat Yimit Parida Hoxur Hiroshi Ushijima Yousuke Watanabe Ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Fundament Research for CAM Kanazawa Medical University Ishikawa Japan Traditional Uighur Medicine Department Xinjiang Medical University Urumqi China Traditional Chinese Medicine Hospital Xinjiang Medical University Urumqi China

A plant material consisted by Family Poaceae was fermented by Yeast and Lactobaccilli (U-164). This material was proved by as safe in animal safety experiment for oral administration. In order to prove the effect of U-164 against physiological function, the animal and human trials were set up to look into mainly leukocyte functions. In animal experiment, anti-oxidative effect and antibody response in immune-compromised host and diabetes meritus were made up. For human use, peripheral lymphocyte in number and subset ratio were followed up to one month after administration. In order to understand its effect, human complement component analysis was made by immune-electrophoresis. Our results showed that U-164 augmented the level of lymphocytes, while U-164 down regulated the level of granulocytes. In our clinical study with 19 healthy volunteers, granulocyte and lymphocyte ratio was obtained as neutral in peripheral blood being increased significantly 30 days after the ingestion of U-164. In experimental animal study, the compromised host as well as normal animal was administered with cancer chemotherapeutic agent (Mytomycin-C). Our observations showed against antibody producing cell, this material recovered the antibody production in the host compromising the immure responsiveness. We also proposed an idea that U-164 exhibited tonic effects via activating complement components. Moreover, we tried to access further to the anti-oxidative activities of this U-164. This modification brought to the significant lifted up for anti-oxidative activity for phagocytic cell.

关键词： Family Poaceae Fermentation Yeast Lactobacillus Cancer Chemotherapeutic Agent Compromised Host Complement C3b Fragment Anti-Oxidant Diabetes Blood Sugar

Quantitative &Qualitative Regulation of Peripheral Leukocyte Subsets plus Emotional Hormones by Floor Heating

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Open Journal of Rheumatology and Autoimmune Diseases 2014年第2期4卷 97-105页

作者： nobuo Yamaguchi Amat Nurmuhammad Jameas Zhao Xiufeng Jia Jiao Jian Rui Chen Tsugiya Murayama Deaprtment of Microbiology and Immunology Faculty of Pharmaceutical Sciences Hokuriku University Kanazawa Japan Ishikawa Natural Medicinal Products Research Center Kanazawa Japan Uighur Medicine Department of Xinjiang Medical University Urumqi City China Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

A floor heating system is a traditionary famous in Asian countries, especially in North East of China and Korea. In this report, we intended to clarify the effect of floor hating system for accessing a peripheral leukocyte regulation in winter season by evidence-based manner more than VAS. We set up two different office rooms heat-controlled by floor heating and air-conditioned by ceiling electric heater. Both qualitative and quantitative approaches determine which are better therapeutic effective recipes for this trial. The elements were evaluated by the total number of peripheral leukocyte, granulocyte and lymphocyte ratio and lymphocyte subsets. For 5 days of working in each conditioned room, the volunteers were changed the room after one-week interval for cooling down. Floor heating system regulated total number of peripheral leukocyte. The mode of the action for the high number of the volunteer was down-regulated. On the contrary, in lower numbers it was up-regulated after the trial. The number of leukocyte subsets, granulocyte and lymphocyte were also regulated by dose dependent manner. Emotional hormone, adrenalin, cortisone and nor-adrenaline were down regulated by floor heating but dopamine was up-regulated by this heating system. The reversal results were obtained by the group who worked in the conditioned room by the system heating from ceiling.

关键词： Room Temp Control Floor Heating Leukocyte Subsets Ratio Emotional Hormone

Overall Estimation of Anti-Oxidant Activity by Mammal Macrophage

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Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 13-21页

作者： nobuo Yamaguchi Natsuko Kawada Xinfeng Jia Kazuhiro Okamoto Katsuko Okuzumi Rui Chen Takashi Takahashi Department of Fundamental Research for CAM Kanazawa Medical University Ishikawa Japan Ishikawa Natural Medicinal Products Research Center Kanazawa Japan Kanazawa University Graduate School of Medical Science Kanazawa Japan Laboratory of Infectious Diseases Graduate School of Infection Control Sciences Kitasato University Tokyo Japan. Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Super oxide anion had been known as a critical role in a phagocytic cell when microorganisms invade inside. However, this radical seldom need for a clean environment such in a developed country. Moreover, this agent sometimes triggered as serious factor in the site of inflammation. There are many papers which concerned newly found anti-oxidative substances. However, many reports only focused on the molecular basis in vitro, how oxidative stress damaged the human tissue. The key factor for oxidative stress needs to be discussed first for immunological standpoint, such as armed reduction treaty. In other words, minimum armed force is necessary for an invasion by outer or inner irregular cells. On the basis of phagocytic function of the cell in vivo, we tried to access anti-oxidative activity by employing peritoneal murine macrophage and human monocyte. So, the purpose of this study is to establish a conventional but tailor made system for which is useful for evaluating the anti-oxidative activity to different constition. Especially in human peripheral monocyte, the sample was tested each constitution such in granulocyte rich type and lymphocyte rich typ. In order to establish the direct estimation by phagocytic cell, a murine peritoneal exudates cell suspension was obtained after one week of oral administration with anti-oxidative sample. The samples we selected were traditional Chinese medicine, Shi-Quan-Da-Bu-Tang (SDT), Bu-Zong-Ye-Qi-Tang (BYT) and Shao-Chin-Rong-Tang sCRT), Agaricus, Chlorella, and Propolis. The amount of generated super oxide anion was calculated through measuring the optical density change of this supernatant using cytochrome-C which was reduced by generated super oxide anion in the phagocytic cell. The generated super oxide anion after administration of Agaricus, Chlorella, SDT, BYT, sCRT and Propolis were 2.64, 1.95, 1.24, 1.25, 2.88, 2.55 × 10﹣5 mmol/ml, respectively, whereas 2.85 × 10﹣5 mmol/ml in control group. The fermented sample from SDT

关键词： Anti-Oxidant Sample Super Oxide Anion Neutrophil Macrophage Cytochrome-c Constitution

体内冷冻的正常及镉处理小鼠睾丸内血清蛋白质的免疫组织化学研究

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生殖与避孕 2006年第3期26卷 135-141页

作者：廖晓岗 nobuo TERADA 李子龙 Nobuhiko OHNO Yasuhisa FUJII Shinichi OHNO 重庆医科大学电子显微镜室重庆400016 山梨大学医学工学综合研究部解剖学系中国医科大学第一附属医院沈阳110001

目的:了解正常和镉(Cd)处理小鼠睾丸内血清蛋白质渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法:保持正常动力学的血循环下,运用活体内冷冻技术对曲细精管内白蛋白和IgG进行快速冷冻固定。冷冻固定的睾丸行冷冻置换后作... 详细信息

目的:了解正常和镉(Cd)处理小鼠睾丸内血清蛋白质渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法:保持正常动力学的血循环下,运用活体内冷冻技术对曲细精管内白蛋白和IgG进行快速冷冻固定。冷冻固定的睾丸行冷冻置换后作石蜡包埋。5μm厚连续切片分别用于抗鼠血清白蛋白和IgG的免疫组化染色和H.E.染色。结果:正常曲细精管,白蛋白免疫反应产物主要定位于管周肌样细胞周围,以及间质细胞之间和毛细血管内,部分免疫反应产物呈拱型出现在曲细精管上皮基底室内,沿精原细胞表面分布。在依据生精细胞排列而区分的曲细精管上皮周期的不同发育时相,拱型免疫反应产物的数量不同。正常小鼠睾丸内IgG免疫反应的定位型式与白蛋白的免疫定位相似。Cd处理后24h,部分曲细精管上皮内空泡形成和管腔增大,白蛋白和IgG免疫反应产物不仅出现在基底室,而且也见于近腔室的支持细胞和生精细胞之间。结论:运用活体内快速冷冻技术结合血清白蛋白和IgG的免疫组化方法,能清楚地揭示正常和Cd处理小鼠曲细精管内血清蛋白质的不同免疫染色型式,反映体内支持细胞间紧密连接结构的变化。

关键词：免疫组化活体内冷冻技术血清蛋白质睾丸镉(Cd)

Comparative studies of serum-free media and detection techniques for in vitrodrug sensitivity assessment of Plasmodium falciparum

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Open Journal of Clinical Diagnostics 2013年第3期3卷 115-121页

作者： Bethel Kwansa-Bentum Shinji Izumiyama Kei Kitamura Kazushige Obata-Ninomiya nobuo Ohta Hiroko Asahi Department of Animal Biology and Conservation Science Faculty of Science University of Ghana Accra Ghana Department of Environmental Parasitology Tokyo Medical and Dental University Tokyo Japan Department of Immune Regulation Tokyo Medical and Dental University Tokyo Japan Department of Parasitology National Institute of Infectious Diseases Tokyo Japan

Malaria continues to be a devastating disease. In a previous study, we formulated a chemically defined culture medium that is able to sustain the complete intraerythrocytic growth of Plasmodium falciparum. We tested the feasibility of using the medium (CDRPMI) as well as human serum-free media enriched with commercially available human-serum substitutes (GFSRPMI and ALBRPMI) to assess the drug sensitivity of P. falciparum, using chloroquine diphosphate (CQ) and dihydroartemisinin (DHART) as conventional antimalarial drugs. Growth inhibition was measured by four different methods: flow cytometry with SYBR Green I (FCM), microscopy (Giemsa method), enzymatic estimation of parasite lactate dehydrogenase (pLDH), and histidine-rich protein 2 (HRPII) determination. In drug sensitivity tests on asynchronous parasites cultured for 96 h in the presence of drugs, the dose-response curves were similar and differences in the 50% growth inhibition concentrations for the drugs, which were estimated by the four methods, were not statistically significant for the three culture media. The effect of the drugs on the growth of synchronous parasites at the ring stage was also assessed in micro-volume tests by three different methods of FCM: tracking fluorescent erythrocytes, schizont test, and merozoite test. Dose-response curves for the drugs were similar, and differences in the 50% growth inhibition concentrations were not statistically significant for CDRPMI and GFSRPMI. Thus CDRPMI as well as GFSRPMI and ALBRPMI can be similarly useful media for drug sensitivity testing of P. falciparum. The FCM, pLDH and HRPII estimations were fast and reliable detection methods, with FCM allowing schizont and merozoite tests to be performed with shorter periods of culture.

关键词： Plasmodium falciparum Chemically Defined Medium Drug Sensitivity Test Flow Cytometry

LactobacilliEnjoyed Fermented Herbs on to the Last Fragment and Regulated Leucocyte Subsets and Anti-Oxidative Activity

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Open Journal of Rheumatology and Autoimmune Diseases 2017年第1期7卷 30-45页

作者： nobuo Yamaguchi Yoshiichiroh Mastuba Kazuhiro Okamoto Daisuke Sakamoto Takanao Ueyama Hideo Matsuno Nurmuhamamt Amat Ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Rehabilitation Kanazawa Medical University Uchinada-Machi Japan Department of Heart Surgery Kanazawa Medical University Himi Municipal Hospital Himi Japan Department of 2nd Internal Medicine Kansai Medical University Osaka Japan Department of Otolaryngology Kanazawa Medical University Uchinada-Machi Japan Traditional Uighur Medicine Department Xinjiang Medical University Urumqi China

Eighty sorts of herbs were fermented by Lactobaccilli (f-ESH). This material was proved by as safe in animal safety experiment. This work was aimed to revise the traditional way of hot water extraction to the fermentation, in order to use up the original material and searching new activity as well. We tried to prove the new activity by fermentation for immune-competent cells in the host that administrated both of original remedy and the new fermented sample. In rodents, immune-compromised host was prepared with cancer chemotherapeutic agent. Our observations showed that the antigen stimulated animal increased the antibody producing cell for heterologous erythrocyte by compromised host by f-ESH. Moreover, by administration of f-ESH to immno-suppressed hosts, these samples regulated lymphocyte in number and functions, macrophage activities, and regulating anti-oxidative activity by phagocytic cells. The anti-oxidative assay was carried out ex-vivo system by peritoneal excaudate cell that we propose as suitable system for evaluating antioxidative assay. With these evidences, the original COF only augmented the level of lymphocytes in number, while f-ESH regulated the ratio of granulocytes and lymphocyte. In clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio was also resulted as neutral in peripheral white blood cells, 1, 2 and 3 weeks after the administration of f-ESH. We discussed the significance and mechanism of cleving complement components Fb by f-ESH in this text. Fb. The new cleavage of complement was directly evident by immune-electrophoretic method. In conclusion, this modification of food materials introduced new style of food intake resulted in the significant regulation such as antibody producing cells and anti-oxidative activity for phagocytic cells comparing traditional processing by hot water extraction.

关键词： Wild Herbs Fermentation Lactobacilli Lymphocyte Granulocyte Ant-Oxidative Activity GABA Complement Alternative Complement Activation

Overexpression of thioredoxin in transgenic mice reduces neuronal degeneration after mild focal ischemia

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Overexpression of thioredoxin in transgenic mice reduces neu...