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Plasmonic nanoparticles and nucleic acids hybrids for targeted gene delivery,bioimaging,and molecular recognition

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Journal of Innovative Optical Health Sciences 2021年第4期4卷 3-30页

作者： Timofey Pylaev Elena Avdeeva nikolai khlebtsov Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences Saratov 410049Russia Razumovsky Saratov State Medical University Saratov 410012Russia Federal Center of Agriculture Research of the South-East Region Saratov 410010Russia Saratov National Research State University Saratov 410012Russia

Promising biomedical applications of hybrid materials composed of gold nanoparticles and nucleic acids have attracted strong interest from the nanobiotechnological *** particular interest is owing to the robust and easy-to-make synthetic approaches,to the versatile optical and catalytic properties of gold nanoparticles combined with the molecular recognition and programmable properties of nucleic *** significant progress is made in the develop-ment of DNA-gold nanostructures and their applications,such as molecular recognition,cell and tissue bioimaging,targeted delivery of therapeutic agents,*** review is focused on the critical discussion of the recent applications of the gold nanoparticles-nucleic acids *** effect of particle size,surface,charge and thermal properties on the interactions with functional nucleic acids is *** each of the above topics,the basic principles,recent advances,and current challenges are *** is placed on the systematization of data over the theranostic systems on the basis of the gold nanoparticles-nucleic acids ***,we start our discussion with observation of the recent data on interaction of various gold nano-particles with nucleic *** we describe existing gene delivery systems,nucleic acids detection,and bioimaging ***,we describe the phenomenon of the polymerase chain reaction improvement by gold nanoparticle additives and its potential underlying ***,we provide a short summary of reported data and outline the challenges and perspectives.

关键词： gold nanoparticles delivery DNA detection bioimaging

Introduction to the special issue on surface-enhanced Raman spectroscopy and functionalized plasmonic nanoparticles for biomedical applications

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Journal of Innovative Optical Health Sciences 2021年第4期4卷 1-2页

作者： Jian Ye Haifeng Yang nikolai khlebtsov School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030P.R.China College of Chemistry and Materials Science Shanghai Normal University Shanghai 200234P.R.China Laboratory of Nanobiotechnology Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences(IBPPM RAS) Saratov 410049Russia

Since its first discovery in 1974 from the enhanced Raman spectra of pyridine molecules on roughened silver surface,surface-enhanced Raman spectrosco-py(SERS)has garnered significant attention in the field of chemistry,biology,and *** the sensitivity of down to single-molecule level and the intrinsic"fingerprint"spectrum,SERS enables the ultra-sensitive,specific,selective,and multi-plexing label-free analysis of a trace of molecules in aqueous biological environments,minus the inter-ference from water,white-light or tissue auto-fluorescence background.

关键词： surface silver SERS

SERS-based lateral flow immunoassay of troponin I by using gapenhanced Raman tags

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Nano Research 2019年第2期12卷 413-420页

作者： Boris N. khlebtsov Daniil N. Bratashov Nadezhda A. Byzova Boris B. Dzantiev nikolai G. khlebtsov Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Academy of Sciences Saratov 410049 Russia Saratov State University Saratov 410012 Russia A. N. Bach Institute of Biochemistry Research Centre of Biotechnology Russian Academy of Sciences Moscow 119071 Russia

The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.

关键词： surface-enhanced Raman scattering (SERS) lateral flow immunoassay Au core/shell nanorods gap-enhanced Raman tags cardiac troponin I

Gold nanorods with a hematoporphyrin-loaded silica shell for dual-modality photodynamic and photothermal treatment of tumors in vivo

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Nano Research 2014年第3期7卷 325-337页

作者： Georgy Terentyuk Elizaveta Panfilova Vitaly Khanadeev Daniil Chumakov Elina Genina Alexey Bashkatov Valery Tuchin Alia Bucharskaya Galina Maslyakova nikolai khlebtsov Boris khlebtsov Saratov State University 83 Astrakhanskaya Saratov 410012 Russia Institute of Biochemistry and Physiology of Plants and Microorganisms 13 pr. Entuziastov Saratov 410049 Russia Institute of Precise Mechanics and Control of RAS 24 Rabochaya Saratov 410028 Russia Ulyanovsk State University 42 Lev Tolstoy Ulyanovsk 432017 Russia Optoelectronics andMeasurement Techniques Laboratory UniversityofOulu P.O. Box4500 Oulu FIN-90014 Finland Saratov State Medical University 112 Bolshaya Kazachia Saratov 410012 Russia

Nanocomposites （NCs） consisting of a gold nanorod core and a mesoporous silica shell doped with hematoporphyrin （HP） have been fabricated in order to improve the efficiency of cancer treatment by combining photothermal and photodynamic therapies （PDT ＋ PTT） in vivo. In addition to the long-wavelength plasmon resonance near 810-830 nm, the fabricated NCs exhibited a 400-nm absorbance peak corresponding to bound HP, generated singlet oxygen under 633-nm excitation near the 632.5-nm Q-band, and produced heat under a 808-nm near-infrared （NIR） laser irradiation. These modalities were used for a combined PDT ＋ PTT treatment of large （about 3 cm3） solid tumors in vivo with a xenorafted tumor rat model. NCs were directly injected into tumors and irradiated simultaneously with 633-nm and 808-nm lasers to stimulate the combined photodynamic and photothermal activities of NCs. The efficiency of the combined therapy was evaluated by optical coherence tomography, histological analysis, and by measurements of the tumor volume growth during a 21-day period. The NC-mediated PDT led to weak changes in tissue histology and to a moderate 20% decrease in the tumor volume. In contrast, the combined PDT ＋ PTT treatment resulted in the large-area tumor necrosis and led to dramatic decrease in the tumor volume.

关键词： nanocomposites gold nanorods mesoporous silica hematoporphyrin photodynarnic andphotothermal therapy

The morphological changes in the internal organs of laboratory animals after prolonged oral administration of gold nanoparticles

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Journal of Innovative Optical Health Sciences 2016年第4期9卷 74-84页

作者： Alla B.Bucharskaya Galina N.Maslyakova Svetlana S.Pakhomy Olga V.Zlobina Irina O.Bugaeva Nikita A.Navolokin Boris N.khlebtsov Vladimir A.Bogatyrev nikolai G.khlebtsov Valery V.Tuchin Saratov State Medical University n.a.V.I.Razumovsky 410012 Saratov Russia Institute of Biochemistry and Physiology of Plants and Microorganisms RAS 410049 Saratov Russia Research-Educational Institute of Optics and Biophotonics Saratov National Research State University 410012 Saratov Russia Institute of Precision Mechanics and Control RAS 410028 Saratov Russia Interdisciplinary Laboratory of Biophotonics Tomsk National Research State University 634050 TomskRussia

Recently gold nanoparticles(GNPs)have been actively studied as photot hermal converters,drug carriers,and imaging agents in a wide range of applications in cancer diagnosis and ther *** prolonged peroral administration of GNPs in a range of sizes was performed to investigate the morphological changes and their reversibility in the internal organs of laboratory *** this study,GNP's with average diameters of 2 nm,15 nm and 50nm were administered during 30 days,and the reversibility of morphological changes was investigated 14 days after *** the prolonged administration of GNPs,the severity of morphological changes in the liver,kidney,spleen and lymph nodes depended on the nanoparticle ***,50 nm nano-particles caused the most pronounced dystrophic and necrobiotic effects,whereas the smallest 2 nm nanoparticles caused proliferative *** importantly,the development of patho-logical processes was reversible,as evidenced by the gradual restoration of the organ structure at 14 days after the end of GNPs administration.

关键词： Gold nanoparticles prolonged peroral administration morphological changes reversibility.

Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats

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Journal of Innovative Optical Health Sciences 2016年第1期9卷 47-56页

作者： Alla B.Bucharskaya Svetlana S.Pakhomy Olga V.Zlobina Galina N.Maslyakova Olga V.Matveeva Irina O.Bugaeva Nikita A.Navolokin Boris N.khlebtsov Vladimir A.Bogatyrev nikolai G.khlebtsov Valery V.Tuchin Saratov State Medical University 112 B.Kazach'ya str.Saratov 410012Russia Institute of Biochemistry and Physiology of Plants and Microorganisms RAS 13 Entuziastov Prospekt.Saratov 410049Russia Saratov State University 83 Astrakhanskaya str.Saratov 410012Russia§Institute of Precise Mechanics and Control of Russian Academy of Sciences 24Rabochaya str.Saratov 410028Russia Interdisciplinary Laboratory of Biophotonics Tomsk State University 36 Lenina str.Tomsk 634050Russia

At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and ***,the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant ***,we report the effects of prolonged peroral administration of GNPs with different sizes(2,15 and 50 nm)on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory *** experiment was conducted on 24 white mongrel male rats weighing 180–220 g,gold nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosage of 190g/kg of animal body *** GNPs were conjugated with polyethylene glycol to increase their biocompatibility and *** size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of GNPs with size of 50 *** stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone *** signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing number of immunoblasts and large *** quantitative changes of cellular component morphology of lymphoid organs due to activation of migration,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of GNPs.

关键词： Gold nanoparticles lymphoid organs morphological changes bone marrow examination