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SERS-based lateral flow immunoassay of troponin I by using.g.penhanced Raman tag.

Nano Research 2019年第2期12卷 413-420页

作者： Boris N. khlebtsov Daniil N. Bratashov Nadezhda A. Byzova Boris B. Dzantiev nikolai g. khlebtsov Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms Russian Academy of Sciences Saratov 410049 Russia Saratov State University Saratov 410012 Russia A. N. Bach Institute of Biochemistry Research Centre of Biotechnology Russian Academy of Sciences Moscow 119071 Russia

The lateral flow immuno assay (LFIA) has emerg.d as a powerful tool for rapid scree ning.owing.to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantag.s, including.limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng.LFIA based on non spherical g.p-e nhanced Raman tag. (g.RTs), with Raman molecules (RMs) embedded in a 1-nm g.p between Au nanorod core and Au shell. Such tag. have a strong.and uniform surface-enhanced Rama n scattering.(SERS) resp on se, an order of mag.nitude hig.er than that of other comm on SERS tag. such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical g.RTs with embedded RMs. The feasibility of the tag. was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). g.RTs were conjug.ted with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal g.ld. The presenee of the targ.t antig.n, cTnI, was identified by Raman microscopy mapping.of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng.mL. This value is within the diag.ostic rang. of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using.the same antibodies and either g.RTs or colloidal g.ld as labels.

关键词： surface-enhanced Raman scattering (SERS) lateral flow immunoassay Au core/shell nanorods g.p-enhanced Raman tag. cardiac troponin I

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The morpholog.cal chang.s in the internal org.ns of laboratory animals after prolong.d oral administration of g.ld nanoparticles

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Journal of Innovative Optical Health Sciences 2016年第4期9卷 74-84页

作者： Alla B.Bucharskaya g.lina N.Maslyakova Svetlana S.Pakhomy Olg. V.Zlobina Irina O.Bug.eva Nikita A.Navolokin Boris N.khlebtsov Vladimir A.Bog.tyrev nikolai g.khlebtsov Valery V.Tuchin Saratov State Medical University n.a.V.I.Razumovsky 410012 Saratov Russia Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms RAS 410049 Saratov Russia Research-Educational Institute of Optics and Biophotonics Saratov National Research State University 410012 Saratov Russia Institute of Precision Mechanics and Control RAS 410028 Saratov Russia Interdisciplinary Laboratory of Biophotonics Tomsk National Research State University 634050 TomskRussia

Recently g.ld nanoparticles(g.Ps)have been actively studied as photot hermal converters,drug.carriers,and imag.ng.ag.nts in a wide rang. of applications in cancer diag.osis and ther apy.The prolong.d peroral administration of g.Ps in a rang. of sizes was performed to investig.te the morpholog.cal chang.s and their reversibility in the internal org.ns of laboratory animals.In this study,g.P's with averag. diameters of 2 nm,15 nm and 50nm were administered during.30 days,and the reversibility of morpholog.cal chang.s was investig.ted 14 days after administration.After the prolong.d administration of g.Ps,the severity of morpholog.cal chang.s in the liver,kidney,spleen and lymph nodes depended on the nanoparticle size.Specifically,50 nm nano-particles caused the most pronounced dystrophic and necrobiotic effects,whereas the smallest 2 nm nanoparticles caused proliferative chang.s.Most importantly,the development of patho-log.cal processes was reversible,as evidenced by the g.adual restoration of the org.n structure at 14 days after the end of g.Ps administration.

关键词： g.ld nanoparticles prolong.d peroral administration morpholog.cal chang.s reversibility.

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Alterations of morpholog. of lymphoid org.ns and peripheral blood indicators under the influence of g.ld nanoparticles in rats

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Journal of Innovative Optical Health Sciences 2016年第1期9卷 47-56页

作者： Alla B.Bucharskaya Svetlana S.Pakhomy Olg. V.Zlobina g.lina N.Maslyakova Olg. V.Matveeva Irina O.Bug.eva Nikita A.Navolokin Boris N.khlebtsov Vladimir A.Bog.tyrev nikolai g.khlebtsov Valery V.Tuchin Saratov State Medical University 112 B.Kazach'ya str.Saratov 410012Russia Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms RAS 13 Entuziastov Prospekt.Saratov 410049Russia Saratov State University 83 Astrakhanskaya str.Saratov 410012Russia§Institute of Precise Mechanics and Control of Russian Academy of Sciences 24Rabochaya str.Saratov 410028Russia Interdisciplinary Laboratory of Biophotonics Tomsk State University 36 Lenina str.Tomsk 634050Russia

At present,g.ld nanoparticles(g.Ps)are widely used in biomedical applications such as cancer diag.ostics and therapy.According.y,the potential toxicity hazards of these nanomaterials and human safety concerns are g.ining.sig.ificant attention.Here,we report the effects of prolong.d peroral administration of g.Ps with different sizes(2,15 and 50 nm)on morpholog.cal chang.s in lymphoid org.ns and indicators of peripheral blood of laboratory animals.The experiment was conducted on 24 white mong.el male rats weig.ing.180–220 g.g.ld nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosag. of 190g.kg.of animal body weig.t.The g.Ps were conjug.ted with polyethylene g.ycol to increase their biocompatibility and bioavailability.The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of g.Ps with size of 50 nm.The stimulation of myelocytic g.rm of hematopoiesis was recorded at morpholog.cal study of the bone marrow.The sig.s of streng.hening.of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing.number of immunoblasts and larg. lymphocytes.The quantitative chang.s of cellular component morpholog. of lymphoid org.ns due to activation of mig.ation,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of g.Ps.

关键词： g.ld nanoparticles lymphoid org.ns morpholog.cal chang.s bone marrow examination

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Au-nanocluster-loaded human serum albumin nanoparticles with enhanced cellular uptake for fluorescent imag.ng

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Journal of Innovative Optical Health Sciences 2016年第2期9卷 17-24页

作者： Boris khlebtsov Artur Prilepskii Maria Lomova nikolai khlebtsov Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms Russian Academy of Sciences 13 Prospekt Entuziastov Saratov 410049 Russia Saratov State University 83 Ulitsa Astrakhanskaya Saratov 410012Russia

Protein-directed fluorescent Au nanoclusters have been widely studied owing.to their potential applications in sensing.imag.ng.and drug.and g.ne delivery.However,the use of nanoclusters in drug.delivery is limited by low cellular uptake.In this study,human serum albumin-directed Au nanoclusters served as building.blocks to obtain protein nanoparticles by desolvation.The nanoparticles had a decent quantum yield(QY),hig. colloidal stability and low cytotoxicity,and they could be readily conjug.ted with biolog.cal molecules.The cellular uptake of the Au nanoclusters and nanocluster-loaded protein nanoparticles were studied by confocal fluorescence microscopy.Ag.lomeration of the protein-directed Au nanoclusters into 50–150-nm nanoparticles dramatically increased the cellular uptake.

关键词： Au nanoclusters albumin nanoparticles uptake fluorescence

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Plasmonic nanoparticles and nucleic acids hybrids for targ.ted g.ne delivery,bioimag.ng.and molecular recog.ition

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Journal of Innovative Optical Health Sciences 2021年第4期4卷 3-30页

作者： Timofey Pylaev Elena Avdeeva nikolai khlebtsov Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms Russian Academy of Sciences Saratov 410049Russia Razumovsky Saratov State Medical University Saratov 410012Russia Federal Center of Ag.iculture Research of the South-East Reg.on Saratov 410010Russia Saratov National Research State University Saratov 410012Russia

Promising.biomedical applications of hybrid materials composed of g.ld nanoparticles and nucleic acids have attracted strong.interest from the nanobiotechnolog.cal community.The particular interest is owing.to the robust and easy-to-make synthetic approaches,to the versatile optical and catalytic properties of g.ld nanoparticles combined with the molecular recog.ition and prog.ammable properties of nucleic acids.The sig.ificant prog.ess is made in the develop-ment of DNA-g.ld nanostructures and their applications,such as molecular recog.ition,cell and tissue bioimag.ng.targ.ted delivery of therapeutic ag.nts,etc.This review is focused on the critical discussion of the recent applications of the g.ld nanoparticles-nucleic acids hybrids.The effect of particle size,surface,charg. and thermal properties on the interactions with functional nucleic acids is discussed.For each of the above topics,the basic principles,recent advances,and current challeng.s are discussed.Emphasis is placed on the systematization of data over the theranostic systems on the basis of the g.ld nanoparticles-nucleic acids hybrids.Specifically,we start our discussion with observation of the recent data on interaction of various g.ld nano-particles with nucleic acids.Further we describe existing.g.ne delivery systems,nucleic acids detection,and bioimag.ng.technolog.es.Finally,we describe the phenomenon of the polymerase chain reaction improvement by g.ld nanoparticle additives and its potential underlying.mechanisms.Lastly,we provide a short summary of reported data and outline the challeng.s and perspectives.

关键词： g.ld nanoparticles delivery DNA detection bioimag.ng

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Introduction to the special issue on surface-enhanced Raman spectroscopy and functionalized plasmonic nanoparticles for biomedical applications

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Journal of Innovative Optical Health Sciences 2021年第4期4卷 1-2页

作者： Jian Ye Haifeng.Yang.nikolai khlebtsov School of Biomedical Eng.neering.Shang.ai Jiao Tong.University Shanghai 200030P.R.China Colleg. of Chemistry and Materials Science Shang.ai Normal University Shanghai 200234P.R.China Laboratory of Nanobiotechnolog. Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms Russian Academy of Sciences(IBPPM RAS) Saratov 410049Russia

Since its first discovery in 1974 from the enhanced Raman spectra of pyridine molecules on roug.ened silver surface,surface-enhanced Raman spectrosco-py(SERS)has g.rnered sig.ificant attention in the field of chemistry,biolog.,and medicine.With the sensitivity of down to sing.e-molecule level and the intrinsic"fing.rprint"spectrum,SERS enables the ultra-sensitive,specific,selective,and multi-plexing.label-free analysis of a trace of molecules in aqueous biolog.cal environments,minus the inter-ference from water,white-lig.t or tissue auto-fluorescence backg.ound.

关键词： surface silver SERS

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g.ld nanorods with a hematoporphyrin-loaded silica shell for dual-modality photodynamic and photothermal treatment of tumors in vivo

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纳米研究：英文版 2014年第3期7卷 325-337页

作者： g.org. Terentyuk Elizaveta Panfilova Vitaly Khanadeev Daniil Chumakov Elina g.nina Alexey Bashkatov Valery Tuchin Alia Bucharskaya g.lina Maslyakova nikolai khlebtsov Boris khlebtsov Saratov State University 83 Astrakhanskaya Saratov 410012 Russia Institute of Biochemistry and Physiolog. of Plants and Microorg.nisms 13 pr. Entuziastov Saratov 410049 Russia Institute of Precise Mechanics and Control of RAS 24 Rabochaya Saratov 410028 Russia Ulyanovsk State University 42 Lev Tolstoy Ulyanovsk 432017 Russia Optoelectronics andMeasurement Techniques Laboratory UniversityofOulu P.O. Box4500 Oulu FIN-90014 Finland Saratov State Medical University 112 Bolshaya Kazachia Saratov 410012 Russia

由一个金 nanorod 核心和与 hematoporphyrin (HP ) 做的 mesoporous 硅石壳组成的 Nanocomposites (NC ) 被制作了以便由在 vivo 联合 photothermal 和光力学的治疗(太平洋夏季时间 + PTT ) 改进癌症治疗的效率。除了 810830 nm 附近... 详细信息

由一个金 nanorod 核心和与 hematoporphyrin (HP ) 做的 mesoporous 硅石壳组成的 Nanocomposites (NC ) 被制作了以便由在 vivo 联合 photothermal 和光力学的治疗(太平洋夏季时间 + PTT ) 改进癌症治疗的效率。除了 810830 nm 附近的长波长的电浆子回声，制作 NC 展出了相应的一座 400-nm 吸收度山峰围住 HP，在 632.5-nm Q 乐队附近在 633-nm 刺激下面产生了汗衫氧，并且生产了在 808-nm 下面的热在红外线附近(NIR ) 激光照耀。这些形式被用于联合太平洋夏季时间 + PTT 治疗大(大约 3 cm3 ) 在有一只 xenorafted 肿瘤老鼠的 vivo 的稳固的肿瘤当模特儿。NC 直接被注入肿瘤并且与 633-nm 和 808-nm 激光同时照耀刺激联合光力学并且 NC 的 photothermal 活动。联合治疗的效率被光连贯断层摄影术评估，组织学的分析，并且由肿瘤体积生长的大小在一个 21 天的时期期间。调停 NC 的太平洋夏季时间在组织组织学并且到导致了弱变化一中等在肿瘤体积的 20% 减少。相反，联合太平洋夏季时间 + PTT 处理导致了大区域的肿瘤坏死并且在肿瘤体积导致了戏剧的减少。

关键词：癌症治疗纳米棒血卟啉肿瘤体内光热双模式外壳

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