检索结果-南通市图书馆

Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel

Regenerative Biomaterials 2024年第3期3卷 56-67页

作者： ming Ke Wenhui Xu Yansha Hao Feiyang Zheng Guanyuan Yang Yonghong Fan Fangfang Wang Zhiqiang nie Chuhong Zhu department of Anatomy Third Military Medical UniversityChongqing 400038China State Key Laboratory of Trauma Burn and Combined InjuryChongqing 400038China department of Plastic and Aesthetic Surgery Southwest HospitalThird Military Medical UniversityChongqing 400038China Engineering Research Center of Tissue and Organ Regeneration and Manufacturing Ministry of EducationChongqing 400038China

Engineering myocardium has shown great clinal potential for repairing permanent myocardial ***,the lack of perfusing blood vessels and difficulties in preparing a thick-engineered myocardium result in its limited clinical *** prepared a mixed gel containing fibrin(5 mg/ml)and collagen I(0.2 mg/ml)and verified that human umbilical vein endothelial cells(HUVECs)and human-induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)could fom microvascular lumens and myocardial cell clusters by harnessing the low-hardness and hyperelastic characteristics of ***-CMs and HUVECs in the mixed gel formed self-organized cell clusters,which were then cultured in different media using a three-phase *** successfully constructed vascularized engineered myocardial tissue had a spherical structure and final diameter of *** tissue exhibited autonomous beats that occured at a frequency similar to a normal human heart *** internal microvascular lumen could be maintained for 6 weeks and showed good results during preliminary surface re-vascularization in vitro and vascular remodeling in *** summary,we propose a simple method for constructing vascularized engineered myocardial tissue,through phased cultivation that does not rely on high-end manufacturing equipment and cutting-edge preparation *** constructed tissue has potential value for clinical use after preliminary evaluation.

关键词： engineered myocardium vascularized hiPSC-CM HUVEC fibrin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phylogenetic patterns of shrub communities along the longitudinal and latitudinal gradients on the northeastern Qinghai–Tibetan Plateau

引用

Journal of Mountain Science 2020年第5期17卷 1106-1114页

作者： XIAO Yuan-ming YANG Lu-cun nie Xiu-qing LI Chang-bin XIONG Feng ZHOU Guo-ying Northwest Institute of Plateau Biology Chinese Academy of SciencesXining 810008China Key Laboratory of Tibetan Medicine Research Chinese Academy of SciencesXining 810008China Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources Xining 810008China University of Chinese Academy of Sciences Beijing 100049China

Biodiversity distribution patterns are a basic and long-standing but crucial aspect of ecology *** patterns form the primary source of data used to develop biodiversity protection practices,especially in mountain *** comprise one of the main types of vegetation on the Qinghai–Tibetan Plateau,where they serve vital ecological *** this study,we used a community phylogenetic approach to examine the distribution patterns of shrub communities along the longitudinal and latitudinal gradients on the northeastern Qinghai–Tibetan *** observed significant latitudinal trends in both the phylogenetic diversity(PD)and net relatedness index(NRI)values of shrub communities,such that the former decreased and the latter increased with increasing ***,no significant PD,NRI and nearest taxon index(NTI)distribution patterns were observed along a longitudinal gradient.A further analysis revealed that the combination of temperature-related and precipitation-related climate variables most strongly affected the PD,NRI and NTI values of shrub communities,indicating that the latitudinal patterns in the PD,NRI and NTI of a shrub community may be determined mainly by interactions with these climate factors.

关键词： Phylogenetic diversity Phylogenetic community structure Shrub communities Qinghai–Tibetan Plateau Net Relatedness Index Nearest Taxon Index

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Biocompatible and biodegradable nanoparticles for enhancement of anti-cancer activities of phytochemicals

引用

Chinese Journal of Natural Medicines 2015年第9期13卷 641-652页

作者： LI Chuan ZHANG Jia ZU Yu-Jiao nie Shu-Fang CAO Jun WANG Qian nie Shao-Ping deNG Ze-Yuan XIE ming-Yong WANG Shu department of Nutritional Sciences Texas Tech University State Key Laboratory of Food Science and Technology Nanchang University Nutrilite Health Institute department of Hematology and Oncology The First Hospital of Jilin University

Many phytochemicals show promise in cancer prevention and treatment, but their low aqueous solubility, poor stability, unfavorable bioavailability, and low target specificity make administering them at therapeutic doses unrealistic. This is particularly true for(-)-epigallocatechin gallate, curcumin, quercetin, resveratrol, and genistein. There is an increasing interest in developing novel delivery strategies for these natural products. Liposomes, micelles, nanoemulsions, solid lipid nanoparticles, nanostructured lipid carriers and poly(lactide-co-glycolide) nanoparticles are biocompatible and biodegradable nanoparticles. Those nanoparticles can increase the stability and solubility of phytochemicals, exhibit a sustained release property, enhance their absorption and bioavailability, protect them from premature enzymatic degradation or metabolism, prolong their circulation time, improve their target specificity to cancer cells or tumors via passive or targeted delivery, lower toxicity or side-effects to normal cells or tissues through preventing them from prematurely interacting with the biological environment, and enhance anti-cancer activities. Nanotechnology opens a door for developing phytochemical-loaded nanoparticles for prevention and treatment of cancer.

关键词： Nanoparticles Cancer Biocompatible Biodegradable (-)-Epigallocatechin Gallate Curcumin Quercetin Resveratrol Genistein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

引用

World Journal of Gastroenterology 2012年第37期18卷 5276-5282页

作者： Ya-ming Wei Yan-Lei Du Yu-Qiang nie Yu-Yuan Li Yu-Jui Yvonne Wan department of Blood Transfusion Guangzhou First People's Hospital Guangzhou Medical University Guang- zhou 510180 Guangdong Province China department of Gastroenterology and Hepatology Guangzhou First People's Hospital Guangzhou Medical University Guang- zhou 510180 Guangdong Province China department of Pharmacology Toxicology and Therapeutics The University of Kansas Medical Center Kansas City KS 66160 United States

AIM： To investigate the association of polymorphisms of nur-related receptor 1 （Nurrl） and development of alcohol dependence in Mexican Americans. METHODS： Peripheral blood samples were collected from 374 alcoholic and 346 nonalcoholic Mexican Amer- icans; these two groups were sex- and age-matched. Sample DNA was extracted and genomic DNA was amplified by polymerase chain reaction. The -2922（C） 2-3 polymerase chain reaction products were digested with Sau96I, alleles of 1345（G/C）, and -1198（C/G） in the regulatory region as well as Ex＋132 （G/T/A/C） and Ex＋715（T/-） in exon 3 were studied by sequencing. RESULTS： The C2/C2, C2/C3, C3/C3 genotype distribu- tion of -2922（C） 2-3 was 34.4%, 38.2% and 27.5% in the nonalcoholic group compared to 23.3%, 51.2% and 25.4% in the alcoholic group （P = 0.001）. The C/C, C/G ,G/G genotype distribution of -1198（C/6） was 23.5%, 46.1% and 30.3% in the nonalcoholic group compared to 13.9%, 50.9% and 35.3% in the alcoholic group （P = 0.007）. However, the -1345 （G/C）, Ex3＋132（G/T/A/C） and Ex3＋715（T/-） alleles were not polymorphic in Mex- ican Americans, and all those studied had G/G, G/G and T/T genotype for these three alleles, respectively. The -2922（C） 2-3 did not show allele level difference be- tween alcoholic and nonalcoholic individuals, but -1198 （C/G） showed a significant allele frequency difference between alcoholic （39.3%） and nonalcoholic （46.6%） populations （P = 0.005）. Excluding obese individuals, significant differences were found at both genotypic and allelic levels for the -2922（C） 2-3 polymorphism （P = 0.000 and P = 0.049） and the -1198 （C/G） polymor- phism （P = 0.008 and P = 0.032） between nonobese alcoholics and nonobese controls. Excluding smokers, a significant difference was found only at the genotypic level for the -2922（C） 2-3 polymorphism （P = 0.037） between nonsmoking alcoholics and nonsmoking con- trols, but only at the allelic level for the -1198（C/G） polymorphism （P = 0.034）. CONCLUSION： Polymorphisms in the r

关键词： Nur-related receptor 1 Polymorphism Al-cohol dependence Obesity Smoking Nuclear receptor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficient C_(2)-Symmetric Chiral Schiff Bases for Lanthanide-catalyzed Asymmetric Hydrogenation of Acetophenone

引用

Chinese Chemical Letters 2006年第10期17卷 1297-1300页

作者： Peng Fei YAN Wen Bin SUN Guang ming LI Chun Hong nie Zheng Yu YUE School of Chemistry and Materials Science Heilongjiang University Harbin 150080

An array of C2-symmetric chiral Schiff bases of substituted salicylaldehyde （1R, 2R）-1, 2-cyclohexanediamine and （R）-（＋）-2, 2＇-diamino-1, 1＇-binaphthalene, incorporated with lanthanide complexes, have been applied as catalysts for asymmetric hydrogenation of acetophenone for the first time. Highly enantioselective product with 90% enantiomeric excess （e.e.） was obtained when the catalyst, hybridized by the bulky electron-donating 3, 5-di-tert-butyl substituted Schiff base （2） and SmCl3.4THF, was employed.

关键词： Chiral Schiff base lanthanide (HI) complexes asymmetric hydrogenation acetophenone 2-propanol.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transient Rating Calculation of DC Filters of Jinping-Sunan ±800 kV UHVDC Project

引用

高电压技术 2012年第12期38卷 3230-3234页

作者： LI ming ZHANG Lei nie Dingzhen State Power Economic Research Institute Beijing 100052China

DC filter components and devices in converter stations of a high voltage DC transmission project may withstand a certain amount of transient overvoltage clue to the impact of transient impulses. In order to protect them, suitable arresters should be equipped properly in the filters to limit the transient overvoltage to an acceptable level. By utilizing the method of DC filter simulation and transient calculation model,the paper studies the transient overvoltage in the DC filters of the Jinping-Sunan ultra high voltage direct current(UHVDC) transmission project under the most severe transient operation conditions. Three arresters are installed inside each filter, which consequently decide the insulation levels of the filter component: mainly capacitors,reactors and resistors. The lightning impulse insulation levels for the high voltage reactors of the two filters are 648 kV and 788 kV respectively, while they are 464 kV and627 kV for the low voltage reactors, and 648 kV and 788 kV for the resistors. The insulation level for the high voltage capacitors is no more than 815 kV. The calculation results provide basis for choice of the DC filter components.

关键词：直流输电工程直流滤波器瞬态脉冲计算模型苏南瞬态过电压绝缘水平评价

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids

引用

Chinese Chemical Letters 2024年第4期35卷 481-485页

作者： Qiongqiong Wan Yanan Xiao Guifang Feng Xin Dong Wenjing nie ming Gao Qingtao Meng Suming Chen The Institute for Advanced Studies Renmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China

There is a close relationship between the biological functions of lipids and their structures, and various isomers greatly increases the complexity of lipid structures. The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we develop a visible-light-activated aziridination reaction system, which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry(LC-MS). Based on the derivatization of C=C bonds with Ph I=NTs, their location in lipids could be easily identified by tandem MS. Especially, the sn-position isomers of unsaturated phosphatidylcholine(PC) can be separated and quantified by LC-MS after the derivatization. By using the proposed method, the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed. This study offers a powerful tool for deep lipid structural biology.

关键词： Aziridination Lipid isomers C=C bond location sn-Position Mass spectrometry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hyperosmolarity disrupts tight junction via TNF-α/MMP pathway in primary human corneal epithelial cells

引用

International Journal of Ophthalmology(English edition) 2022年第5期15卷 683-689页

作者： Yun Zhang ming Yang Shi-Xin Zhao Li nie Li-Jun Shen Wei Han Eye Centre Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

AIM:To investigate the mechanism of the tight junction(TJ) disruption and the association between tumor necrosis factor(TNF)-α and matrix metalloproteinase(MMPs) under hyperosmotic condition in primary human corneal epithelial cells(HCECs).METHODS:The cultured HCECs were exposed to media which adding sodium chloride(Na Cl) for hyperosmolar stress or adding rh-TNF-α(10 ng/m L). NF-κB inhibitor(5 μmol/L) or GM-6001(potent and broad spectrum MMP inhibitor, 20 μmol/L)was added 1 h before that treatment. The integrity of TJ proteins was determined by immunofluorescent(IF) staining. The m RNA levels of TNF-α and MMPs were evaluated by quantitative reverse transcription polymerase chain reaction(RT-q PCR) and the protein expression by enzyme-linked immunosorbent assay(ELISA).RESULTS:TJ proteins ZO-1 and Occludin were disrupted in primary HCECs exposed to hyperosmotic medium. The m RNA expression and protein production of TNF-α increased significantly in hyperosmotic media at 500 m Os M. TNF-α mediated the expression and production of MMP-1, MMP-13, MMP-9, and MMP-3 stimulated by hyperosmotic stress. The production of MMPs in hyperosmolar media were increased through the increase of TNF-α. GM-6001 prevent the destruction of ZO-1 and Occludin in hyperosmolar stress and rh-TNF-α treated medium. TNF-α induced activation of MMPs was involved in the TJ disruption by ***:TJ proteins ZO-1 and Occludin are disrupted by hyperosmolar stress and TNF-α, but protected by MMP inhibitor(GM-6001). It suggests that TNF-α/MMP pathway mediates the TJ disruption in primary HCECs exposed to hyperosmotic stress.

关键词： dry eye hyperosmolarity cornea epithelium tight junction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Differences in Crystallization Behaviors between Cyclic and Linear Polymer Nanocomposites

引用

Chinese Journal of Polymer Science 2020年第9期38卷 1034-1044,I0009页

作者： Rong-Juan Liu Zhi-Ping Zhou Yong Liu Zhao-Peng Liang Yong-Qiang ming Tong-Fan Hao Yi-Jing nie Research School of Polymeric Materials School of Materials Science and EngineeringJiangsu UniversityZhenjiang 212013China Institute of Green Chemistry and Chemical Technology Jiangsu UniversityZhenjiang 212013China

Cyclic polymers exhibit fascinating crystallization behaviors owing to the absence of chain ends and more compact *** the current simulation,dynamic Monte Carlo simulations were performed to reveal the underlying mechanism of the effect of chain topology and chain length on crystallization of polymer in solutions containing one-dimensional *** results suggested that the filled cyclic polymers exhibit higher melting temperature,higher crystallization temperature,and faster crystallization rate than the analogous linear polymers of identical chain length,especially in the systems with relatively short *** on the Thomson-Gibbs equation,we theoretically analyzed the difference in the melting point between the cyclic and linear polymers under different chain lengths,and derived the dependence of the ratio of the melting point of the linear polymers to that of its cyclic analogs on chain *** addition,it was also observed that the nanofiller can induce the formation of nanohybrid shish-kebab structure during isothermal crystallization of all systems.

关键词： Cyclic polymers Linear polymers Crystallization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An online diabetic retinopathy screening tool for patients with type 2 diabetes

引用

International Journal of Ophthalmology(English edition) 2021年第11期14卷 1748-1755页

作者： Wan-Yue Li ming Yang Ya-Nan Song Ling Luo Chuang nie Mao-Nian Zhang Medical School of Chinese PLA Beijing 100853China department of Ophthalmology Chinese PLA General HospitalBeijing 100853China Medical Big Data Research Center Medical Innovation Research Division of Chinese PLA General HospitalBeijing 100853China department of Ophthalmology Strategic Support Force Medical CentreBeijing 100101China

AIM:To develop a useful diabetic retinopathy(DR)screening tool for patients with type 2 diabetes mellitus(T2DM).METHODS:A DR prediction model based on the Logistic regression algorithm was established on the development dataset containing 778 samples(randomly assigned to the training dataset and the internal validation dataset at a ratio of 7:3).The generalization capability of the model was assessed using an external validation dataset containing 128 *** DR risk calculator was developed through We Chat developer Tools using Java Script,which was embedded in the We Chat Mini ***:The model revealed risk factors(duration of diabetes,diabetic nephropathy,and creatinine level)and protective factors(annual DR screening and hyperlipidemia)for *** the internal and external validation,the recall ratios of the model were 0.92 and 0.89,respectively,and the area under the curve values were 0.82 and 0.70,***:The DR screening tool integrates education,risk prediction,and medical advice function,which could help clinicians in conducting DR risk assessments and providing recommendations for ophthalmic referral to increase the DR screening rate among patients with T2DM.

关键词： diabetic retinopathy type 2 diabetes mellitus prediction model screening

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：