Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids

作     者：Qiongqiong Wan Yanan Xiao Guifang Feng Xin Dong Wenjing Nie Ming Gao Qingtao Meng Suming Chen Qiongqiong Wan;Yanan Xiao;Guifang Feng;Xin Dong;Wenjing Nie;Ming Gao;Qingtao Meng;Suming Chen

作者机构：The Institute for Advanced StudiesRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第4期

页      面：481-485页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070304[理学-物理化学(含∶化学物理)] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China (Nos.22074111, 22004093 and 22004092) the National Key Research and Development Program of China (No.2021YFC2700700) the support of the start-up funds of Wuhan University and the National Youth Talents Plan of China 

主　　题：Aziridination Lipid isomers C=C bond location sn-Position Mass spectrometry 

摘      要：There is a close relationship between the biological functions of lipids and their structures, and various isomers greatly increases the complexity of lipid structures. The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we develop a visible-light-activated aziridination reaction system, which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry(LC-MS). Based on the derivatization of C=C bonds with Ph I=NTs, their location in lipids could be easily identified by tandem MS. Especially, the sn-position isomers of unsaturated phosphatidylcholine(PC) can be separated and quantified by LC-MS after the derivatization. By using the proposed method, the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed. This study offers a powerful tool for deep lipid structural biology.