检索结果-南通市图书馆

Journal of Sensor Technology 2024年第3期14卷 35-50页

作者： Sondes Bourigua Feriel Boussema Zayneb Jebali Houcine Barhoumi Hatem Majdoub Abderrazak Maaref nicole jaffrezic-renault Laboratory of Advanced Materials and Interfaces Faculty of Sciences University of Monastir Monastir Tunisia Institute of Analytical Sciences University of Lyon Villeurbanne France

L-tryptophan is an essential amino acid for human health. Nanofibrillated cellulose (NFC) from marram grass (Ammophila arenaria) extracted from plants harvested in the center of Tunisia was used for the first time for the modification of a glassy carbon electrode (GCE), for the sensitive detection of L-tryptophan (Trp). After spectroscopic and morphological characterization of the extracted NFC, the GC electrode modification was monitored through cyclic voltammetry. The NFC-modified electrode exhibited good analytical performance in detecting Trp with a wide linear range between 7.5 × 10−4 mM and 10−2 mM, a detection limit of 0.2 µM, and a high sensitivity of 140.0 µA∙mM−1. Additionally, the NFC/GCE showed a good reproducibility, good selectivity versus other amino acids, uric acid, ascorbic acid, and good applicability to the detection of Trp in urine samples.

关键词： Nanofibrillated Cellulose Chitosan Chemically Modified Glassy Carbon Electrode Electrochemical Detection L-Tryptophan

Biosorption of cadmium(II) and lead(II) ions from aqueous solutions onto dried activated sludge

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2006年第5期18卷 840-844页

作者： WANG Xue-jiang XIA Si-qing CHEN Ling ZHAO Jian-fu CHOVELON Jean-marc nicole jaffrezic-renault State Key Laboratory of Pollution Control and Resources Reuse Yongji University Shanghai 200092 China Application of Chemistry and Environment Laboratory UMR CNRS 5634 University Claude Bernard Lyon I 43 Boulevard du 11 Novemvre 1918Villeurbanne Cedex 69622 France CEGELY UMR/CNRS 5005 Central University of Lyon 69134 Ecully Cedex France

The removal of heavy-metal ions from aqueous solutions by using dried activated sludge has been investigated in batch systems. Effect of solution pH, initial metal ion concentration, and temperature were determined. The results of the kinetic studies showed that the uptake processes of the two metal ions（Cd（Ⅱ） and Pb（Ⅱ）） followed the pseudo-second-order rate expression. The equilibrium data fitted very well to both the Langmuir and Freundlich adsorption models. The FT-IR analysis showed that the main mechanism of Cd（Ⅱ） and Pb（Ⅱ） biosorption onto dried activated sludge was their binding with amide I group.

关键词： biosorption adsorption isotherm cadmium lead activated sludge

A Simple Over-Oxidized Molecularly Imprinted Polypyrrole for the Sensitive Detection of Dopamine in Human Serum

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Sensor Technology 2022年第3期12卷 33-44页

作者： Sarra Slimi Chama Mabrouk Houcine Barhoumi nicole jaffrezic-renault Laboratory of Advanced Materials and Interfaces Faculty of Sciences University of Monastir Monastir Tunisia Institute of Analytical Sciences University of Lyon Villeurbanne France

A simple electrochemical sensor for dopamine detection, is based on molecularly imprinted and electropolymerized over-oxidized polypyrrole (OPPy). The MIP-based electrode is obtained by electrocopolymerization of pyrrole (0.1 M) in the presence of the template molecular (dopamine, DA) (10-3 M). The square wave voltammetry (SWV) is used for the detection of dopamine in buffer solution. The current peak obtained at the MIP electrode was proportional to the logarithm of the DA concentration in the range of 10-11 to 5 × 10-8 M with a detection limit of 10-11 M. The proposed sensor was used for the detection of DA in spiked blood serum, satisfactory results were obtained.

关键词： Dopamine Molecularly Imprinted Polymers Over-Oxidized Polypyrrole Square Wave Voltammetry

Characterization and Classification of Groundwater from Wells Using an Electronic Tongue (Kairouan, Tunisia)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2011年第7期3卷 531-539页

作者： Khawla Sghaier Houcine Barhoumi Abderrazak Maaref Maryam Siadat nicole jaffrezic-renault Laboratoire de Physique et Chimie des Interface Faculté des Sciences de Monastir 5000 Tunisia

A sensor array comprising 9 potentiometric chemical sensors and some pattern recognition tools for the data processing has been applied in order to characterize the groundwater in the plain of Kairouan Region (Tunisia). A total of 17 groundwater samples were collected from three different villages and analyzed for their chemical components. Nine chemical parameters were determined: Potassium, Sodium, Calcium, Ammonium, Cadmium, Chlorides, Nitrates, Fluoride and pH. Multi-sensor responses measured in each water sample were diagnosed by Principal Component Analysis (PCA) and Cluster Analysis (CA). PCA is a procedure for reducing data redundancy. CA is used to detect spatial similarity among sampling sites. This methodology is simple, rapid and the obtained results demonstrate that the electronic tongue technique based on the sensor array combined with pattern recognition method could be a useful tool for the characterization and the classification of wells water samples.

关键词： Electronic Tongue Groundwater Pattern Recognition Tools PCA CA

An Electrochemical Nitrite Sensor Based on a Multilayer Film of Polyoxometalate

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sensor Technology 2013年第3期3卷 84-93页

作者： Yosra Sahraoui Sana Chaliaa Abderrazak Maaref Amor Haddad nicole jaffrezic-renault Laboratory of Interfaces and Advanced Materials Faculty of Sciences of Monastir Monastir Tunisia Laboratory of Materials and Crystallochemistry Superior Institute of Applied Science and Technology Mahdia Tunisia University of Lyon Institute of Analytical Chemistry Villeurbanne France

In this work, we have developed an electrochemical sensor for nitrite detection, based on a polyoxometalate (POM) namely mono-lacunary keggin anion [SiW11O39]8﹣ cited as (SiW11). Electrochemical characterization of SiW11 shows two-step reduction processes, with formal potentials of ﹣0.5 V (I) and ﹣0.68 V (II). Oppositely charged polyelectrolyte (poly (allylamine hydrochloride) (PAH)) and (SiW11) were assembled alternately to modify glassy carbon electrode. The electrochemical behavior of the modified electrode was studied in detail using cyclic voltammetry (CV). The results showed that SiW11/PAH/GC electrode present good electrocatalytic activity for the reduction of nitrite. The sensor showed a dynamic range from 100 μM to 3.6 mMof nitrite and no interference from other classical anions. Experimental factors that affect electron-transfer rate in these films, such as pH effect and layers number, were systematically analyzed.

关键词： Nitrite Sensor Polyoxometalate Mono-Lacunary Keggin Anion Layer by Layer Assembly Process

Adjunction of Avidin to a Cysteamine Self-Assembled Monolayer for Impedimetric Immunosensor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biomaterials and Nanobiotechnology 2016年第1期7卷 1-12页

作者： Oussama Ouerghi M. Fethi Diouani A. Belkacem A. Elsanousi nicole jaffrezic-renault Prince Sattam Bin Abul Aziz University AlKharj Kingdom of Saudi Arabia Laboratory of Epidemiology and Veterinary Microbiology (LR11IPT03 ) Pasteur Institute of Tunis Tunis Tunisia Institute of Analytical Sciences University of Lyon UMR CNRS 5280 Villeurbanne France

In this work an impedimetric immunosensor based on affinity immobilization method of a biotin labelled anti-human IgG antibody, used as a model system, was reported. The experimental procedure involves the growth of a self-assembled monolayer of a thiol (cysteamine) carrying terminal amine groups on gold electrodes. Glutarardehyde, a homobifunctional cross-linker, was used as a coupling reagent for the covalent linking of avidin to the amine groups of cysteamine. The attachment of the biotin labeled antibodies (anti-Human IgG) to the subsequent modified gold electrode was achieved by affinity interactions tacking advantage of the strong avidin-biotin bridge. The stepwise assembly process of the electrode was interrogated by means of cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurements. The response of the antibody modified electrode to their target antigens was investigated in the presence of BSA (bovin serum albumin) in order to alleviate non-specific adsorption problems. A proposed electrical model was used to analyse the experimental data. The resulting immunosensor has a linear dynamic range of 100 - 900 ng?ml–1 of antigen and a detection limit of 100 ng?ml–1.

关键词： Avidin Cysteamine Electrochemical Impedance Spectroscopy Immunosensor

Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in <i>Arthrospira platensis</i>

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2014年第5期5卷 441-453页

作者： Nadèje Tekaya Olga Saiapina Hatem Ben Ouada Florence Lagarde Philippe Namour Hafedh Ben Ouada nicole jaffrezic-renault Institute of Analytical Sciences University of Lyon Villeurbanne France Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Kyiv Ukraine Laboratory of Interfaces and Advanced Materials Faculty of Sciences University of Monastir Monastir Tunisia National Institute of Marine Sciences and Technologies University of Monastir Monastir Tunisia

An original bi-enzymatic biosensor was designed by immobilizing Arthrospira platensis cells, called Spirulina, on gold interdigitated transducers. Phosphatase and esterase activities were inhibited, respectively, by heavy metals and by pesticides. Inhibition activities were observed with different mixtures of pesticides + heavy metals. The quantification limits for Cd2+ and Hg2+ are 10-20 M in mixture and in pure solution. The quantification limits of parathion-methyl, paraoxon-methyl and triazine are respectively 10-20 M, 10-18 M and 10-20 M in mixture and pure solutions. These results show that there is no synergistic effect between the two families of pollutants. Qualitative contamination of effluent samples and their purification after passing through a municipal wastewater treatment plant were observed by our bi-enzymatic biosensor and confirmed by classical analytical techniques for heavy metal ions.

关键词： Inhibition Phosphatase Activity Esterase Activity Conductometry Arthrospira platensis

A Novel 3-D Nano-assembly Bacteria Based Biosensor for Enhanced Detection of Heavy Metal Pollutants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Science and Engineering(A) 2012年第7期1卷 924-935页

作者： Mina Souiri Ibtissem Gammoudi Laurence Mora Hafedh Ben Ouada Thierry Jouenne nicole jaffrezic-renault Corinne Dejous Ali Othmane Anthony C. Duncan Biophysic Laboratory LIMA Faculty of Medicine of Monastir University of Monastir Monastir 5019 Tunisia Department of Bioengineering of Cardiovascular Polymers Galilee Institute Villetaneuse 93430 France Laboratory of the Interfaces of the Advanced Materials Faculty of Sciences of Monastir Monastir 5000 Tunisia Proteomic Platform Faculty of Sciences of Rouen Mont Saint A ignan Cedex 76821 France Analytical Sciences Laboratory Claude Bernard Lyon 1 University Villeurbanne Cedex 69622 France University of Bordeaux Talence 33405 France

Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficult but important task. The current major limitation lies in the poor detection limits of the targeted pollutant＇s trace concentrations by the available conventional techniques. In order to elaborate a novel ＂living＂ self assembled electrochemical 3-D biosensor, the authors propose a new concept to overcome this shortcoming. The advantages of the properties of polyelectrolyte-functionalized NBs （nanobeads） are combined along with the use of non covalently strongly bound micro-organisms. The designed 3-D biosensor is all the more promising as it has showed a significantly improved sensitivity. In fact, the detection limits of the tested heavy metals （cadmium and mercury） were as low as 1.0 × 10^-12 mol.L-1 and six to seven orders of magnitude lower than those provided by conventional 2-D biosensors. Furthermore, it is potentially applicable to a wide range of bioreceptor-pollutant detection systems.

关键词： Biosensor nanobeads PEM heavy metals E. coli EIS (electrochemical impedance spectroscopy).

Long-term follow-up of patients with congenital diaphragmatic hernia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pediatric Surgery 2024年第2期7卷 100-109页

作者： nicole Cimbak Terry L Buchmiller Department of Pediatric Surgery Boston Children's HospitalBostonMAUSA

Neonates with congenital diaphragmatic hernia encounter a number of surgical and medical morbidities that persist into *** mortality improves for this population,these survivors warrant specialized follow-up for their unique disease-specific *** congenital diaphragmatic hernia clinics are best positioned to address these complex long-term morbidities,provide long-term research outcomes,and help inform standardization of best practices in this cohort of *** review outlines long-term morbidities experienced by congenital diaphragmatic hernia survivors that can be addressed in a comprehensive follow-up clinic.

关键词： hernia congenital patients

Validation of a conductometric bienzyme biosensor for the detection of proteins as marker of organic matter in river samples

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2009年第4期21卷 545-551页

作者： KHADRO Basma NAMOUR Philippe BESSUEILLE Franois LEONARD Didier jaffrezic-renault nicole University of Lyon Laboratory of AnalyticalCNRS UMR 5180 69622 Villeurbanne France Cemagref of Lyon

This article describes a conductometric bi-layer based bienzyme biosensor for the detection of proteins as a marker of organic matter in rivers. Proteins were chosen to be used as indicators of urban pollution. The working mechanism of the bienzyme biosensor is based on the enzymatic hydrolysis of proteins into several fractions （peptides and amino acids）, which results in a local conductivity change depending of the concentration of proteins. In this work, we began with the optimization of biosensor response using bovine serum albumin （BSA） as standard protein. For this objective seven enzymatic biosensors were prepared： four enzymatic sensors with only one layer of enzyme （proteinase K, trypsin, pronase or protease X） and three other enzymatic sensors with two layers （first layer： membrane containing proteinase K; second layer： one of the three other enzymes： trypsin, pronase or protease X）. The biosensors were obtained through the deposition of enzymatic layers and the cross-linking process between enzymes and BSA in saturated glutaraldehyde vapour. The response of the various biosensors, described previously, were compared with the values of total organic carbon （TOC）, and those of organic nitrogen （No~）, as determined by the laboratory accredits （CEMAGREF of Lyon） using the traditional method of analysis （NF EN 1484, infrared spectroscopy） and （NF EN 25663, mineralization/colorimetry assay） respectively for each water sample obtained from different sites in Lyon （France）. The linear correlations obtained with the response of the seven biosensors showed the most important indices of correlations for the biosensor with two enzymatic layers： proteinase K ＋ pronase （pkp）. The optimum conditions for the preparation of the pkp biosensor increased the sensitivity and gave a limit of quantification of 0.583 μg/L for TOC and 0.218 μg/L for Norg in water samples. This sensor shows good reproducibility （2.28%）, a capacity to be used at temperatures range 10-- 30℃ （dependi

关键词： conductometric biosensor proteins enzyme natural water analyses total organic carbon organic nitrogen