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Diagnosis and characterization of the ribosomal DNA-ITS of potato rot nematode(Ditylenchus destructor) populations from Chinese medicinal herbs

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Journal of Integrative Agriculture 2023年第6期22卷 1763-1781页

作者： ni chun-hui HAN Bian LIU Yong-gang Maria MUNAWAR LIU Shi-ming LI Wen-hao SHI Ming-ming LI Hui-xia PENG De-liang College of Plant Protection Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China Institute of Plant Protection Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China Department of Biological Sciences University of LethbridgeLethbridge 4401Canada State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.

关键词： Ditylenchus destructor minisatellites ITS-RFLP phylogeny RNA secondary structure

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Study on the Substituent Effects on the NMR Chemical Shifts of 2,5-disubstituted Pyrimidines

Study on the Substituent Effects on the NMR Chemical Shifts ...

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中国化学会第十二届全国物理有机化学学术会议

作者： Hua Yuan Yan Zhang chun-ni Chen Key Laboratory of Theoretical Organic Chemistry and Function Molecule Ministry of EducationHunan Provincial University Key Laboratory of QSAR/QSPRSchool of Chemistry and Chemical EngineeringHunan University of Science and Technology

The substituent cross-interaction effect in the substituted benzylidene anilines(Y-p)has been observed and widely *** order to investigate whether the substituent crossinteraction effect exist in all the conjugated sy...

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Cardioprotective Potential of Cymbopogon citratus Essential Oil against Isoproterenol-induced Cardiomyocyte Hypertrophy:Possible Involvement of NLRP3 Inflammasome and Oxidative Phosphorylation Complex Subunits

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Current Medical Science 2024年第2期44卷 450-461页

作者： Xiao-yun DING Hao ZHANG Yu-mei QIU Meng-die XIE Hu WANG Zheng-yu XIONG Ting-ting LI chun-ni HE Wei DONG Xi-lan TANG School of Pharmacy Jiangxi Science&Technology Normal UniversityNanchang 330013China Key Laboratory of Modern Preparation of Chinese Medicine Ministry of EducationJiangxi University of Chinese MedicineNanchang 330004China Jiangxi Provincial Key Laboratory of Drug Design and Evaluation Nanchang 330013China

Objective:Cymbopogon citratus(DC.)Stapf is a medicinal and edible herb that is widely used for the treatment of gastric,nervous and hypertensive *** this study,we investigated the cardioprotective effects and mechanisms of the essential oil,the main active ingredient of Cymbopogon citratus,on isoproterenol(ISO)-induced cardiomyocyte ***:The compositions of Cymbopogon citratus essential oil(CCEO)were determined by gas chromatography-mass *** were pretreated with 16.9µg/L CCEO for 1 h followed by 10µmol/L ISO for 24 *** hypertrophy-related indicators and NLRP3 inflammasome expression were ***,transcriptome sequencing(RNA-seq)and target verification were used to further explore the underlying ***:Our results showed that the CCEO mainly included citronellal(45.66%),geraniol(23.32%),and citronellol(10.37%).CCEO inhibited ISO-induced increases in cell surface area and protein content,as well as the upregulation of fetal gene ***,CCEO inhibited ISO-induced NLRP3 inflammasome expression,as evidenced by decreased lactate dehydrogenase content and downregulated mRNA levels of NLRP3,ASC,CASP1,GSDMD,and IL-1β,as well as reduced protein levels of NLRP3,ASC,pro-caspase-1,caspase-1(p20),GSDMD-FL,GSDMD-N,and pro-IL-1β.The RNA-seq results showed that CCEO inhibited the increase in the mRNA levels of 26 oxidative phosphorylation complex subunits in ISO-treated *** further experiments confirmed that CCEO suppressed ISO-induced upregulation of mt-Nd1,Sdhd,mt-Cytb,Uqcrq,and mt-Atp6 but had no obvious effects on mt-Col ***:CCEO inhibits ISO-induced cardiomyocyte hypertrophy through the suppression of NLRP3 inflammasome expression and the regulation of several oxidative phosphorylation complex subunits.

关键词： Cymbopogon citratus essential oil cardiac hypertrophy NLRP3 inflammasome oxidative phosphorylation complex subunits

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脑卒中后白质完整性与上肢运动功能的关系

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中国医学影像技术 2009年第12期25卷 2193-2196页

作者：邱明国 chun chun ni Justin RAJENDRA Andrew J. BUTLER 第三军医大学生物工程与医学影像学院医学信息与医学图像学教研室重庆400038 Department of Rehabilitation Medicine School of Medicine Emory University Atlanta GA 30322

目的应用扩散张量成像技术测量偏瘫患者内囊后肢的各向异性(FA)值,分析皮质脊髓束完整性与患侧上肢运动功能的关系。方法选择17例亚急性期脑卒中后偏瘫患者,测量患侧及健侧内囊后肢FA值,并计算FA非对称比值,临床检测包括上肢运动功能指... 详细信息

目的应用扩散张量成像技术测量偏瘫患者内囊后肢的各向异性(FA)值,分析皮质脊髓束完整性与患侧上肢运动功能的关系。方法选择17例亚急性期脑卒中后偏瘫患者,测量患侧及健侧内囊后肢FA值,并计算FA非对称比值,临床检测包括上肢运动功能指标(WMFT)及运动功能、平衡、感觉及关节功能综合评价指标(FM),利用线性相关分析FA非对称比值与患手FM评分、WMFT非对称比值及log WMFT之间的相关关系。结果患手运动功能评分与内囊后肢FA非对称比值显著相关,FA非对称性比值与患手FM(R2=0.655,P<0.002)呈线性负相关,与WMFT非对称性比值(R2=0.649,P<0.002)、lgWMFT(R2=0.636,P=0.002)以及握力非对称性比值(R2=0.414,P=0.02)均呈线性正相关。结论偏瘫患者亚急性期皮质脊髓束完整性与上肢运动功能显著相关;利用扩散张量成像技术可早期监测皮质脊髓束的完整性。

关键词：磁共振成像部分各向异性值运动功能

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物流通道的空间溢出效应检验——基于中国11条物流通道的实证

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交通运输系统工程与信息 2018年第1期18卷 37-43页

作者：范月娇权春妮华侨大学工商管理学院福建泉州362021

以中国11条物流通道为实证对象,综合测算物流通道技术水平,构造基于地理距离的空间邻接权重矩阵,检验相关变量的空间自相关性,构建空间杜宾面板数据模型验证了中国物流通道的空间溢出效应.研究表明:中国物流通道总体上具有显著的正向空... 详细信息

以中国11条物流通道为实证对象,综合测算物流通道技术水平,构造基于地理距离的空间邻接权重矩阵,检验相关变量的空间自相关性,构建空间杜宾面板数据模型验证了中国物流通道的空间溢出效应.研究表明:中国物流通道总体上具有显著的正向空间溢出效应,但从分布于不同空间的物流通道看,长江、沿海、京九、陇海兰新和沪昆物流通道正向空间溢出效应显著;而京沪、京广和宝昆物流通道研究期内空间溢出效应不显著.我国物流通道的空间溢出效应具有显著的空间差异,应因地制宜地合理化建设物流通道.

关键词：交通运输经济空间溢出效应空间杜宾面板数据模型物流通道空间差异

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词体革命:创作思路与理论建构

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兰州大学学报（社会科学版） 2012年第1期40卷 31-40页

作者：倪春军复旦大学中文系上海200433

以梁启超等人为代表的"诗界革命"的发起者和参与者,没有进行词体革命,这不仅表现为创作的贫乏,更表现为理论的缺失。而梁启超前期以"雅化"的标准推尊词体,也许是他没有进行词体革命的内在因素。胡适提出"白话词"理论,应该为现代文学史... 详细信息

以梁启超等人为代表的"诗界革命"的发起者和参与者,没有进行词体革命,这不仅表现为创作的贫乏,更表现为理论的缺失。而梁启超前期以"雅化"的标准推尊词体,也许是他没有进行词体革命的内在因素。胡适提出"白话词"理论,应该为现代文学史上词体的第一次革命,但还只是文学革命的附庸。曾今可发起的"词的解放"运动,是"诗界革命"以来第一次以词为主体的革命,取得了理论和实践的双重丰收;但因为未能领会诗界革命"新意境"的内涵,从而脱离了时代。李冰若和卢冀野之"旧辞新境"的改革,取得了理论和创作的成功,是最接近梁启超"诗界革命"精神的一次词体革命。词体革命经历了四个阶段,经过了三代学人的传递,呈现出不同的词体定位意识,导致各家理论无法调和,很难创造出各种要素完美结合的新词体。但词体革命在语言、格律、意境、风格等方面的探索,对当代词坛具有重要的启示。

关键词：词体革命词体缺席白话词词的解放旧辞新境

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基于亥姆霍兹定理计算动力学系统的哈密顿能量函数

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物理学报 2016年第24期65卷 30-35页

作者：王春妮王亚马军兰州理工大学理学院物理系兰州730050

亥姆霍兹定理表明任意空间矢量场可以分解为涡旋场和梯度场的叠加.由于电磁场变化和电磁波传播则导致电磁场能量的迁移,动力学振子和神经元处于复杂电磁环境下必然伴随能量的吸收和释放.在非线性混沌电路、电容器充电放电以及电感线圈... 详细信息

亥姆霍兹定理表明任意空间矢量场可以分解为涡旋场和梯度场的叠加.由于电磁场变化和电磁波传播则导致电磁场能量的迁移,动力学振子和神经元处于复杂电磁环境下必然伴随能量的吸收和释放.在非线性混沌电路、电容器充电放电以及电感线圈感应过程中都伴随着能量的转换和迁移.包含量纲的非线性振荡电路可利用标度变换方法转换为无量纲的动力学方程.利用平均场理论,电场能量和磁场能量的转换可用若干非线性振荡电路的动力学方程来刻画.基于亥姆霍兹定理来研究一类无量纲非线性动力学系统的哈密顿能量计算问题,对于实际的非线性振荡电路,通过标度变换可快速计算其能量函数.该结果对于动力学系统自适应控制有重要的参考价值.

关键词：亥姆霍兹定理能量函数振荡电路混沌系统

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Influence of CD133^+ expression on patients' survival and resistance of CD133^+ cells to anti-tumor reagents in gastric cancer

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 996-1004页

作者： De-Hu Chen Rui-Qi Lu Xiao-chun ni Ju-Gang Wu Shou-Lian Wang Bo-Jian Jiang Ji-Wei Yu Department of General Surgery Third People's Hospital Shanghai Jiao Tong University School of Medicine

Objective:To investigate the influence of CD133+expression on patients'survival and resistance of CD133+cells to anti-tumor agents in gastric cancer(GC).Methods:Influence of CD133 expression on prognosis was analyzed employing samples from patients with *** cell lines were utilized to separate CD133+and CD133-subpopulations by immunomagnetic separation and to analyze the biological features of two subpopulations in vitro and in vivo,especially in resistant to anti-tumor reagents and its apoptotic ***:The lower CD133+group showed a significantly better survival compared with the higher CD133+*** highest content of CD133+subpopulations for KATO-III cells had stronger proliferative ability than CD133-subpopulations.A single CD133+cell was capable of generating new cell colony and the tumorigenicity rate in nude mice was100%for CD133+clonal spheres or for CD133+cells,but 0%for ***,the higher expression levels of Oct-4,Sox-2,Musashi-1 and ABCG2 in CD133+clonal spheres were identified compared with CD133+cells or *** the treatment of anti-tumor reagents,CD133+cells had lower suppression rates compared with CD133-cells while lower level of Bcl-2 and higher level of Bax were found in CD133+cells compared with ***:The patients with lower CD133+expression had a better *** CD133+cells in clonal sphere shared the ability to be self-renewable,proliferative,tumorigenic and resistant to anti-tumor agents as probably regulated by Bcl-2 and Bax.

关键词： Stomach Cancer CD133 Tumor initiating cells

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HPLC Determination of Ascorbic Acid Using Luminol-Cu(Ⅱ) Chemiluminescence-Application to the Analysis of Juice Beverage

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Wuhan University Journal of Natural Sciences 2000年第3期5卷 339-341页

作者： Wu Feng-wu, ni chun-lin, He Zhi-ke, Luo Qing-yao, Zeng Yun-e College of Chemistry and Environmental Science, Wuhan University, Wuhan 430072, China Wuhan University College of Chemistry and Environmental Science Wuhan China (GRID:grid.49470.3e) (ISNI:***)

The chemiluminescence (CL) of luminol-Cu(Ⅱ) was applied to HPLC determination o f ascorbic acid, which was separated by a C 18 reverse-phase column with a mobile phase of 0.25 mol/L HAc. The eluted ascorbic acid was mixed with 0.3 mmol/L l um inol and 0.05 mol/L CuSO 4. The light emission from the reaction of Cu(Ⅱ) oxi diz ed ascorbic acid and luminol was detected by a modified luminometer. The detecti on limit was 3.6×10 -6 mol/L for ascorbic acid at a S/N ratio of 3, and the linea r calibration range was 2×10 -4\| 2×10 -3 mol/L. The relative standard deviation for 5 replicate injections of 1×10 -3 mol/L ascorbic acid was calculated as 4.3 % . The method was successfully applied to determination of ascorbic acid in juice beverage.

关键词： ascorbic acid chemiluminescence lumin ol HPLC beverage

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The Substituent Effects on the UV Absorption Energy of 2,5-disubstituted Pyrimidines

The Substituent Effects on the UV Absorption Energy of 2,5-d...

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中国化学会第十二届全国物理有机化学学术会议

作者： Yan Zhang chun-ni Chen Meng-Yang Li Hua Yuan Key Laboratory of Theoretical Organic Chemistry and Function Molecule Ministry of EducationHunan Provincial University Key Laboratory of QSAR/QSPRSchool of Chemistry and Chemical EngineeringHunan University of Science and Technology

Pyrimidine-containing molecules have been extensively investigated in organic light emitting devices(OLEDs),solar cells,liquid crystalsand etc due to their perfect photoelectric ***(UV)absorption is one of the most interested properties by *** investigation of the substituent effects on the UV absorption energy of the pyrimidine derivatives will guide to design functional molecules with

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