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Carcass image segmentation using CNN-based methods

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Information Processing in Agriculture 2021年第4期8卷 560-572页

作者： Diogo Nunes Goncalves Vanessa aparecida de Moares Weber Julia Gindri Bragato Pistori Rodrigo da costa Gomes Anderson Vicoso de Araujo Marcelo Fontes pereira Wesley Nunes Goncalves Hemerson Pistori Federal University of Mato Grosso do Sul Av.Costa e Silvas/nBairro UniversitarioCampo GrandeMS 79070-900Brazil INOVISAO Dom Bosco Catholic UniversityAvenida Tamandare6000CP 100Jardim SeminarioCampo GrandeMS 79117-900Brazil EMBRAPA Gado de Corte Av.Radio Maia830Vila PopularCampo GrandeMS 79106-550Brazil State University of Mato Grosso do Sul Av.Dom Antonio Barbosa4155Vila Santo AmaroCampo GrandeMS 79115-898Brazil

Carcass grading can be used as an important metric to determine meat ***,carcass grading is usually performed by a specialist,making it a subjective and errorprone *** increase the accuracy of such task,image-based systems have been proposed in the *** of the most important parts of an image-based system is the image segmentation,which aims to identify the regions of the carcass in the *** this paper,we propose the use of two recent image segmentation methods called Superpixel+CNN(Convolutional Neural Network)and *** evaluate both methods,we have also built a dataset of carcass images and their *** of approximately 96%of pixel accuracy show the robustness of the methods in carcass image *** novelty of this work is the proposal and comparison of recent deep learning methods that use CNN and superpixels in carcass *** this way,the methods can be used in carcass grading systems to increase the accuracy of the grading task.

关键词： Carcass grading Image segmentation Convolutional neural networks Superpixels

Chlorophyll Fluorescence as an Indicator of Cellular Damage by Glyphosate Herbicide in Raphanus sativus ***

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American Journal of Plant Sciences 2014年第16期5卷 2509-2519页

作者： Fabia Barbosa Silva Alan Carlos costa Rodolfo Rodrigo pereira Alves Clarice aparecida Megguer Instituto Federal de Educacao Ciencia e Tecnologia—Campus Rio VerdeRio VerdeBrazil

The fodder radish is an important alternative late summer crop in central-western Brazil. The fodder radish has a short growing cycle, is highly productive and possesses qualities that enhance its cultivation potential as an alternative in Brazil’s Biodiesel Program. However, drift of herbicides, such as glyphosate, may hinder the development of fodder radishes. Studies of chlorophyll fluorescence can provide measurements that help assess the effect of herbicide, being good indicator of the damage to photosynthetic apparatus promoted by herbicide. This study was carried out to analyze the effects of glyphosate by measuring chlorophyll a fluorescence, cellular membrane stability and chloroplast pigment contents in fodder radish plants. This experiment used a randomized block design in which the treatments consisted of the following 6 different doses: control, 0, 150, 300, 450, 600, 750 and 900 g i.a. ha-1 of the recommended 480 g·L-1 dose of glyphosate acid equivalent. The plants were sprayed 30 days after emergence and were assessed every 48 hours. The characteristics evaluated, including chlorophyll a fluorescence, chloroplast pigments and the rate of electrolyte leakage, indicated that radish plants are sensitive to glyphosate because the treatments resulted in reduced levels of photosynthetic efficiency and increased damage to cellular membranes, which led to a reduction in the chloroplast pigment content.

关键词： Pigments Chloroplastidic EPSPS Chlorophyll Fluorescence

In Vitro Propagation of Grapevine Cultivars with Potential for South of Brazil

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American Journal of Plant Sciences 2015年第11期6卷 1806-1815页

作者： Jean Carlos Bettoni Murilo Dalla costa Joao Peterson pereira Gardin Aike Anneliese Kretzchmar Juliana aparecida Souza University of the State of Santa Catarina UDESC/CAVLagesBrazil Company of Agriculture Research and Rural Extension of Santa Catarina EPAGRIExperimental StationLages and VideiraBrazil

The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and P?l?skei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.

关键词： IAC 571-6 Poloskei Muskotaly In Vitro Culture Vitis Culture Medium

Production of Peanut Intercropped with Forage Palm in Pernambuco State, Brazil

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American Journal of Plant Sciences 2015年第6期6卷 818-825页

作者： Juliana aparecida Santos Andrade Jeandson Silva Viana José Jairo Florentino Cordeiro Junior Abraao Cicero da Silva Edilma pereira Goncalves Djayran Sobral costa Academic Unit Garanhuns Federal Rural University of PernambucoRecifeBrazil

The greater use of crop fields by increasing plant population is an alternative to minimize losses to the farmers who use crop intercropped provides other benefits such as lower incidence of diseases and pests, and better exploitation and enrichment agroecosystem. The objective of this study was to evaluate the effect of the population of peanuts with and without inoculation and intercropping with forage palm on growth, productivity and biomass peanut. The treatments consisted of different populations of peanut (one, two and three rows of peanut row spacing of two meters of cactus pear), with and without inoculant, analyzed in subdivided plot scheme (3 × 2), totalizing six treatments in a randomized block design with four replications. The variables evaluated were plant height, number of branches, shoot dry mass and root nodule number, nodule dry weight, biomass productivity and legumes. The largest population of plants in number of lines of peanut intercropped with forage cactus promotes greater total biomass production and yield of beans and does not alter the height, branch number, dry mass of aerial part roots and nodules. The inoculant application did not result in to increased productivity biomass and growth of peanut plants, their use is not indicated. The planting of a row of peanuts between the rows of palm is not economically recommended.

关键词： Arachis hypogaea L. Inoculation Population Nopalea cochenillifera

Determination of Paraquat in Several Commercially Available Types of Rice

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Food and Nutrition Sciences 2018年第12期9卷 1368-1375页

作者： Thais Lopes Lima Maria aparecida Nicoletti Camila Munhoz Gabriel Ramos De Abreu Julia Zaccarelli Magalhaes Esther Lopes Ricci Ricci Paula A.Faria Waziry Júlia nathalia Alves da costa Ana Carolina Nascimento Antonio AndréRinaldi Fukushima Universidade Sao Judas Tadeu-R.Taquari Sao Paulo/SPBrazil Departamento de Farmacia.Faculdade de Ciencias Farmaceuticas.Universidade de Sao Paulo Sao Paulo/SPBrazil Instituto de Ciencias da Saude Universidade Presbiteriana MackenzieRua da ConsolacaoSao Paulo/SPBrazil Departamento de Patologia Faculdade de Medicina Veterinaria e ZootecniaUniversidade de Sao PauloSao Paulo/SPBrazil New Southeastern University Fort LauderdaleFloridaUnited States of America Veterans Affairs Medical CenterMiamiFloridaUSA Faculdade Sao Bento-Largo de Sao Bento s/no-CentroSao Paulo-SPSao Paulo/SPBrazil

Introduction: Paraquat (1,1’-dimethyl-4,4’-bipyridine-dichloride) is a herbicide used to combat weeds and improve crop productivity. Its acute toxicity can cause fatal poisoning in humans and animals and several studies have shown a strong correlation between paraquat and Parkinson’s disease. Objective: To determine the concentration of paraquat in different types of rice in Brazil. Method: Paraquat quantification is based on a complexation reaction with sodium dithionite, which generates a blueish color compound, whose absorbance was read in a spectrophotometer at the 600 nm wavelength. Results: Five samples were found to be above the maximum allowed limit, while one sample had a concentration in the upper allowed limit. The method employed did not allow quantification of black rice. Conclusion: There is a need for identification of the type of pesticide used in each examined crop as well as their respective dates of remission in order to improve the safety of agricultural practices.

关键词： Paraquat Herbicide Rice

Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

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Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das Neves Saez Roxelle Ethienne Ferreira Munhoz Naiara Climas pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire costa Brancalhao Maria aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto Naiara Climas pereira Claudia Regina das Neves Saez Rafaela Bespalhuk Veronica Aureliana Fassina Graziele Milani Pessini Mayarha Patricia Dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole

Approach to Paroxysmal Atrial Fibrillation Type Arrhythmia in a Cardiology Service:A Case Report

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Modern Research in Inflammation 2020年第2期9卷 9-17页

作者： Miriam Viviane Baron Luis Manuel Ley Dominguez Carolina Paz Mohamad Isa Gabriela Di Lorenzo Garcia Scherer Michele Paula dos Santos Vitoria pereira Itaquy Talia Guimaraes dos Santos Julia Braga da Silveira Paulo Ricardo Martins Amanda Correa dos Santos Marcus Vinicius de Mello Pinto Janine Koepp Daiana Klein Weber Carissimi Joice Nedel Ott Carolina Goncalves Pinheiro nathalia Ken pereira Iketani Gabriela Feltez Bartira Ercília Pinheiro da costa Postgraduate Program in Medicine and Health Sciences of the Pontifical Catholic University of Rio Grande do Sul(PUC/RS) Porto AlegreBrazil Improvement of Higher Education Personnel Brazil(CAPES)Distrito FederalBrazil School of Medicine of the Pontifical Catholic University of Rio Grande do Sul(PUC/RS) Porto AlegreBrazil School of Health and Life Sciences of the Pontifical Catholic University of Rio Grande do Sul(PUC/RS) Porto AlegreBrazil Celulare Institute PetrópolisBrazil Undergraduate Nursing Course of the University of Santa Cruz do Sul(UNISC) Santa Cruz do SulBrazil Postgraduate Program in Health Sciences of the Faculty of Medicine of ABC(FMABC) Sao PauloBrazil Undergraduate Physiotherapy Course of the Vale do Salgado Faculty(FVS) CearáBrazil Physiotherapy of the Hospital Beneficiente Portuguesa of Belém ParáBrazil Rehabilitation Sciences of the Federal University of Health Sciences of Porto Alegre Porto AlegreBrazil Postgraduate Program in Medicine and Health Sciences School of Medicine of the Pontifical Catholic University of Rio Grande do Sul(PUC/RS)Porto AlegreBrazil

Atrial fibrillation is a cardiac arrhythmia of high prevalence in the population, especially in the elderly. Its main electrical characteristics are the interval between two successive irregular R waves, absence of P waves and presence of f waves between QRS complexes. The most common symptoms of atrial fibrillation are irregular palpitations associated with dyspnea, dizziness, feeling tired, fatigue and general malaise, but not all patients have any symptoms. The present report presents the history of an elderly patient who arrived at the hospital’s emergency department with irregular heart rhythm and palpitations. The patient’s symptoms, associated with the electrocardiogram results, indicated paroxysmal atrial fibrillation. Electrical cardioversion was performed, and after, cardiac ablation via the femoral vein at the hospital’s cardiology service. There were no complications during the procedure. As a routine imaging exam after ablation, control esophagogastroduodenoscopy was requested to verify that there was no formation of atrio-esophageal fistula developed by the invasive ablation procedure and electrocardiogram, which showed normal sinus rhythm. The patient remained in the cardiac intensive care unit for observation for 24 hours. After the electrical cardioversion and catheter ablation procedures, the patient improved his clinical picture of atrial fibrillation and was discharged after 24 hours of hospitalization. He received treatment to perform at home, to reduce acid reflux into the esophagus and to prevent thrombosis. He did not present pulmonary thromboembolism after hospital discharge. It is believed, therefore, that this form of treatment and management of paroxysmal atrial fibrillation is effective for the solution of the proposed problem and can also serve as a reference for other professionals within the cardiology service.

关键词： Atrial Fibrillation Electrical Cardioversion Catheter Ablation Hypertension Obstructive Sleep Apnea Syndrome

Protective Properties in Hymenaea martiana Hayne against Multi-drug-resistant Staphylococcus aureus

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Veterinary Science Research 2020年第2期2卷 16-23页

作者： Dielson da Silva Vieira Tânia Maria Sarmento da Silva Timothy AHackett Marianade Barros Weslen Fabrício Pires Teixeira Juliana Campos pereira Diniz Pedro HenriqueGorni Sanely Lourenço da costa Maria aparecida Scatamburlo Moreira MateusMatiuzzi da costa Francisco Leydson Formiga Feitosa São Paulo State University“Júlio de Mesquita Filho”(UNESP) School of Veterinary MedicineAraçatubaSao PauloBrazil Federal Rural University of Pernambuco(UFRPE) Department of Molecular SciencesRecifePernambucoBrazil Department of Biochemistry University of Nebraska-LincolnLincolnNE68508USA Federal University of Viçosa(UFV) Veterinary DepartmentViçosaMinas GeraisBrazil Federal University of Vale do São Francisco(UNIVASF) Department of Animal SciencePetrolinaPernambucoBrazil University of West Paulista(UNOESTE) Department of AgronomyPresidente PrudenteSao PauloBrazil Federal University of Goiás(UFG) Department of Veterinary MedicineGoiâniaGoiásBrazil

Antibiotic resistance represents a widespread problem in milk *** identification of compounds for a topically applied ointment used in mastitis therapy remains *** from the genus Hymenaea can be administered in cases of multi-drugresistant Staphylococcus aureus infection for ruminant species,but the protective properties are not well *** this research the aim is verify the protective effects of *** against *** infection in bovine mammary epithelial cell line(MAC-T)and to obtain an antioxidant profile evaluation in *** MAC-T cells were challenged with *** after being exposed to the extract of the *** in the protective *** the verification of the viability of the MAC-T cells,the MTT assay was performed,and was used dilutions of the plant extract,starting at 2.5%.The extract of *** was evaluated for antioxidant aspect in different dilutions by FRAP,ORAC and DPPH.A variety of flavonoids(quercetin,luteolin,etc.)have been identified as the main components by using mass spectrometry,reinforcing our in vitro findings that flavonoids,especially quercetin,have a medicinal profile capable of killing mastitis-causing *** excellent antioxidant pattern was observed in the 2.5%solution;however,membrane integrity in MAC-T cells was *** findings suggest low dilutions of *** extract has a desirable protective effect from *** *** in vitro studies can be gleaned upon for further in vivo studies.

关键词： Dairy animal Fitoterapic Hymenaea gender Cell activity Mastitis