检索结果-南通市图书馆

A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： natalia souza de godoy Manoel Sebastiao da Costa Lima-Junior Jose Angelo Lauletta Lindoso Vera Lucia Pereira-Chioccola Thelma Suely Okay Lucia Maria Almeida Braz Laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil Laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil Laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil Laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil Laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

decomposition and stabilization of organic matter in an old-growth tropical riparian forest:effects of soil properties and vegetation structure

引用

Forest Ecosystems 2021年第1期8卷 173-181页

作者： Pedro Henrique de godoy Fernandes Andréa Lúcia Teixeira de souza Marcel Okamoto Tanaka Renata Sebastiani Graduate Program in Environmental Sciences Federal University of São CarlosSão CarlosSPBrazil department of Environmental Sciences(DCAm) Federal University of São CarlosSão CarlosSPBrazil department of Nature Mathematicsand Education SciencesFederal University of São CarlosSão CarlosSPBrazil

Background:Nutrient cycling in tropical forests has a large importance for primary productivity,and decomposition of litterfall is a major process influencing nutrient balance in forest *** large-scale factors strongly influence decomposition patterns,small-scale factors can have major influences,especially in old-growth forests that have high structural complexity and strong plant-soil *** we evaluated the effects of forest structure and soil properties on decomposition rates and stabilization of soil organic matter using the Tea Bag Index(TBI)in an old-growth riparian forest in southeastern *** data sets were described separately using Principal Components Analysis(PCA).The main axes for each analysis,together with soil physical properties(clay content and soil moisture),were used to construct structural equations models that evaluated the different parameters of the TBI,decomposition rates and stabilization *** best model was selected using Akaike’s ***:Forest structure and soil physical and chemical properties presented large variation among plots within the studied *** content was strongly correlated with soil moisture and the first PCA axis of soil chemical properties,and model selection indicated that clay content was a better predictor than this *** rates presented a large variation among tea bags(0.009 and 0.098 g·g^(−1)·d−1)and were positively related with forest structure,as characterized by higher basal area,tree density and larger *** stabilization factor varied between 0.211–0.426 and was related to forest stratification and soil clay ***:The old-growth forest studied presented high heterogeneity in both forest structure and soil properties at small spatial scales,that influenced decomposition processes and probably contributed to small-scale variation in nutrient *** rates were only influenced by forest structure,whereas the stabilizat

关键词： Tea bag index Forest structure Carbon fixation Nutrient cycling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In vitro induced pluripotency from urine-derived cells in porcine

引用

World Journal of Stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan department of Surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil Embrapa Suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil department of Animal Sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 *** transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Relationship between Physical and Chemical Soil Attributes and Plant Species Diversity in Tropical Mountain Ecosystems from Brazil

引用

Journal of Mountain Science 2014年第4期11卷 875-883页

作者： Ferna de CARVALHO Edward Luis godoy Francy J. G. LISBOA Fatima Maria de souza MOREIRA Francisco Adriano de souza Ricardo Luis Louro BERBARA G Wilson FERNES Laboratory of Evolutionary Ecology and Biodiversity Department of Geral Biology Institute of Biology Sciences Federal University of Minas Gerais Belo Horizonte MG 30161-901 P.O. Box 486 Brazil department of Biology Microbiology and Biological Processes Department of Soil Science Federal University of LavrasLavras Minas Gerais 37200-000 P.O.Box 3037 Brazil department of Biological Sciences Ferderal University of S?o Jo?o Del Rei S?o Jo?o del-Rei Minas Gerais 36307-352Brazil Laboratory of Soil Biology Department of Soil Science Federal Rural University of Rio de Janeiro Seropédica Rio de Janeiro 23890-000 Brazil Embrapa Maize and Sorghum Center for Applied Biology Rod. MG 424 KM 45 Caixa Postal Sete Lagoas Minas Gerais 35701-970 P.O. Box 285 Brazil

Although the high diversity of plant species in the rupestrian fields has been primarily attributed to the existence of a set of distinct habitats, few studies support this assertion. The present study aimed to further investigate the relationship between physical and chemical attributes of soils with the diversity of plant species in this unique ecosystem. The rupestrian field is a unique vegetation formation that covers some of the southeastern Brazilian mountains in the transition of the Atlantic rain forest and the Cerrado(savanna). Different habitats occur according to soil characteristics(e.g., presence of rocks, sand, fertility, hydrology, etc.). These attributes ultimately influence the vegetation that is highly adapted to the harsh edaphic and climatic mountain conditions. Five distinct habitats were studied by us: rocky outcrops, peat bogs, sandy bogs, quartz gravel fields, and "cerrado"(savanna). A floristic survey indicated that four families are found at greater frequency: Poaceae, Asteraceae, Cyperaceae, and Leguminosae. The greatest diversity of plant species was found in the rocky outcrops habitat, followed by cerrado, peat bog, quartz gravel grassland, and sandy bogs, respectively. The main difference in the floristic composition among these habitats was related to the dominant species. Trachypogon spicatus(Poaceae) was the most dominant species in the rocky outcrops, Axonopus siccus(Poaceae) in the peat bogs, Lagenocarpus rigidus(Cyperaceae) in the sandy bogs, Schizachyrium tenerum(Poaceae) in the cerrado, while Vellozia sp. 8(Velloziaceae) dominated the vegetation in the quartz gravel grassland. This study demonstrated that physical and chemical soil properties strongly related the diversity of plant species occurring in the different habitats of rupestrian fields.

关键词：植物物种多样性山地生态系统土壤特性热带雨林属性化学物理巴西

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Integrating species and successional classes for wood production in a mixed forest restoration system in a neotropical region

引用

Journal of Forestry Research 2021年第6期32卷 2313-2321页

作者： Ananias Francisco Dias Junior Ana Lucia Martins Sodero Pincelli Ana Paula Camara da Silva Alison Moreira da Silva natalia Dias de souza Mario Tommasiello Filho Jose Otavio Brito Wood and Forestry Science department Federal University of EspIrito Santo(UFES)Av.Governador Lindemberg316JerOnimo Monteiro CityES 29550-000Brazil São Paulo State School of Technology(FATEC/SP) Av.DiacOno Jair de Oliveira651PiracicabaSP 13414-141Brazil Forest Products department Federal Rural University of Rio de Janeiro(UFRRJ)BR 465km 07SeropEdicaRJ 29890-000Brazil University of SAo Paulo “Luiz de Queiroz”College of Agriculture(USP/ESALQ)Av.PAdua Dias11PiracicabaSP 13418-900Brazil

As forests in neotropical regions,particularly in developing countries,are devastated,interventions to restore biodiversity and its ecological functions are *** producers have thus been encouraged to grow trees for wood production as an economic *** objective of this study was to quantify the increment of wood density of four forest species from different successional classes of a mixed system of restoration in a neotropical forest in *** discs were sampled at breast height and analyzed radially by X-ray densitometry to obtain apparent density and basic *** trees each of a species from the pioneer,early and late secondary successional stages and of the dominant species in the climax *** radial profiles indicated increasing density from the pith to bark of trunks,except for some variations due to wood defects and *** density was 0.576 g cm^(-3),0.655 g cm^(-3),0.706 g cm^(-3)and 0.775 g cm^(-3),respectively,for Peltophorum dubium,Schinus terebinthifolius,Cariniana estrellensis and Hymenaea *** profiles indicated higher amplitudes in the apparent densities for slow-growing species.X-ray densitometry generated parameters such as minimum,average and maximum densities,and radial density *** parameters are important for understanding the ecological functional role of successional classes of the Atlantic Forest from the Neotropical region.

关键词： X-ray densitometry Timber species of neotropical forest Successional classes of forest species Maintenance of forest restoration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Efficacy of Photogem（Hematoporphyrin derivative） as a Photoactivatable Larvicide against Aedes aegypti （Diptera： Culicidae） Larvae

引用

Journal of Life Sciences 2017年第2期11卷 74-81页

作者： Larissa Marila de souza natalia Mayumi Inada Sebastiao Pratavieira Juliano Jose Corbi Cristina Kurachi Vanderlei Salvador Bagnato Federal University of S6o Carlos PPG Biotec S6o Carlos 13565-905 Sao Paulo Brazil Sao Carlos Institute of Physics University of Sao Paulo Sao Carlos 13560-970 Sao Paulo Brazil Sao Carlos School of Engineering University of Sao Paulo Sao Carlos 13560-970 Sao Paulo Brazil

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Person-centered Care for Institutionalized Older Adults in the Context of the COVID-19 Pandemic in Brazil

引用

Journal of Geriatric Medicine 2022年第1期4卷 1-9页

作者： Marina Celly Martins Ribeiro de souza natalia de Cassia Horta Marco Aurélio Santos Pereira Júlia das Graças Rodrigues de Almeida Jasmine Yee Leonardo Ayres Cordeiro TaináRodrigues Gomide souza Pinto Constance Kartoz department of Public Health The College of New JerseyUSA department of Medicine Pontifical Catholic University of Minas GeraisBrazil department of Nursing Pontifical Catholic University of Minas GeraisBrazil department of Economics Pontifical Catholic University of Minas GeraisBrazil Business and Accounting department of the Federal University of Viçosa Brazil department of Nursing The College of New JerseyUSA

Objective:This study aims to discuss the caregiving practices developed by Long-term Care Facilities(LTCFs)during the COVID-19 pandemic and analyze the daily care practices in long-term institutions for older adults in Brazil,all in light of the PCC ***:This is a mixed methods study in which data were collected through interviews with managers from 10 *** qualitative discussion was carried out through the PCC framework divided into 5 categories:leisure,accommodation,food,hygiene and comfort,and clinical *** quantitative data collected were analyzed in a descriptive way,being discussed in the light of the ***:Analyzed LTCFs are unaware of our present difficulties in the implementation of PCC,with a greater presence of the traditional biomedical model being *** the vulnerabilities that the LTCFs present,PCC is an important alternative for LTCFs to promote the quality of life and autonomy of *** the vision of LTCFs as a last resort of care and investing in the quality of care is an urgent and essential imperative for dignified and comprehensive ***:This study highlights the need for a change in culture and understanding of the LTCFs not only as a place to provide healthcare,but also as a residents’home that fosters their autonomy,and feeling of ***,it is essential to ensure that healthcare teams in LTCFs know about PCC and that further studies investigate the impact on the costs of PCC for institutionalized older adults.

关键词： Pandemic COVID-19 Long-term care facilities Person-centered care Institutionalized older adults

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Apixaban, an orally available anticoagulant, inhibits SARS-CoV-2 replication and its major protease in a non-competitive way

引用

Journal of Molecular Cell Biology 2022年第6期14卷 68-71页

作者： Otávio Augusto Chaves Carolina Q.Sacramento natalia Fintelman-Rodrigues Jairo Ramos Temerozo Filipe Pereira-Dutra Daniella M.Mizurini Robson Q.Monteiro Leonardo Vazquez Patricia T.Bozza Hugo Caire Castro-Faria-Neto Thiago Moreno L.souza Laboratory of Immunopharmacology Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil National Institute for Science and Technology on Innovation on Neglected Diseases(INCT/IDN) Center for Technological Development in Health(CDTS)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil National Institute for Science and Technology on Neuroimmunomodulation(INCT/NIM) Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil Laboratory on Thymus Research Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil Institute of Medical Biochemistry Leopoldo de Meis Federal University of Rio de Janeiro(UFRJ)Rio de JaneiroRJ21941-590Brazil

dear Editor,The severe coronavirus disease 2019(COVID-19)is associated with ***,such as low-molecular-weight heparin,warfarin,thrombin inhibitors,and factor Xa(FXa)inhibitors,are thus recommended by the American Society of Hematology and National Institutes of Health for COVID-19 patients(Wenzler et al.,2020;Adam et al.,2021).Clinical trials with anticoagulants have shown the increased survival of critically ill COVID-19 patients under non-invasive and invasive ventilatory assistance(Wenzler et al.,2020;Adam et al.,2021),along with decreased consumption of platelets and clotting factors and a reduced risk of hemorrhage(Adam et al.,2021).Among the anti-clotting agents,early use of orally available FXa and thrombin inhibitors(Chowdhury et al.,2020;Rentsch et al.,2021)prevented high levels of D-dimer,which is the final product from the clotting/fibrinolysis cascade and is directly implicated with severe COVID-19(Rentsch et al.,2021).

关键词： al. Clinical heparin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

空间、环境和历史因素对热带森林树种周转的影响

引用

Journal of Plant Ecology 2021年第4期14卷 717-729页

作者： Alisson Borges Miranda Santos Vinicius Andrade Maia Cleber Rodrigo de souza Nathalle Cristine Alencar Fagundes Fernanda Moreira Gianasi Aurelio de Jesus Rodrigues Pais natalia de Aguiar-Campos Gabriela Gomes Pires Diego Teixeira Girardelli Jean Daniel Morel Mariana Caroline Moreira Morelli Felipe de Carvalho Araujo Rubens Manoel dos Santos department of Forestry Sciences Federal University of LavrasUniversity CampusLavrasBrazil Biological Sciences University of the State of Minas GeraisItuiutaba UnitItuiutabaBrazil department of Biology Federal University of LavrasUniversity CampusLavrasBrazil Faculty of Environmental Engineering and Natural Resources Zambeze UniversityChimoioMozambique

本文通过回答以下问题来分析空间距离、当前和过去的环境差异及这几个因素对树木群落分类和系统发育转换的影响:(i)树木群落分类和系统发育转换是否与空间距离通过环境差异产生的间接影响相关?(ii)树木群落分类和系统发育转换是否随古气... 详细信息

本文通过回答以下问题来分析空间距离、当前和过去的环境差异及这几个因素对树木群落分类和系统发育转换的影响:(i)树木群落分类和系统发育转换是否与空间距离通过环境差异产生的间接影响相关?(ii)树木群落分类和系统发育转换是否随古气候(末次盛冰期和中全新世)的变化而变化?本文对巴西的14个大西洋雨林样点(采样面积20.4 ha)83个科615个种进行了研究,获得了当前、中全新世和末次盛冰期的地块地理坐标、土壤变量和样点生物气候变量。我们使用基于距离的结构方程模型(SEM)来(i)检测空间距离和环境差异的直接影响,以及(ii)检测空间距离通过环境差异对分类(Bray-Curtis距离)和系统发育转换(Comdist和Comdistnt距离)的间接影响。研究结果表明,空间距离通过环境差异对分类和系统发育转换的间接影响较弱。基于生态位中性的历史过程带来的直接影响驱动了树木群落的转换。因此,我们推断古气候(历史过程)促进了产生当前植物区系分支的选择,而空间距离(中性的过程)限制了物种从区域种库向外扩散的范围,环境条件(基于生态位的过程)则在本地选择能够持续生存的分类群。

关键词：聚类过程系统发育群落环境过滤中性群落系统发育转换生态位古气候效应

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intestinal Microbiota, Obesity and Insulin Resistance—What Are the Relationships?

引用

Health 2018年第4期10卷 365-373页

作者： Lígia Maria Cordeiro Misurini Nicole do Prado Olbrzymek do Prado Olbrzymek Cicera Aparecida Mendes Letícia godoy Dias Sanderson Renata Florenzano Flores Luiz Henrique Corrêa Portari Portari Luciana Corrêa de souza Rodrigues Diana Aristotelis Rocha de Sá Larissa Bianca Paiva Cunha de Sá Alberto Krayyem Arbex Endocrinology Division IPEMED Medical School Sao Paulo Brazil Diabetology department Malteser Krankenhaus St. Franziskus Hospital Flensburg Germany Harvard T. H. Chan School of Public Health Boston MA USA

Obesity is a public health problem and its prevalence increases every year, resulting from a complex interaction between genetic, metabolic and environmental factors, as well as cultural influences. The Intestinal Microbiota is composed of trillions of microorganisms by forming a symbiotic relationship with the host and helping the absorption of various nutrients, increasing the extraction of components of the diet, the lipogenesis and intestinal permeability. Studies show that there is difference between the composition of the intestinal microbiota of an obese person and a healthy one. The purpose of this article is to review the literature on the relationship between the Intestinal Microbiota, Obesity and Insulin Resistance and also illustrate some studies on the subject.

关键词： Obesity Intestinal Microbiota Insulin Resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：