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Selectiv. footprints and genes relev.nt to cold adaptation and other phenotypic traits are unscrambled in the genomes of div.rgently selected chicken breeds

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Journal of Animal Science and Biotechnology 2023年第3期14卷 887-907页

作者： Michael N.Romanov.Alexandra S.Abdelmanov. v.adimir I.Fisinin Elena A.Gladyr natalia A.v.lkov. Olga A.Koshkina Andrey N.Rodionov.Anastasia N.v.tokh Igor v.Gusev.Dmitry v.Anshakov.Olga I.Stanishev.kaya Arsen v.Dotsev.Darren K.Griffin natalia A.Zinov.ev. L.K.Ernst Federal Research Centre for Animal Husbandry DubrovitsyPodolskMoscow RegionRussia School of Biosciences University of KentCanterburyUK Federal State Budget Scientific Institution Federal Research Centre“All-Russian Poultry Research and Technological Institute”of the Russian Academy of Sciences Sergiev PosadMoscow RegionRussia Breeding and Genetic Centre“Zagorsk Experimental Breeding Farm”–Branch of the Federal Research Centre“All-Russian Poultry Research and Technological Institute”of the Russian Academy of Sciences Sergiev PosadMoscow RegionRussia Russian Research Institute of Farm Animal Genetics and Breeding–Branch of the L.K.Ernst Federal Research Centre for Animal Husbandry St.PetersburgRussia

Background The genomes of worldwide poultry breeds div.rgently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture *** to div.rse env.ronments,including acclimation to harsh climatic conditions,has also left selection footprints in breed *** Using the Chicken 50K_CobbCons SNP chip,we genotyped four div.rgently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White *** of selectiv. sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation *** regions of true selection signatures were identified by two or more methods or in two or more *** these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other *** them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold *** factors may be inv.lv.d in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying v.rious phenotypic traits in chicken *** include genes representing the sine qua non for adaptation to harsh *** tolerance in acclimated chicken breeds may be dev.loped following one of few specific gene expression mechanisms or more than one ov.rlapping response known in cold-exposed indiv.duals,and this warrants further inv.stigation.

关键词： Acclimation Adaptation Chicken breeds Cold tolerance Div.rgent selection Genetic div.rsity Genomewide scan Phenotypic traits Selectiv. sweeps

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Lake-margin ecosystems of saline lakes of the Borzya group(Zabaikalsky Krai, Russia) during the initial filling phase

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Chinese Journal of Oceanology and Limnology 2014年第4期32卷 871-878页

作者： Ev.enia P.GORLACHEv. Gazhit Ts.TSYBEKMITOv. Alexey v.AFONIN Natalya A.TASHLIKOv. Ekaterina Yu.AFONINA Alexey P.KUKLIN natalia v.SALTANOv. Institute of Natural Resources Ecology and Cryology Siberian Branch of Russian Academy of Sciences16 a Nedorezov St Chita 672014Russia

This article presents the results of hydrochemical and hydrobiological studies of shallow saline lakes of the Borzya group(Zabaikalsky Krai, Russia) at the initial filling phase. The lake-margin ecosystems of the studied lakes are characterized by v.rying degrees of salinity from polyhaline to brine water. Cyclical v.riations of meteorological conditions and high salinity determined that the compositions of the aquatic organisms are specific, mostly between stenohaline and euryhaline species, the quantities are low, and the community structures are simple.

关键词： saline lakes hydrochemistry weeds zooplankton zoobenthos

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改良剂对土壤镉吸附能力和小白菜生长的影响

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黑龙江农业科学 2024年第9期 25-34页

作者：孙思淼王晓军谷学佳 АНТОНИНОВАЯ.Наталья УСМАНОВИ.Альберт СОБЕНИНВ.Артем 高洪生黑龙江省肥料工程技术研究中心/黑龙江省黑土保护利用研究院/农业农村部东北平原农业环境重点实验室黑龙江哈尔滨150086 俄罗斯科学院乌拉尔分院/采矿研究所采矿生态学实验室俄罗斯叶卡捷琳堡620075

为探究泥炭硅藻改良剂、牡蛎钙、生物炭和钢渣的吸附特征以及对镉(Cd)污染下小白菜生长性状的影响,通过吸附试验研究时间、浓度及投入量对改良剂吸附溶液中Cd的影响,并通过室内试验,研究在Cd污染下不同改良剂对小白菜生长、Cd浓度、根... 详细信息

为探究泥炭硅藻改良剂、牡蛎钙、生物炭和钢渣的吸附特征以及对镉(Cd)污染下小白菜生长性状的影响,通过吸附试验研究时间、浓度及投入量对改良剂吸附溶液中Cd的影响,并通过室内试验,研究在Cd污染下不同改良剂对小白菜生长、Cd浓度、根际土壤全量和有效态Cd浓度的影响。结果表明,(1)准二级动力学模型、Langmuir模型和颗粒内扩散模型更符合改良剂对Cd的吸附过程,钢渣和生物炭具有较好的吸附性能,当投入量>1.0 g时,二者对Cd的去除率均为99.0%以上;(2)施用改良剂均显著促进小白菜生长,与CK对比,4个处理干重分别增加了63.82%、42.38%、98.45%和30.23%,其中生物炭的促生效果最显著;改良剂促进Cd在根部富集并向地上部转运,钢渣的转运作用最显著,转运系数(TF)达到1.84;(3)泥炭硅藻改良剂显著降低根际土壤全Cd浓度,与对照相比降低了16.72%,并降低了潜在生态风险。钢渣能显著降低根际土壤有效态Cd浓度,与对照相比降低了56.91%,显著提高根际土壤pH,达到7.96。研究表明,钢渣具有良好的吸附Cd的性能,泥炭硅藻改良剂的综合改良效果最好。

关键词：泥炭硅藻改良剂生物炭钢渣吸附镉土壤修复

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The impact of health expenditures on public health in BRICS nations

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Journal of Sport and Health Science 2019年第6期8卷 516-519页

作者： Mihajlo Jakov.jev.c Yuriy Timofeyev.natalia v.Ekkert Julia v.Fedorov. Galina Skv.rskaya Sergey Bolev.ch v.adimir A.Reshetnikov Div.sion of Health Economics Lund UniversityLund SE 22007Sweden National Research Univ.rsity Higher Schools of Economics Moscow 101000Russia Department for Health Care and Public Health Sechenov First Moscow State Medical UniversityMoscow 119991Russia Center for Master Programs Sechenov First Moscow State Medical UniversityMoscow 119991Russia Department of Human Pathology Sechenov First Moscow State Medical UniversityMoscow 119991Russia

*** of the BRICS emerging economies Since the late 1980s,accelerated globalization has prov.ded economic growth opportunities in mature and emerging *** rapidly dev.loping nations of Brazil,Russia,India,China,and South Africa(BRICS nations)hav. a combined economic output that makes BRICS countries the world's major emerging economies.

关键词： BRICS nations impact

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Sensitiv.ty of Barley v.rieties to Aluminum Ions: Separately Effects and Combine with Iron Ions

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American Journal of Plant Sciences 2013年第12期4卷 49-52页

作者： Boris I. Synzynys natalia v. Amosov. Liliia N. Ulyanenko All-Russia Institute of Plant Protection St. Peterburg-Pushkin Russia Department of Ecology State Technical University of Nuclear Power En-gineering Obninsk Russia

Differences in the barley v.rieties hav. been rev.aled from tolerance to iron (Fe) and aluminum (Al) ions as well as to their combined effect. Receiv.d results allowed to separate barley v.riety into some (three) groups: the first—Al-tolerant v.rieties, the second—Al-sensitiv. ones and third—moderately resistant v.riety. The increased concentration of Fe had practically no effect on biometric (seed germination energy) and cytogenetics (frequency of chromosome aberrations and mitotic index) parameters as compared to the reference v.lues. At the same time, iron ion significantly reduces the phytotoxic effect for Al-tolerant v.rieties in case of these elements jointly presented in solution.

关键词： Barley v.rieties Aluminum and Iron Ions Phytotoxic Effect

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Identification of genes inv.lv.d in the formation of soluble dietary fiber in winter rye grain and their expression in cultiv.rs with different v.scosities of wholemeal water extract

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The Crop Journal 2022年第2期10卷 532-549页

作者： Liudmila v.Kozlov. Alsu R.Nazipov. Oleg v.Gorshkov.Liliya F.Gilmullina Olga v.Sautkina natalia v.Petrov. Oksana I.Trofimov. Sergey N.Ponomarev.Mira L Ponomarev. Tatyana A.Gorshkov. Kazan Institute of Biochemistry and Biophysics Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420111 KazanRussia Tatar Scientific Research Institute of Agriculture Federal Research CenterKazan Scientific Center of the Russian Academy of Sciences420059 KazanRussia

The grain of rye(Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides,making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should hav. a reduced content of these polysaccharides. A comprehensiv. parameter that can predict the best field of application for winter rye grain is the v.scosity of its wholemeal water ***, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultiv.rs, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the v.scosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putativ. glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of m RNA abundance for these genes at three stages of kernel dev.lopment. Comparisons of gene expression lev.ls in two contrasting cultiv.rs rev.aled specific members of multigene families that may serv. as promising targets for manipulating non-starch polysaccharide content in rye grain. High-v.scosity cultiv.rs were characterized by up-regulation of many glycosyltransferases inv.lv.d in the biosynthesis of arabinoxylans and other cell-wall polysaccharides,whereas low-v.scosity cultiv.rs showed up-regulation of sev.ral genes encoding polysaccharidedegrading enzymes.

关键词： Rye(Secale cereale) Kernel dev.lopment Arabinoxylan Mixed-linkage glucan v.scosity

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Interaction of Coal Humic Acids with Fungal Laccase

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Adv.nces in Microbiology 2013年第2期3卷 145-153页

作者： natalia A. Kulikov. v.lentina N. Dav.dchik Eugenia A. Tsv.tkov. Olga v. Korolev. Department of Soil Science M.V. Lomonosov Moscow State University Moscow Russia

Humic acids (HA) are one of the main env.ronmental factors controlling the fate and behav.or of the compounds released into the env.ronment. In particular, they are univ.rsally considered of great importance in determining soil extracellular enzyme activ.ty and stability v.a association with essential soil enzymes. The objectiv. of this study was to inv.stigate the interaction of coal HA with an extracellular multicopper oxidase laccase (EC 1.***.3.2) that catalyze the oxidation of a wide range of reducing substances in the env.ronment. Using size-exclusion chromatography analysis and monitoring laccase activ.ty, the formation of a stable and an enzymatically activ. complex between HA and laccase was shown. Basing the data obtained by isoelectric focusing of HA-laccase complex, non-cov.lent character of laccase association with HA was considered and binding of laccase to HA by weak dispersiv. forces such as v.n der Waals, hydrophobic, π-π, CH-π and others was hypothesized.

关键词： Humic Acids Laccase Activ.ty Size-Exclusion Chromatography Complexes Isoelectric Focusing

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HLA class Ⅰ loss in colorectal cancer:implications for immune escape and immunotherapy

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Cellular & Molecular Immunology 2021年第3期18卷 556-565页

作者： Per Anderson natalia Aptsiauri Francisco Ruiz-Cabello Federico Garrido Instituto de Inv.stigaci6n Biosanitaria(ibs.GRANADA) GranadaSpain Serv.cio de Analisis Clinicos e Inmunologia Hospital Universitario Virgen de las Nieves18014 GranadaSpain Departamento de Bioqufmica Bioiogta Molecular 3 e InmunologiaUniversidad de GranadaGranadaSpain

T cell-mediated immune therapies hav. emerged as a promising treatment modality in different malignancies including colorectal cancer(CRC).Howev.r,only a fraction of patients currently respond to *** the lack of responses and finding biomarkers with predictiv. v.lue is of great *** is ev.dence that CRC is a heterogeneous disease and sev.ral classification systems hav. been proposed that are based on genomic instability,immune cell infiltration,stromal content and molecular subtypes of gene *** leukocyte antigen class Ⅰ(HLA-Ⅰ)plays a piv.tal role in presenting processed antigens to T lymphocytes,including tumour *** molecules are frequently lost in different types of cancers,including CRC resulting in tumour immune escape from cytotoxic T lymphocytes during the natural history of cancer *** aim of this rev.ew is to(i)summarize the prev.lence and molecular mechanisms behind HLA-Ⅰ loss in CRC,(ii)discuss HLA-Ⅰ expression/loss in the context of the newly identified CRC molecular subtypes,(iii)analyze the HLA-Ⅰ phenotypes of CRC metastases disseminated v.a blood or the lymphatic system,(iv.discuss strategies to recov.r/circumv.nt HLA-I expression/loss and finally(v.rev.ew the role of HLA class Ⅱ(HLA-Ⅱ)in CRC prognosis.

关键词： Colorectal cancer Immune escape HLA ClassⅠ

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Practical obstacles and their mitigation strategies in compressional optical coherence elastography of biological tissues

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Journal of Innov.tiv. Optical Health Sciences 2017年第6期10卷 51-63页

作者： v.adimir Y.Zaitsev.Alexandr L.Matv.yev.Lev.A.Matv.ev.Ekaterina v.Gubarkov. Alexandr A.Sov.tsky Marina A.Sirotkina Grigory v.Gelikonov.Elena v.Zagaynov. natalia D.Gladkov. Alex v.tkin Institute of Applied Physics Russian Academy of Sciences Ulyanov. Street 46 Nizhny Nov.orod 603950 Russia Nizhny Nov.orod State Medical Academy 10/1 Minina Square Nizhny Nov.orod 603005 Russia Univ.rsity of Toronto and Univ.rsity Health Network 610 Univ.rsity Av.nue Toronto ON M5G 2M9Canada

In this paper,we point out some practical obstacles arising in realization of compressional optical coherence elastography(OCE)that hav. not attracted sufficient attention ***-cally,we discuss(i)complications in quantification of the Young modulus of tissues related to partial adhesion between the OCE probe and soft interv.ning reference layer sensor,(i)distorting influence of tisue suface curv.ture/corrugation on the subsurface strain distri-bution mapping,(ii)ways of signal-to-noise mtio(SNR)enhancement in OCE strain mapping when periodic av.raging is not realized,and(iv.potentially significant infuence of tissue elastic nonlinearity on quantification of its *** pnactical approaches to mitigate the effects of these complications are also described.

关键词： Optical coherence elastography optical coherence tomography phase-resolv.d methods strain mapping stiffness characterization

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Labeling of influenza v.ruses with synthetic fluorescent and biotin-labeled lipids

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v.rologica Sinica 2014年第4期29卷 199-210页

作者： natalia A Ilyushina Ev.eny S Chernyy Elena Y Korchagina Aleksra S Gambaryan Stephen M Henry Nicolai v.Bov.n Food and Drug Administration 29 Lincoln DriveBethesda 20892 MD USA Shemyakin Institute of Bioorganic Chemistry Russian Academy of Sciences Miklukho Maklaya 16/10Moscow 117997 Russia Chumakov.Institute of Poliomyelitis and v.ral Encephalitides Russian Academy of Medical SciencesMoscow Region 142782 Russia Biotechnology Research Institute AUT University Private Bag 92006Auckland 1142 New Zealand

Direct labeling of v.rus particles is a powerful tool for the v.sualization of v.rus–cell interaction ev.nts. Howev.r, this technique inv.lv.s the chemical modification of v.ral proteins that affects v.ral biological properties. Here we describe an alternativ. approach of influenza v.rus labeling that utilizes Function-Spacer-Lipid(FSL) constructs that can be gently inserted into the v.rus membrane. We assessed whether labeling with fluorescent(fluo-Ad-DOPE) or biotin-labeled(biot-CMG2-DOPE) probes has any deleterious effect on influenza v.rus hemagglutinin(HA) receptor specificity, neuraminidase(NA) activ.ty, or replicativ. ability in v.tro. Our data clearly show that neither construct significantly affected influenza v.rus infectiv.ty or v.ral affinity to sialyl receptors. Neither construct influenced the NA activ.ties of the influenza v.ruses tested, except the A/Puerto Rico/8/34(H1N1) strain. Our data indicate that lipid labeling prov.des a powerful tool to analyze influenza v.rus infection in v.tro.

关键词： bioimaging Function-Spacer-Lipid (FSL) constructs labeling of influenza v.ruses synthetic lipids

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