Interaction of Coal Humic Acids with Fungal Laccase

作     者：Natalia A. Kulikova Valentina N. Davidchik Eugenia A. Tsvetkova Olga V. Koroleva 

作者机构：Department of Soil Science M.V. Lomonosov Moscow State University Moscow Russia 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2013年第3卷第2期

页      面：145-153页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Humic Acids Laccase Activity Size-Exclusion Chromatography Complexes Isoelectric Focusing 

摘      要：Humic acids (HA) are one of the main environmental factors controlling the fate and behavior of the compounds released into the environment. In particular, they are universally considered of great importance in determining soil extracellular enzyme activity and stability via association with essential soil enzymes. The objective of this study was to investigate the interaction of coal HA with an extracellular multicopper oxidase laccase (EC 1.***.3.2) that catalyze the oxidation of a wide range of reducing substances in the environment. Using size-exclusion chromatography analysis and monitoring laccase activity, the formation of a stable and an enzymatically active complex between HA and laccase was shown. Basing the data obtained by isoelectric focusing of HA-laccase complex, non-covalent character of laccase association with HA was considered and binding of laccase to HA by weak dispersive forces such as van der Waals, hydrophobic, π-π, CH-π and others was hypothesized.