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Characteristics of Amorphous Mn_3AlC Phases Formed by Mechanical Milling

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Journal of Materials Science & Technology 1997年第2期13卷 149-153页

作者： Fuxing YIn nanju GU (Hebei Institute of Technology, Tianin 300132, China)T.Shigematsu n.nakanishi(Faculty of Science, Konan University Kobe 658, Japan)Y.Osawa A.Sato(national Research Institute of Metals, Tsukuba 305, Japan) Hebei Institute of Technology Tianjin 300132 China Faculty of Science Konan University Kobe 658 Japan national Research Institute of Metals Tsukuba 305 Japan

Amorphization of Mn carbides by rapid quenching method has been proved to be *** mechanical milling in the present work, amorphous phases have been formed in the perovskitetype Mn3AlC compound. The transformation process from crystalline to amorphous phase was found to be milling time controlled, and 10 h is a critical period for the macroscopically single amorphous phase to form. It has been clarified that the redistribution of C atoms is an instinctive factor for amorphous phase to nucleate, and the atomic configuration in the amorphous phase is changed much in longer milling that different crystallizing behaviours have been caused. With X-ray diffraction and differential scanning calorimetry (DSC) results, an atomic configuration transition model is considered for the structures of the amorphous phases changed during milling.

关键词： Mn Ta

收缩压变异性与2型糖尿病患者糖尿病肾病及动脉粥样硬化相关

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中华高血压杂志 2012年第6期20卷 599-599页

作者： Okada H Fukui M Tanaka M Inada S Mineoka Y nakanishi n Senmaru T Sakabe K Ushigome E Asano M Yamazaki M Hasegawa G nakamura n 刘莉叶鹏

近期许多研究显示收缩压变异性对心血管疾病发展有影响。该研究旨在探讨收缩压变异性和2型糖尿病患者糖尿病肾病和动脉粥样硬化严重程度之间的关系。

关键词：收缩压变异性动脉粥样硬化糖尿病肾病 2型糖尿病患者疾病发展心血管

Approaches towards the core pentasaccharide in n-linked glycans

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Chinese Chemical Letters 2018年第1期29卷 35-39页

作者： ning Wang Sheng-Tao Li Tian-Tian Lu Hideki nakanishi Xiao-Dong Gao Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education School of Biotechnology Jiangnan University

All eukaryotic cells share a common core pentasaccharide, Man3GlcnAc2 （M3）, which is found in both dolichol-linked oligosaccharides （DLOs） and n-linked glycoproteins. A pentasaccharide M3 can be elongated and elaborated by various glycosyltransferases and is thus considered as the key intermediate in the in vitro synthetic pathway of n-glycans. In this review, we describe the progresses in the preparation of M3 by chemical and chemoenzymatic methods. We first draw a brief schematic view of the typical n-glycan biological pathway and then focus on the requirements and challenges in obtaining core pentasaccharides. Representative methods and recent reported findings, especially research progress in chemoenzymatic synthesis, are highlighted. In addition, the opportunities in exploiting novel methods for constructing n-glycans, as well as their applications, are discussed.

关键词： n-GlycanPentasaccharideChemoenzymatic synthesisAlg proteinsMannosyltransferase

Molecular Basis of neuroimmune Interaction in an In Vitro Coculture Approach

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Cellular & Molecular Immunology 2008年第4期5卷 249-259页

作者： Mamoru nakanishi Tadahide Furuno School of Pharmacy Aichi Gakuin University 1-100 Kusumoto-choChikusa-kuNagoya 464-8650Japan

A team of researchers from nagoya, Tokyo and Hamilton developed a unique technique for studying neuroimmune interaction with confocal laser scanning fluorescence microscopy several years ago. It relies on guiding immune and nerve cell interaction by creating an adhesive environment using an in vitro coculture dish. With their technique, they are able to study details of the mechanism of how nerve cells communicate with immune cells （mast cells and T lymphocytes） and vice versa. They showed that nerve-mast cell communication could occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating v/a nK-1 receptors, was a soluble factor of this communication. In addition, recently, they showed that ATP which was released from activated mast cells mediated the activation of nerve cells. Further, with their technique, nagoya＇s group was able to study details of the molecular mechanism of nerve-mast cell interaction. n-cadherin and CADM1 （cell adhesion molecule 1） appeared to mediate attachment and promoted the communication between mast cells and nerves predominantly. It would lead to new therapeutic modalities for diseases based on neuroimmune interaction such as neurogenic inflammation, intestinal bowel diseases, asthma, and autoimmune disorders. Cellular ＆ Molecular Immunology. 2008;5（4）：249-259.

关键词： neuro-immunology coculture confocal microscopy soluble factor adhesion molecule

Transcatheter Closure *** Ligation in Preterm Infants with Patent Ductus Arteriosus:A Systematic Review and Meta-Analysis

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Congenital Heart Disease 2023年第2期18卷 245-265页

作者： Rohan Suresh Daniel Georgia K.Schmidt Hayato nakanishi Karen Smayra Mariah n.Mascara Dilip K.Vankayalapati Reem H.Matar Christian A.Than George Shiakos Ioannis Tzanavaros St George’s University of LondonLondonUK University of nicosia Medical School University of NicosiaNicosia2417Cyprus Division of Gastroenterology and Hepatology Mayo ClinicRochester55905USA School of Biomedical Sciences The University of QueenslandSt LuciaBrisbane4072Australia Cardiac Innovation Center of Apollonion Private Hospital NicosiaCyprus

Background:Persistent patent ductus arteriosus(pPDA)is a common condition in preterm *** metaanalysis aimed to assess the safety and efficacy of transcatheter closure(TC)when compared to surgical ligation(SL)in preterm infants with ***:A literature search of Ovid Cochrane Library,Medline,Embase,Epub,Scopus,PMC Preprints,and *** was conducted from inception to May 06,*** studies reported infants diagnosed with pPDA born at≤2000 g birth weight or at≤37 weeks’who underwent TC or SL as *** review was registered in PROSPERO(CRD42022325944).Results:From 97 studies screened,8 studies met the eligibility criteria,with a total of 756 preterm infants undergoing either TC(n=366)or SL(n=390).Compared to TC,SL had higher mortality rates(OR=0.32,95%CI:0.16,0.66,I^(2)=0%).no difference was seen in post-procedural complication rate(OR=0.90,95%CI:0.18,4.44,I^(2)=79%),mean duration of post-procedural mechanical ventilation(MD=−2.21 days,95%CI:−4.88,0.47,I^(2)=60%),hospital stay length(MD=−8.30 days,95%CI:−17.03,0.44,I^(2)=0%)or neonatal intensive care unit stay length(MD=−3.50 days,95%CI:−10.27,3.27,I^(2)=0%).Conclusion:Our meta-analysis demonstrated TC as a viable alternative option in managing preterm infants with pPDA in the context of *** the promising trends demonstrated in this meta-analysis,further studies with larger sample size and controlled baseline characteristics are needed to evaluate the safety and efficacy of TC and SL for preterm infants with pPDA.

关键词： Patent ductus arteriosus transcatheter surgery preterm infants systematic review

nMR Studies on the Interactions between Proteins and Antigen Peptides in MHC Class I Protein Complex of Immune System

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NMR Studies on the Interactions between Proteins and Antigen...

3-(2-咪唑)硫甲基头孢菌素类的合成和构效关系

第十三届全国波谱学学术会议

作者： Hiroshi nakanishi Biological Information Research Center National Institute of Advanced Science and IndustryTsukuba Central 61-1HigashiTsukubaIbaraki 305-8566Japan

＜正＞A major histocompatibility complex（MHC）class Ⅰ protein consists of heavy chain which has α-1,2,3 domains and light chain（β2,microgiobulin,β2 m）.An antigen peptide binds to a peptide binding groove in the... 详细信息

关键词： nMR interaction between peptide and protein MHC Class I protein Antigen peptides line broadening Transfer-nOE 15n and D enriched protein β2-microglobulin

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国外医药（抗生素分册） 1990年第5期11卷 336-338页

作者： E.nakanishi 李勇然

化学 3-取代基-2-巯基咪唑化物按照从前报道过的工艺路线制备,见图1。 n-烷基甘氨酸衍生物(Ⅰ),甲酰化和环化成咪唑化合物(Ⅲ),再转化成化合物(Ⅳ)和(Ⅴ)。接着,咪唑衍生物(Ⅲ-Ⅴ)与CTX以通常的方法反应,得到化合物Ⅵ(图2)。制备的这类... 详细信息

化学 3-取代基-2-巯基咪唑化物按照从前报道过的工艺路线制备,见图1。 n-烷基甘氨酸衍生物(Ⅰ),甲酰化和环化成咪唑化合物(Ⅲ),再转化成化合物(Ⅳ)和(Ⅴ)。接着,咪唑衍生物(Ⅲ-Ⅴ)与CTX以通常的方法反应,得到化合物Ⅵ(图2)。制备的这类头孢菌素的结构见表一。抗微生物活性表二比较了这一系列化合物的体外抗菌杆菌包括缘脓杆菌显示了优越的活性。此外,试验结果也说明如下的构效关系。 1.化合物3和4由于结构上的羧基少于化合物1,导致抗菌活性相似或稍低于化合物1,对绿脓杆菌的活性明显减弱。 2.化合物2抗菌活性显示,唑咪环的活力。其中有一些化合物对不同种类的革兰氏阴性细菌显示高度活性。在与CTX和CMX比较时。

关键词：头孢菌素类合成化学结构

Homology Modeling of Human Alpha-Glucosidase Catalytic Domains and SAR Study of Salacinol Derivatives

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Open Journal of Medicinal Chemistry 2012年第3期2卷 50-60页

作者： Shinya nakamura Kazunori Takahira Genzoh Tanabe Osamu Muraoka Isao nakanishi Department of Pharmaceutical Sciences Kinki University Kinki Japan

Maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) belong to human intestinal alpha-glucosidase and their n-terminal side catalytic domains are called ntMGAM and ntSI, and their C-terminal side catalytic domains are called CtMGAM and CtSI. As an antidiabetic, alpha-glucosidase inhibitor is required to bind to all of these domains to inhibit disaccharides hydrolysis. Salacinol and kotalanol isolated from Salacia reticulata are novel seed compounds for al-pha-glucosidase inhibitor. Even though the complex structures of ntMGAM or ntSI have been determined experimen-tally, those of CtMGAM and CtSI have not been revealed. Thus, homology modeling for CtMGAM and CtSI has been performed to predict the binding mode of salacinol and its derivatives for each domain. The binding affinities for these compounds were also calculated to explain the experimental structure-activity relationships (SARs). After a docking study of the derivatives to each catalytic domain, the MM/PBSA method has been applied to predict the binding affinities. The predicted binding affinities were almost consistent with the experimental SARs. The comparison of the complex structures and binding affinities provided insights for designing novel compounds, which inhibit all catalytic domains.

关键词： Homology Modeling Docking Study MM/PBSA Alpha-Glucosidase Salacinol Kotalanol

Intracellular Localization of ABC Transporter TAPL Differs between Transient and Stable Expression

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CellBio 2014年第2期3卷 50-59页

作者： Yu Motohashi Ayako Ohashi-Kobayashi Mayumi nakanishi-Matsui Yasuyuki Fujimoto Masatomo Maeda Department of Biochemistry School of Pharmacy Iwate Medical University Shiwa-Gun Japan Department of Immunology School of Pharmacy Iwate Medical University Shiwa-Gun Japan Department of Molecular Biology School of Pharmacy Iwate Medical University Shiwa-Gun Japan Laboratory of Biochemistry and Molecular Biology Graduate School of Pharmaceutical Sciences Osaka University Suita Japan

Transporter associated with antigen processing (TAP)-like (TAPL;ABCB9) is a half-type ABC transporter with sequence similarity to TAP1 and TAP2 that function in the ER membrane. To determine the cellular localization, TAPL and truncated forms of it were tagged with GFP at their car-boxyl termini. Intracellular localization of these fusion proteins was compared between transient and stable expression in CHO-K1 cells. When they were expressed transiently, the fluorescence of the fusion proteins was detected on the intracellular membrane, mainly in the ER, and all the fusion proteins, i.e., TAPL(M1 -A766)-GFP, TAPL(M1-S275)-GFP, TAPL(M1-K182 )-GFP, TAPL(M 1-R141)-GFP and TAPL(M1-G75), were co-localized with an ER marker, PDI. However, the fluorescence of all of them except for TAPL(M1-G 75)-GFP and TAPL(M1-S275)-GFP overlapped with a lysosome marker, cathepsin D, upon stable expression. Lysosomal localization was similarly observed with TAPL(M1- A766)-DsRed, which was stably expressed. These results suggest that TAPL is sorted to the lysosomal membrane when expressed stably in CHO-K1 cells. Furthermore, the lysosomal targeting signal may comprise the n-terminal four transmembrane helices since the n-terminal two transmembrane helices may not be enough to function as such a signal.

关键词： ABCB9 Lysosome Membrane Localization Stable Expression TAPL Transfection