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New Technique to Produce Large Amount of Flat Silk by Biospinning

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Agricultural Sciences 2014年第14期5卷 1483-1490页

作者： Laura Beatriz Garay Andrea Nembri Alana Lucia Oro Veronica Aureliana Fassina Claudia Regina das Neves Saez Alex Sandro Chiarello naiara climas pereira Graziele Milani Pessini Roxelle Ethienne Ferreira Munhoz Maria Aparecida Fernandez Seda y Fibras S.R.L HernandariasParaguay Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil

Beyond the production of silk thread, there are several studies showing that the silk is a great biomaterial for surgical sutures and grafts. This paper shows a new technique to produce silk thread changing the natural cycle of silk production, which is the production of cocoons. This new method has the purpose of producing a silk fabric free of impurities, through flat surfaces. Six different surfaces were tested: Glass, Formica Surface, Steel and Zinc Sheets, Cotton tissue and Burlap Bag. The first five surfaces had not presented enough larvae alive for statistical analysis, because there were several damages in silkworms larvae that resulted in mortality and low silk production. On the other hand, the burlap bag surface presented good results for web construction by biospinning and its use was indicated for silk industry focused on biomaterials. The present study suggested the potential of naturally biospun web, using Bombyx mori, to develop a new technique to produce silk thread matrices that will have several applications at the industry and production of biomedical materials.

关键词： Silkworm Bombyx mori L. Silk Thread Biomaterial

Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

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Open Journal of Genetics 2014年第5期4卷 370-377页

作者： Claudia Regina das Neves Saez Roxelle Ethienne Ferreira Munhoz naiara climas pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto naiara climas pereira Claudia Regina das Neves Saez Rafaela Bespalhuk Veronica Aureliana Fassina Graziele Milani Pessini Mayarha Patricia Dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole

Terrain radiometric calibration of airborne UAVSAR for forested area

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Geo-Spatial Information Science 2012年第4期15卷 229-240页

作者： CHENG Xiaoguang naiara PINTO GONG Jianya State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan University129 Luoyu RoadWuhan 430079China Department of Geographical Sciences University of MarylandCollege ParkMD 20770USA NASA/Caltech Jet Propulsion Laboratory PasadenaCAUSA

In the field of biomass estimation,terrain radiometric calibration of airborne polarimetric SAR data for forested areas is an urgent *** area correction ofσ-naught could not completely remove terrain *** by Small and Shimada,this paper tested gamma-naught on one mountainous forested area using airborne Uninhabited Aerial Vehicle Synthetic Aperture Radar data and found it could remove most terrain ***,a systematic increasing trend from far range to near range is found in airborne SAR *** paper made an attempt to use the relationship between distance to SAR sensor andγ-naught to calibrateγ-*** quantitative evaluation methods are *** results demonstrate that variation ofγ-naught can be constrained to a limited extent from near range to far *** this method is based on ground range images,it avoids complicated orthorectification.

关键词： terrain radiometric calibration UAVSAR sigma-naught γ-naught

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World Journal of Psychiatry 2021年第12期11卷 1247-1258页

作者： Joana Jaureguizar Iratxe Redondo Nuria Galende naiara Ozamiz Developmental and Educational Psychology University of the Basque CountryLejona 48940BizkaiaSpain

The coronavirus pandemic has affected all facets of our lives and all ages and social strata *** have been taken to protect against the spread of the virus,such as more rigorous hand hygiene,the use of face masks and social ***,the focus has often been on young people,who have been seen as a group lacking sufficient respect for government-imposed *** review outlines the preventive measures that have been taken in different countries and discusses their specific impact on young people and adolescents,taking into account the developmental stage and concrete needs of this age *** summarizes those studies that have provided information on compliance with preventive measures by young people and adolescents,concluding that although compliance levels among this age group are lower than among older adults,the general view of youths as non-compliant is not consistent with real,objective *** review also summarizes different views regarding the possible reasons for this lower level of compliance,taking into account both social(gender and age)and personal factors(personality,empathy,prosociality,self-control,cognitive styles and motivations),and discusses the practical implications of these findings for the future.

关键词： COVID-19 Health regulations Compliance Young people Adolescents

Simultaneous analysis of the intestinal parasites and diet through eDNA metabarcoding

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Integrative Zoology 2023年第3期18卷 399-413页

作者： Xabier CABODEVILLA Benjamín Juan GÓMEZ-MOLINER naiara ABAD María JoséMADEIRA Department of Zoology and Animal Cell Biology Faculty of PharmacyUniversity of the Basque Country(UPV/EHU)AlavaSpain Instituto de Investigación en Recursos Cinegéticos(IREC)(CSIC-UCLM-JCCM) Ciudad RealSpain

Agricultural expansion and intensiﬁcation are having a huge impact on plant and arthropod diversity and abun-dance,affecting food availability for farmland ***ﬁcult food access,in turn,can lead to immunosuppression and a higher incidence of *** the studies designed to examine changes in the diet of birds and their par-asites,metabarcoding is proving particularly *** technique requires mini-barcodes capable of amplifying the DNA of target organisms from fecal environmental *** help to understand the impact of agricultural expansion on biodiversity,this study sought to design and identify mini-barcodes that might simultaneously as-sess diet and intestinal parasites from the feces of farmland *** capacity to identify diet and parasites of 2 existing and 3 newly developed mini-barcodes was tested“in silico”in relation to the behavior of a reference eukaryotic *** the newly designed mini-barcodes,MiniB18S_81 showed the higher taxonomic cover-age of eukaryotic taxa and a greater ampliﬁcation and identiﬁcation capacity for diet and parasite ***,when it was tested on fecal samples from 5 different steppe bird species,MiniB18S_81 showed high taxonomic resolution of the most relevant diet and parasite phyla,Arthropoda,Nematoda,Platyhelminthes,and Apicomplexa at the order ***,the mini-barcode developed emerges as an excellent tool to simultaneously provide detailed information regarding the diet and parasites of birds,essential for conservation and management.

关键词： biomonitoring broad-spectrum primers eukaryotic mini-barcodes fecal eDNA farmland birds parasitology

Modelling policy combinations of vaccination and transmission suppression of SARS-CoV-2 in Rio de Janeiro,Brazil

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Infectious Disease Modelling 2022年第1期7卷 231-242页

作者： naiara C.M.Valiati Daniel A.M.Villela Universidade Federal Rural do Rio de Janeiro SeropedicaRJBrazil Programa de Computaçao Científica Fundaçao Oswaldo CruzRio de JaneiroRJBrazil

COVID-19 vaccination in Brazil required a phased program,with priorities for age groups,health workers,and vulnerable *** distancing and isolation interventions have been essential to mitigate the advance of the pandemic in several *** developed a mathematical model capable of capturing the dynamics of the SARS-CoV-2 dissemination aligned with social distancing,isolation measures,and *** data from the city of Rio de Janeiro provided a case study to analyze possible scenarios,including non-pharmaceutical interventions and vaccination in the epidemic *** results demonstrate that the combination of vaccination and policies of transmission suppression potentially lowered the number of hospitalized cases by 380þand 66þthousand cases,respectively,compared to an absence of such *** top of transmission suppressiononly policies,vaccination impacted more than 230þthousand averted hospitalized cases and 43þthousand averted ***,health surveillance activities should be maintained along with vaccination planning in scheduled groups until a large vaccinated coverage is ***,this analytical framework enables evaluation of such scenarios.

关键词： SARS-CoV-2 Vaccination COVID-19

NiWO4 powders prepared via polymeric precursor method for application as ceramic luminescent pigments

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Journal of Advanced Ceramics 2020年第1期9卷 55-63页

作者： naiara A.LIMA Lorena D.S.ALENCAR Máximo SIU-LI Carlos A.C.FEITOSA Alexandre MESQUITA Jean-Claude M’PEKO Maria I.B.BERNARDI Instituto de Física de Sao Carlos Universidade de Sao Paulo13560-970 Sao CarlosSPBrazil Instituto Federal de Mato Grosso do Sul IFMS79200-000 AquidauanaMSBrazil Departamento de Física Centro de Ciências Exatas e TecnologiaUniversidade Federal do MaranhaoUFMA65080-805Sao LuisMABrazil Instituto de Geociências e Ciências Exatas Departamento de FísicaUniversidade Estadual Paulista13506-900Rio ClaroSPBrazil

Ni WO4 was prepared using the polymeric precursor method and studied in terms of physical and chemical properties to verify its stability for industrial applications as *** characterization was accomplished using thermal analyses,X-ray diffraction(XRD),scanning electron microscopy(SEM),photoluminescence(PL)and UV–Vis spectroscopies,colorimetric coordinates,and Raman *** the temperature,successive exothermic reactions were observed and they are related with thermal decomposition of the organic *** stability was reached at^700℃.The material is verified to become completely free of second phase at^800℃.The end Ni WO4 powders showed an intense charge transfer(CT)-related tail centered in the ultraviolet region,resulting in a yellow *** addition,the powders exhibited broad excitation band and broad deep blue–green emission band,which were enhanced with increasing powders’crystallinity.

关键词： nickel tungstate yellow pigment colorimetric coordinate optical property structural characterization

Safety of digoxin in nonagenarian patients with atrial fibrillation:lessons from the Spanish Multicenter Registry

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Journal of Geriatric Cardiology 2021年第10期18卷 809-815页

作者： Pablo Domínguez-Erquicia Sergio Raposeiras-Roubín Emad Abu-Assi María Cespón-Fernández David Alonso-Rodríguez Santiago Jesús Camacho-Freire naiara Cubelos-Fernández Álvaro López-Masjuán Ríos María Melendo-Viu AndrésÍñiguez-Romo Department of Cardiology University HospitalÁlvaro CunqueiroVigoSpain Health Research Institute Galicia Sur VigoSpain Department of Cardiology University Hospital of LeónLeónSpain Department of Cardiology University Hospital Juan Ramón JiménezHuelvaSpain

BACKGROUND The association between digoxin and mortality is an unclear *** older patients with atrial fibrillation(AF),where use of digoxin is frequent,the evidence of its safety is *** aim is to assess the safety of digoxin in nonagenarian patients with *** We evaluated data from 795 nonagenarian patients with non-valvular AF from the Spanish Multicenter *** analyzed the relationship between digoxin and all-cause mortality with the Cox proportional-hazards *** Follow-up was 27.7±18.3 *** age was 92.5±3.8 years,and 71%of nonagenarian patients were *** was not associated with increased risk of mortality[adjusted hazard ratio(aHR)=1.16,95%CI:0.96−1.41,P=0.130].However,we found a significant increase in mortality in the subgroup with estimated glomerular filtration rate(eGFR)<30 mL/min per 1.73 m^(2)(aHR=2.01,95%CI:1.13−3.57,P=0.018),but not in the other subgroups of eGFR(30−59 mL/min per 1.73 m^(2) and≥60 mL/min per 1.73 m^(2)).When exploring the risk of mortality according to sex,male subgroup was associated with an in-crease in mortality(aHR=1.48,95%CI:1.02−2.14,P=0.041).This was not observed in females subgroup(aHR=1.03,95%CI:0.81−1.29,P=0.829).Based on the presence or absence of heart failure,we did not find significant differences(aHR=1.20,95%CI:0.87−1.65,P=0.268 ***=1.15,95%CI:0.90−1.47,P=0.273,respectively).CONCLUSIONS In our large registry of nonagenarian patients with AF,we did not find an association between digoxin and mortality in the total ***,in the subgroup analyses,we found an increase in mortality with the use of digoxin in men and in patients with an eGFR<30 mL/min per 1.73 m^(2).

关键词： patients digoxin fibrillation